scholarly journals Production of Phenyllactic Acid from Porphyra Residues by Lactic Acid Bacterial Fermentation

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 678
Author(s):  
Chung-Hsiung Huang ◽  
Wei-Chen Chen ◽  
Yu-Huei Gao ◽  
Hsin-I Hsiao ◽  
Chorng-Liang Pan
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Broad Spectrum ◽  
Algal Biomass ◽  
Phenyllactic Acid ◽  
Bacterial Fermentation ◽  
Fermented Broth ◽  
Valuable Compounds ◽  
De Man ◽  
Mrs Broth

The concept of algae biorefinery is attracting attention because of the abundant valuable compounds within algal biomass. Phenyllactic acid (PhLA), a broad-spectrum antimicrobial organic acid that can be produced by lactic acid bacteria (LAB), is considered a potential biopreservative. In this study, a cascading biorefinery approach was developed to harvest PhLA from Porphyra residues by LAB fermentation. LAB strains were cultivated in de Man, Rogosa and Sharpe (MRS) broth to screen their ability to produce PhLA. As the strains of Lactobacillus plantarum KP3 and L. plantarum KP4 produced higher concentrations of PhLA at 0.09 mg/mL, these two strains were employed for fermentation. After phycobiliprotein extraction, the Porphyra residues, ultrafiltration eluate, phenylalanine (Phe) and yeast extract with a volume of 20 mL were inoculated with LAB strain KP3 and fermented at 37 °C for 120 h. The PhLA content of the fermented broth was 1.86 mg. To optimize the biorefinery process, the ultrafiltration eluate was replaced by commercial cellulase. Up to 4.58 mg of PhLA, which was 2.5 times greater than that produced from KP3 cultured in MRS broth, could be harvested. Taken together, the findings provide an optimized process for LAB fermentation, which is shown to be a feasible algae biorefinery approach to obtaining PhLA from Porphyra residues.

scholarly journals IDENTIFIKASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA ACAR KETIMUN (Cucumis sativus L.) SEBAGAI AGENSI PROBIOTIK

2021 ◽  
Vol 1 (1) ◽  
pp. 31-37
Author(s):  
Febry Surbakti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Reuteri ◽  
Sampling Technique ◽  
Fermented Foods ◽  
Biochemical Tests ◽  
Cucumis Sativus L ◽  
De Man ◽  
Mrs Broth

Di Indonesia terkenal dengan berbagai macam makanan tradisional fermentasi. Jenis makanan tradisional yang banyak dijumpai yang diproses dengan menggunakan proses fermentasi adalah tape, asinan, acar dan lainnya yang mengandung banyak bakteri asam laktat (BAL). Bakteri asam laktat ini merupakan kekayaan alam mikroba yang masih harus dieksplorasi dibidang kesehatannya. Penelitian ini bertujuan untuk mengetahui jenis-jenis dan karakteristik bakteri asam laktat yang terdapat pada acar ketimun. Penelitian ini menggunakan metode deskriptif. Teknik pengambilan sampel langsung dilakukan secara aseptis. Bahan yang digunakan adalah bakteri asam laktat yang diambil dari cairan acar ketimun dengan variasi isi ketimun, ketimun nenas, ketimun nenas dan cabai dan ditumbuhkan pada media MRS Broth (de Man Rogosa Sharpe). Berdasarkan hasil penelitian identifikasi dan karakterisasi bakteri asam laktat pada acar ketimun dengan menggunakan uji morfologi, pewarnaan gram dan uji biokimia maka diperoleh 2 jenis bakteri asam laktat pada acar ketimun. Adapun bakteri asam laktat yang ditemukan antara lain adalah Lactobacillus plantarum dan Lactobacillus reuteri. Karakterisitik secara umum adalah basil, gram positif, katalase negatif, tidak motil, suhu optimum 250°C- 300°C dan memiliki tipe fermentasi heterofermentatif dan homofermentatif.  ABSTRACT: In Indonesia it is famous for various kinds of traditional fermented foods. Traditional food types that are often found that are processed using fermentation are tape, pickled, pickled and others that contain a lot of lactic acid bacteria (LAB). This lactic acid bacteria is a natural wealth of microbes that must still be explored in the field of health. This study aims to determine the types and characteristics of lactic acid bacteria found in pickles to Cucumber. This research uses a descriptive method. The direct sampling technique is carried out aseptically. The material used is lactic acid bacteria taken from pickled liquid to Cucumber with various contents to Cucumber, to Pineapple Cucumber, to Cucumber pineapple and chili and grown on MRS Broth media (de Man Rogosa Sharpe). Based on the results of the study of identification and characterization of lactic acid bacteria on pickles to Cucumber using morphological tests, gram staining and biochemical tests, 2 types of lactic acid bacteria were obtained on pickles to Cucumber. The lactic acid bacteria found include Lactobacillus plantarum and Lactobacillus reuteri. General characteristics are basil, gram positive, negative catalase, not motile, optimum temperature 250C-300C and have heterofermentative and homofermentative fermentation types. Keywords: Lactic acid bacteria, Probiotik, Pickled cucumber

scholarly journals STUDI VIABILITAS ISOLAT BAKTERI ASAM LAKTAT YANG DIISOLASI DARI ASINAN REBUNG BAMBU TABAH TERHADAP pH RENDAH DAN GARAM EMPEDU

2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Nurul Octavia Wasis ◽  
Nyoman Semadi Antara ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Bile Salts ◽  
Salt Resistance ◽  
Low Ph ◽  
Fermented Food ◽  
Bamboo Shoot ◽  
Probiotic Lactic Acid Bacteria ◽  
Mrs Broth

Tabah bamboo shoot pickle is one of the fermented food which is the source of lactic acid bacteria.  Lactic acid bacteria (LAB) is beneficial to health because it has the ability as a probiotic. Lactic acid bacteria that have probiotic criteria should have resistance to low pH and bile salts. This study aims to determine isolates of lactic acid bacteria isolated from tabah bamboo shoot pickle resistant to low pH and bile salts (NaDC). Lactic acid bacteria were tested to low pH by using MRS broth that have different pH (pH 2, pH 3, pH 4 and pH 6.2 as a control) incubated at 37ºC for 3 hours. isolates were survive in low pH then continued in bile salt resistance test with 0.3% bile salt concentration for 15 minutes, 30 minutes, 45 minutes, 60 minutes and 24 hours. The results showed that three isolates out of 88 isolates had ability to grow in low pH and in medium supplemented by NaDC 0,3%. The isolates are AR 3057, AR 3101 and AR 6152 which can be used as candidat of  probiotic. Keywords : Tabah bamboo shoot pickle, lactic acid bacteria, probiotic, low pH, bile salt

scholarly journals UPLC-QTOF-MS/MS and GC-MS Characterization of Phytochemicals in Vegetable Juice Fermented Using Lactic Acid Bacteria from Kimchi and Their Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1761
Author(s):  
Moeun Lee ◽  
Jung Hee Song ◽  
Eun Ji Choi ◽  
Ye-Rang Yun ◽  
Ki Won Lee ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phenyllactic Acid ◽  
Antioxidant Power ◽  
Vegetable Juice ◽  
Daucus Carota L ◽  
Ferric Reducing Antioxidant Power

This study aims to investigate fermentative metabolites in probiotic vegetable juice from four crop varieties (Brassica oleracea var. capitata, B. oleracea var. italica, Daucus carota L., and Beta vulgaris) and their antioxidant properties. Vegetable juice was inoculated with two lactic acid bacteria (LAB) (Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124) isolated from kimchi and their properties were evaluated using untargeted UPLC-QTOF-MS/MS and GC-MS. The samples were also evaluated for radical (DPPH• and OH•) scavenging activities, lipid peroxidation, and ferric-reducing antioxidant power. The fermented vegetable juices exhibited high antioxidant activities and increased amounts of total phenolic compounds. Fifteen compounds and thirty-two volatiles were identified using UPLC-QTOF-MS/MS and GC-MS, respectively. LAB fermentation significantly increased the contents of d-leucic acid, indole-3-lactic acid, 3-phenyllactic acid, pyroglutamic acid, γ-aminobutyric acid, and gluconic acid. These six metabolites showed a positive correlation with antioxidant properties. Thus, vegetable juices fermented with WiKim39 and WiKim0124 can be considered as novel bioactive health-promoting sources.

scholarly journals THE EFFECT OF STORAGE CONDITION ON VIABILITY OF LACTIC ACID BACTERIA IN PROBIOTIC PRODUCT

2018 ◽  
Vol 11 (13) ◽  
pp. 84
Author(s):  
Yuni Trisnawita ◽  
Jansen Silalahi ◽  
Siti Morin Sinaga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Storage Temperature ◽  
Storage Condition ◽  
Significant Loss ◽  
Viability Test ◽  
Probiotic Product ◽  
De Man

Objective: The aim of this study was to determine the effect of storage condition on viability of lactic acid bacteria (LAB) in probiotic product.Methods: Four different of probiotic products used were A (Lacto B), B (Rillus), C (Interlac), and D (Lacbon) containing single or mixed LAB. The product was stored at temperature of 4°C and 28°C for 28 days. Viability test of LAB was done by counting a number of colony bacteria that live on de man, Rogosa, and Sharpe Agar.Results: The results of the study showed that counts of the LAB colonies in product A were less at the label (5.04×107 cfu/sachet), whereas in products B, C, and D were matching with the label. Storage at a temperature of 28°C for 28 days showed significant loss on the viability of LAB in product C (p<0.05).Conclucion: Storage temperature affecting on viability of LAB in probiotic product where storage at temperature 4°C is higher than 28°C for 28 days.

Identification and characterisation of the predominant lactic acid-producing and lactic acid-utilising bacteria in the foregut of the feral camel (Camelus dromedarius) in Australia

2011 ◽  
Vol 51 (7) ◽  
pp. 597 ◽  
Author(s):  
M. B. Ghali ◽  
P. T. Scott ◽  
G. A. Alhadrami ◽  
R. A. M. Al Jassim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Volatile Fatty Acids ◽  
Camelus Dromedarius ◽  
Rrna Gene ◽  
Acid Media ◽  
Butyrivibrio Fibrisolvens ◽  
Prevotella Ruminicola ◽  
De Man

The camel is emerging as a new and important animal in the Australian livestock industry. However, little is known regarding the microbial ecosystem of the gastrointestinal tract of this ruminant-like animal. This study was carried out to determine the diversity of lactic acid-producing and lactic acid-utilising bacteria in the foregut of the feral camel (Camelus dromedarius) in Australia. Putative lactic acid bacteria were isolated from the foregut contents of camels by culturing on De Man, Rogosa, Sharpe and lactic acid media. Identification of representative isolates was based on the analysis of 16S rRNA gene sequences. Fermentation end products of glucose (i.e. volatile fatty acids and lactate) were also measured in vitro. The key predominant bacteria identified in this study were closely related to Streptococcus bovis, Selenomonas ruminantium, Butyrivibrio fibrisolvens, Lachnospira pectinoschiza and Prevotella ruminicola. The main L-lactate producers were those isolates closely related to S. bovis, S. ruminantium and Lactococcus garvieae, while the efficient lactate utilisers were S. ruminantium-related isolates. D-lactate was produced by isolates closely related to either L. pectinoschiza or S. ruminantium. The predominant bacteria isolated and characterised in this study are identical and/or closely related to those typically found in true ruminants (e.g. S. ruminantium, B. fibrisolvens, S. bovis). In addition, some of the bacteria isolated represent novel species of Lachnospira and Clostridium in the context of lactic acid bacteria from a large herbivorous host. The results from this study have contributed to our understanding and provide opportunities to reduce foregut acidosis in the camel.

scholarly journals Metabolite Profiles of Lactic Acid Bacteria in Grass Silage

2007 ◽  
Vol 73 (17) ◽  
pp. 5547-5552 ◽  
Author(s):  
Anders Broberg ◽  
Karin Jacobsson ◽  
Katrin Ström ◽  
Johan Schnürer
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pichia Anomala ◽  
Coumaric Acid ◽  
Phenyllactic Acid ◽  
Liquid Cultures ◽  
Antifungal Metabolites ◽  
Metabolite Profiles ◽  
Bioassay Guided Fractionation ◽  
Test Organisms

ABSTRACT The metabolite production of lactic acid bacteria (LAB) on silage was investigated. The aim was to compare the production of antifungal metabolites in silage with the production in liquid cultures previously studied in our laboratory. The following metabolites were found to be present at elevated concentrations in silos inoculated with LAB strains: 3-hydroxydecanoic acid, 2-hydroxy-4-methylpentanoic acid, benzoic acid, catechol, hydrocinnamic acid, salicylic acid, 3-phenyllactic acid, 4-hydroxybenzoic acid, (trans, trans)-3,4-dihydroxycyclohexane-1-carboxylic acid, p-hydrocoumaric acid, vanillic acid, azelaic acid, hydroferulic acid, p-coumaric acid, hydrocaffeic acid, ferulic acid, and caffeic acid. Among these metabolites, the antifungal compounds 3-phenyllactic acid and 3-hydroxydecanoic acid were previously isolated in our laboratory from liquid cultures of the same LAB strains by bioassay-guided fractionation. It was concluded that other metabolites, e.g., p-hydrocoumaric acid, hydroferulic acid, and p-coumaric acid, were released from the grass by the added LAB strains. The antifungal activities of the identified metabolites in 100 mM lactic acid were investigated. The MICs against Pichia anomala, Penicillium roqueforti, and Aspergillus fumigatus were determined, and 3-hydroxydecanoic acid showed the lowest MIC (0.1 mg ml−1 for two of the three test organisms).

Characterization of the lactic acid bacteria in artisanal dairy products

1997 ◽  
Vol 64 (3) ◽  
pp. 409-421 ◽  
Author(s):  
TIMOTHY M. COGAN ◽  
MANUELA BARBOSA ◽  
ERIC BEUVIER ◽  
BRUNA BIANCHI-SALVADORI ◽  
PIER S. COCCONCELLI ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Broad Spectrum ◽  
Acid Production ◽  
Dairy Products ◽  
Streptococcus Thermophilus ◽  
Listeria Innocua

In all, 4379 isolates from 35 products, including 24 artisanal cheeses, were surveyed with a view to identifying strains that could be used as starters in commercial dairy fermentations. Of the isolates, 38% were classified as Lactococcus, 17% as Enterococcus, 14% as Streptococcus thermophilus, 12% as mesophilic Lactobacillus, 10% as Leuconostoc and 9% as thermophilic Lactobacillus. Acid production by the isolates varied considerably. Of the 1582 isolates of Lactococcus and 482 isolates of mesophilic Lactobacillus tested, only 8 and 2% respectively produced sufficient acid to lower the pH of milk to <5·3 in 6 h at 30°C. In contrast, 53, 32 and 13% of Str. thermophilus, thermophilic Lactobacillus and Enterococcus isolates respectively reduced the pH to 5·3. These isolates were found only in some French, Italian and Greek cheeses. Bacteriocins were produced by 11% of the 2257 isolates tested and 26 of them produced broad-spectrum bacteriocins which inhibited at least eight of the ten target strains used, which included lactic acid bacteria, clostridia and Listeria innocua. The most proteolytic of the 2469 isolates tested were Str. thermophilus from Fontina cheese followed by Enterococcus from Fiore Sardo and Toma cheese and thermophilic Lactobacillus from all sources. Exopolysaccharides were produced by 5·3% of the 2224 isolates tested.

scholarly journals Isolation and Identification of Lactobacillus Bacteria from Culled Hens Meat for Meat Biopreservator

2017 ◽  
Vol 2 (6) ◽  
pp. 518 ◽  
Author(s):  
Hendronoto Arnoldus W. Lengkey ◽  
Roostita Lobo Balia ◽  
Jan Alex Siwi ◽  
Bogdan A. Tasbac ◽  
Iulian Togoe
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antimicrobial Agents ◽  
Carbon Sources ◽  
Gas Production ◽  
Poultry Meat ◽  
Indicator Organisms ◽  
Isolation And Identification ◽  
Lactobacillus Lactis ◽  
Mrs Broth

Using chemical bio-preservatives is being frowned at because of their probable adverse effects on the health of consumers. Isolation and identification of microorganisms from natural resources are an occurring process that have most powerful means for obtaining cultures and also have commercial purposes. The aim of this study was to get bio-preservatives from poultry meat, by isolation and then identification of lactic acid bacteria. Lactic acid bacteria do not pose any health risks to human, and are generally recognized as safe (GRAS) organisms. The lactobacillus were isolated from raw poultry meat by appropriate dilutions with NaCl fisiological, and the decimal dilution were mixed with MRS medium and then incubated at 370C for 48-72 h. Pure cultures were maintained in MRS broth agar at 40C for short term use. Thirty well-isolated colonies were picked up and transferred to MRS broth. Selection of strains was made in agreement with morphology, Gram-stain, viability during storage at 40C and antimicrobial activity, was found twenty isolate. The identification of the cultures was based on the characteristics of the lactobacilli as described in Bergey’s manual of determinative bacteriology, fermentation of different carbon sources, gas production from glucose, growth at different temperatures. For anti-biogram, the isolates were inoculated into MRS broth individually and incubated for 24h. The plates were incubated at 37oC overnight. Resistance was defined as the absence of a growth inhibition zone around the discs. Results indicated that 20 isolate of Lactic acid bacteria were identified: 3 isolates of Lactobacillus fermentum, 2 isolates of Lactobacillus paracasei ssp. paracasei, 5 isolates of Lactobacillus plantarum, 3 isolates of Lactobacillus rhamnosus, 2 isolates of Lactobacillus lactis ssp. lactis 1, and   5 isolates of  Lactobacillus lactis ssp. lactis 2. Characterization of the microbial metabolic product for antimicrobial agents reveals that lactic acid bacteria has responsibility for the inhibition of the indicator organisms, and can be  used as meat biopreservator. Keywords: isolation, identification, lactobacillus, poultry meat, biopreservator.

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