Correction: Roato et al. A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation. Processes 2020, 8, 88

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched of adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSCs yield was measured with flow cytometry after SVF isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through H&E staining and biochemical assay for total collagen, type-2 collagen, and GAGs quantification. Flow cytometry showed a 2-fold increase of ATD-MSCs yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product shows that Lipocell is an efficient method to purify the tissue from undesired byproducts, preserving ATD-MSCs vitality and ECM integrity, resulting in a promising tool for regenerative medicine applications.

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A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

Processes ◽

10.3390/pr8010088 ◽

2020 ◽

Vol 8 (1) ◽

pp. 88

Author(s):

Ilaria Roato ◽

Federico Mussano ◽

Simone Reano ◽

Filippo Boriani ◽

Andrea Margara ◽

...

Keyword(s):

Flow Cytometry ◽

Extracellular Matrix ◽

Adipose Tissue ◽

Regenerative Medicine ◽

Stromal Vascular Fraction ◽

Fold Increase ◽

Human Adipose Tissue ◽

Promising Tool ◽

Filter Size ◽

Tissue Recovery

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched with adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a Class II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSC yield was measured with flow cytometry after stromal vascular fraction (SVF) isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through hematoxylin and eosin (H&E) staining and biochemical assay for total collagen, type-2 collagen, and glycosaminoglycans (GAGs) quantification. Flow cytometry showed a two-fold increase of ATD-MSC yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product showed that Lipocell is an efficient method for purifying the tissue from undesired byproducts and preserving ATD-MSC vitality and extracellular matrix (ECM) integrity, resulting in a promising tool for regenerative medicine applications.

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Injectable SVF-loaded porcine extracellular matrix powders for adipose tissue engineering

RSC Advances ◽

10.1039/c6ra09543g ◽

2016 ◽

Vol 6 (58) ◽

pp. 53034-53042 ◽

Cited By ~ 1

Author(s):

Yongzhou Lu ◽

Chuanlong Jia ◽

Bo Bi ◽

Liang Chen ◽

Yiqun Zhou ◽

...

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Adipose Tissue ◽

Stromal Vascular Fraction ◽

Adipose Tissue Engineering ◽

Novel Method

This study provides a novel method in injectable tissue engineering which contains porcine extracellular matrix (ECM) powder scaffolds and stromal-vascular fraction (SVF) cells.

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701-P: Effects of Exercise on Extracellular Matrix Modifiers in Subcutaneous Adipose Tissue of Obese Adults

Diabetes ◽

10.2337/db20-701-p ◽

2020 ◽

Vol 69 (Supplement 1) ◽

pp. 701-P

Author(s):

PALLAVI VARSHNEY ◽

BENJAMIN J. RYAN ◽

CHIWOON AHN ◽

MICHAEL W. SCHLEH ◽

JEFFREY F. HOROWITZ

Keyword(s):

Extracellular Matrix ◽

Adipose Tissue ◽

Subcutaneous Adipose Tissue ◽

Obese Adults ◽

Subcutaneous Adipose

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14 Characterization of adipose tissue extracellular matrix component mRNA expression during porcine adipogenesis using real-time PCR

Journal of Animal Science ◽

10.1093/jas/skz397.080 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 35-35

Author(s):

Maegan A Reeves ◽

Courtney E Charlton ◽

Terry D Brandebourg

Keyword(s):

Extracellular Matrix ◽

Adipose Tissue ◽

Mrna Expression ◽

Real Time ◽

Real Time Pcr ◽

Collagen Type ◽

Primary Cultures ◽

Collagen Type I ◽

Type I ◽

Matrix Metallopeptidase

Abstract Given adipose tissue is histologically classified as connective tissue, we hypothesized expression of extracellular matrix (ECM) components are significantly altered during adipogenesis. However, little is known about the regulation of the ECM during adipose tissue development in the pig. Therefore, the objective of this study was to characterize expression of ECM components during porcine adipogenesis. Primary cultures of adipose tissue stromal-vascular cells were harvested from 3-day-old neonatal pigs (n=6) and preadipocytes induced to differentiate in vitro for 8 days in the presence of insulin, hydrocortisone, and rosiglitazone. Total RNA was extracted from these cultures on days 0 and 8 post-induction. Real-time PCR was then utilized to determine changes in mRNA expression for collagen type I alpha 1 chain (COL1A), collagen type I alpha 2 chain (COL2A), collagen type I alpha 3 chain (COL3A), collagen type I alpha 4 chain (COL4A), collagen type I alpha 6 chain (COL6A), biglycan, fibronectin, laminin, nitogen-1 (NID1), matrix metallopeptidase 2 (MMP2), matrix metallopeptidase 9 (MMP9), metallopeptidase inhibitor 3 (TIMP3). The mRNA abundances of COL1A, COL3A and MMP2 were significantly downregulated 2.86-fold (P < 0.05), 16.7-fold (P < 0.01) and 3.1-fold (P < 0.05) respectively in day 8 (differentiated) compared to day 0 (undifferentiated) cultures. Meanwhile, mRNA abundances were significantly upregulated during adipogenesis for the COL2A (2.82-fold; P < 0.05), COL4A (2.01-fold; P < 0.05), COL6A (2.8-fold; P < 0.05), biglycan (49.9- fold; P < 0.001), fibronectin (452-fold; P < 0.001), laminin (6.1-fold; P < 0.05), NID1(47.4-fold; P < 0.01), MMP9 (76.8- fold; P < 0.01), and TIMP3(3.04-fold; P < 0.05) genes. These data support the hypothesis that significant changes in ECM components occur during porcine adipogenesis. Modulating adipose tissue ECM remodeling might be a novel strategy to manipulate adiposity in the pig.

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Adipose tissue exposed to high fat diet affects extracellular matrix genes in the mesenchymal stem cell population

Atherosclerosis ◽

10.1016/j.atherosclerosis.2021.06.430 ◽

2021 ◽

Vol 331 ◽

pp. e144

Author(s):

U.T. Arasu ◽

T. Örd ◽

J. Liikkanen ◽

S. Kettunen ◽

T. Lonnberg ◽

...

Keyword(s):

Extracellular Matrix ◽

Adipose Tissue ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cell Population ◽

High Fat Diet ◽

Stem Cell Population ◽

High Fat ◽

Mesenchymal Stem Cell Population

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A novel method to differentiate cell adhesion and aggregation of whole blood on extracellular matrix seeded with endothelial cells

Thrombosis Research ◽

10.1016/0049-3848(86)91433-7 ◽

1986 ◽

Vol 41 ◽

pp. 44

Author(s):

W.G. Eisert ◽

H.H. Callisen ◽

T.H. Mueller

Keyword(s):

Extracellular Matrix ◽

Endothelial Cells ◽

Cell Adhesion ◽

Whole Blood ◽

Differentiate Cell ◽

Novel Method

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Augmentation of Dermal Wound Healing by Adipose Tissue-Derived Stromal Cells (ASC)

Bioengineering ◽

10.3390/bioengineering5040091 ◽

2018 ◽

Vol 5 (4) ◽

pp. 91 ◽

Cited By ~ 5

Author(s):

Joris van Dongen ◽

Martin Harmsen ◽

Berend van der Lei ◽

Hieronymus Stevens

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Adipose Tissue ◽

Stromal Cells ◽

Randomized Clinical Trials ◽

Cell Types ◽

Matrix Remodeling ◽

Skin Wound Healing ◽

Dermal Wound Healing ◽

Dermal Wound

The skin is the largest organ of the human body and is the first line of defense against physical and biological damage. Thus, the skin is equipped to self-repair and regenerates after trauma. Skin regeneration after damage comprises a tightly spatial-temporally regulated process of wound healing that involves virtually all cell types in the skin. Wound healing features five partially overlapping stages: homeostasis, inflammation, proliferation, re-epithelization, and finally resolution or fibrosis. Dysreguled wound healing may resolve in dermal scarring. Adipose tissue is long known for its suppressive influence on dermal scarring. Cultured adipose tissue-derived stromal cells (ASCs) secrete a plethora of regenerative growth factors and immune mediators that influence processes during wound healing e.g., angiogenesis, modulation of inflammation and extracellular matrix remodeling. In clinical practice, ASCs are usually administered as part of fractionated adipose tissue i.e., as part of enzymatically isolated SVF (cellular SVF), mechanically isolated SVF (tissue SVF), or as lipograft. Enzymatic isolation of SVF obtained adipose tissue results in suspension of adipocyte-free cells (cSVF) that lack intact intercellular adhesions or connections to extracellular matrix (ECM). Mechanical isolation of SVF from adipose tissue destructs the parenchyma (adipocytes), which results in a tissue SVF (tSVF) with intact connections between cells, as well as matrix. To date, due to a lack of well-designed prospective randomized clinical trials, neither cSVF, tSVF, whole adipose tissue, or cultured ASCs can be indicated as the preferred preparation procedure prior to therapeutic administration. In this review, we present and discuss current literature regarding the different administration options to apply ASCs (i.e., cultured ASCs, cSVF, tSVF, and lipografting) to augment dermal wound healing, as well as the available indications for clinical efficacy.

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