scholarly journals Solubility of Luteolin and Other Polyphenolic Compounds in Water, Nonpolar, Polar Aprotic and Protic Solvents by Applying FTIR/HPLC

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1952
Author(s):  
Saša Rajhard ◽  
Lucija Hladnik ◽  
Filipa A. Vicente ◽  
Stanko Srčič ◽  
Miha Grilc ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Quantitative Method ◽  
Polyphenolic Compounds ◽  
Ftir Analysis ◽  
Initial Screening ◽  
Beneficial Effects ◽  
Protic Solvents ◽  
Shed Light

In recent years, flavonoids have become a highly researched topic due to their health beneficial effects. Since flavonoids’ solubility plays a significant role in their use in pharmaceutical, food, biological, and chemical areas, the determination of suitable solvents is crucial. Fourier transform infrared (FTIR) analysis was used to characterize functional groups of several flavonoids and phenolic compounds, namely luteolin, hesperidin, quercetin, naringenin, gallic acid and tannic acid. Concentration dependence on transmittance was evaluated for these compounds in ethanol. Afterwards, luteolin was chosen as a model flavonoid, with its concentration correlated with transmittance using 15 solvents with different polarities. Luteolin solubility was further corroborated with high-performance liquid chromatography (HPLC). These results shed light on using FTIR as a semi-quantitative method for the initial screening of solvents and the solubility of different compounds while saving time and solvents. Hence, HPLC would only be needed as a final step for the most promising solvents.

scholarly journals The Determination of Benzo[a]Pyrene and Benz[a]Anthracene in Mainstream and Sidestream Smoke of the Kentucky Reference Cigarette 1R4F and a Cigarette Which Heats but Does Not Burn Tobacco: A Comparison

1991 ◽  
Vol 15 (1) ◽  
pp. 11-17 ◽  
Author(s):  
CH Risner
Keyword(s):  
High Performance Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Quantitative Method ◽  
Normal Phase ◽  
Tobacco Company ◽  
Reverse Phase ◽  
Sidestream Smoke ◽  
Polycyclic Aromatic

AbstractA quantitative method for the determination of benz[a]anthracene (B[a]A) and benzo[a]pyrene (B[a]P) in mainstream and sidestream cigarette smoke by high performance liquid chromatography (HPLC) has been developed. Mainstream and sidestream particulate matter is collected on Cambridge filter pads. The polycyclic aromatic hydrocarbons (PAH) are extracted with cyclohexane and subjected to normal-phase chromatography to isolate target fractions which are concentrated and then diluted with acetonitrile or acetonitrile/water prior to reverse-phase analytical chromatography. The Kentucky Reference cigarette 1R4F and a cigarette which heats but does not burn tobacco (New Cigarette) developed at R.J. Reynolds Tobacco Company were analysed and compared with respect to B[a]P and B[a]A in both mainstream and sidestream smoke.

scholarly journals Determination of some phenolic acids in Algerian propolis

2021 ◽  
Vol 32 (2) ◽  
pp. 120-124
Author(s):  
Abdelkrim Rebiai ◽  
Bachir Ben Seghir ◽  
Hadia Hemmami ◽  
Soumeia Zeghoud ◽  
Mohamed Lakhder Belfar ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Quantitative Method ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Plant Exudates ◽  
North And South

Abstract Propolis is a resinous material collected by bees from various plant exudates, rich in well-known phenolic compounds, such as phenolic acids, that are important to health. Extracts of propolis are very complex matrices that are hard to test. The purpose of this study was to characterize some of the propolis phenolics that were collected from five different districts in Algeria. The High-Performance Liquid Chromatography (HPLC), a modern quantitative method, has been adopted to identify the phenolic acids. Moreover, total phenolic content of four different phenolic acids were identified, with the most abundant being chlorogenic acid, followed by caffeic acid, gallic acid, and p-coumaric acid, the obtained ratios from phenolic acids being in the range of 52.193 to 148.151 μg/g, 0.043 to 7.128 mg/g, 0.328 to 0.440 mg/g and 0.328 to 0.440 mg/g, respectively. Overall, our analysis indicates that all the samples of propolis tested are healthy sources of phenolic acids and the significant differences in the concentrations of the acids were observed for propolis samples from north and south of Algeria. It is probably the effect of different conditions of the collection of the resin and secrets by bees.

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