scholarly journals Acid-free Hydrothermal Process for Synthesis of Bioactive Glasses 70SiO2–(30-x)CaO–xZnO (x = 1, 3, 5 mol.%)

Proceedings ◽  
2020 ◽  
Vol 62 (1) ◽  
pp. 6
Author(s):  
Ta Anh Tuan ◽  
Elena V. Guseva ◽  
Le Hong Phuc ◽  
Nguyen Quan Hien ◽  
Nguyen Viet Long ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Hydrothermal Process ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Bioactive Glasses ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Zn Addition ◽  
Sbf Solution

Bioactive glasses 70SiO2–(30-x)CaO–xZnO (x = 1, 3, 5 mol.%) were prepared by the acid-free hydrothermal method in keeping with green chemical technology. The synthetic glasses were investigated by TG-DSC, BET, XRD, and SEM–EDX methods. All synthetic glasses present mesoporous structures consisting of aggregates of nanoparticles. The bioactivity of synthetic glasses was confirmed through the formation of the hydroxyapatite phase after an in vitro experiment in simulated body fluid (SBF) solution. The effect of Zn addition is shown through the decrease in the bioactivity of synthetic glasses. Additionally, the inductively coupled plasma optical emission spectrometry (ICP-OES) analysis indicates that the Zn ions were released from the glassy networks during in vitro experiments, and they act as Zn(OH)2 suspended precipitation to inhibit the apatite deposition. The in vitro experiment in cell culture matter was performed for SaOS2 and Eahy929 cells. The results confirm the biocompatibility of synthetic glasses and the role of Zn addition in the proliferation of living cells.

scholarly journals Simple and Acid-Free Hydrothermal Synthesis of Bioactive Glass 58SiO2-33CaO-9P2O5 (wt%)

Crystals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 283
Author(s):  
Ta Anh Tuan ◽  
Elena V. Guseva ◽  
Nguyen Anh Tien ◽  
Ho Tan Dat ◽  
Bui Xuan Vuong
Keyword(s):  
Bioactive Glass ◽  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Hydrothermal Process ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Ionic Exchange ◽  
Gel Method ◽  
Sol Gel Method

The paper focuses on the acid-free hydrothermal process for the synthesis of bioactive glass. The new method avoids the use of harmful acid catalysts, which are usually used in the sol-gel process. On the other hand, the processing time was reduced compared with the sol-gel method. A well-known ternary bioactive glass 58SiO2-33CaO-9P2O5 (wt%), which has been widely synthesized through the sol-gel method, was selected to apply to this new process. Thermal behavior, textural property, phase composition, morphology, and ionic exchange were investigated by thermal analysis, N2 adsorption/desorption, XRD, FTIR, SEM, and inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The bioactivity and biocompatibility of synthetic bioactive glass were evaluated by in vitro experiments with a simulated body fluid (SBF) solution and cell culture medium. The obtained results confirmed that the acid-free hydrothermal process is one of the ideal methods for preparing ternary bioactive glass.

scholarly journals P2O5-Free Cerium Containing Glasses: Bioactivity and Cytocompatibility Evaluation

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3267
Author(s):  
Gigliola Lusvardi ◽  
Francesca Sgarbi Stabellini ◽  
Roberta Salvatori
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
X Ray Diffraction ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Doped Glasses ◽  
Plasma Mass Spectrometry ◽  
Sbf Solution ◽  
Xrd Techniques

(1) Background: valuation of the bioactivity and cytocompatibility of P2O5-free and CeO2 doped glasses. (2) Methods: all glasses are based on the Kokubo (K) composition and prepared by a melting method. Doped glassed, K1.2, K3.6 and K5.3 contain 1.2, 3.6, and 5.3 mol% of CeO2. Bioactivity and cytotoxicity tests were carried out in simulated body fluid (SBF) solution and murine osteocyte (MLO-Y4) cell lines, respectively. Leaching of ions concentration in SBF was determined by inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES). The surface of the glasses were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. (3) Results: P2O5-free cerium doped glasses are proactive according to European directives. Cerium increases durability and retards, but does not inhibit, (Ca10(PO4)6(OH)2, HA) formation at higher cerium amounts (K3.6 and K5.3); however, cell proliferation increases with the amount of cerium especially evident for K5.3. (4) Conclusions: These results enforce the use of P2O5-free cerium doped bioactive glasses as a new class of biomaterials.

scholarly journals In Vitro Evaluation of Biologically Derived Hydroxyapatite Coatings Manufactured by High Velocity Suspension Spraying

2021 ◽  
Author(s):  
M. Blum ◽  
M. Sayed ◽  
E. M. Mahmoud ◽  
A. Killinger ◽  
R. Gadow ◽  
...  
Keyword(s):  
High Velocity ◽  
Inductively Coupled Plasma ◽  
Ph Value ◽  
Low Cost ◽  
Optical Emission ◽  
Fish Bone ◽  
Emission Spectrometry ◽  
Slight Variation ◽  
Flame Spray

AbstractThis investigation aims to study a novel biologically derived coating applied on Ti alloy substrates. Obtained from a low-cost fish bone resource, a nanocrystalline hydroxyapatite has been synthesized and converted to an organic suspension. Coating was then manufactured by a high-velocity suspension flame spray process. The microstructure, phase composition, coating thickness, and roughness of hydroxyapatite (HA)-coated samples were studied. The results indicated the presence of both hydroxyapatite and β-tricalcium phosphate phases and the final coating layer was uniform and dense. In vitro bioactivity and biodegradability of the HA/Ti composite samples were estimated by immersion in simulated body fluid. Remarkable reductions in Ca2+ and PO43− ion concentrations were observed as well as low weight loss percentage and a slight variation in the pH value, indicating the generation of an apatite layer on the surface of all studied samples. Scanning electron microscopy, energy-dispersive x-ray analysis, and inductively coupled plasma–optical emission spectrometry confirm these results. Thus biological derived HA coatings are a promising candidate to enhance bioactivity and biodegradability of bone implants. To demonstrate feasibility on commercial medical components, a medical screw was coated and evaluated.

scholarly journals Simplified ICP OES-Based Method for Determination of 12 Elements in Commercial Bottled Birch Saps: Validation and Bioaccessibility Study

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1256
Author(s):  
Maja Welna ◽  
Anna Szymczycha-Madeja ◽  
Pawel Pohl
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Direct Analysis ◽  
Emission Spectrometry ◽  
Dietary Intakes ◽  
Icp Oes ◽  
Element Analysis ◽  
Inductively Coupled

Commercially bottled birch saps (BSs) were analyzed for several nutrient (Ca, Cu, Fe, Mg, and Zn) and toxic (As, Cd, Ni, and Pb) elements using inductively coupled plasma optical emission spectrometry (ICP OES). The method was validated under the conditions of several sample preparation procedures, including a traditional digestion as well as alternative non-digestion schemes. It was found that the direct analysis of untreated BSs gives the best results, i.e., limits of detection at 0.02–5.8 ng mL−1, precision better than 5%, accuracy from 98.0% to 104.5% and determination of 12 elements in a short time (~1 min per sample). The multi-element analysis of nine commercially available bottled BSs showed that they contained mainly Mg and Ca, small quantities of Mn, Zn, Cu, and Fe, but are free from toxic elements such as As, Cd, Ni, and Pb. Additionally, the nutritional value of BSs was examined using in vitro gastro-intestinal digestion (GID) to determine the bioaccessible fraction of elements. Accordingly, bioaccessibility of nutritious ones (Ca, Cu, Fe, Mg, Zn) was <40%. Drinking daily 1 L of BSs covered <2.5% of recommended dietary intakes (RDIs) of the aforementioned elements. Only the bioaccessibility of Mn highly contributes to its RDI.

scholarly journals The comparative absorption of silicon from different foods and food supplements

2009 ◽  
Vol 102 (6) ◽  
pp. 825-834 ◽  
Author(s):  
Supannee Sripanyakorn ◽  
Ravin Jugdaohsingh ◽  
Wacharee Dissayabutr ◽  
Simon H. C. Anderson ◽  
Richard P. H. Thompson ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Positive Control ◽  
Emission Spectrometry ◽  
Orthosilicic Acid ◽  
Green Beans ◽  
Inductively Coupled ◽  
Gastrointestinal Conditions ◽  
Kinetics Of Dissolution

Dietary Si (orthosilicic acid; OSA) appears important in connective tissue health, and although the sources and intakes of Si are well established, its absorption is not. Si absorption was measured from eight high-Si-containing sources: alcohol-free beer; OSA solution (positive control); bananas; green beans; supplemental choline-stabilised OSA (ChOSA); supplemental monomethyl silanetriol (MMST); supplemental colloidal silica (CS); magnesium trisilicate British Pharmacopoeia antacid (MTBP). Two of the supplements and the antacid were pre-selected following an in vitro dissolution assay. Fasting, healthy subjects (CS, n 3; others, n ≥ 5) each ingested two of the sources separated by a 1-week wash-out period. Blood and urine were collected and measured for total Si concentrations by inductively coupled plasma optical emission spectrometry. Absorption, based on urinary Si excretion, was highest for MMST and alcohol-free beer (64 % of dose), followed by green beans (44 %), OSA (43 %), ChOSA (17 %), bananas and MTBP (4 %) and CS (1 %). Peak serum concentrations occurred by 0.5 h for MMST and green beans, 1.5 h for OSA and alcohol-free beer, 2 h for ChOSA and CS, and 4 h for MTBP. Area under the serum curves correlated positively with urinary Si output (r 0·82; P < 0·0001). Absorption of Si from supplements and antacids was consistent with their known chemical speciation and kinetics of dissolution under simulated gastrointestinal conditions. Monomeric silicates were readily absorbed, while particulate silicates were decreasingly well absorbed with increasing polymerisation. The present results highlight the need to allow for relative absorption of Si from different foods or supplements in subsequent epidemiological and intervention studies.

Biocompatible luminescent nanoparticles on the basis of calcium phosphate

2011 ◽  
Vol 1355 ◽  
Author(s):  
Sofia Dembski ◽  
Moritz Milde ◽  
Emeline Dassonneville ◽  
Carsten Gellermann ◽  
Torsten Klockenbring ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Inductively Coupled ◽  
Transmission Electron ◽  
Core Shell Nanoparticles

ABSTRACTLuminescent lanthanide doped SiO2/Hydroxylapatite (HAp) core/shell nanoparticles (NPs) were synthesized by sol-gel technology. The resulting NPs exhibited an amorphous SiO2 core and a crystalline luminescent shell. The formation of the HAp layer was possible at pH 8.5. The characterization of the resulting NPs was done by transmission electron microscopy, X-ray diffraction analysis, inductively-coupled plasma combined with optical emission spectrometry, and photoluminescence spectroscopy. Additionally, the newly developed SiO2/HAp:Ln3+ core/shell NPs were tested for their biocompatibility, e. g. by an in vitro cell culture based assay.

Effect of Carbonate Substitution on the Biomineralization of Apatite Bulk and Coating

2019 ◽  
Vol 807 ◽  
pp. 87-93
Author(s):  
Qing Xia Zhu ◽  
Quan Yi Nie
Keyword(s):  
Grain Size ◽  
Pore Size ◽  
Carbonate Apatite ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Carbonate Substitution ◽  
Experiment Method ◽  
Mineralization Processes ◽  
Sbf Solution

In-vitro experiment method was used to study biomineralization properties of carbonate apatite bulk and electrophoresis coating with different carbonate contents. The mineralization processes in SBF solution were investigated by means of XRD, SEM and so on. Research results show that the apatite with poor crystallization and small grain size induced by the carbonate substitution is beneficial to the sintering of the bulk and the formation of the bone-like hydroxyl-carbonate apatite (HCA) layer on the surface after soaking in the SBF. However, the carbonate substitution greatly reduces the pore size and porosity of coating, which is not conducive to the deposition and growth of the mineralized layer, thus weakens the biomineralization properties of the coating.

scholarly journals Bioaccessibility Assessment of Cu, Fe, K, Mg, P, and Zn in Thermally Treated Lamb Meat

2021 ◽  
Author(s):  
Julymar de Higuera ◽  
Herick Santos ◽  
Aline de Oliveira ◽  
Ana Rita Nogueira
Keyword(s):  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Cooking Methods ◽  
Inductively Coupled ◽  
Lamb Meat ◽  
Temperature Loss ◽  
Meat Samples

A bioaccessibility test with raw and cooked lamb meat samples was performed. The evaluated cooking devices were grill, microwave oven, air fryer, pressure cooker, and electric oven. Physicochemical parameters and the total mass fraction of Cu, Fe, K, Mg, P, and Zn were determined in raw and cooked samples by inductively coupled plasma optical emission spectrometry (ICP OES). The trueness was evaluated using certified reference materials, with recoveries from 87 to 101%. The pressure cooking presented the major changes, including the highest values of internal temperature, loss of inorganic elements after cooking, and the lowest values of moisture and analyte mass fractions. An in vitro gastrointestinal simulation was performed, and the method was validated by an addition and recovery test, in which the trueness varied from 87 to 115%. The bioaccessibility ranged between 28-56, 4-19, 68-76, 41-54, 48-57, and 1-21% for Cu, Fe, K, Mg, P, and Zn, respectively. The cooking methods promoted changes in the meat samples, thus affecting the bioaccessibility of the nutrients. Based on the recommended dietary intake (RDI) calculation, lamb meat can be considered a good Fe, P, and Zn source.

Sign in / Sign up

Export Citation Format

Share Document