scholarly journals Methane Production from Alginate-Extracted and Non-Extracted Waste of Laminaria japonica: Anaerobic Mono- and Synergetic Co-Digestion Effects on Yield

2019 ◽  
Vol 11 (5) ◽  
pp. 1269 ◽  
Author(s):  
Mohammed Osman ◽  
Shao Xiaohou ◽  
Deling Zhao ◽  
Amir Basheer ◽  
Hongmei Jin ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Synergetic Effect ◽  
Value Added ◽  
Laminaria Japonica ◽  
Ammonia Nitrogen ◽  
System Stability ◽  
Coefficient Of Determination ◽  
Biogas Yield

This study investigated the potentiality of methane production from alginate-extracted (AEWLJ) and non-extracted (NAEWLJ) waste of Laminaria japonica through batch anaerobic fermentation in mono- and co-digestion with rice straw (RS) at different mixing ratios. Optimal C/N ratio was demonstrated, and system stability was monitored in terms of the total ammonia nitrogen, total volatile fatty acids, and pH throughout the digestion period. The results show that the combination of AEWLJ/RS at 67% mixing ratio generated the highest biogas yield of 247 NmL/gVS, which was 36% higher than the AEWLJ alone. The synergetic effect was clearly observed leading to an increase in the total methane yield up to 78% and 88%, respectively, for arrays of AEWLJ/RS and NAEWLJ/RS. The kinetic model showed a high coefficient of determination (R2 ≥ 0.9803) when the modified Gompertz model was applied to predict methane production. These outcomes support the possibility of an integrated biorefinery approach to attain value-added products in order to achieve circular economies.

Sludge accumulation and methanogenic activity in an anaerobic lagoon

2000 ◽  
Vol 42 (10-11) ◽  
pp. 247-255 ◽  
Author(s):  
J. Paing ◽  
B. Picot ◽  
J. P. Sambuco ◽  
A. Rambaud
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Anaerobic Degradation ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Methanogenic Activity ◽  
Start Up ◽  
Anaerobic Lagoon ◽  
Methane Production Rate ◽  
Sludge Accumulation

Sludge accumulation and the characteristics of anaerobic digestion in sludge had been investigated in a primary anaerobic lagoon. Methanogenic potential of sludge was evaluated by an anaerobic digestion test which measured the methane production rate. Sludge was sampled at several points in the lagoon to determine spatial variations and with a monthly frequency from the start-up of the lagoon to observe the development of anaerobic degradation. Maximum amounts of sludge accumulated near the inlet. The mean methane production of sludge was 2.9 ml gVS–1 d–1. Sludge near the outlet presented a greater methanogenic activity and a lesser concentration of volatile fatty acids than near the inlet. The different stages of anaerobic degradation were spatially separated, acidogenesis near the inlet and methanogenesis near the outlet. This staged distribution seemed to increase efficiency of anaerobic fermentation compared with septic tanks. Methane release at the surface of the lagoon was estimated to be very heterogeneous with a mean of 25 l m–2 d–1. The development of performance and sludge characteristics showed the rapid beginning of methanogenesis, three months after the start-up of the anaerobic lagoon. Considering the volume of accumulated sludge, it could however be expected that methanogenic activity would further increase.

Effect of a Cellulose-Degrading Strain on Anaerobic Fermentation of Corn Straw

2011 ◽  
Vol 356-360 ◽  
pp. 2510-2514 ◽  
Author(s):  
Ming Fen Niu ◽  
Sai Yue Wang ◽  
Wen Di Xu ◽  
An Dong Ge ◽  
Hao Wang
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Corn Straw ◽  
High Efficient ◽  
Important Significance ◽  
Strong Capacity

In order to improve the rate of degradation of cellulose in corn straw, the study has an important significance that compost corn straw with inoculating high-efficient microbe agents. The experiment inoculated a cellulose-degrading strain F2 which was screened from compost into compost pretreatment, the VS of corn straw reduced from 93.14% to 71.69% after 15 days, the content of cellulose reduced from 34.12g·kg-1 to 25.66g·kg-1, the rate of degradation was 24.79% which was 10.60% higher than those without the strain. An anaerobic fermentation experiment was carried out with the two groups of composted corn straw and mixed pig feces with a certain ratio, and investigations of biogas production, pH, content of volatile fatty acids(VFA) and rate of methane production were conducted. The results were that the corn straw composted with the cellulose-degrading strain peaked 4 days earlier, the maximal daily biogas production was 1470mL, the cumulative biogas production reached 23641mL which was 16.87% higher and operated stably earlier. The study showed that the cellulose-degrading strain had a strong capacity to degrade cellulose in corn straw, and then improved the performance of anaerobic digestion.

scholarly journals Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro

2020 ◽  
Vol 8 (8) ◽  
pp. 1160 ◽  
Author(s):  
Jiangkun Yu ◽  
Liyuan Cai ◽  
Jiacai Zhang ◽  
Ao Yang ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Optimal Dose ◽  
Illumina Miseq ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Rumen Microbiota

This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.

scholarly journals Insects as Novel Ruminant Feed and a Potential Mitigation Strategy for Methane Emissions

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2648
Author(s):  
Eslam Ahmed ◽  
Naoki Fukuma ◽  
Masaaki Hanada ◽  
Takehiro Nishida
Keyword(s):  
Fatty Acids ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Acheta Domesticus ◽  
Environmental Benefit ◽  
Grass Hay

This study is the first to evaluate the chemical composition and impacts of four different edible insects, Acheta domesticus (A.d), Brachytrupes portentosus (B.p), Gryllus bimaculatus (G.b), and Bombyx mori (B.m), on the digestibility, rumen fermentation, and methane production when used as a substitute for 25% of the soybean meal (SBM) in a ruminant diet through in vitro incubation. The dietary treatments were 100% grass hay, 60% grass hay + 40% SBM, 60% grass hay + 30% SBM + 10% A.d, 60% grass hay + 30% SBM + 10% B.p, 60% grass hay + 30% SBM + 10% G.b, and 60% grass hay + 30% SBM + 10% B.m. The experiment was conducted as a short-term batch culture for 24 h at 39 °C, and the incubation was repeated in 3 consecutive runs. Chemical analysis of the insects showed that they were rich in fat (14–26%) with a high proportion of unsaturated fatty acids (60–70%). Additionally, the insects were rich in protein (48–61%) containing all essential amino acids and the amino acid profiles of the insects were almost the same as that of SBM. The inclusion of insects did not affect nutrient digestibility or the production of volatile fatty acids but did increase the production of ammonia-nitrogen. The addition of G.b and B.m led to decrease in methane production by up to 18% and 16%, respectively. These results reveal that substitution of 25% SBM in the diet with the tested insects had no negative impacts, and their potential to reduce methane production is an environmental benefit.

scholarly journals Biogas production of Chicken Manure by Two-stage fermentation process

2018 ◽  
Vol 38 ◽  
pp. 01048 ◽  
Author(s):  
Xin Yuan Liu ◽  
Jing Jing Wang ◽  
Jia Min Nie ◽  
Nan Wu ◽  
Fang Yang ◽  
...  
Keyword(s):  
Methane Production ◽  
Fermentation Process ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Chicken Manure ◽  
Lag Phase ◽  
Two Stage ◽  
Peak Period ◽  
Production Stage

This paper performs a batch experiment for pre-acidification treatment and methane production from chicken manure by the two-stage anaerobic fermentation process. Results shows that the acetate was the main component in volatile fatty acids produced at the end of pre-acidification stage, accounting for 68% of the total amount. The daily biogas production experienced three peak period in methane production stage, and the methane content reached 60% in the second period and then slowly reduced to 44.5% in the third period. The cumulative methane production was fitted by modified Gompertz equation, and the kinetic parameters of the methane production potential, the maximum methane production rate and lag phase time were 345.2 ml, 0.948 ml/h and 343.5 h, respectively. The methane yield of 183 ml-CH4/g-VSremoved during the methane production stage and VS removal efficiency of 52.7% for the whole fermentation process were achieved.

scholarly journals Effects of Mixing Volatile Fatty Acids as Carbon Sources on Rhodospirillum rubrum Carbon Metabolism and Redox Balance Mechanisms

2021 ◽  
Vol 9 (9) ◽  
pp. 1996
Author(s):  
Paloma Cabecas Segura ◽  
Quentin De Meur ◽  
Audrey Tanghe ◽  
Rob Onderwater ◽  
Laurent Dewasme ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Carbon Metabolism ◽  
Volatile Fatty Acids ◽  
Rhodospirillum Rubrum ◽  
Anaerobic Fermentation ◽  
Purple Bacteria ◽  
Carbon Sources ◽  
Value Added ◽  
Production Costs

Rhodospirillum rubrum has a versatile metabolism, and as such can assimilate a broad range of carbon sources, including volatile fatty acids. These carbon sources are gaining increasing interest for biotechnological processes, since they reduce the production costs for numerous value-added compounds and contribute to the development of a more circular economy. Usually, studies characterizing carbon metabolism are performed by supplying a single carbon source; however, in both environmental and engineered conditions, cells would rather grow on mixtures of volatile fatty acids (VFAs) generated via anaerobic fermentation. In this study, we show that the use of a mixture of VFAs as carbon source appears to have a synergy effect on growth phenotype. In addition, while propionate and butyrate assimilation in Rs. rubrum is known to require an excess of bicarbonate in the culture medium, mixing them reduces the requirement for bicarbonate supplementation. The fixation of CO2 is one of the main electron sinks in purple bacteria; therefore, this observation suggests an adaptation of both metabolic pathways used for the assimilation of these VFAs and redox homeostasis mechanism. Based on proteomic data, modification of the propionate assimilation pathway seems to occur with a switch from a methylmalonyl-CoA intermediate to the methylcitrate cycle. Moreover, it seems that the presence of a mixture of VFAs switches electron sinking from CO2 fixation to H2 and isoleucine production.

scholarly journals The Efficacy of Mootral Supplementation on Methane Production and Rumen Fermentation Characteristics in Ruminants Fed Different Styles

2021 ◽  
Author(s):  
Eslam Ahmed ◽  
Naoki Fukuma ◽  
Masaaki Hanada ◽  
Takehiro Nishida
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Carbon Dioxide Production ◽  
Methane Emissions ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Fiber Digestibility ◽  
Methane Reduction ◽  
Dietary Treatments ◽  
Natural Combination

Abstract Background: Using natural feed supplements to mitigate methane emissions from ruminants is a promising strategy. Many antimethanogenic compounds have been used to alter rumen fermentation, yet their potential to reduce methane production effectively is not consistent across different kinds of feeding styles (forage:concentrate ratios). Therefore, this study was conducted to investigate the impacts of Mootral (MT), a natural combination of garlic powder and bitter orange extract, on methane production, rumen fermentation, and digestibility in different feeding models commonly used for ruminants. The dietary treatments were 1000 g grass/kg ration (10 GRS), 8 GRS + 200 g concentrate/kg ration (2CON), 6GRS + 4CON, 4GRS + 6CON, and 2GRS + 8CON. MT was supplemented at 200 g/kg of the feed. Each group consisted of 6 replicates. The experiment was performed as a batch culture for 24 h at 39 °C. This procedure was repeated in 3 consecutive runs. Results: The results of this experiment showed that supplementation with MT strongly reduced methane production in all kinds of feeding models (P<0.001). Its efficacy in reducing methane/digestible dry matter was 44% in the 10GRS diet, and this reductive power increased with the inclusion of CON up to a 69.5% reduction with the 2GRS + 8CON diet. MT application significantly increased gas and carbon dioxide production and the concentration of ammonia-nitrogen, but decreased the pH (P<0.001). In contrast, it did not interfere with organic matter and fiber digestibility. Supplementation with MT was effective in altering rumen fermentation toward less acetate and more propionate and butyrate. Additionally, it improved the production of total volatile fatty acids in all feeding models (P<0.001). Conclusions: The MT combination showed effective methane reduction by improving rumen fermentation characteristics without exhibiting adverse effects on fiber digestibility. Thus, MT could be used with all kinds of feeding models to effectively mitigate methane emissions from ruminants.

scholarly journals The Efficacy of Plant-Based Bioactives Supplementation to Different Proportion of Concentrate Diets on Methane Production and Rumen Fermentation Characteristics In Vitro

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1029
Author(s):  
Eslam Ahmed ◽  
Naoki Fukuma ◽  
Masaaki Hanada ◽  
Takehiro Nishida
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
In Vitro Study ◽  
Carbon Dioxide Production ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Fiber Digestibility ◽  
Methane Reduction ◽  
The Impact

This In Vitro study was conducted to investigate the impact of plant-bioactives extract (PE), a combination of garlic powder and bitter orange extract, on methane production, rumen fermentation, and digestibility in different feeding models. The dietary treatments were 1000 g grass/kg ration + 0 g concentrate/kg ration (100:0), 80:20, 60:40, 40:60, and 20:80. The PE was supplemented at 200 g/kg of the feed. Each group consisted of 6 replicates. The experiment was performed as an In Vitro batch culture for 24 h at 39 °C. This procedure was repeated in three consecutive runs. The results of this experiment showed that supplementation with PE strongly reduced methane production in all kinds of feeding models (p < 0.001). Its efficacy in reducing methane/digestible dry matter was 44% in the 100:0 diet, and this reduction power increased up to a 69.2% with the inclusion of concentrate in the 20:80 diet. The PE application significantly increased gas and carbon dioxide production and the concentration of ammonia-nitrogen, but decreased the pH (p < 0.001). In contrast, it did not interfere with organic matter and fiber digestibility. Supplementation with PE was effective in altering rumen fermentation toward less acetate and more propionate and butyrate (p < 0.001). Additionally, it improved the production of total volatile fatty acids in all feeding models (p < 0.001). In conclusion, the PE combination showed effective methane reduction by improving rumen fermentation characteristics without exhibiting adverse effects on fiber digestibility. Thus, PE could be used with all kinds of feeding models to effectively mitigate methane emissions from ruminants.

Anaerobic Fermentation of Combined Chicken Manure and Straw on a Sequencing Batch Reactor: Analysis of Stability and Numerical Modeling

2021 ◽  
Vol 15 (5) ◽  
pp. 621-628
Author(s):  
Lei Feng ◽  
Huisong Gu ◽  
Xiaofei Zhen
Keyword(s):  
Methane Production ◽  
Logistic Model ◽  
Anaerobic Fermentation ◽  
Batch Reactor ◽  
Cellulase Activity ◽  
Ammonia Concentration ◽  
Chicken Manure ◽  
System Stability ◽  
Methane Yield ◽  
Analysis Of Stability

Anaerobic fermentation experiments were performed using combinations of chicken manure and straw at a temperature of 37±0.5 °C for 70 d. In this investigation, system stability and methane production were analyzed using the Logistic model. According to our results, the highest cumulative methane yield was 292.87 mL/g VS at a straw ratio of 3%. This value was 17.43% higher than the one obtained using pure chicken manure. In addition, a positive correlation between ammonia concentration and content of chicken manure was observed. At the end of the reaction, pH values in the four groups were between 7.0 and 8.0. However, pH in the M2 group was significantly higher than that observed in the rest of the groups. Data also indicated that hydrolase activities were positively correlated with SCOD concentrations. In this context, cellulase activity reached the highest value on day 40. Proteinase activity presented two peaks on days 20 and 40. In the case of lipase, activities and amylase initially increased and later decreased, but the change is small. According to the results of the Logistic model, the highest methane production potential was of 404.41 mL/g VS, at a straw proportion of 3%. In addition, the highest daily methane yield was 6.13 mL/g VS.

scholarly journals Anaerobic Co-Digestion of Kitchen Waste and Blackwater for Different Practical Application Scenarios in Decentralized Scale: From Wastes to Energy Recovery

Water ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2556
Author(s):  
Huihui Wang ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Xuemei Wang ◽  
Siqi Zuo
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Rural Areas ◽  
Biogas Production ◽  
Nitrogen Concentration ◽  
Synergetic Effect ◽  
Ammonia Nitrogen ◽  
Kitchen Waste ◽  
Total Ammonia Nitrogen ◽  
Total Ammonia

This study was performed to investigate the anaerobic digestion feasibility of kitchen waste and blackwater under different scenarios in laboratory tests. According to biochemical methane potential tests, when the kitchen waste to blackwater solid ratio was 1:1, the cumulative methane production reached the highest amount at 313.2 mL/g volatile solids (VSs), which was 26.4% and 29.4% higher than the anaerobic monodigestion of kitchen waste and blackwater, respectively, indicating that the anaerobic codigestion of kitchen waste and blackwater had a synergetic effect. Furthermore, the effect of different initial total ammonia nitrogen concentrations in blackwater on anaerobic digestion was determined based on the above experimental results, thereby proving that reducing the total ammonia nitrogen concentration in blackwater can appropriately improve the efficiency of methane production. Therefore, anaerobic digestion is a suitable method for the biogas production of kitchen waste and blackwater. It is of great significance for the organic waste stream treatment of households in a decentralized scale, especially in rural areas.

Sign in / Sign up

Export Citation Format

Share Document