scholarly journals Inhibition during Anaerobic Co-Digestion of Aqueous Pyrolysis Liquid from Wastewater Solids and Synthetic Primary Sludge

2020 ◽  
Vol 12 (8) ◽  
pp. 3441 ◽  
Author(s):  
Saba Seyedi ◽  
Kaushik Venkiteshwaran ◽  
Nicholas Benn ◽  
Daniel Zitomer
Keyword(s):  
Methane Production ◽  
Oxygen Demand ◽  
Community Analysis ◽  
Gas Production ◽  
Microbial Community Analysis ◽  
Pyrolysis Gas ◽  
Primary Sludge ◽  
Bio Oil ◽  
Concomitant Reduction ◽  
Pyrolysis Liquid

Pyrolysis can convert wastewater solids into useful byproducts such as pyrolysis gas (py-gas), bio-oil and biochar. However, pyrolysis also yields organic-rich aqueous pyrolysis liquid (APL), which presently has no beneficial use. Autocatalytic pyrolysis can beneficially increase py-gas production and eliminate bio-oil; however, APL is still generated. This study aimed to utilize APLs derived from conventional and autocatalytic wastewater solids pyrolysis as co-digestates to produce biomethane. Results showed that digester performance was not reduced when conventional APL was co-digested. Despite having a lower phenolics concentration, catalyzed APL inhibited methane production more than conventional APL and microbial community analysis revealed a concomitant reduction in acetoclastic Methanosaeta. Long-term (over 500-day) co-digestion of conventional APL with synthetic primary sludge was performed at different APL organic loading rates (OLRs). Acclimation resulted in a doubling of biomass tolerance to APL toxicity. However, at OLRs higher than 0.10 gCOD/Lr-d (COD = chemical oxygen demand, Lr = liter of reactor), methane production was inhibited. In conclusion, conventional APL COD was stoichiometrically converted to methane in quasi steady state, semi-continuous fed co-digesters at OLR ≤ 0.10 gCOD/Lr-d. Undetected organic compounds in the catalyzed APL ostensibly inhibited anaerobic digestion. Strategies such as use of specific acclimated inoculum, addition of biochar to the digester and pretreatment to remove toxicants may improve future APL digestion efforts.

scholarly journals Co-digestion of hybrid Pennisetum and peanut shell after adding TiO2 nanoparticles: Focusing on the synergistic effects on methane production

2021 ◽  
Author(s):  
HongMei Zhao ◽  
Meng Jiang
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Synergistic Effects ◽  
Community Analysis ◽  
Gas Production ◽  
Microbial Community Analysis ◽  
Peanut Shell ◽  
Hybrid Pennisetum ◽  
Mesophilic Conditions

Abstract Anaerobic digestion is a widely accepted method to treat wastes such as peanut shell. The energy and nutrients are simultaneously recovered by this method. The objective of this study was to elucidate the effect of TiO 2 nanoparticles in co-digestion of hybrid Pennisetum and peanut shell under mesophilic conditions. The results demonstrated the methane (CH 4 ) production was improved by adding the TiO 2 nanoparticles. The cumulative gas production is best (up to 11,133.3 mL) by adding 0.15% nano-TiO 2 particles. The microbial community analysis showed that Methanobacterium and Methanosarcina were enriched in the presence of TiO 2 nanoparticles indicating that TiO 2 can improve CH 4 production by stimulating the growth of methanogens.

scholarly journals Microbial Community Analysis Using MiSeq Sequencing and Pathway of Methane Production in Tehran WWTP: A Full-Scale Anaerobic Digester

2020 ◽  
Author(s):  
Reza Barati Rashvanlou ◽  
Mahdi Farzadkia ◽  
Abbas Ali Moserzadeh ◽  
Asghar Riazati ◽  
Chiang Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Community Analysis ◽  
Full Scale ◽  
Microbial Community Analysis ◽  
Miseq Sequencing ◽  
Sequencing Method ◽  
Community Data ◽  
Rank Abundance ◽  
Made In

Introduction: One of biological wastewater treatment methods that utilizes to both digesting waste activated sludge and methane production is anaerobic digestion (AD). It is believed to be most effective solution in terms of energy crisis and environmental pollution issues. Materials and Methods: In this study the sludge was digested anaerobically sampled from a full-scale WWTP, located at south of Tehran, Iran for evaluation. To study the microbial community within the sludge the MiSeq Sequencing method utilized. Based on our field data (data not shown) and microbial community data, a schematic diagram of probable leading pathways was made in the studied digester. Results: At first, the community variety in the bulk sludge and richness were enhanced followed by loading increasing. Meanwhile, the loading change enhanced the community richness and variety of the sludge. By comparing the rank-abundance distributions, a shallow gradient would show high evenness since the abundances of diverse species are alike. The results showed all the communities were extremely diverse and 15 phyla were distinguished in the sludge sample. The dominant phyla of the community were Bacteroidetes and Firmicutes and quantity of the two phyla were 21% and 11%, respectively. Anaerobaculum, Acinetobacter, Syntrophomonas, and Coprothermobacter were the chief genera for the microbial communities and the sum of four genera were 7%, 3%, 3%, and 2%, respectively. Conclusion: It was shown that syntrophic acetate oxidizing bacterias (SAOBs) metabolized acetate through hydrogen trophic methanogenesis in the digester. Generally, the findings may be useful to help the wastewater operators to utilize an effective method that able to treat waste sludge plus methane production, simultaneously.

scholarly journals Simultaneous nitrification and denitrification (SND) bioaugmentation with Pseudomonas sp. HJ3 inoculated for enhancing phenol and nitrogen removal in coal gasification wastewater

2019 ◽  
Vol 80 (8) ◽  
pp. 1512-1523
Author(s):  
Weiwei Ma ◽  
Yuxing Han ◽  
Wencheng Ma ◽  
Hongjun Han ◽  
Chunyan Xu ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Coal Gasification ◽  
Oxygen Demand ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Simultaneous Nitrification And Denitrification ◽  
Pseudomonas Sp ◽  
Coal Gasification Wastewater ◽  
Nitrification And Denitrification

Abstract A simultaneous nitrification and denitrification (SND) bioaugmention system with Pseudomonas sp. HJ3 inoculated was established to explore the potential of simultaneous phenol and nitrogen removal in coal gasification wastewater (CGW). When the concentration of influent chemical oxygen demand (COD) and total phenols (TPh) was 1,765.94 ± 27.43 mg/L and 289.55 ± 10.32 mg/L, the average removal efficiency of COD and TPh at the stable operating stage reached 64.07% ± 0.76% and 74.91% ± 0.33%, respectively. Meanwhile, the average removal efficiency of NH4+-N and total nitrogen (TN) reached 67.96% ± 0.17% and 57.95% ± 0.12%, respectively. The maximum SND efficiency reached 83.51%. Furthermore, SND bioaugmentation performed with good nitrification tolerance of phenol shock load and significantly reduced toxic inhibition of organisms. Additionally, the microbial community analysis indicated that Pseudomonas sp. HJ3 was the predominant bacterium in the SND bioaugmentation system. Moreover, the indigenous nitrogen removal bacteria such as Thauera, Acidovorax and Stenotrophomonas were enriched, which further enhanced the nitrogen removal in the SND bioaugmentation system. The results demonstrated the promising application of SND bioaugmentation for enhancing simultaneous phenol and nitrogen removal in CGW treatment.

scholarly journals Evaluation of acidogenic sludge from anaerobic reactors running at low solids retention times to reduce sludge generation and enhance biogas production

Water SA ◽  
2019 ◽  
Vol 45 (4 October) ◽  
Author(s):  
Wilza Da Silva Lopes ◽  
Ysa Helena Diniz Morais de Luna ◽  
Jose Tavares de Sousa ◽  
Wilton Silva Lopes ◽  
Valderi Duarte Leite
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Kinetic Constant ◽  
Oxygen Demand ◽  
Gas Production ◽  
Primary Sludge ◽  
Anaerobic Reactors ◽  
Solids Retention ◽  
Improved Performance ◽  
Pre Treatment

ABSTRACT   Sludges generated in the biological processing of sewage are complex mixtures, the constituents of which pose risks to public health and the environment. Anaerobic digestion is considered the most sustainable option for treating sludge because it offers the possibility of generating biogas. The aim of this study was to compare the quantities, properties, biodegradabilities and biochemical methane potentials (BMP) of primary sludge (PS) generated by a primary decanter with acidogenic sludges produced by upflow anaerobic (UA) reactors operating at solids retention times (SRTs) of 2, 4, 6 and 8 days (Samples S2, S4, S6 and S8, respectively). Sludges from both pre-treatments were submitted to alkaline solubilization in order to determine the efficiency of the process in disrupting extracellular complexes. Based on the levels of total solids (TS) present, the primary decanter was found to generate higher quantities of excess sludge (yield of 3.1 gTS∙d-1) than UA reactors operating at low SRTs (yields in the range 1.69 to 0.64 gTS∙d-1). The concentrations of dissolved materials in PS and Samples S2 and S8 were considerably higher after alkaline solubilization, with respective increases of 8, 14 and 28-fold in dissolved organic carbon, 12, 20 and 40-fold in chemical oxygen demand, 25, 31 and 59-fold in proteins, and 17, 21 and 63-fold in carbohydrates. In addition, the BMP value for S8 was some 13% higher than that recorded for PS while the kinetic constant for gas production by S8 was 1.8-fold greater than that of PS. It is concluded that a pre-treatment combining anaerobic digestion at low SRT and alkaline solubilisation would lead to improved performance in subsequent stages of anaerobic digestion and, consequently, increased efficiency in biogas production.

Development of slow sponge sand filter (SpSF) as a post-treatment of UASB-DHS reactor effluent treating municipal wastewater

2016 ◽  
Vol 74 (1) ◽  
pp. 65-72 ◽  
Author(s):  
N. Maharjan ◽  
K. Kuroda ◽  
K. Dehama ◽  
M. Hatamoto ◽  
T. Yamaguchi
Keyword(s):  
Municipal Wastewater ◽  
Oxygen Demand ◽  
Community Analysis ◽  
Total Coliform ◽  
Post Treatment ◽  
Pollutant Removal ◽  
Microbial Community Analysis ◽  
Anaerobic Sludge ◽  
Sand Filter ◽  
Removal Efficiencies

In this study, conventional slow sand filter (SSF) and modified slow sponge sand filter (SpSF) were investigated for the post-treatment of up-flow anaerobic sludge blanket (UASB)-down-flow hanging sponge (DHS) reactor effluent. The seasonal variation did not show significant differences in removal efficiencies of both filters. However in summer, both filters were able to achieve high total suspended solids and total biochemical oxygen demand removal averaging 97% and 99%, respectively. Contrary to organic removal, total nitrogen removal efficiency was satisfactory, showing increased removal efficiencies averaging 58% and 62% for SSF and SpSF in summer. On the other hand, average total coliform removal of SSF and SpSF was 4.2 logs and 4.4 logs and corresponding Escherichia coli removal was 4.0 logs and 4.1 logs, respectively. From our observation, it could be concluded that the relative performance of SpSF for nutrients and coliforms was better than SSF due to the effectiveness of sponge media over fine sands. Moreover, microbial community analysis revealed that the members of phylum Proteobacteria were predominant in the biofilms of both filters, which could have contributed to pollutant removal. Therefore, SpSF could be concluded to be a suitable post-treatment of UASB-DHS system in warmer conditions.

Methane production and microbial community analysis in the goethite facilitated anaerobic reactors using algal biomass

Fuel ◽  
2015 ◽  
Vol 145 ◽  
pp. 196-201 ◽  
Author(s):  
Juan Tan ◽  
Jin Wang ◽  
Jun Xue ◽  
Shao-Yang Liu ◽  
Shu-Chuan Peng ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Algal Biomass ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Anaerobic Reactors

scholarly journals Bio-Gaseous Fuels from Agricultural Waste Pyrolysis (Part II)

2019 ◽  
Vol 290 ◽  
pp. 11005 ◽  
Author(s):  
Cora Bulmău ◽  
Gabriela Ionescu ◽  
Cosmin Mărculescu
Keyword(s):  
Batch Reactor ◽  
Agricultural Waste ◽  
Gas Production ◽  
Gaseous Species ◽  
Pyrolysis Gas ◽  
Heating Value ◽  
Gaseous Fuels ◽  
Rape Straw ◽  
Bio Oil ◽  
Low Temperature Pyrolysis

The results presented in the following paper are making the aim of a broadly research concentrated to investigate the temperature effects on agricultural waste pyrolysis performed in a batch reactor. Briefly, the motivation along with experimental features and main results generated from the rape straw low temperature pyrolysis (300°C-500°C) have been offered in the first part submitted at the MSE 2019 conference, with the title: Bio-gaseous fuels from agricultural waste pyrolysis (Part I). The current section (second part of the study) presents the results obtained in case of the rape straw high temperature pyrolysis (600°C-800°C). Overall, as expected, the augmentation of the operating temperature, inhibits the bio-oil and biochar formation, enhancing the pyrolysis gas production. The distribution of gaseous components varies depending on temperature and residence time. The transition stages and the formation of the main pyrolysis gaseous species are also presented and discussed. The most dominant chemical element from the pyrolysis gas is N2, due to its constant presence as non-oxidant agent in the process. Considering the same premise, the rape straw pyrolysis gaseous species distribution in the temperature range of 600°C-800°C varies between: 47%-58% CO2, 18%-28% CO, 14%-35% H2, while the pyrolysis gas density 1.1-1.4 kg/m3 and higher heating value 23-52 MJ/kg.

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