scholarly journals Production, Purification, and Characterization of Bacillibactin Siderophore of Bacillus subtilis and Its Application for Improvement in Plant Growth and Oil Content in Sesame

2021 ◽  
Vol 13 (10) ◽  
pp. 5394
Author(s):  
S. Nithyapriya ◽  
Sundaram Lalitha ◽  
R. Z. Sayyed ◽  
M. S. Reddy ◽  
Daniel Joe Dailin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Tamil Nadu ◽  
Agar Plate ◽  
Iron Chelator ◽  
Growth And Yield ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Siderophores are low molecular weight secondary metabolites produced by microorganisms under low iron stress as a specific iron chelator. In the present study, a rhizospheric bacterium was isolated from the rhizosphere of sesame plants from Salem district, Tamil Nadu, India and later identified as Bacillus subtilis LSBS2. It exhibited multiple plant-growth-promoting (PGP) traits such as hydrogen cyanide (HCN), ammonia, and indole acetic acid (IAA), and solubilized phosphate. The chrome azurol sulphonate (CAS) agar plate assay was used to screen the siderophore production of LSBS2 and quantitatively the isolate produced 296 mg/L of siderophores in succinic acid medium. Further characterization of the siderophore revealed that the isolate produced catecholate siderophore bacillibactin. A pot culture experiment was used to explore the effect of LSBS2 and its siderophore in promoting iron absorption and plant growth of Sesamum indicum L. Data from the present study revealed that the multifarious Bacillus sp. LSBS2 could be exploited as a potential bioinoculant for growth and yield improvement in S. indicum.

scholarly journals Endophytic Burkholderia sp. SSG as a potential biofertilizer promoting boxwood growth

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9547
Author(s):  
Ping Kong ◽  
Chuanxue Hong
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Plant Protection ◽  
Growth Promotion ◽  
Agar Plate ◽  
Gene Clusters ◽  
Plant Weight ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background Burkholderia sp. SSG is a bacterial endophyte isolated from boxwood leaves showing a resistant response to infection by the boxwood blight pathogen Calonectria pseudonaviculata. SSG acted as a protective and curative biocontrol agent for boxwood blight and as a bio-sanitizer of disease inoculum in the field. Many gene clusters involved in antibiotic production and plant growth promotion (PGP) were found in the genome, giving this endophyte great application potential as a treatment for plant protection. However, the PGP features have not been documented. This study investigated the plant growth promotion activity of SSG in boxwood. Methods To determine whether SSG is a plant growth promoting bacterium, four PGP traits, auxin and siderophore production, nitrogen fixation and phosphate solubilization, were examined in the laboratory with colorimetric or agar plate assays. The plant growth promoting activity of SSG was tested on three boxwood varieties characterized by slow, intermediate and fast growth rates, namely Justin Brouwers, Buddy and Winter Gem, respectively. These plants were drenched with an SSG cell suspension or water and washed plant weight was compared before and after treatment to determine growth changes after 10 months. Results The SSG culture was sustainable on nitrogen free media, suggesting that SSG may fix atmospheric nitrogen. It was also a strong phosphate solubilizer and a potent siderophore and indole-3-acetic acid (IAA) producer. Significant growth promotion was observed on boxwood cultivars Justin Brouwers, Buddy and Winter Gem 10 months after plant roots were drenched with SSG cells. The growth rate of treated plants was 76.1, 58.3, and 37.3% higher than that of the control, respectively. The degree of growth promotion was significantly different among plant varieties, notably more pronounced with the slow and intermediate growers. This study demonstrates that the SSG bacterium has multiple PGP traits and is a prospective plant biofertilizer.

Biochemical and molecular characterization of DAPG-producing plant growth-promoting rhizobacteria (PGPR) of groundnut (Arachis hypogaea L.)

2016 ◽  
Author(s):  
D. Sherathia ◽  
R. Dey ◽  
M. Thomas ◽  
T. Dalsania ◽  
K. Savsani ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Plant Growth ◽  
Fungal Pathogens ◽  
Quantitative Estimation ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria (PGPR) thrive in the rhizosphere of plants and play a beneficial role in plant growth, and development along with biocontrol activities. The present study was undertaken with the aim of developing rhizobacterial inoculants for groundnut for enhancement of growth and yield and suppression of major soil-borne fungal diseases caused by Sclerotium rolfsii (stem rot) and Aspergillus niger (collar rot). Out of a total of 154 rhizobacterial isolates obtained from groundnut rhizosphere, 78 isolates were selected on the basis of in vitro antifungal activities against three major soil-borne fungal pathogens of groundnut, i.e. Aspergillus niger, Aspergillus flavus and Sclerotium rolfsii. The selected isolates were further screened for the production of 2,4-Diacetylphloroglucinol (2,4-DAPG) by the gene specific PCR amplification of phlD gene. A total of 11 rhizobacterial isolates were found to have DAPG-producing genes and selected for further studies. Gene specific primers were also used for characterization of the isolates for plant growth-promoting and biocontrol traits. The qualitative and quantitative estimation of the various attributes of the isolates were also carried out. Majority of the isolates showed production of IAA, siderophores and fluorescent pigments. The DAPG-producing rhizobacterial isolates have great potential as bio-inoculants for groundnut crop for suppressing soil-borne fungal pathogens and to enhance growth and yield.

Characterization of Root-Endophytic Actinobacteria From Cactus (Opuntia Ficus-Indica) for Plant Growth Promoting Traits

2021 ◽  
Author(s):  
V Govindasamy ◽  
Priya George ◽  
S V Ramesh ◽  
P Sureshkumar ◽  
Jagadish Rane ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Seedling Length ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Endophytic Actinobacteria

Abstract The present study is the first report of isolation and characterization of endophytic actinobacteria from cactus (Opuntia ficus-indica). A total of 179 morphologically distinct endophytic actinobacterial isolates were purified from the roots of two different genetic accessions of cactus. All these isolates were screened for their plant growth promotion traits namely growth on N-free medium, P-solubilization, siderophore production, ACC deaminase activity and IAA production. A majority of the endophytic actinobacterial isolates (85%) exhibited the potential for plant growth promotion under in vitro conditions. Ten among the isolates were selected based on their multi-PGP traits and were identified as Streptomyces sp. based on the 16S rRNA gene sequencing and phylogenetic analysis. Plant growth promoting potential of these selected endophytic Streptomyces was studied in wheat seedlings. All these selected isolates significantly enhanced the growth parameters like seedling length and rootlets number compared to the uninoculated control. The wheat seeds inoculated with Streptomyces tuirus VL-70-IX exhibited maximum number of rootlets (6.33) compared to uninoculated control (3.67). The inoculation of endophytic actinobacteria Streptomyces pseudogriseolus VL-70-XII caused maximum seedling length (20.53 cm) and root length (8.26 cm) while the inoculation of S. radiopugnans HV-VIII resulted in highest shoot length (12.33 cm). These endophytic actinobacteria isolated from the roots of cactus accessions showed potential PGP traits. This work lays foundation for characterization and selection of endophytic actinobacteria from the under-exploited, drought tolerant species such as cactus with potential cross-compatibility for the improvement of plant growth of field crops especially under abiotic stress conditions..

scholarly journals Characterization of Rhizobium and Plant Growth Promoting Rhizobacteria from French Bean Rhizosphere and Their Effect on French Bean Productivity

2021 ◽  
Author(s):  
Saroj Kumar Yadav ◽  
Kiran P. Raverkar
Keyword(s):  
Plant Growth ◽  
Grain Yield ◽  
Carbon Sources ◽  
Plant Growth Promoting Rhizobacteria ◽  
French Bean ◽  
Growth And Yield ◽  
Common People ◽  
Plant Growth Promoting ◽  
Growth Promoting

French bean (Phaseolus vulgaris L.) is used profusely by the common people as an alternative diet of protein. The sparse nodulation in French bean mainly may be due to lack of threshold level of specific rhizobial cells in soil at the time of sowing. The isolates streaked on YEMA with BTB changed to yellow color showing the production of acid which is the characteristic of Rhizobium. Utilization of different carbon sources is an efficient tool to characterize the isolates. Plant growth promoting rhizobacteria is the beneficial rhizobacteria inoculation of which increases growth and yield of French bean through different direct and indirect mechanisms. Inoculation of French beans with rhizobial and rhizobacterial isolates found to be improved growth, physiological, quality parameters and grain yield through symbiotic N2-fixation capacity and plant growth promoting abilities. Co-inoculation of rhizobial and rhizobacterial isolates enhanced the growth and grain yield of French bean. These isolates may be used as consortium to improve the growth of French bean, which may reduce the dependency of farmer on chemical fertilizer as well as risk of pollution. In this chapter characterization of Rhizobium and plant growth promoting rhizobacteria and their effect on plant growth has been reviewed.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

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