scholarly journals Morphology of Dried Drop Patterns of Saliva from a Healthy Individual Depending on the Dynamics of Its Surface Tension

Surfaces ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 395-414 ◽  
Author(s):  
Lyudmila V. Bel’skaya ◽  
Elena A. Sarf ◽  
Anna P. Solonenko

Background: The study of processes in the drying drops of biological fluids (dried drop patterns) and the method of dynamic surface tensiometry can be attributed to integral methods of assessing the state of the organism. Research objective: to establish the relationship between the type of crystallization patterns and the surface tension of human saliva in normal conditions. Methods: 100 volunteers (40 males, 60 females) that were aged 30–59 participated in the study. In all saliva samples, the parameters of dynamic tensiometry, types of crystallization patterns and 11 biochemical parameters were determined. Results: No statistically significant differences in the saliva crystallization patterns were observed, depending on the age and gender characteristics of the volunteers. A negative correlation of the area of the crystallization zone and the surface tension of saliva is shown. When considering the crystallization patterns, their considerable variability was noted; on this basis, the entire studied sample was divided into four clusters by surface tension. Conclusion: In general, the crystallization patterns that are inside the selected groups remain quite heterogeneous. This increases the likelihood of making an incorrect diagnosis when using visual methods to evaluate the crystallization patterns, which significantly limits the use of such diagnostic methods in clinical practice.

2020 ◽  
Vol 74 (5) ◽  
pp. 536-543 ◽  
Author(s):  
Lyudmila V. Bel’skaya ◽  
Elena A. Sarf ◽  
Denis V. Solomatin

The comparison of the characteristics of the infrared (IR) spectra of saliva of healthy volunteers was carried out based on gender and age. It is shown that statistically significant differences between male and female groups are observed for the absorption bands of proteins and lipids. At the same time, the absorbance of the bands assigned to proteins and nucleic acids is higher for males, whereas the absorbance of the bands assigned to lipids is higher in the group of females. It is established that the correlation relationships of the characteristics of the spectra and age are weakly expressed. Thus, when forming the criteria of the norm and pathology for saliva, it is necessary to take into account the gender of the subjects, while there are no strict requirements for taking into account age periodization. Nevertheless, the revealed patterns are valid only for the composition of the saliva of healthy volunteers, the extension of the results to groups of patients with various diseases, as well as other biological fluids, requires additional testing.


1998 ◽  
Vol 44 (3) ◽  
pp. 7-10
Author(s):  
O. V. Sinyachenko ◽  
V. N. Kazakov ◽  
V. B. Faynerman ◽  
R. Miller ◽  
N. D. Cholangot ◽  
...  

Dynamic surface tension (ST) of the blood serum and urine was studied in patients with diabetic nephropathy by the method of maximal pressure in a vesicle using MPT2 computer tensiometer (LAUDA, Germany). The angle of the slope of blood tensiogram curve increased and urinary ST decreased at t = 1 sec in patients with type I diabetics in comparison with those with type II condition. The development of the nephrotic syndrome and chronic renal insufficiency was associated with decrease of ST in the range of short (t = 0.01 sec) and medium (t = 1 sec) times of surface life and of the equilibrium (static) ST (at t -> go/. The parameters of interphase tensiometry correlated with the content of protein and lipid surfactants in biological fluids.


Author(s):  
S.YU. ZAITSEV

Основой метода межфазной тензиометрии является измерение динамического поверхностного натяжения (ДПН) биологических жидкостей человека и животных. В данной работе предложено использование метода регрессии в моделировании взаимосвязи биохимических и ДПН параметров сыворотки крови коров. Показана возможность определения количества общего белка в сыворотке крови телок по известным параметрам тензиограмм поверхностного натяжения на границе раздела жидкость/воздух. В результате измерений 96 проб сыворотки крови телок трех возрастов (6, 12 и 1718 мес) и обработки полученных данных были определены следующие диапазоны значений ДПН: 072,173,9 мН/м, 171,973,5 мН/м, 266,870,1 мН/м, 361,264,4 мН/м, 03,165,96 мНм-1с-1/2, 15,707,34 мНм-1с-1/2. При включении полученных экспериментальных данных ДПН в новое регрессионное уравнение получены следующие значения содержания общего белка: 72,4 г/л 66,7 г/л 69,5 г/л (для телок 6, 12 и 1718 мес), соответственно. Эти значения на 2,5 выше 1,8 и 1,4 ниже, чем соответствующие значения общего белка в сыворотке крови телок, измеренные традиционными биохимическими методами.Данная модель для показателя общего белка имеет хорошее качество (P0,05) и готова к использованию. Регрессионный анализ с использованием простого экспресс-метода межфазной тензиометрии позволяет избавиться от необходимости постоянных измерений показателей общего белка в сыворотке крови телок биохимическими методами.The basis of the method of interfacial tensiometry is the measurement of the dynamic surface tension (DST) of biological fluids in humans and animals. In this paper, the application of the regression method in modeling the relationship of biochemical and DST parameters of cow blood serum is proposed. The possibility of determining the amount of total protein in the blood serum of heifers by the known parameters of tensiograms of surface tension at the liquid/air interface is shown. As a result of measurements of the 96 blood serum samples of heifers of three ages (6, 12 and 1718 months) and processing the obtained data, the following ranges of DST values were determined: 072.173.9 mN/m, 171.973.5 mN/m, 266.870.1 mN/m, 361.264.4 mN/m, 03.165.96 mNm-1s-1/2, 15.707.34 mNm-1s-1/2. When checking the obtained new regression equation according to the serum DST, the following values of the total protein content were obtained: 72.4 g/l 66.7 g/l 69.5 g/l (for heifers of 6, 12 and 1718 months), respectively. These values are about 2.5 higher, 1.8 and 1.4 lower than the corresponding total protein values measured by the traditional biochemical methods. Thus, this model for the indicator of total protein is of good quality (P0.05) and is ready for use. Regression analysis using a simple express-method of interfacial tensiometry allows you to eliminate the need for continuous measurements of total protein in the blood serum of heifers by biochemical methods.


2021 ◽  
Vol 5 (3) ◽  
pp. 37
Author(s):  
Hernán Martinelli ◽  
Claudia Domínguez ◽  
Marcos Fernández Leyes ◽  
Sergio Moya ◽  
Hernán Ritacco

In the search for responsive complexes with potential applications in the formulation of smart dispersed systems such as foams, we hypothesized that a pH-responsive system could be formulated with polyacrylic acid (PAA) mixed with a cationic surfactant, Gemini 12-2-12 (G12). We studied PAA-G12 complexes at liquid–air interfaces by equilibrium and dynamic surface tension, surface rheology, and X-ray reflectometry (XRR). We found that complexes adsorb at the interfaces synergistically, lowering the equilibrium surface tension at surfactant concentrations well below the critical micelle concentration (cmc) of the surfactant. We studied the stability of foams formulated with the complexes as a function of pH. The foams respond reversibly to pH changes: at pH 3.5, they are very stable; at pH > 6, the complexes do not form foams at all. The data presented here demonstrate that foam formation and its pH responsiveness are due to interfacial dynamics.


RSC Advances ◽  
2015 ◽  
Vol 5 (11) ◽  
pp. 7921-7931 ◽  
Author(s):  
S. I. Arias ◽  
J. R. Fernández ◽  
L. García-Rio ◽  
J. C. Mejuto ◽  
M. C. Muñiz ◽  
...  

An axisymmetric model accounts for dynamic surface tension of non-ionic surfactants under consideration of diffusive adsorption behaviour with a finite diffusion length.


2002 ◽  
Vol 244 (1-2) ◽  
pp. 81-86 ◽  
Author(s):  
Romána Zelkó ◽  
Á Orbán ◽  
K Süvegh ◽  
Z Riedl ◽  
I Rácz

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