scholarly journals Biocatalyzed Sulfoxidation in Presence of Deep Eutectic Solvents

2020 ◽  
Vol 1 (3) ◽  
pp. 290-297
Author(s):  
Gonzalo de Gonzalo
Keyword(s):  
Deep Eutectic Solvents ◽  
Optically Active ◽  
Cofactor Regeneration ◽  
Natural Deep Eutectic Solvents ◽  
Phosphite Dehydrogenase ◽  
Valuable Compounds ◽  
First Time ◽  
Substrate Concentrations

The flavin-containing monooxygenase from Methylophaga sp. strain SK1 (mFMO) is a valuable biocatalyst for the preparation of optically active sulfoxides, among other valuable compounds. In this study, we explored to benefits of using Natural Deep Eutectic Solvents (NADESs) when doing oxidation with this biocatalyst, fused to phosphite dehydrogenase for cofactor regeneration (PTDH-mFMO). It was found that optically active sulfoxides could be obtained with slightly higher conversions in 10% v/v NADES when working at substrate concentrations of 50–200 mM, whereas there was no loss in the enantioselectivity. With these results, it is demonstrated for the first time that flavin-containing monooxygenases can be employed as biocatalysts in presence of NADESs.

scholarly journals Efficacy of Natural Deep Eutectic Solvents for Extraction of Hydrophilic and Lipophilic Compounds from Fucus vesiculosus

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4198
Author(s):  
Ekaterina D. Obluchinskaya ◽  
Olga N. Pozharitskaya ◽  
Lyubov V. Zakharova ◽  
Anna V. Daurtseva ◽  
Elena V. Flisyuk ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Deep Eutectic Solvents ◽  
Fucus Vesiculosus ◽  
Lipophilic Compounds ◽  
Natural Deep Eutectic Solvents ◽  
Biological Tests ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
The Impact ◽  
First Time

The impact of the composition of natural deep eutectic solvents (NADES) and extraction conditions on the simultaneous extraction of hydrophilic ascorbic acid (AA), phlorotannins (TPhC), and lipophilic fucoxanthin (FX) from Fucus vesiculosus was investigated for the first time. In biological tests, the NADES extracts showed the promising ability to scavenge DPPH radicals. A positive correlation was observed between DPPH scavenging activity and AA, TPhC, and FX contents. We calculate the synergistic effect of antioxidants extracted by NADES from F. vesiculosus based on the mixture effect (ME). The addition of 30% water to the NADES and the prolongation of sonication time from 20 min up to 60 min were favorable for the ME. The ME for extracts with the NADES was increased by two folds (ME > 2). In contrast, conventional extraction by maceration with steering at 60 °C does not lead to the synergistic effect (ME = 1). It is notable that the NADES provides high stability and preserves the antioxidant activity of the extracts from F. vesiculosus during storage.

A review of sustainable lignocellulose biorefining applying (natural) deep eutectic solvents (DESs) for separations, catalysis and enzymatic biotransformation processes

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ana Bjelić ◽  
Brigita Hočevar ◽  
Miha Grilc ◽  
Uroš Novak ◽  
Blaž Likozar
Keyword(s):  
Special Section ◽  
Cost Effective ◽  
Deep Eutectic Solvents ◽  
Reaction Conditions ◽  
Co Catalysts ◽  
Biomass Fractionation ◽  
Functional Step ◽  
Natural Deep Eutectic Solvents ◽  
Economic Need ◽  
Molecular Compounds

AbstractConventional biorefinery processes are complex, engineered and energy-intensive, where biomass fractionation, a key functional step for the production of biomass-derived chemical substances, demands industrial organic solvents and harsh, environmentally harmful reaction conditions. There is a timely, clear and unmet economic need for a systematic, robust and affordable conversion method technology to become greener, sustainable and cost-effective. In this perspective, deep eutectic solvents (DESs) have been envisaged as the most advanced novel polar liquids that are entirely made of natural, molecular compounds that are capable of an association via hydrogen bonding interactions. DES has quickly emerged in various application functions thanks to a formulations’ simple preparation. These molecules themselves are biobased, renewable, biodegradable and eco-friendly. The present experimental review is providing the state of the art topical overview of trends regarding the employment of DESs in investigated biorefinery-related techniques. This review covers DESs for lignocellulosic component isolation, applications as (co)catalysts and their functionality range in biocatalysis. Furthermore, a special section of the DESs recyclability is included. For DESs to unlock numerous new (reactive) possibilities in future biorefineries, the critical estimation of its complexity in the reaction, separation, or fractionation medium should be addressed more in future studies.

scholarly journals Natural deep eutectic solvents as biofilm structural breakers

2021 ◽  
pp. 117323
Author(s):  
Maria F. Nava-Ocampo ◽  
Lamya Al Fuhaid ◽  
Robert Verpoorte ◽  
Young Hae Choi ◽  
Mark C.M. van Loosdrecht ◽  
...  
Keyword(s):  
Deep Eutectic Solvents ◽  
Natural Deep Eutectic Solvents

scholarly journals Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 582
Author(s):  
Inês Mansinhos ◽  
Sandra Gonçalves ◽  
Raquel Rodríguez-Solana ◽  
José Luis Ordóñez-Díaz ◽  
José Manuel Moreno-Rojas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Industrial Applications ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasonic Assisted

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.

Natural Deep Eutectic Solvents: From Their Discovery to Their Applications

2019 ◽  
pp. 61-81 ◽  
Author(s):  
Henni Vanda ◽  
Robert Verpoorte ◽  
Peter G. L. Klinkhamer ◽  
Young H. Choi
Keyword(s):  
Deep Eutectic Solvents ◽  
Natural Deep Eutectic Solvents
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