scholarly journals Efficacy of Natural Deep Eutectic Solvents for Extraction of Hydrophilic and Lipophilic Compounds from Fucus vesiculosus

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4198
Author(s):  
Ekaterina D. Obluchinskaya ◽  
Olga N. Pozharitskaya ◽  
Lyubov V. Zakharova ◽  
Anna V. Daurtseva ◽  
Elena V. Flisyuk ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Deep Eutectic Solvents ◽  
Fucus Vesiculosus ◽  
Lipophilic Compounds ◽  
Natural Deep Eutectic Solvents ◽  
Biological Tests ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
The Impact ◽  
First Time

The impact of the composition of natural deep eutectic solvents (NADES) and extraction conditions on the simultaneous extraction of hydrophilic ascorbic acid (AA), phlorotannins (TPhC), and lipophilic fucoxanthin (FX) from Fucus vesiculosus was investigated for the first time. In biological tests, the NADES extracts showed the promising ability to scavenge DPPH radicals. A positive correlation was observed between DPPH scavenging activity and AA, TPhC, and FX contents. We calculate the synergistic effect of antioxidants extracted by NADES from F. vesiculosus based on the mixture effect (ME). The addition of 30% water to the NADES and the prolongation of sonication time from 20 min up to 60 min were favorable for the ME. The ME for extracts with the NADES was increased by two folds (ME > 2). In contrast, conventional extraction by maceration with steering at 60 °C does not lead to the synergistic effect (ME = 1). It is notable that the NADES provides high stability and preserves the antioxidant activity of the extracts from F. vesiculosus during storage.

scholarly journals Biocatalyzed Sulfoxidation in Presence of Deep Eutectic Solvents

2020 ◽  
Vol 1 (3) ◽  
pp. 290-297
Author(s):  
Gonzalo de Gonzalo
Keyword(s):  
Deep Eutectic Solvents ◽  
Optically Active ◽  
Cofactor Regeneration ◽  
Natural Deep Eutectic Solvents ◽  
Phosphite Dehydrogenase ◽  
Valuable Compounds ◽  
First Time ◽  
Substrate Concentrations

The flavin-containing monooxygenase from Methylophaga sp. strain SK1 (mFMO) is a valuable biocatalyst for the preparation of optically active sulfoxides, among other valuable compounds. In this study, we explored to benefits of using Natural Deep Eutectic Solvents (NADESs) when doing oxidation with this biocatalyst, fused to phosphite dehydrogenase for cofactor regeneration (PTDH-mFMO). It was found that optically active sulfoxides could be obtained with slightly higher conversions in 10% v/v NADES when working at substrate concentrations of 50–200 mM, whereas there was no loss in the enantioselectivity. With these results, it is demonstrated for the first time that flavin-containing monooxygenases can be employed as biocatalysts in presence of NADESs.

scholarly journals Preparation and Antioxidant Activity of Chitosan Dimers with Different Sequences

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 366
Author(s):  
Wentong Hao ◽  
Kecheng Li ◽  
Yuzhen Ma ◽  
Rongfeng Li ◽  
Ronge Xing ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Vital Role ◽  
Degree Of Polymerization ◽  
Amino Groups ◽  
Structure Activity ◽  
Degree Of Acetylation ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
First Time

As a popular marine saccharide, chitooligosaccharides (COS) has been proven to have good antioxidant activity. Its antioxidant effect is closely related to its degree of polymerization, degree of acetylation and sequence. However, the specific structure–activity relationship remains unclear. In this study, three chitosan dimers with different sequences were obtained by the separation and enzymatic method, and the antioxidant activity of all four chitosan dimers were studied. The effect of COS sequence on its antioxidant activity was revealed for the first time. The amino group at the reducing end plays a vital role in scavenging superoxide radicals and in the reducing power of the chitosan dimer. At the same time, we found that the fully deacetylated chitosan dimer DD showed the strongest DPPH scavenging activity. When the amino groups of the chitosan dimer were acetylated, it showed better activity in scavenging hydroxyl radicals. Research on COS sequences opens up a new path for the study of COS, and is more conducive to the investigation of its mechanism.

scholarly journals Flavonoids from the Roots of Amomum compactum Soland Ex Maton (Zingiberaceae)

2021 ◽  
Vol 7 (2) ◽  
pp. 142-149
Author(s):  
Deden Indra Dinata ◽  
Rani Maharani ◽  
Fauzan Zein Muttaqien ◽  
Unang Supratman ◽  
Mohamad Nurul Azmi ◽  
...  
Keyword(s):  
Chemical Structure ◽  
2D Nmr ◽  
Scavenging Activity ◽  
Endemic Plants ◽  
Spectroscopy Data ◽  
Chemical Structures ◽  
Dpph Scavenging Activity ◽  
Amomum Compactum ◽  
Dpph Scavenging ◽  
First Time

Amomum compactum Soland Ex Maton is one of the Zingiberaceae family plants which is the endemic plants from West Java, Indonesia. This study was aimed to determine the chemical structure of flavonoid compounds from n-hexane extract of A.compactum Sol. Ex Maton roots. Dried powder of the roots was extracted consecutively with n-hexane, ethyl acetate, and methanol solvents. Three flavonoids, 5-hydroxy-3,7,4`-trimethoxy kaempferol (1), 5-hydroxy-3,7,3',4'-tetra methoxy kaempferol (2) and 4'-hydroxy-3,5,7-trimethoxy kaempferol (3), have been isolated from the roots of A. compactum Sol. Ex Maton.  The chemical structures of compounds 1-3 were identified by spectroscopy data including infrared 1D-NMR, 2D-NMR and HRTOF-MS as well as by comparison with previously reported spectral data. Compounds 1-3 were isolated from this plant for the first time and showed free radical DPPH scavenging activity.

scholarly journals Valorization of Fig (Ficus carica Linn.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System For Online Screening And Identification of Antioxidant Compounds

2021 ◽  
Author(s):  
Chunying Li ◽  
Meiting Yu ◽  
Shen Li ◽  
Xue Yang ◽  
Bin Qiao ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ficus Carica ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Screening And Identification ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
First Time

Abstract Fig (Ficus carica Linn.) leaves are produced each year and often thrown away as wastes, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity, however, the antioxidant components are not clear. So as to take full advantage of fig waste leaves, antioxidant capacity of different extracts (petroleum ether, ethyl acetate and water) was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) and ferric-ion reducing antioxidant power (FRAP) methods. The results showed that flavonoids in ethyl acetate extraction had the highest content, 83.92 ± 0.01 mg/g, and maximum DPPH scavenging activity (IC50 0.54 mg/mL), highest ABTS scavenging rate (80.28%), FRAP (3.46 mmol/g). Furthermore, HPLC-QTOF-MS/MS-DPPH method was developed for the first time to identify 11 flavonoids in fig waste leaves. This rapid and efficient method can not only be used for screening the antioxidant components in fig waste leaves, but also can be combined with mass spectrometry to identify the compounds with antioxidant capacity. There are three flavonoids with significant antioxidant capacity, which are 3-O-(rhamnopyranosyl-glucopyranosyl)-7-O-(glucopyranosyl)-quercetin, isoschaftoside and rutin. The results confirmed that fig waste leaves contain a variety of antioxidant components, which contributed to increase the value of fig waste leaves as antioxidants.

scholarly journals Metabolomics of Pigmented Rice Coproducts Applying Conventional or Deep Eutectic Extraction Solvents Reveal a Potential Antioxidant Source for Human Nutrition

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 110
Author(s):  
Millena Cristina Barros Santos ◽  
Nathalie Barouh ◽  
Erwann Durand ◽  
Bruno Baréa ◽  
Mélina Robert ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Extraction Methods ◽  
Food Ingredient ◽  
Extraction Solvent ◽  
Pigmented Rice ◽  
Dpph Scavenging Activity ◽  
Extraction Solvents ◽  
Dpph Scavenging

Rice bran (RB) corresponds to the outer layers of whole grain rice and contains several phenolic compounds (PCs) that make it an interesting functional food ingredient. PC richness is enhanced in pigmented RB varieties and requires effective ways of extraction of these compounds. Therefore, we investigated conventional and deep eutectic solvents (DES) extraction methods to recover a wide array of PCs from red and black RB. The RB were extracted with ethanol/water (60:40, v/v) and two DES (choline chloride/1.2-propanediol/water, 1:1:1 and choline chloride/lactic acid, 1:10, mole ratios), based on Generally Recognized as Safe (GRAS) components. Besides the quantification of the most typical phenolic acids of cereals, nontargeted metabolomic approaches were applied to PCs profiling in the extracts. Globally, metabolomics revealed 89 PCs belonging to flavonoids (52%), phenolic acids (33%), other polyphenols (8%), lignans (6%) and stilbenes (1%) classes. All extracts, whatever the solvents, were highly concentrated in the main phenolic acids found in cereals (37–66 mg/100 g in black RB extracts vs. 6–20 mg/100 g in red RB extracts). However, the PC profile was highly dependent on the extraction solvent and specific PCs were extracted using the acidic DES. The PC-enriched DES extracts demonstrated interesting DPPH scavenging activity, which makes them candidates for novel antioxidant formulations.

A Development Strategy of Tailor-made Natural Deep Eutectic Solvents for the Enhanced Extraction of Hydroxynaphthoquinones from Alkanna tinctoria Roots

Planta Medica ◽  
2022 ◽  
Author(s):  
Elodie Bossard ◽  
Nikolaos Tsafantakis ◽  
Nektarios Aligiannis ◽  
Nikolas Fokialakis
Keyword(s):  
Development Strategy ◽  
Extraction Efficiency ◽  
Solid Phase ◽  
Eutectic Mixture ◽  
Deep Eutectic Solvents ◽  
Molar Ratio ◽  
Green Solvents ◽  
Natural Deep Eutectic Solvents ◽  
Alkanna Tinctoria ◽  
The Impact

Natural hydroxynaphthoquinone enantiomers (HNQs) are well-described pharmaceutical and cosmeceutical agents especially present in the roots of Alkanna tinctoria (L.) Tausch, a species native to the Mediterranean region. In this work, eco-friendly natural deep eutectic solvents (NaDESs) were developed for the selective extraction of these compounds. An extensive screening was performed using more than sixty tailor-made NaDESs. The impact of the intrinsic physicochemical properties on the HNQs extraction efficiency as well as the specificity towards the different enantiomeric pairs was thoroughly investigated. As a result of a multivariate analysis and of the one factor-a-time solvent optimization, the eutectic mixture composed of levulinic acid and glucose (LeG) using a molar ratio of 5:1 (molHBA:molHBD) and 20% of water (w/w) was found as the most appropriate mixture for the highest extraction efficiency of HNQs. Further optimization of the extraction process was attained by response surface methodology, using a temperature of 45 °C, a solid-to-liquid ratio of 30 mg/mL, and an extraction time of 50 min. A maximum extraction output of 41.72 ± 1.04 mg/g was reached for HNQs, comparable to that of the commonly used organic solvents. A solid-phase extraction step was also proposed for the recovery of HNQs and for NaDESs recycling. Our results revealed NaDESs as a highly customizable class of green solvents with remarkable capabilities for the extraction of HNQs.

Characterization of non polar-polar solvent extracts from some tea plantation shade tree leaves with special reference to antioxidant and antibacterial activities

2020 ◽  
pp. 15-21
Author(s):  
Arindam Ghosh ◽  
Soumya Majumder ◽  
Sumedha Saha ◽  
Malay Bhattacharya
Keyword(s):  
Cardiac Glycosides ◽  
Polar Solvent ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Shade Trees ◽  
Crude Fibre ◽  
Shade Tree ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively.  Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water,  hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.

Relationship between big five personality model and abusive supervision

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Bibi Tahira ◽  
Naveed Saif ◽  
Muhammad Haroon ◽  
Sadaqat Ali
Keyword(s):  
Big Five ◽  
Abusive Supervision ◽  
State Universities ◽  
Big Five Personality ◽  
Big Five Model ◽  
The Impact ◽  
First Time ◽  
Supervisor Behavior ◽  
Moderating Role

The current study tries to understand the diverse nature of relationship between personality Big Five Model (PBFM) and student's perception of abusive supervision in higher education institutions of Khyber Pakhtoonkhwa Pakistan. Data was collected in dyads i.e. (supervisors were asked to rate their personality attributes while student were asked to rate the supervisor behavior) through adopted construct. For this purpose, data was collected from three government state universities and one Private Sector University. The focus was on MS/M.Phill and PhD student and their supervisors of the mentioned universities. After measuring normality and validity regression analysis was conducted to assess the impact of supervisor personality characteristics that leads to abusive supervision. Findings indicate interestingly that except agreeableness other four attributes of (PBFM) are play their role for abusive supervision. The results are novel in the nature as for the first time Neuroticism, openness to experience, extraversion and conscientiousness are held responsible for the abusive supervision. The study did not explore the demographic characteristics, and moderating role of organizational culture, justice and interpersonal deviances to understand the strength of relationship in more detail way. Keywords: Personality big five model, abusive supervision, HEIs

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