scholarly journals Multi-Mycotoxin Occurrence in Dairy Cattle Feeds from the Gauteng Province of South Africa: A Pilot Study Using UHPLC-QTOF-MS/MS

Toxins ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 294 ◽  
Author(s):  
Rumbidzai Changwa ◽  
Wilfred Abia ◽  
Titus Msagati ◽  
Hlengilizwe Nyoni ◽  
Khanyisa Ndleve ◽  
...  
Keyword(s):  
South Africa ◽  
Fumonisin B1 ◽  
Cold Climate ◽  
Natural Occurrence ◽  
Gauteng Province ◽  
Toxigenic Fungi ◽  
Livestock Sector ◽  
Regulatory Limits ◽  
Liquid Chromatography Tandem Mass ◽  
Cattle Feeds

The indispensable nature of toxigenic fungi and mycotoxins in agricultural systems is of worldwide concern, hence the need for surveillance studies to preserve public health. Thirteen dairy farms were surveyed and 40 dairy feeds of varying nature collected and analyzed for mycotoxins. Estimated levels of aflatoxins (AFs), fumonisin B1 (FB1), ochratoxin A (OTA), citrinin (CIT), zearalenone (ZEN), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), deoxynivalenol (DON), 3- and 15-acetyl-deoxynivalenol (ADONs), HT-2 toxin (HT-2), and beauvericin (BEA) were established using liquid chromatography-tandem mass spectrometry. Highest frequencies (40/40) were found for AFG2 (range: <LOQ—116.1 ppb), α-ZEL (range: 0.98–13.24 ppb), and β-ZEL (range: 0.73–4.71 ppb), followed by AFB2 at 37/40 (range: <LOQ—23.88 ppb), BEA at 36/40 (range: <LOQ—55.99 ppb), HT-2 at 35/40 (range: <LOQ—312.95 ppb), and FB1 at 34/40 (range: <LOQ—1389.62 ppb). Apart from samples exceeding regulatory limits for total AFs in dairy feeds due to the high amounts of AFG2 and AFB2, levels of other mycotoxins were regarded as safe for dairy production in South Africa. This is the first-time the natural occurrence of the cold climate HT-2 in South African feeds was documented. Persistent co-occurrence of multiple mycotoxins across samples, however, may elicit synergistic and/or additive effects in hosts, hence raising concerns about their impacts and how such interactions may affect the dairy livestock sector.

Occurrence and concentrations of residues of tetracyclines, polyether ionophores and anthelmintics in livers of chickens sold in the informal market in Gauteng province, South Africa

2020 ◽  
Author(s):  
Abiodun Adesiyun ◽  
FOLORUNSO O. FASINA ◽  
OVOKEROYE A. ABAFE ◽  
MALESEDI MOKGOATLHENG-MAMOGOBO ◽  
OLUWATOLA ADIGUN ◽  
...  
Keyword(s):  
South Africa ◽  
High Performance ◽  
Maximum Residue Limit ◽  
Regulatory Enforcement ◽  
Gauteng Province ◽  
Informal Market ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
The Mean ◽  
Tetracycline Group

The occurrence, concentrations and variables associated with tetracycline, polyether ionophore and anthelmintic residues in the livers of chickens sold in the informal market in South Africa were determined. An ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) was used to simultaneously analyze for four tetracyclines, five polyether ionophores and six anthelmintic residues. The study determined the presence  of residues  in liver samples at both the limit of quantifications (LoQ) and concentrations over the maximum residue limit (MRLs), i.e. non-compliant. Doxycycline (tetracycline group) was detected in 24.5% (24/98) of chicken livers and 15.3% (15/98) were non-compliant. The mean±SD concentration of 919.04±1081.30 (LoQ) and 1410.57±108.89 ppb (MRL) were obtained. Maduramycin was detected in 27.6% (27/98) of chicken livers and 19.4% (19/98) were non-compliant. The mean±SD for LoQ was 117.96±84.56 and MRL was 153.21±76.29 ppb. The concentrations of residues of doxycycline and maduramycin in chicken livers varied significantly across townships. Lasalocid was quantified in 31.6% (31/98) of the samples, of which 5.1% (5/98) contained concentrations above the MRL. The  mean±SD concentrations of Lasalocid was 62.90±170.84 for samples in which  Lasalocid was quantified and 310.16±356.68 ppb for non-compliant samples. The frequencies of chicken livers that contained detectable concentrations of the three anthelmintic residues were 3.1% (3/98), 1.0% (1/48) and 2.0% (2/98) for praziquantel, closantel and rafoxanide, respectively. The presence of three classes of veterinary drugs residues in chicken liver poses food safety implications to consumers and indicates a need for enhanced regulatory enforcement in controlling these drugs in South Africa.

scholarly journals Investigation of the Metabolic Profile and Toxigenic Variability of Fungal Species Occurring in Fermented Foods and Beverage from Nigeria and South Africa Using UPLC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 85 ◽  
Author(s):  
Ifeoluwa Adekoya ◽  
Patrick Njobeh ◽  
Adewale Obadina ◽  
Sofie Landschoot ◽  
Kris Audenaert ◽  
...  
Keyword(s):  
South Africa ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Cyclopiazonic Acid ◽  
Positive Association ◽  
Ultra Performance Liquid Chromatography ◽  
Fungal Species ◽  
Fermented Foods ◽  
Liquid Chromatography Tandem Mass ◽  
Animal Safety

Fungal species recovered from fermented foods and beverage from Nigeria and South Africa were studied to establish their toxigenic potential in producing an array of secondary metabolites including mycotoxins (n = 49) that could compromise human and animal safety. In total, 385 fungal isolates were grown on solidified yeast extract sucrose agar. Their metabolites were extracted and analyzed via ultra-performance liquid chromatography tandem mass spectrometry. To examine the grouping of isolates and co-occurrence of metabolites, hierarchal clustering and pairwise association analysis was performed. Of the 385 fungal strains tested, over 41% were toxigenic producing different mycotoxins. A. flavus and A. parasiticus strains were the principal producers of aflatoxin B1 (27–7406 µg/kg). Aflatoxin B1 and cyclopiazonic acid had a positive association. Ochratoxin A was produced by 67% of the A. niger strains in the range of 28–1302 µg/kg. The sterigmatocystin producers found were A. versicolor (n = 12), A. amstelodami (n = 4), and A. sydowii (n = 6). Apart from P. chrysogenum, none of the Penicillium spp. produced roquefortine C. Amongst the Fusarium strains tested, F. verticillioides produced fumonisin B1 (range: 77–218 µg/kg) meanwhile low levels of deoxynivalenol were observed. The production of multiple metabolites by single fungal species was also evident.

Ascertaining the Role of Leadership on Employee Satisfaction in Market and Social Research Industries in Gauteng Province of South Africa Using Qualitative Research

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Kingstone Mutsonziwa ◽  
Philip Serumaga-Zake
Keyword(s):  
South Africa ◽  
Employee Satisfaction ◽  
Quantitative Research ◽  
Social Research ◽  
Labour Productivity ◽  
Working Environment ◽  
Gauteng Province ◽  
Career Growth ◽  
Quantitative Results ◽  
Mixed Approach

This paper is based on the study a Doctor of Business Leadership (DBL) thesis titled A Statistical Model for Employee Satisfaction in the Market and Social Research Industries in Gauteng Province. The purpose of this study was to identify the attributes that affect employee satisfaction in the Market and Social Research Industries in Gauteng Province, South Africa. In order to address the overall objective of this study, the researcher used a two-tiered (mixed) approach in which both qualitative and quantitative research methodologies were used to complement and enrich the results. This paper is only based on the qualitative component of the study on leadership aspects based on six leaders (two from Social research and four from Market research) that were interviewed. The leaders were selected based on their knowledge of the industry and the expertise they have. Participation in the survey was voluntary. This paper illustrates the power of the qualitative techniques to uncover or unmask the leadership aspects in the Market and Social Research Industries and also gives the human touch to the quantitative results. It was found that leadership and management within the Market and Social Research Industries in Gauteng Province must ensure that they are accommodative in terms of mentoring their subordinates. The industry is driven by quality driven processes and strong leadership. More importantly, issues of a good working environment, remuneration, career growth, and recognition must always be addressed in order to increase employee satisfaction, reduce staff turnover, and attempt to optimize labour productivity. The qualitative findings also help a deeper understanding of leadership within the industry.

The Vergenoeg: Gauteng Province, South Africa Fluorite Mine

2008 ◽  
Vol 83 (5) ◽  
pp. 410-421 ◽  
Author(s):  
Bruce Cairncross ◽  
Wolfgang Windisch ◽  
Henk Smit ◽  
Allan Fraser ◽  
Jens Gutzmer
Keyword(s):  
South Africa ◽  
Gauteng Province

scholarly journals Co-Occurrence of 35 Mycotoxins: A Seven-Year Survey of Corn Grain and Corn Silage in the United States

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 516
Author(s):  
Alexandra C. Weaver ◽  
Daniel M. Weaver ◽  
Nicholas Adams ◽  
Alexandros Yiannikouris
Keyword(s):  
United States ◽  
Animal Health ◽  
Fusaric Acid ◽  
Fumonisin B1 ◽  
The United States ◽  
Ultra Performance Liquid Chromatography ◽  
Liquid Chromatography Tandem Mass ◽  
And Performance ◽  
The Impact ◽  
Corn Grain

Mycotoxins contaminate crops worldwide and play a role in animal health and performance. Multiple mycotoxins may co-occur which may increase the impact on the animal. To assess the multiple mycotoxin profile of corn (Zea mays), we conducted a 7-year survey of new crop corn grain and silage in the United States. A total of 711 grain and 1117 silage samples were collected between 2013 and 2019 and analyzed for the simultaneous presence of 35 mycotoxins using ultra-performance liquid chromatography–tandem mass spectrometry. The measured mean number of mycotoxins per sample were 4.8 (grain) and 5.2 (silage), ranging from 0 to 13. Fusaric acid (FA) was most frequently detected in 78.1 and 93.8% of grains and silages, respectively, followed by deoxynivalenol (DON) in 75.7 and 88.2% of samples. Fumonisin B1 (FB1), fumonisin B2 and 15-acetyl-deoxynivalenol (15ADON) followed. The greatest (p < 0.05) co-occurrence was between FA and DON in 59.1% of grains and 82.7% of silages, followed by FA with FB1, DON with 15ADON, and FA with 15ADON. Although many samples had lower mycotoxin concentrations, 1.6% (grain) and 7.9% (silage) of tested samples had DON ≥ 5000 µg/kg. Fumonisins were detected ≥ 10,000 µg/kg in 9.6 and 3.9% of grain and silage samples, respectively. Concentrations in grain varied by year for eight mycotoxin groups (p < 0.05), while all 10 groups showed yearly variations in silage. Our survey suggest that multiple mycotoxins frequently co-occur in corn grain and silage in the Unites States, and some of the more prevalent mycotoxins are those that may not be routinely analyzed (i.e., FA and 15ADON). Assessment of multiple mycotoxins should be considered when developing management programs.

scholarly journals Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglets

2013 ◽  
Vol 6 (3) ◽  
pp. 299-308 ◽  
Author(s):  
S. Gambacorta ◽  
H. Solfrizzo ◽  
A. Visconti ◽  
S. Powers ◽  
A.M. Cossalter ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Dose Response ◽  
Fumonisin B1 ◽  
Correlation Coefficients ◽  
Urinary Biomarkers ◽  
Post Dose ◽  
Toxigenic Fungi ◽  
Biomarkers Of Exposure ◽  
Commission Recommendation ◽  
Aflatoxin B

The multi-biomarker approach was used to validate urinary biomarkers in piglets administered boluses contaminated with mixtures of deoxynivalenol (DON), aflatoxin B1 (AFB1), fumonisin B1 (FB1), zearalenone (ZEA) and ochratoxin A (OTA) at different concentrations. Boluses contaminated with mycotoxins were prepared by slurrying and freezedrying feed material fortified with culture extracts of selected toxigenic fungi. Piglets were individually placed in metabolic cages to collect urine before gavage and 24 h post dose. Urine samples were hydrolysed with β-glucuronidase and analysed by a multi-biomarker LC-MS/MS method developed and validated to identify and measure biomarkers of FB1, OTA, DON, ZEA and AFB1. Urinary levels of FB1, OTA, DON + de-epoxy-deoxynivalenol, ZEA + alphazearalenol and aflatoxin M1 were selected as biomarkers of FB1, OTA, DON, ZEA and AFB1, respectively. Mean percentages of dietary mycotoxins excreted as biomarkers in 24 h post dose urine were 36.8% for ZEA, 28.5% for DON, 2.6% FB1, 2.6% for OTA and 2.5% for AFB1. A good correlation was observed between the amount of mycotoxins ingested and the amount of relevant biomarkers excreted in 24 h post dose urine. Linear dose-response correlation coefficients ranged between 0.68 and 0.78 for the tested couples of mycotoxin/biomarker. The good sensitivity of the LC-MS/MS method and the good dose-response correlations observed in this study permitted to validate the selected mycotoxin biomarkers in piglets at dietary levels close to the maximum permitted levels reported in Commission Directive 2003/100/EC for AFB1 and the guidance values reported in Commission Recommendation 2006/576/EC for DON, ZEA, OTA and FB1.

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