Comparative In Vitro Assessment of a Range of Commercial Feed Additives with Multiple Mycotoxin Binding Claims

Contamination of animal feed with multiple mycotoxins is an ongoing and growing issue, as over 60% of cereal crops worldwide have been shown to be contaminated with mycotoxins. The present study was carried out to assess the efficacy of commercial feed additives sold with multi-mycotoxin binding claims. Ten feed additives were obtained and categorised into three groups based on their main composition. Their capacity to simultaneously adsorb deoxynivalenol (DON), zearalenone (ZEN), fumonisin B1 (FB1), ochratoxin A (OTA), aflatoxin B1 (AFB1) and T-2 toxin was assessed and compared using an in vitro model designed to simulate the gastrointestinal tract of a monogastric animal. Results showed that only one product (a modified yeast cell wall) effectively adsorbed more than 50% of DON, ZEN, FB1, OTA, T-2 and AFB1, in the following order: AFB1 > ZEN > T-2 > DON > OTA > FB1. The remaining products were able to moderately bind AFB1 (44–58%) but had less, or in some cases, no effect on ZEN, FB1, OTA and T-2 binding (<35%). It is important for companies producing mycotoxin binders that their products undergo rigorous trials under the conditions which best mimic the environment that they must be active in. Claims on the binding efficiency should only be made when such data has been generated.

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In vitro assessment of the starch digestibility of western Canadian wheat market classes and cultivars

Canadian Journal of Animal Science ◽

10.1139/cjas-2017-0099 ◽

2018 ◽

Vol 98 (3) ◽

pp. 463-476 ◽

Cited By ~ 4

Author(s):

Namalika D. Karunaratne ◽

Dawn A. Abbott ◽

Ravindra N. Chibbar ◽

Pierre J. Hucl ◽

Curtis J. Pozniak ◽

...

Keyword(s):

Crude Protein ◽

In Vitro Model ◽

Starch Digestibility ◽

Wheat Market ◽

In Vitro Assessment ◽

Market Class ◽

Small Intestinal ◽

Vitro Model ◽

Total Starch

The objective of the study was to measure the effect of wheat market class and cultivar on starch digestibility using an in vitro model that mimics the chicken digestive tract and relate it to grain characteristics. The study evaluated 18 wheat cultivars from eight western Canadian wheat classes and, each cultivar was replicated four times. Samples were subjected to gastric and small intestine (SI) digestion phases and each sample was assayed in triplicate; glucose release was measured in SI phase. Starch granule distribution, amylose, total starch, crude protein (CP), ash, and non-starch polysaccharides (NSP) were analyzed in all wheat samples. Small intestinal phase times of 15, 60, and 120 min were chosen to approximate digestion in the terminal duodenum, jejunum, and ileum. Starch digestibility of wheat classes ranged as follows: 15 min — 33.1% to 49.1%, 60 min — 80.2% to 93.3%, and 120 min — 92.4% to 97.6%. Starch digestibility positively correlated with CP, ash, NSP, and proportion of large granules, whereas it negatively correlated with total starch, and proportion of small and medium granules. In conclusion, market class and cultivar of western Canadian wheat affects both rate and extent of starch digestibility and it is related to various grain characteristics.

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Absorption of Fumonisin B1 and aminopentol on an in vitro model of intestinal epithelium; the role of P-glycoprotein

Toxicon ◽

10.1016/j.toxicon.2004.10.015 ◽

2005 ◽

Vol 45 (3) ◽

pp. 285-291 ◽

Cited By ~ 24

Author(s):

I. De Angelis ◽

G. Friggè ◽

F. Raimondi ◽

A. Stammati ◽

F. Zucco ◽

...

Keyword(s):

Intestinal Epithelium ◽

In Vitro Model ◽

Fumonisin B1 ◽

P Glycoprotein ◽

Vitro Model

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An in Vitro Assessment of a Monitor for Continuous Inline Measurement of PO2, PCO2 and pH during Cardiopulmonary Bypass

Perfusion ◽

10.1177/026765918700200209 ◽

1987 ◽

Vol 2 (2) ◽

pp. 139-147 ◽

Cited By ~ 10

Author(s):

R Peter Alston ◽

Arthur Trew

Keyword(s):

Cardiopulmonary Bypass ◽

In Vitro Model ◽

Blood Gas Analysis ◽

Systematic Errors ◽

Gas Analysis ◽

Blood Gas ◽

In Vitro Assessment ◽

Vitro Model ◽

Inline Measurement

The Gas STAT is a monitor which continuously measures PO2. PCO2 and pH inline during cardiopulmonary bypass. Using an in vitro model, the monitor's accuracy was compared to standard blood gas analysis over a range of PO2 (2·0–66·7 kPa), PCO2 (2·7–12·0 kPa) and pH (7–8). Its stability as affected by time, temperature, flow and haematocrit and the presence of halothane, enflurane, isoflurane and sodium nitroprusside was examined. Good correlations were found between the monitor and standard blood gas analysis for PO2 (r = 0·999, P < 0·001) and PCO2 (r = 0·996, P < 0·001). However, large and unpredictable systematic errors occurred. It was stable under all conditions examined, except that in one sensor there was a statistically significant decline in PCO2 measurement with time (P < 0·005) and the PCO2 readings were affected by temperature (P < 0·021). The monitor provides useful information for blood gas management during cardiopulmonary bypass, but should not be used without recourse to standard blood gas analysis.

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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