scholarly journals Infectious Bronchitis Virus Evolution, Diagnosis and Control

2020 ◽  
Vol 7 (2) ◽  
pp. 79 ◽  
Author(s):  
Matteo Legnardi ◽  
Claudia Maria Tucciarone ◽  
Giovanni Franzo ◽  
Mattia Cecchinato
Keyword(s):  
Infectious Bronchitis Virus ◽  
Immune Escape ◽  
Control Strategies ◽  
Rapid Evolution ◽  
Emerging Diseases ◽  
Economic Losses ◽  
Recombination Rates ◽  
Infectious Bronchitis ◽  
Advantages And Disadvantages ◽  
And Control

RNA viruses are characterized by high mutation and recombination rates, which allow a rapid adaptation to new environments. Most of the emerging diseases and host jumps are therefore sustained by these viruses. Rapid evolution may also hinder the understanding of molecular epidemiology, affect the sensitivity of diagnostic assays, limit the vaccine efficacy and favor episodes of immune escape, thus significantly complicating the control of even well-known pathogens. The history of infectious bronchitis virus (IBV) fits well with the above-mentioned scenario. Despite being known since the 1930s, it still represents one of the main causes of disease and economic losses for the poultry industry. A plethora of strategies have been developed and applied over time, with variable success, to limit its impact. However, they have rarely been evaluated objectively and on an adequate scale. Therefore, the actual advantages and disadvantages of IBV detection and control strategies, as well as their implementation, still largely depend on individual sensibility. The present manuscript aims to review the main features of IBV biology and evolution, focusing on their relevance and potential applications in terms of diagnosis and control.

scholarly journals Design and Characterization of a DNA Vaccine Based on Spike with Consensus Nucleotide Sequence against Infectious Bronchitis Virus

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Lei Zuo ◽  
Wenjun Yan ◽  
Zhou Song ◽  
Hao Li ◽  
Xin Xie ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dna Vaccine ◽  
Infectious Bronchitis Virus ◽  
Neutralizing Antibody ◽  
Economic Losses ◽  
Post Translational Modification ◽  
Virus Shedding ◽  
Infectious Bronchitis ◽  
Specific Pathogen ◽  
Cellular Immune

Avian coronavirus infectious bronchitis virus (IBV) causes severe economic losses in the poultry industry, but its control is hampered by the continuous emergence of new genotypes and the lack of cross-protection among different IBV genotypes. We designed a new immunogen based on a spike with the consensus nucleotide sequence (S_con) that may overcome the extraordinary genetic diversity of IBV. S_con was cloned into a pVAX1 vector to form a new IBV DNA vaccine, pV-S_con. pV-S_con could be correctly expressed in HD11 cells with corresponding post-translational modification, and induced a neutralizing antibody response to the Vero-cell-adapted IBV strain Beaudette (p65) in mice. To further evaluate its immunogenicity, specific-pathogen-free (SPF) chickens were immunized with the pV-S_con plasmid and compared with the control pVAX1 vector and the H120 vaccine. Detection of IBV-specific antibodies and cell cytokines (IL-4 and IFN-γ) indicated that vaccination with pV-S_con efficiently induced both humoral and cellular immune responses. After challenge with the heterologous strain M41, virus shedding and virus loading in tissues was significantly reduced both by pV-S_con and its homologous vaccine H120. Thus, pV-S_con is a promising vaccine candidate for IBV, and the consensus approach is an appealing method for vaccine design in viruses with high variability.

scholarly journals Cocirculation of Four Infectious Bronchitis Virus Lineages in Broiler Chickens in the Eastern Region of Saudi Arabia from 2012 to 2014

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Abdullah I. A. Al-Mubarak ◽  
Anwar A. G. Al-Kubati
Keyword(s):  
Saudi Arabia ◽  
Middle East ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Direct Sequencing ◽  
Hypervariable Region ◽  
Economic Losses ◽  
Eastern Region ◽  
Infectious Bronchitis ◽  
First Time

Avian infectious bronchitis virus (IBV) is an evolving and dynamic virus that causes major economic losses for the poultry industry worldwide. Continuous evolution and emergence of new variants of this virus are the major challenges for controlling the disease with routine vaccination. Successful vaccination usually requires the use of a homologous vaccine, which in turn necessitates continuous investigation of the circulating strains. Herein, we performed a reverse transcriptase-polymerase chain reaction- (RT-PCR-) based investigation in broiler chicken flocks of the Eastern Region of Saudi Arabia. IBV was detected in 36.5% of the tested flocks (42 out of 115) from January 2012 to March 2014. Direct sequencing of hypervariable region-3 (HVR-3) of the Spike (S)-1 gene was performed, followed by phylogenetic analysis to determine the circulating IBV genotypes. Four lineages appear to coexist in this region, including the GI-13 or 4/91 IBV (31%), GI-16 or CK/CH/LDL/97I IBV (28.6%), GI-1 or Mass IBV (19%), and GI-23 or Middle East IBV (21.4%). The latter lineage include two subgroups: IS/720/99 IBV (16.7%) and IS/Variant2/98 IBV (4.7%). Some of the detections made in the 4/91 and Mass lineages are expected to belong to the vaccine strains. Lineages without a homologous vaccine in use (CK/CH/LDL/97I and Middle East) represent 50% of the isolates recovered in this study. Based on identity with the vaccine sequences, field observations, and frequent detection, these two lineages appear to be out of coverage of the IBV vaccines used in Saudi Arabia. This is the first time to identify Middle East lineage (IS/720/99 IBV and IS/Variant2/98 IBV) in the Eastern Region of Saudi Arabia.

scholarly journals Greater Protection against Virulent Infectious Bronchitis Virus Challenge Conferred by Recombinant Baculovirus Co-expressing S1 and N Proteins

2018 ◽  
Author(s):  
Yuan Yuan ◽  
Zhi-Peng Zhang ◽  
Yi-Ning He ◽  
Wen-Sheng Fan ◽  
Zhi-Hua Dong ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Infectious Bronchitis Virus ◽  
Subunit Vaccine ◽  
Recombinant Baculovirus ◽  
Recombinant Baculoviruses ◽  
Economic Losses ◽  
Subunit Vaccines ◽  
Infectious Bronchitis ◽  
Antibody Levels ◽  
N Proteins

Avian infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis, which causes considerable economic losses to the poultry industry worldwide. It is imperative to develop safe and efficient candidate vaccines to control IBV infection. In the current study, recombinant baculoviruses co-expressing S1 and N proteins, mono-expressing S1 or N proteins alone of IBV were constructed and prepared into subunit vaccines rHBM-S1-N, rHBM-S1 and rHBM-N. The levels of immune protection of these subunit vaccines were evaluated by inoculating specific pathogen-free (SPF) chickens at 14 days of age, boosting with the same dose 14 days later, and following challenge with a virulent GX-YL5 strain of IBV 14 days post-booster (dpb). The commercial vaccine strain H120 was used as a control. The IBV-specific antibody levels as well as the percentages of CD4+ and CD8+ T lymphocytes were detected within 28 days post-vaccination (dpv). The morbidity, mortality, and re-isolation of virus from the tracheas and kidneys of challenged birds were evaluated at 5 days post-challenge (dpc). The results showed that the IBV-specific antibody levels and the percentages of CD4+ and CD8+ T lymphocyte in rHBM-S1-N group were higher than those of rHBM-S1 and rHBM-N groups, especially the cellular immunity response. At 5 dpc, the mortality, morbidity and virus re-isolation rate of rHBM-S1-N were slightly higher than those of H120 group, but were lower than those of rHBM-S1 group and rHBM-N group. The present study demonstrated that the protection of recombinant baculovirus co-expressing S1 and N proteins was better than that of recombinant baculoviruses mono-expressing S1 or N protein alone. Thus, the recombinant baculovirus co-expressing S1 and N proteins could serve as a potential IBV vaccine and this demonstrates that the bivalent subunit vaccine including the S1 and N proteins might be a strategy for the development of an IBV subunit vaccine.

Molecular detection and epidemiology of chicken infectious anaemia virus in India

2013 ◽  
Vol 3 (4) ◽  
pp. 145-151
Author(s):  
M. Y. Wani ◽  
K. Dhama ◽  
R. Barathidasan ◽  
V. Gowthaman ◽  
R. Tiwari ◽  
...  
Keyword(s):  
Prevention And Control ◽  
Molecular Detection ◽  
Control Strategies ◽  
Age Groups ◽  
Epidemiological Studies ◽  
Control Measures ◽  
Economic Losses ◽  
Field Samples ◽  
And Control ◽  
Young Chicks

Chicken infectious anemia (CIA) is an economically important disease affecting poultry, especially young chicks, characterized by poor weight gain, anemia, immunosuppression and production losses. Recent reports indicate that the virus is emerging and re-emerging worldwide.  Also, scarce reports regarding the molecular detection and epidemiology of virus from India are available. The aim of this study was to determine the prevalence of the virus in the commercial poultry farms of the country employing polymerase chain reaction (PCR). Out of the suspected 424 field samples tested from 12 different states of the country during the last five years (2007-2012), 311 (73.3%) samples were found positive for CIAV-DNA indicating the wide prevalence of the virus in India. The age wise prevalence of the virus indicated high prevalence in young chicks of up to three weeks (80.3%) of age compared to 3-7 (66.6%) and 7-12 (25%) week age groups. Being an immunosuppressive emerging pathogen circulating among the poultry flocks of the country, suitable prevention and control measures need to be adopted timely. This is a first elaborative report on the molecular epidemiology of CIAV in poultry flocks of the country. Extensive epidemiological studies are suggested to know the magnitude of the virus affecting poultry industry of the country, and design and formulate effective disease prevention and control strategies to reduce economic losses being caused by emerging virus.

scholarly journals Infectious Bronchitis Virus in Egypt: Genetic Diversity and Vaccination Strategies

2020 ◽  
Vol 7 (4) ◽  
pp. 204
Author(s):  
Hassanein H. Abozeid ◽  
Mahmoud M. Naguib
Keyword(s):  
Infectious Bronchitis Virus ◽  
Negative Impact ◽  
Current Knowledge ◽  
Economic Losses ◽  
Poultry Production ◽  
Infectious Bronchitis ◽  
Vaccination Strategies ◽  
Avian Pathogen ◽  
Surveillance Programs ◽  
Insight Into

Infectious bronchitis virus (IBV) is a highly evolving avian pathogen that has increasingly imposed a negative impact on poultry industry worldwide. In the last 20 years, IBV has been continuously circulating among chicken flocks in Egypt causing huge economic losses to poultry production. Multiple IBV genotypes, namely, GI-1, GI-13, GI-16, and GI-23 have been reported in Egypt possessing different genetic and pathogenic features. Different vaccine programs are being used to control the spread of the disease in Egypt. However, the virus continues to spread and evolve where multiple IBV variants and several recombination evidence have been described. In this review, we highlight the current knowledge concerning IBV circulation, genesis, and vaccination strategies in Egypt. In addition, we analyze representative Egyptian IBV strains from an evolutionary perspective based on available data of their S1 gene. We also provide insight into the importance of surveillance programs and share our perspectives for better control of IBV circulating in Egypt.

scholarly journals Evolutionary Analysis of Infectious Bronchitis Virus Reveals Marked Genetic Diversity and Recombination Events

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 605 ◽  
Author(s):  
Mohammed A. Rohaim ◽  
Rania F. El Naggar ◽  
Mohammed A. Abdelsabour ◽  
Mahmoud H. A. Mohamed ◽  
Ibrahim M. El-Sabagh ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Genetic Characterization ◽  
Economic Losses ◽  
N Gene ◽  
Evolutionary Analysis ◽  
Vaccination Programs ◽  
Viral Attachment ◽  
Recombinant Viruses ◽  
Infectious Bronchitis ◽  
Pcr Targeting

In the last 5 years, frequent outbreaks of infectious bronchitis virus (IBV) are observed in both broiler and layer chicken flocks in the Kingdom of Saudi Arabia (KSA) in spite of extensive usage of vaccines. The IBV is a widespread avian coronavirus affecting both vaccinated and unvaccinated chicken flocks and is attributed to significant economic losses, around the globe. In the present study, 58 (n = 58) samples were collected from four different commercial poultry flocks from 8 KSA districts during 2019. A total of nine positive isolates (9/58; 15.5%), based on real-time reverse transcriptase PCR targeting nucleocapsid (N) gene, were used for further genetic characterization and evolutionary analysis. Genetic characterization of the partial spike (S1) gene revealed the clustering of the reported isolates into three different genotypes, whereas four additional isolates were grouped within 4/91 genotype, two isolates within IS/885 genotype, one isolate was closely related to IS/1494/06, and two isolates were grouped within classic serotype (vaccine-like strains). Phylodynamic revealed clustering of four isolated viruses within GI-13 lineage, three isolates within GI-23 lineage, and two isolates within GI-1 lineage. Results indicate that there are high evolutionary distances between the newly identified IBV strains in this study and the commercially used vaccines (GI-1), suggesting that IBV strains circulating in the KSA are under constant evolutionary pressures. Selective pressure biostatistics analyses consistently demonstrate the presence of a higher positive score which highlights the role of natural selection, a mechanism of virus evolution on sites located on the protein surface, within or nearby domains involved in viral attachment or related functions. Recombination analysis revealed emergence of two isolates through recombination events resulting in new recombinant viruses. Taken together, these finding demonstrate the genetic and evolutionary insights into the currently circulating IBV genotypes in KSA, which could help to better understand the origin, spread, and evolution of infectious bronchitis viruses, and to ascertain the importance of disease monitoring as well as re-evaluation for the currently used vaccines and vaccination programs.

scholarly journals Analysis of synonymous codon usage in spike protein gene of infectious bronchitis virus

2015 ◽  
Vol 61 (12) ◽  
pp. 983-989 ◽  
Author(s):  
Aditi Makhija ◽  
Sachin Kumar
Keyword(s):  
Codon Usage ◽  
Infectious Bronchitis Virus ◽  
Codon Usage Bias ◽  
Synonymous Codon ◽  
Synonymous Codon Usage ◽  
Spike Protein ◽  
Economic Losses ◽  
Infectious Bronchitis ◽  
S Gene ◽  
S Genes

Infectious bronchitis virus (IBV) is responsible for causing respiratory, renal, and urogenital diseases in poultry. IBV infection in poultry leads to high mortality rates in affected flocks and to severe economic losses due to a drop in egg production and a reduced gain in live weight of the broiler birds. IBV-encoded spike protein (S) is the major protective immunogen for the host. Although the functions of the S protein have been well studied, the factors shaping synonymous codon usage bias and nucleotide composition in the S gene have not been reported yet. In the present study, we analyzed the relative synonymous codon usage and effective number of codons (Nc) using the 53 IBV S genes. The major trend in codon usage variation was studied using correspondence analysis. The plot of Nc values against GC3 as well as the correlation between base composition and codon usage bias suggest that mutational pressure rather than natural selection is the main factor that determines the codon usage bias in the S gene. Interestingly, no association of aromaticity, degree of hydrophobicity, and aliphatic index was observed with the codon usage variation in IBV S genes. The study represents a comprehensive analysis of IBV S gene codon usage patterns and provides a basic understanding of the codon usage bias.

scholarly journals Infectious bronchitis virus variants: a review of the history, current situation and control measures

2011 ◽  
Vol 40 (3) ◽  
pp. 223-235 ◽  
Author(s):  
J. J. (Sjaak) de Wit ◽  
Jane K. A. Cook ◽  
Harold M. J. F. van der Heijden
Keyword(s):  
Infectious Bronchitis Virus ◽  
Current Situation ◽  
Control Measures ◽  
Infectious Bronchitis ◽  
And Control
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