scholarly journals Serotonin and Tryptophan Serum Concentrations in Shelter Dogs Showing Different Behavioural Responses to a Potentially Stressful Procedure

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Giacomo Riggio ◽  
Chiara Mariti ◽  
Valeria Sergi ◽  
Silvana Diverio ◽  
Angelo Gazzano

In mammals, serotonin (5-HT) levels depend on the availability of tryptophan (TRP). Low 5-HT concentrations have been linked to behavioural disorders in dogs. This study aimed at investigating possible differences in dogs’ serum TRP and 5-HT concentrations according to their behavioural response to a potentially stressful procedure. Thirty-nine physically healthy shelter dogs, 15 females and 24 males, mean age = 5.6 years, were categorized by a certified veterinary behaviourist according to their behavioural response to medical examination and blood collection, in: relaxation, stress signals, tension without growling, tension with growling, escape attempts, and aggression attempts. Extraction and quantification of 5-HT and TRP were performed using a HLPC method. Data were statistically analysed, applying Chi-square and Spearman tests. Results showed no significant difference in TRP (χ2 = 2.084, p = 0.555) nor 5-HT (χ2 = 0.972, p = 0.808) serum concentrations among different categories of dogs; however, some categories were underrepresented (relaxation = 20.5%, stress signals = 30.8%, tension without growling = 43.6%, tension with growling = 5.1%, escape attempts = 0%, aggression attempts = 0%). No correlation between serum TRP and 5-HT concentrations was found (ρ = 0.086, p = 0.602). Serum 5-HT levels do not seem to be associated with dogs’ behavioural response to a stressful situation nor with serum TRP concentrations. The relationship between serum TRP and 5-HT concentrations and behaviour needs further research.

Author(s):  
Ma. Dulce C. Guillena

Gonado-somatic index and fecundity are tools for measuring the sexual maturity and ability of animals to reproduce.  This study investigates the reproduction of Trichiurus lepturus. Specifically, this aimed to determine the sex ratio, the GSI, the relationship between fecundity and total length, fecundity and total weight, fecundity and ovary weight. The Descriptive Method of research was used.  Percentage and chi-square was utilized in determining the percentage of occurrence and sex ratio respectively.   Pearson r Product Moment Coefficient of Correlation was used to determine the relationships of the parameters. The study revealed that females outnumbered males and the sex ratio for different month showed significant difference.  Spawning season was observed to occur in November and December as revealed in its GSI values and it synchronized with the full and new moon phases.  Fecundity is positively correlated with body weight, body size, and ovary weight where ovary weight is observed to be the best index for fecundity.  The results of this study could be used further for formal stock assessment of cutlassfish fishery.


2017 ◽  
Vol 29 (1) ◽  
pp. 119 ◽  
Author(s):  
A. Swelum ◽  
A. Moumen ◽  
A. Alowaimer

This study was carried out using 80 multiparous Awassi ewes during breeding season to compare the effects of controlled internal drug release (CIDR) withdrawal time on ewe fertility. Ewes were equally and randomly allotted into 4 groups (n = 20/group). Ewes had a CIDR inserted for 3, 6, 9, or 12 days with intramuscular administration of 300 IU of equine chorionic gonadotropin (eCG) at withdrawal time. Oestrus was detected using vasectomized ram starting 12 h after CIDR withdrawal and repeated every 12 h up to 84 h. Blood samples were collected from all groups at the time of CIDR withdrawal for measuring of oestradiol (E2) and progesterone (P4) serum concentrations using commercial ELISA kits and micro-titrimetric plates. Timed insemination was performed 48 h post CIDR withdrawal in all groups. Pregnancy was diagnosed by ultrasonography at day 23 post-insemination and confirmed at day 35. Comparisons among groups were evaluated using Chi Square (χ2) test in all measured parameters except hormones levels, which analysed by repeated-measures analysis of variance (ANOVA), using SAS (SAS Institute Inc., Cary, NC, USA). A difference was considered significant at P < 0.05 level. The results revealed that the retention, vaginal discharge and drawstring breakage rates after CIDR removal were insignificantly differed between all groups. On the other hand, pregnancy rate was significantly (P ≤ 0.05) higher in 9-d and 6-d groups (68.4 and 60%, respectively) than the 3-d group. While, no significant difference was detected between 12d group and other groups. The heat detection rate was significantly (P ≤ 0.05) higher in 12d group (100%) than 6-d and 3-d groups (80 and 45%, respectively). While, no significant difference was detected between 12-d and 9-d groups in heat detection rate. P4 was significantly higher in the 12-d group (13.4 ± 3.06 ng mL−1) than other groups. While E2 was significantly lower in the 12-d group (1.6 ± 0.06 pg mL−1) than other groups. These results indicated that withdrawal of CIDR devices after 9 days are efficient in synchronizing oestrus in ewes and provided higher pregnancy rate.


2021 ◽  
Vol 9 (2) ◽  
pp. 008-014
Author(s):  
Rastin Hosseinzadeh Asli ◽  
Manouchehr Aghajanzadeh ◽  
Hossein Hosseinzadeh Asli ◽  
Yousha poorahmadi

Introduction: The most common cause of primary spontaneous pneumothorax (PSP) is sub pleural bleb apical rupture. Little is known about the relationship between PSP and exercise and return to exercise time. In this study, wee tend to investigate the relationship between exercise and PSP and time of return to exercise and previous activities. Method: This study was designed as a case series and the sample size included all patients diagnosed with PSP in Razi and Poursina and Aria hospitals of Rasht during 2015-2019 based on inclusion criteria. Variables were analyzed using Fisher's exact test, Chi square, Mann Whitney U and t-test (p<0.05). Results: The most common type of treatment in patients was transaxillary thoracotomy with pleurodesis with iodine (TTP) in 58.2% and tube thoracostomy and pleurodesis in 41.7%, which was not statistically significant between athletes and non-athletes (p=0.806). Athletes who underwent TTP after 4 weeks and those treated with tube thoracostomy and pleurodesis after 8-12 weeks were advised to return to their previous activity. Of athletes, 9.5% had recurrence; of non-athletes, 9.8% had recurrence. Of athletes, 4.8% did not tolerate a return to their previous activity; of non-athletes, 7.3% did not tolerate returning to their previous activity regardless of treatment, and this difference was not significant. Conclusion: Our study showed no significant difference between clinical manifestations and image findings as well as frequency of treatment and complications in both athlete and non-athlete patients. There is no increase in recurrence and intolerance at the time recommended for return to previous activity.


2015 ◽  
Vol 24 (1) ◽  
pp. 24-9 ◽  
Author(s):  
Syaiful A. Hadi ◽  
Dody P. Masri

Background: Palmaris longus (PL) tendon is often used as graft in tendon or ligament reconstruction. PL absence is a normal variation in human body. Its prevalence was different in various population. Serbian population had an absence rate of 42.4%, but in the Korean population it was only 4.0%. This study aimed to determine the prevalence of PL absence in various ethnic of Indonesian population and its relationship with gender and side of hand dominance. Methods: We conducted a cross-sectional study in 1230 people from six ethnicities in Indonesia. The absence of PL was determined by Schaeffer test and confirmed with Mishra and Thompson test. Data collection was performed by a single examiner in various cities in Indonesia. The relationship between PL absence with ethnicity, gender, and hand dominance were determined by Chi-square test. Results: Prevalence of PL absence in overall Indonesian population was 10.4% (bilateral 37.5% and unilateral 62.5%). The highest were in Batak and Madura ethnicities (15.5%), followed by Sunda (11.8%), Java (10.0%), Betawi (6.0%), and the lowest was Minang ethnicity (4.1%) (p = 0.008). Prevalence of PL absence in female and male were 10.7% 10.2%, respectively, p > 0.05).Conclusion: Compared to range of prevalence in other countries in the world, prevalence of PL absence in Indonesian population was moderate (10.4%). There was significant difference found between ethnicity, but not in gender. Surgeon should consider this data when planning for reconstruction procedures using PL graft in Indonesian population.


Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 133
Author(s):  
Cahit Kural ◽  
Serpil Oguztuzun ◽  
Gülçin Güler Şimşek ◽  
Servet Guresci ◽  
Pınar Kaygın ◽  
...  

Background and objectives: The pathophysiology of tethered cord syndrome (TCS) in children is not well elucidated. An inelastic filum terminale (FT) is the main factor underlying the stretching of the spinal cord in TCS. Our study aimed to investigate the expression of glutathione-S-transferase (GST) in children and fetal FT samples in order to understand the relationship between this enzyme expression and the development of TCS. Materials and Methods: FT samples were obtained from ten children with TCS (Group 1) and histological and immunohistochemical examinations were performed. For comparison, FT samples from fifteen normal human fetuses (Group 2) were also analyzed using the same techniques. Statistical comparison was made using a Chi-square test. Results: Positive GST-sigma expression was detected in eight (80%) of 10 samples in Group 1. The positive GST-sigma expression was less frequent in nine (60%) of 15 samples from Group 2. No statistically significant difference was detected between the two groups (p = 0.197). Conclusions: Decreased FT elasticity in TCS may be associated with increased GST expression in FT. More prospective studies are needed to clarify the mechanism of the GST–TCS relationship in children.


2019 ◽  
Vol 5 (3) ◽  
pp. 141
Author(s):  
Reny Ekawati ◽  
Supangat Supangat ◽  
Hairrudin Hairrudin

Perinatal death is an indicator of the success implementing a country's health services. Perinatal death rate in Indonesia in 2012 was 26/1000 live births. In 2017 there were 225 cases of infant mortality from 34,669 births in Jember Regency. Perinatal death is influenced by maternal, infant, health services and the living environment. The environmental conditions of the residence can be affected by cigarette smoke. The aim of this study was to determine the relationship of exposure tobacco smoke in pregnant women with perinatal death in Jember Regency and to find out the main source of exposure tobacco smoke in pregnant women in Jember Regency. Type of research is analytic observation with case control study design. The sampling technique is consecutive sampling. The study sample was mothers who had a dead baby in the perinatal period (perinatal death) who resided in the Jember regency on the birth of January-December 2017. The sample size was 30 for each group. Collected data was carried out by univariate analysis and bivariate analysis using the chi-square test and fisher exact test if the chi-square test requirements were not met. Results of univariate analysis in this study showed that 89.47% of the main sources of exposure tobacco smoke originated from home and 73.68% from husbands. Results of the chi-square test between exposure tobacco smoke with perinatal death p value 0.579 (p> 0.05). Confounding variables that had no significant difference between the case and control groups were parity (p = 0.116, p> 0.05), education (p = 0.083, p> 0.05), birth distance (p = 0.26, p> 0.05), and birth attendants (p = 0.492, p> 0.05). Based on the results of these studies it can be concluded that exposure tobacco smoke in pregnant women is not associated with perinatal death and the main source of exposure tobacco smoke in pregnant women comes from home and at most from husbands.   Keywords: exposure tobacco smoke, pregnant women, perinatal death  


Author(s):  
Islam NamazianDost

This study tried to investigate the role of interpersonal meta-discourse markers such as boosters. In order to illuminate this relation, 15 medical and 15 applied linguistics articles were selected randomly from around 50 articles. Therefore, quantitative and qualitative methods were applied to answer the research questions to identify the frequency and percentage of booster employment in their discussion sections. Moreover, to determine the supposedly meaningful differences between booster applications in the corpus, the chi-square test was used. The findings showed that there was not any significant difference between applied and medical articles in using boosters in their discussion section. Moreover, it was found that boosters such as it is clear that, definitely, certainly, really, totally, always were the most frequent ones which were used in two groups of articles namely applied and medical articles. Since meta-discourse markers, i.e., boosters play crucial roles in mediating the relationship between what writers intend to argue and their discourse communities, the results of the present study have obvious importance in increasing students’ awareness of the way they organize their writings.


2020 ◽  
Vol 25 (4) ◽  
pp. 297-303
Author(s):  
Min Ki Hong ◽  
Jinha Park ◽  
Sung Hoon Koh ◽  
Dong Chul Lee ◽  
Si Young Roh ◽  
...  

Purpose: Digital replantation has dramatically changed the fate of mutilated injuries of hand. But in case of severe crushing injury in Tamai zone I, it is a contraindication of replantation. This study aims to determine the replantation survival rate in cases with crushing injury.Methods: We retrospectively evaluated patients’ charts and photos from January 2015 to December 2019. There were 294 patients (331 fingers) with digital amputation who underwent surgery. Cases with crushing were divided into petechiae-in-skin group and red-line-sign group and compared with the group without crushing. The relationship of survival rate with factors was evaluated using chi-square analysis.Results: Of these, 199 fingers were defined as severe crushed, and 140 cases with petechiae in skin and 59 cases with red line sign. There was no statistically significant difference in survival rate compared with the group without crushing (p=0.227). Additional surgery was performed more in crushed injury. However, necrotized soft tissue was mainly on margin of amputation, not on crushed area.Conclusion: The presence of crushing did not affect survival rate in Tamai zone I replantation. However, as the degree of crushing was severe, the need for additional surgery increased. We found that the number of anastomosed arteries and veins influences the survival rate. According to our analysis, even if it’s difficult, finding and doing anastomosis as many vessels as possible can make good results.


2019 ◽  
Vol 4 (1) ◽  
pp. 22-28
Author(s):  
Rubia Caldas Umburanas ◽  
Priscila Costa Estabile ◽  
Rogério Pincela Mateus ◽  
Mara Cristina de Almeida ◽  
Roberto Ferreira Artoni

Systemic arterial hypertension (SAH) is a multifactorial clinical condition characterized by high and sustained levels of blood pressure (BP). Some studies have reported that variants of the angiotensin-converting enzyme (ace) gene increase the risk of hypertension. The aim of this study was to verify the existence of the relationship between the insertion/deletion (I/D) polymorphism in the ACE gene and its genotypic variants with BP in four distinct groups of hypertensive individuals and also to genetically and epidemiologically characterize the investigated samples. The study was formed of 112 individuals arranged into the following groups: normotensive (control); hypertensive and non-obese; hypertensive and obese; and, hypertensive and with type 2 diabetes mellitus (T2DM). Epidemiological data and peripheral blood were collected from participants for DNA extraction and amplification by PCR (polymerase chain reaction). The allele (D=0.5446; I=0.4554) and genotype (DD =0.2411, ID =0.6071; II =0.1518) frequencies showed low genetic differentiation (Fst<0.05) and were outside the Hardy-Weinberg equilibrium (p<0.05). There was no significant difference between the groups (chi-square=4.4335; p=0.6174). There was no association of the D allele with SAH, reinforcing the hypothesis that environmental interferences are prevalent in the evolution of SAH.


2017 ◽  
Vol 7 (10) ◽  
pp. 36 ◽  
Author(s):  
Nancy A. Sears ◽  
Maha Othman ◽  
Leah O'Neil ◽  
Wilma M. Hopman

Background/Objective: In January 2015, 10 of 12 registered nurse regulators in Canada began using the National Council Licensure Exam for Registered Nursing (NCLEX-RN) as the entry-to-practice examination. We examine the NCLEX-RN performance of BScN graduates from three sites of one program across the first 2 years of its use. We aim to investigate the relationship between undergraduate academic performance and NCLEX-RN performance, and to determine predictors of success and/or failure on the exam.Methods: A total of 215 graduates who wrote the 2015 or 2016 NCLEX-RN participated in the study. Course grades, final program percentage grade and GPAs, and students’ time to complete the program are examined against pass/fail performance on the NCLEX-RN. Student’s t test and Chi Square tests are used for comparative analysis. Logistic regression identified the odds ratio and associated 95% confidence interval for each one-unit increase in GPAs as a predictor of success.Results: Overall, 141 of 215 (66%) graduates passed NCLEX-RN and 74 (34%) failed, with no significant difference between the two years. Time to complete the program is significantly lower (p = .002) and graduating GPA is significantly higher (p < .001) among those who passed the NCLEX-RN compared to those who failed. With one exception, all course grades are significantly higher for students who passed, compared to those who failed. The odds of passing the NCLEX-RN increase by 10 (95% CI 4.5, 22.6) for each one-point increase in GPA. At a GPA of 4.0, zero failures on NCLEX-RN are observed.Conclusions: To our knowledge, this is the first Canadian systematic institutional based study examining the relationship between NCLEX-RN performance and academic factors. The study concludes high academic performance remains a strong predictor for NCLEX-RN success. Future, preferably multicenter larger studies, could further the understanding of the performance on this exam in Canada and support practices enhancing students’ success on the NCLEX-RN. 


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