scholarly journals Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Water ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 921 ◽  
Author(s):  
Jameson Filer ◽  
Huihuang H. Ding ◽  
Sheng Chang

Biochemical methane potential (BMP) tests are widely used for characterizing a substrate’s influence on the anaerobic digestion process. As of 2018, there continues to be a lack of standardization of units and techniques, which impacts the comparability and validity of BMP results. However, BMP methods continue to evolve, and key aspects are studied to further eliminate systematic errors. This paper aims to update these key aspects with the latest research progress both to introduce the importance of each variable to those new to BMP measurements and to show the complexity required to design an accurate BMP test.

2021 ◽  
Vol 11 (7) ◽  
pp. 3064
Author(s):  
Roberta Mota-Panizio ◽  
Manuel Jesús Hermoso-Orzáez ◽  
Luis Carmo-Calado ◽  
Gonçalo Lourinho ◽  
Paulo Sérgio Duque de Brito

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.


2013 ◽  
Vol 67 (2) ◽  
pp. 410-417 ◽  
Author(s):  
M. A. De la Rubia ◽  
V. Fernández-Cegrí ◽  
F. Raposo ◽  
R. Borja

Due to the chemical and physical structure of a lignocellulosic biomass, its anaerobic digestion (AD) is a slow and difficult process. In this paper, the results obtained from a batch biochemical methane potential (BMP) test and fed-batch mesophilic AD assays of sunflower oil cake (SuOC) are presented. Taking into account the low digestibility shown during one-stage experiments the methane yield decreased considerably after increasing the organic loading rate (OLR) from 2 to 3 g VS L−1 d−1, SuOC was subjected to a two-stage AD process (hydrolytic-acidogenic and methanogenic stages), in two separate reactors operating in series where the methanogenic stage became acidified (with >1,600 mg acetic acid L−1) at an OLR as low as 2 g VS L−1 d−1. More recently, BMP assays were carried out after mechanical, thermal, and ultrasonic pre-treatments to determine the best option on the basis of the methane yield obtained.


2012 ◽  
Vol 66 (7) ◽  
pp. 1416-1423 ◽  
Author(s):  
C. P. Pabón Pereira ◽  
G. Castañares ◽  
J. B. van Lier

A protocol was developed for determining the biochemical methane potential (BMP) of plant material using the OxiTop® system. NaOH pellets for CO2 absorption and different pretreatment methods were tested for their influence in the BMP test. The use of NaOH pellets in the headspace of the bottle negatively affected the stability of the test increasing the pH and inhibiting methanization. Sample comminution increased the biodegradability of plant samples. Our results clearly indicate the importance of test conditions during the assessment of anaerobic biodegradability of plant material, considering BMP differences as high as 44% were found. Guidelines and recommendations are given for screening plant material suitable for anaerobic digestion using the OxiTop® system.


2013 ◽  
Vol 827 ◽  
pp. 84-90 ◽  
Author(s):  
Maurizio Carlini ◽  
Sonia Castellucci ◽  
Silvia Cocchi

One of the most promising processes to exploit Solid Olive-Mill Waste (SOMW) for energy production is anaerobic digestion. An experimental study has been carried out on SOMW and inoculum, consisting of Cattle Slurry Digested (CSD) and coming from an anaerobic digestion plant. A substrate with an optimal supply ratio equal to 2:1 has been investigated in a reactor at 37°C by analysing the biogas production. The Biochemical Methane Potential (BMP) test has been carried out, monitoring pH, biogas production (amount and composition). According to the tests results, SOMWs needed to be diluted and inoculated, moreover the pH control is foundamental in order to obtain a significant biogas production. Anaerobic digestion plant of SOMW should be promoted in Mediterranean countries as an environmentally sound option for waste management and energy production, since olive mills are very widespread agro-industries in this area.


2018 ◽  
Vol 67 ◽  
pp. 02047 ◽  
Author(s):  
Reigina Sandriaty ◽  
Cindy Priadi ◽  
Septiana Kurnianingsih ◽  
Ayik Abdillah

The generation of fat, oil and grease (FOG) waste can be a nuisance hazard, but also a potential for resource recovery. FOG waste can be utilized as nutrient and energy source through anaerobic digestion which may increase methane yield but also increase presence of inhibitors. Using the biochemical methane potential method, this research is aimed to determine the effect of FOG waste in the co-digestion process of food waste (FW) to produce biogas. The research was conducted for 42 days at 37°C using FOG waste codigested with FW of 3 different volatile solid (VS) rasio which are 0.125, 0.3, and 0.5. The results showed that FOG waste combined with FW has a methane yield that may reach up to 485 ± 36.8 mL CH4/gr VS, the highest one produced by the 0.125 VS rasio mix. While the ratio of FOG waste with FW at 0.3 and 0.5 only produce 128 ± 195 and 4 ± 1.45 mL CH4/gr VS, respectively. The ratio of 0.125 also demonstrates the highest COD reduction of 56% compared to the other ratio which indicates the 0.125 FOG and FW ratio can be implemented to utilize FOG waste and increase methane yield during anaerobic digestion process.


Energies ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 2101 ◽  
Author(s):  
David Valero ◽  
Carlos Rico ◽  
Blondy Canto-Canché ◽  
Jorge Domínguez-Maldonado ◽  
Raul Tapia-Tussell ◽  
...  

Nejayote (corn step liquor) production in Mexico is approximately 1.4 × 1010 m3 per year and anaerobic digestion is an effective process to transform this waste into green energy. The biochemical methane potential (BMP) test is one of the most important tests for evaluating the biodegradability and methane production capacity of any organic waste. Previous research confirms that the addition of conductive materials significantly enhances the methane production yield. This study concludes that the addition of granular activated carbon (GAC) increases methane yield by 34% in the first instance. Furthermore, results show that methane production is increased by 54% when a GAC biofilm is developed 10 days before undertaking the BMP test. In addition, the electroactive population was 30% higher when attached to the GAC than in control reactors. Moreover, results show that electroactive communities attached to the GAC increased by 38% when a GAC biofilm is developed 10 days before undertaking the BMP test, additionally only in these reactors Geobacter was identified. GAC has two main effects in anaerobic digestion; it promotes direct interspecies electron transfer (DIET) by developing an electro-active biofilm and simultaneously it reduces redox potential from −223 mV to −470 mV. These results suggest that the addition of GAC to biodigesters, improves the anaerobic digestion performance in industrial processed food waste.


1986 ◽  
Vol 18 (7-8) ◽  
pp. 239-248 ◽  
Author(s):  
Sung Ryong Ha ◽  
Dwang Ho Lee ◽  
Sang Eun Lee

Laboratory scale experiments were conducted to develop a mathematical model for the anaerobic digestion of a mixture of night soil and septic tank sludge. The optimum mixing ratio by volume between night soil and septic tank sludge was found to be 7:3. Due to the high solids content in the influent waste, mixed-liquor volatile suspended solids (MLVSS) was not considered to be a proper parameter for biomass concentration, therefore, the active biomass concentration was estimated based on deoxyribonucleic acid (DNA) concentration in the reactor. The weight ratio between acidogenic bacteria and methanogenic bacteria in the mixed culture of a well-operated anaerobic digester was approximately 3:2. The proposed model indicates that the amount of volatile acid produced and the gas production rate can be expressed as a function of hydraulic residence time (HRT). The kinetic constants of the two phases of the anaerobic digestion process were determined, and a computer was used to simulate results using the proposed model for the various operating parameters, such as BOD5 and volatile acid concentrations in effluent, biomass concentrations and gas production rates. These were consistent with the experimental data.


Sign in / Sign up

Export Citation Format

Share Document