An OxiTop® protocol for screening plant material for its biochemical methane potential (BMP)

A protocol was developed for determining the biochemical methane potential (BMP) of plant material using the OxiTop® system. NaOH pellets for CO2 absorption and different pretreatment methods were tested for their influence in the BMP test. The use of NaOH pellets in the headspace of the bottle negatively affected the stability of the test increasing the pH and inhibiting methanization. Sample comminution increased the biodegradability of plant samples. Our results clearly indicate the importance of test conditions during the assessment of anaerobic biodegradability of plant material, considering BMP differences as high as 44% were found. Guidelines and recommendations are given for screening plant material suitable for anaerobic digestion using the OxiTop® system.

Download Full-text

Biochemical Methane Potential of Cork Boiling Wastewater at Different Inoculum to Substrate Ratios

Applied Sciences ◽

10.3390/app11073064 ◽

2021 ◽

Vol 11 (7) ◽

pp. 3064

Author(s):

Roberta Mota-Panizio ◽

Manuel Jesús Hermoso-Orzáez ◽

Luis Carmo-Calado ◽

Gonçalo Lourinho ◽

Paulo Sérgio Duque de Brito

Keyword(s):

Anaerobic Digestion ◽

Biogas Production ◽

Treatment Plant ◽

Organic Substrates ◽

Biochemical Methane Potential ◽

Volatile Solids ◽

Working Volume ◽

Methane Potential ◽

Bmp Test ◽

Cork Industry

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.

Download Full-text

Anaerobic digestion of sunflower oil cake: a current overview

Water Science & Technology ◽

10.2166/wst.2012.586 ◽

2013 ◽

Vol 67 (2) ◽

pp. 410-417 ◽

Cited By ~ 4

Author(s):

M. A. De la Rubia ◽

V. Fernández-Cegrí ◽

F. Raposo ◽

R. Borja

Keyword(s):

Anaerobic Digestion ◽

Sunflower Oil ◽

Physical Structure ◽

Methane Yield ◽

Organic Loading Rate ◽

Biochemical Methane Potential ◽

In Series ◽

Methane Potential ◽

Bmp Test ◽

Sunflower Oil Cake

Due to the chemical and physical structure of a lignocellulosic biomass, its anaerobic digestion (AD) is a slow and difficult process. In this paper, the results obtained from a batch biochemical methane potential (BMP) test and fed-batch mesophilic AD assays of sunflower oil cake (SuOC) are presented. Taking into account the low digestibility shown during one-stage experiments the methane yield decreased considerably after increasing the organic loading rate (OLR) from 2 to 3 g VS L−1 d−1, SuOC was subjected to a two-stage AD process (hydrolytic-acidogenic and methanogenic stages), in two separate reactors operating in series where the methanogenic stage became acidified (with >1,600 mg acetic acid L−1) at an OLR as low as 2 g VS L−1 d−1. More recently, BMP assays were carried out after mechanical, thermal, and ultrasonic pre-treatments to determine the best option on the basis of the methane yield obtained.

Download Full-text

Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Water ◽

10.3390/w11050921 ◽

2019 ◽

Vol 11 (5) ◽

pp. 921 ◽

Cited By ~ 38

Author(s):

Jameson Filer ◽

Huihuang H. Ding ◽

Sheng Chang

Keyword(s):

Anaerobic Digestion ◽

Systematic Errors ◽

Research Progress ◽

Assay Method ◽

Biochemical Methane Potential ◽

Anaerobic Digestion Process ◽

Digestion Process ◽

Methane Potential ◽

Bmp Test ◽

Key Aspects

Biochemical methane potential (BMP) tests are widely used for characterizing a substrate’s influence on the anaerobic digestion process. As of 2018, there continues to be a lack of standardization of units and techniques, which impacts the comparability and validity of BMP results. However, BMP methods continue to evolve, and key aspects are studied to further eliminate systematic errors. This paper aims to update these key aspects with the latest research progress both to introduce the importance of each variable to those new to BMP measurements and to show the complexity required to design an accurate BMP test.

Download Full-text

Mesophilic Fermentation of SOMW in a Micro Pilot-Scale Anaerobic Digester

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.827.84 ◽

2013 ◽

Vol 827 ◽

pp. 84-90 ◽

Cited By ~ 3

Author(s):

Maurizio Carlini ◽

Sonia Castellucci ◽

Silvia Cocchi

Keyword(s):

Anaerobic Digestion ◽

Energy Production ◽

Biogas Production ◽

Ph Control ◽

Pilot Scale ◽

Biochemical Methane Potential ◽

Mediterranean Countries ◽

Methane Potential ◽

Environmentally Sound ◽

Bmp Test

One of the most promising processes to exploit Solid Olive-Mill Waste (SOMW) for energy production is anaerobic digestion. An experimental study has been carried out on SOMW and inoculum, consisting of Cattle Slurry Digested (CSD) and coming from an anaerobic digestion plant. A substrate with an optimal supply ratio equal to 2:1 has been investigated in a reactor at 37°C by analysing the biogas production. The Biochemical Methane Potential (BMP) test has been carried out, monitoring pH, biogas production (amount and composition). According to the tests results, SOMWs needed to be diluted and inoculated, moreover the pH control is foundamental in order to obtain a significant biogas production. Anaerobic digestion plant of SOMW should be promoted in Mediterranean countries as an environmentally sound option for waste management and energy production, since olive mills are very widespread agro-industries in this area.

Download Full-text

Enhancing Biochemical Methane Potential and Enrichment of Specific Electroactive Communities from Nixtamalization Wastewater using Granular Activated Carbon as a Conductive Material

Energies ◽

10.3390/en11082101 ◽

2018 ◽

Vol 11 (8) ◽

pp. 2101 ◽

Cited By ~ 11

Author(s):

David Valero ◽

Carlos Rico ◽

Blondy Canto-Canché ◽

Jorge Domínguez-Maldonado ◽

Raul Tapia-Tussell ◽

...

Keyword(s):

Activated Carbon ◽

Anaerobic Digestion ◽

Methane Production ◽

Production Capacity ◽

Granular Activated Carbon ◽

Green Energy ◽

Biochemical Methane Potential ◽

Methane Potential ◽

Bmp Test ◽

Main Effects

Nejayote (corn step liquor) production in Mexico is approximately 1.4 × 1010 m3 per year and anaerobic digestion is an effective process to transform this waste into green energy. The biochemical methane potential (BMP) test is one of the most important tests for evaluating the biodegradability and methane production capacity of any organic waste. Previous research confirms that the addition of conductive materials significantly enhances the methane production yield. This study concludes that the addition of granular activated carbon (GAC) increases methane yield by 34% in the first instance. Furthermore, results show that methane production is increased by 54% when a GAC biofilm is developed 10 days before undertaking the BMP test. In addition, the electroactive population was 30% higher when attached to the GAC than in control reactors. Moreover, results show that electroactive communities attached to the GAC increased by 38% when a GAC biofilm is developed 10 days before undertaking the BMP test, additionally only in these reactors Geobacter was identified. GAC has two main effects in anaerobic digestion; it promotes direct interspecies electron transfer (DIET) by developing an electro-active biofilm and simultaneously it reduces redox potential from −223 mV to −470 mV. These results suggest that the addition of GAC to biodigesters, improves the anaerobic digestion performance in industrial processed food waste.

Download Full-text

Optimizing anaerobic co-digestion of goat manure and cotton gin trash using biochemical methane potential (BMP) test and mathematical modeling

SN Applied Sciences ◽

10.1007/s42452-021-04706-1 ◽

2021 ◽

Vol 3 (8) ◽

Author(s):

Harjinder Kaur ◽

Raghava R. Kommalapati

Keyword(s):

Liquid Fraction ◽

Gompertz Model ◽

Biochemical Methane Potential ◽

Methane Potential ◽

Mathematical Equations ◽

Goat Manure ◽

Bmp Test ◽

Batch Bioreactors ◽

Modified Gompertz Model ◽

Cotton Gin

AbstractAnaerobic co-digestion is widely adopted to enhance process efficacy by balancing the C/N ratio of the feedstock while converting organic wastes to biomethane. Goat manure (GM) and cotton gin trash (CGT) were anaerobically co-digested in triplicate batch bioreactors. The process was optimized and evaluated utilizing mathematical equations. The liquid fraction of the digestate was analyzed for nitrate and phosphate. The co-digestions with 10 and 20% CGT having the C/N ratios of 17.7 and 19.8 yielded the highest and statistically similar 261.4 ± 4.8 and 262.6 ± 4.2 mL/gvs biomethane, respectively. The biodegradability (BD) of GM and CGT was 94.5 ± 2.7 and 37.6 ± 0.8%, respectively. The BD decreased proportionally with an increase in CGT percentage. The co-digestion having 10% CGT yielded 80–90% of biomethane in 26–39 d. The modified Gompertz model-predicted and experimental biomethane values were similar. The highest synergistic effect index of 15.6 ± 4.7% was observed in GM/CGT; 30:70 co-digestion. The concentration of nitrate and phosphate was lower in the liquid fraction of digestate than the feedstocks, indicating that these nutrients stay in the solid fraction. The results provide important insights in agro-waste management, further studies determining the effects of effluent application on plants need to be conducted.

Download Full-text

Determination of methane yield of cellulose using different experimental setups

Water Science & Technology ◽

10.2166/wst.2014.275 ◽

2014 ◽

Vol 70 (4) ◽

pp. 599-604 ◽

Cited By ~ 24

Author(s):

Bing Wang ◽

Ivo Achu Nges ◽

Mihaela Nistor ◽

Jing Liu

Keyword(s):

Water Column ◽

Test System ◽

Methane Yield ◽

Biochemical Methane Potential ◽

Methane Potential ◽

Bmp Test ◽

Potential Test

In this work, biochemical methane potential (BMP) tests with cellulose as a model substrate were performed with the aid of three manually operated or conventional experimental setups (based on manometer, water column and gas bag) and one automated apparatus specially designed for analysis of BMP. The methane yields were 340 ± 18, 354 ± 13, 345 ± 15 and 366 ± 5 ml CH4/g VS obtained from experimental setups with manometer, water column, gas bag, and automatic methane potential test system, which corresponded to a biodegradability of 82, 85, 83 and 88% respectively. The results demonstrated that the methane yields of cellulose obtained from conventional and automatic experimental setups were comparable; however, the methane yield obtained from the automated apparatus showed greater precision. Moreover, conventional setups for the BMP test were more time- and labour-intensive compared with the automated apparatus.

Download Full-text

Enhancing Methane Production from Spring-Harvested Sargassum muticum

Springer Proceedings in Energy - Energy and Sustainable Futures ◽

10.1007/978-3-030-63916-7_15 ◽

2021 ◽

pp. 117-123

Author(s):

Supattra Maneein ◽

John J. Milledge ◽

Birthe V. Nielsen

Keyword(s):

Anaerobic Digestion ◽

Pharmaceutical Industry ◽

Brown Seaweed ◽

Sargassum Muticum ◽

Biofuel Production ◽

Biochemical Methane Potential ◽

Seaweed Species ◽

Invasive Seaweed ◽

Methane Potential ◽

Aqueous Meoh

AbstractSargassum muticum is a brown seaweed which is invasive to Europe and currently treated as waste. The use of S. muticum for biofuel production by anaerobic digestion (AD) is limited by low methane (CH4) yields. This study compares the biochemical methane potential (BMP) of S. muticum treated in three different approaches: aqueous methanol (70% MeOH) treated, washed, and untreated. Aqueous MeOH treatment of spring-harvested S. muticum was found to increase CH4 production potential by almost 50% relative to the untreated biomass. The MeOH treatment possibly extracts AD inhibitors which could be high-value compounds for use in the pharmaceutical industry, showing potential for the development of a biorefinery approach; ultimately exploiting this invasive seaweed species.

Download Full-text