Biotechnological methods of selection in vitro forms of sage, resistant to abiotic stresses

2019 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
I.V. Stavtseva ◽  
◽  
Keyword(s):  
Abiotic Stresses

scholarly journals Evaluation of Indigenous Olive Biocontrol Rhizobacteria as Protectants against Drought and Salt Stress

2021 ◽  
Vol 9 (6) ◽  
pp. 1209
Author(s):  
Nuria Montes-Osuna ◽  
Carmen Gómez-Lama Cabanás ◽  
Antonio Valverde-Corredor ◽  
Garikoitz Legarda ◽  
Pilar Prieto ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Biochemical Parameters ◽  
Biocontrol Agent ◽  
Negative Effects ◽  
Experimental Conditions ◽  
Acds Gene ◽  
Drought And Salt Stress ◽  
Physiological And Biochemical

Stress caused by drought and salinity may compromise growth and productivity of olive (Olea europaea L.) tree crops. Several studies have reported the use of beneficial rhizobacteria to alleviate symptoms produced by these stresses, which is attributed in some cases to the activity of 1-aminocyclopropane-1-carboxylic acid deaminase (ACD). A collection of beneficial olive rhizobacteria was in vitro screened for ACD activity. Pseudomonas sp. PICF6 displayed this phenotype and sequencing of its genome confirmed the presence of an acdS gene. In contrast, the well-known root endophyte and biocontrol agent Pseudomonas simiae PICF7 was defective in ACD activity, even though the presence of an ACD-coding gene was earlier predicted in its genome. In this study, an unidentified deaminase was confirmed instead. Greenhouse experiments with olive ‘Picual’ plants inoculated either with PICF6 or PICF7, or co-inoculated with both strains, and subjected to drought or salt stress were carried out. Several physiological and biochemical parameters increased in stressed plants (i.e., stomatal conductance and flavonoids content), regardless of whether or not they were previously bacterized. Results showed that neither PICF6 (ACD positive) nor PICF7 (ACD negative) lessened the negative effects caused by the abiotic stresses tested, at least under our experimental conditions.

scholarly journals Seed-Biopriming of Durum Wheat with Diazotrophic Plant Browth Promoting Bacteria (PGPB) Enhanced Tolerance to Fusarium Head Blight (FHB) and Salinity Stress

2019 ◽  
Author(s):  
Adel Hadj Brahim ◽  
Mouna Jlidi ◽  
Lobna Daoud ◽  
Manel Ben-Ali ◽  
Asmahen Akremi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Durum Wheat ◽  
Abiotic Stresses ◽  
Growth Promotion ◽  
Head Blight ◽  
Biotic And Abiotic Stresses ◽  
Plant Growth Promoting ◽  
Seed Biopriming

Abstract Background The use of bioinoculants based on plant growth-promoting bacteria (PGPB) to promote plant growth under biotic and abiotic stresses is in full expansion. To our knowledge much work has not been, thus far, done on seed-biopriming of durum wheat for tolerance to biotic and abiotic stresses. In the present work, we report detailed account of the effectiveness a potent bacterial strain with proven plant growth-promoting ability and antimicrobial activity. The isolate was selected following screening of several bacterial strains isolated from halophytes that grow in a coastal saline soil in Tunisia for their role in enhancing durum wheat tolerance to both salinity stress and head blight disease.Results Accordingly, Bacillus strains MA9, MA14, MA17 and MA19 were found to have PGPB characteristics as they produced indole-3-acetic acid, siderophores and lytic enzymes, fixed free atmospheric nitrogen, and solubilized inorganic phosphate, in vitro . The in vivo study that involved in planta inoculation assays under control (25 mM NaCl) and stress (125 mM NaCl) conditions indicated that all PGPB strains significantly ( P < 0.05) increased the total plant length, dry weight, root area, seed weight, nitrogen, protein and total mineral content. On the other hand, strain MA17 reduced Fusarium Head Blight (FHB) disease incidence in wheat explants by 64.5%, showing that the strain has antifungal activity as was also displayed by in vitro inhibition study.Conclusions Both in vitro and in vivo studies showed that MA9, MA14 MA9, MA14, MA17 and MA19 strains were able to play the PGPB role. Yet, biopriming with Bacillus strain MA17 offered the highest bioprotection against FHB, plant growth promotion, and salinity tolerance. Hence, the MA17 strain should further be evaluated under field condition and formulated for commercial production. Besides, the strain could further be evaluated for its potential role in bioprotection and growth promotion of other crop plants. We believe, the strain has potential to significantly contribute to wheat production in the arid and semi-arid region, especially the salt affected Middle Eastern Region, besides its potential role in improving wheat production under biotic and abiotic stresses in other parts of the world.

Molecular characterization, heterologous expression and resistance analysis of OsLEA3-1 from Oryza sativa

Biologia ◽  
2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Tingzhang Hu ◽  
Junnian Yang ◽  
Yongwei Yang ◽  
Yingmei Wu
Keyword(s):  
Fusion Protein ◽  
Heterologous Expression ◽  
Abiotic Stresses ◽  
Structure Prediction ◽  
Bioinformatics Analysis ◽  
Secondary Structure Prediction ◽  
Mature Embryo ◽  
Lactate Dehydrogenase Activity ◽  
E Coli

AbstractLate embryogenesis abundant (LEA) proteins in organisms are closely associated with resistance to abiotic stresses. Here we characterized a rice LEA protein, OsLEA3-1, by bioinformatics analysis and heterologous expression in Escherichia coli. Bioinformatics analysis showed that OsLEA3-1 contains a 603-bp open reading frame encoding a putative polypeptide of 200 amino acids, which contains a “LEA_4” motif at positions 5–48 and belongs to a typical group 3 LEA. OsLEA3-1 polypeptide is rich in Ala, Lys, and Thr, but depleted in Cys, Pro, and Trp residues; and is strongly hydrophilic. Secondary structure prediction showed that OsLEA3-1 polypeptide contained an α-helical domain in positions 4-195 but not any β-sheet domain. OsLEA3-1 gene can express in shoot and root of germinating seeds, seedling, panicles, mature embryo, seed, and callus; and was also up-regulated by ultraviolet (UV), heat, cold, salt, and emergency drought. OsLEA3-1 gene was introduced into E. coli. A fusion protein of about 28.03 kDa was expressed in recombinant E. coli cells after the induction by isopropylthio-β-D-galactoside. Compared with control E. coli cells harbouring pET30a, the accumulation of the OsLEA3-1 fusion protein increased the tolerance of the E. coli recombinants under diverse abiotic stresses: high salinity, metal ions, hyperosmotic, heat, and UV radiation. The OsLEA3-1 has the ability to protect the lactate dehydrogenase activity under heating, drying, and MnCl2 treatment in vitro. The findings suggested that the OsLEA3-1 gene may contribute to the ability of adapting to stressful environments of plants.

scholarly journals 264. Biofilm cells of Trichosporon asahii Show Higher Resistance Than Planktonic Cells to Various Abiotic Stresses

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S146-S147
Author(s):  
Yang Metok ◽  
Supram Hosuru Subramanya ◽  
Niranjan Nayak
Keyword(s):  
Congo Red ◽  
Abiotic Stresses ◽  
Wall Stress ◽  
Antifungal Drugs ◽  
Stress Factors ◽  
Planktonic Cells ◽  
Biofilm Inhibition ◽  
Trichosporon Asahii ◽  
Sodium Bisulfate

Abstract Background Biofilms of Trichosporon asahii are known to resist the effects of antifungal drugs, but the study of their susceptibility to various abiotic stresses remains sparse. This study was thus undertaken to compare the level of in vitro resistance of T. asahii biofilm and planktonic cells to various stress factors. Methods In this study, one T. asahii clinical isolate identified by amplifying IGS1 sequencing and one reference strain (NCCPF940033) were used. Biofilm and planktonic cells of T. asahii were exposed to increasing concentrations of NaCl (0.5–6 M) and d-sorbitol (3–13 M) for inducing osmotic stress; H2O2 (5–50 mM), menadione sodium bisulfate (0.048–100 mM) and ox bile (1–12%) for oxidative stress; PH 1 to 13 for PH stress; congo red (600–10,000 µg/mL) for cell wall stress; CuSO4·5H2O/ZnSO4/FeSO4 (1.25–2,560 mM) and MgSO4 (250–3,000 mM) for metal stress. The biomass and metabolic activity of biofilms were quantitatively determined by crystal violet method and XTT reduction assay, respectively. Further, spot assay of serially diluted (10–1 to 10–6) planktonic cells was performed on agar plates containing stress and non-stress control to determine relative percentage growth of strains. Results Biofilm cells of both the strains exhibited significantly higher (ANOVA) stress resistance than planktonic cells and on an average showed at least 100 times more resistant to stresses than planktonic cells [Minimum Biofilm Eradication Concentration (MBEC) vs. Minimum Inhibitory Concentration (MIC)]; H202 >50 mM vs. 10 mM, Ox bile >12% vs. 2%, Menadione >100 mM vs. 0.39 mM, Zn/Fe/Cu >2,560 mM vs. 10 mM, Mg >3,000 mM vs. 1,000 mM, NaCl >6 M vs. 1.5 M, d-sorbitol >13 M vs. 5 M and Congo red >10,000 µg/mL vs. 800 µg/mL. Besides optimal PH 5–10, extreme acidic and alkaline PH led to complete inhibition of viable planktonic cells. Highest biomass reduction (77.2%) and highest viability inhibition (69%) of biofilm were observed at PH 3 and 13, respectively. Menadione reduced 86.9% biomass and 89.3% viability which accounted the highest biofilm inhibition. Conclusion This is the first report on comparing the susceptibility of planktonic and biofilm T. asahii cells to various stress factors. The increased resistance of T. asahii biofilm may serve as a survival advantage against the host adversity. Disclosures All authors: No reported disclosures.

scholarly journals Protection of in-vitro grown Arabidopsis seedlings against abiotic stresses

2008 ◽  
Vol 95 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Geert-Jan de Klerk ◽  
Paweena Pumisutapon
Keyword(s):  
Abiotic Stresses

scholarly journals The ploidy level of triticale plant regenerants, obtained by in vitro selection for resistance to abiotic stresses

2018 ◽  
Vol 22 ◽  
pp. 305-310
Author(s):  
S. V. Pykalo ◽  
O. V. Dubrovna
Keyword(s):  
Ploidy Level ◽  
Abiotic Stresses ◽  
In Vitro Selection ◽  
Selective Advantage ◽  
Cytological Analysis ◽  
Winter Triticale ◽  
Somaclonal Variability ◽  
Aneuploid Chromosome ◽  
Selection For

Aim. To analyze the ploidy level of plant regenerants of winter triticale, obtained by in vitro selection for resistance to osmotic and salt stresses. Methods. By cytological analysis and flow cytometry methods there was determined the ploidy level in the plant regenerants of winter triticale obtained by in vitro selection for resistance to abiotic stresses. Results. The somaclonal variability of plant regenerants of winter triticale resistant to osmotic and salt stresses by ploidy level was observed. The cytological instability of resistant’s regenerants was revealed that was due in appearance of aneuploidy plants. Plants with aneuploid chromosome set (38–41) were characterized by reduced viability and abnormal generative organs resulting they are not formed normal ears and not received seeds. Conclusions. Among the obtained regenerants euploids were in most cases indicating a selective advantage of hexaploid cells in ability to morphogenesis. Keywords: Triticale, plant regenerants, cytological analysis, aneuploids, abiotic stresses.

scholarly journals Improving the Health-Benefits of Kales (Brassica oleracea L. var. acephala DC) through the Application of Controlled Abiotic Stresses: A Review

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2629
Author(s):  
Erika Ortega-Hernández ◽  
Marilena Antunes-Ricardo ◽  
Daniel A. Jacobo-Velázquez
Keyword(s):  
Brassica Oleracea ◽  
Abiotic Stresses ◽  
Degenerative Diseases ◽  
Cruciferous Vegetable ◽  
Starting Point ◽  
Health Promoting ◽  
Therapeutic Properties ◽  
Brassica Oleracea L

Kale (Brassica oleracea L. var. acephala DC) is a popular cruciferous vegetable originating from Central Asia, and is well known for its abundant bioactive compounds. This review discusses the main kale phytochemicals and emphasizes molecules of nutraceutical interest, including phenolics, carotenoids, and glucosinolates. The preventive and therapeutic properties of kale against chronic and degenerative diseases are highlighted according to the most recent in vitro, in vivo, and clinical studies reported. Likewise, it is well known that the application of controlled abiotic stresses can be used as an effective tool to increase the content of phytochemicals with health-promoting properties. In this context, the effect of different abiotic stresses (saline, exogenous phytohormones, drought, temperature, and radiation) on the accumulation of secondary metabolites in kale is also presented. The information reviewed in this article can be used as a starting point to further validate through bioassays the effects of abiotically stressed kale on the prevention and treatment of chronic and degenerative diseases.

The effect of abiotic stresses on carbohydrate status of olive shoots (Olea europaea L.) under in vitro conditions

2007 ◽  
Vol 164 (2) ◽  
pp. 174-184 ◽  
Author(s):  
Alžběta Rejšková ◽  
Lenka Patková ◽  
Eva Stodůlková ◽  
Helena Lipavská
Keyword(s):  
Abiotic Stresses ◽  
Olea Europaea ◽  
Olea Europaea L ◽  
Carbohydrate Status ◽  
Olea Europaéa

scholarly journals Stress causing dynamic changes of four phytohormones in tobacco and tomato: A GC-MS analysis

1970 ◽  
pp. 16-22
Author(s):  
Chaitali Roy ◽  
Smriti Ranjan Maji ◽  
Tapas Kumar Ghose, Swati Gupta Bhattacharya
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Abiotic Stresses ◽  
Plant Responses ◽  
Abiotic Stress Response ◽  
Dynamic Changes ◽  
Signalling Molecules ◽  
Hormone Profiling ◽  
Leaf Tissues

Many analytical procedures have been developed to determine the importance of phytohormones in different plants. The work reported here provides a sensitive, accurate and readily accessible gas chromatography coupled to mass spectrometry (GC-MS) technique designed for the simultaneous quantitation of phytohormones indole-3-aceticacid (IAA), abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). These signalling molecules were analysed in two different plants, Tomato and Tobacco grown in vitro. The protocol designed to assess the dynamic changes in endogenous concentrations of hormones to study plant responses to abiotic stresses in leaf tissues. A hormone profiling is obtained from leaves of plants exposed to salt stress show that different plant hormones are involved in diverse physiologicsl processes. Crosstalk between these hormones result in synergetic or antagonic interactions which have important roles to play in abiotic stress response.

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