scholarly journals Antinociceptive and Anti-inflammatory Effects of Methanolic Extract of Laurencia caspica

2020 ◽  
Vol 5 (2) ◽  
pp. 54-60
Author(s):  
Katayoon Karimzadeh ◽  
Mahdiyeh Ramzanpoor ◽  
Shadi Keihankhadiv
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Pain Treatment ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Second Phases ◽  
Anti Inflammatory

Introduction: Seaweeds are valuable resources for the discovery of efficient and safe drugs for pain treatment. In the present investigation, we evaluated the antinociceptive and anti-inflammatory properties of, methanolic extract of Laurencia caspica, a red algae, in mice models. Methods: The analgesic effect of methanolic extract of L. caspica was assessed by hot-plate and acetic acid-induced writhing tests in male Swiss albino mice (weight = 20-25 g). The anti-inflammatory activity of methanolic extract of L. caspica was also evaluated by formalin-induced ear edema and xylene-induced paw edema tests. Results: The total flavonoid content of the extract was estimated as 0.0537 mg quercetin/g extract. Both first and second phases of the nociception were significantly inhibited at a dose of 120 mg/kg of methanolic extract of L. caspica. The observed anti-inflammatory effect was dose-dependent. Acetic acid-induced writhing test and hot plate test showed that the extract significantly reduced pain in all evaluated doses (15, 30, 60, and 120 mg/kg). The antinociceptive activity of the methanolic extract was significantly reduced by naloxone (4 mg/kg). Moreover, the extract significantly reduced paw edema at the dose of 120 mg/kg in all the animals. Conclusion: Methanolic extract of L. caspica exhibited central analgesic effect, as well as anti-inflammatory activity probably due to the presence of constituents like flavonoids and triterpenoids.

scholarly journals INVESTIGATION OF ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF AQUEOUS EXTRACTS OF ARTEMISIA AFRA IN WISTAR RATS

2018 ◽  
Vol 11 (12) ◽  
pp. 190 ◽  
Author(s):  
Mavuto Gondwe ◽  
Anda Mpalala ◽  
Lusanda Zongo ◽  
David Kamadyaapa ◽  
Eugene Ndebia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Effects ◽  
Control Group ◽  
Paw Edema ◽  
Hot Plate ◽  
Traditional Practice ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Antinociceptive Effects ◽  
Anti Inflammatory

Objective: The objective of the study was to evaluate the anti-inflammatory and antinociceptive effects of Artemisia afra.Methods: Animals were randomly divided into five groups of six animals each and administered with normal saline (2 ml/kg), indomethacin (10 mg/ kg), and A. afra at doses of 100, 200, and 400 mg/kg, respectively. For the anti-inflammatory activity, carrageenan-induced paw edema was used while the hot plate and acetic acid induced-writhing tests were used to assess the antinociceptive activity.Results: Pretreatment with A. afra at a dose of 100 mg/kg did not show any significant biological effects (p>0.05) for any of the three tests, when compared against saline-treated control group. At a dose of 200 mg/kg, A. afra demonstrated significant effects (p<0.01), during the 5th h reducing carrageenan-induced paw edema by 12%. The highest dose (400 mg/kg) of A. afra demonstrated more potent effects by decreasing the carrageenan-induced paw swelling (p<0.001–0.05) during the 3rd, 4th, and 5th h, by up to 38% when compared against saline-treated control group. Both the 200 and 400 mg/kg, A. afra doses achieved a significant increase (p<0.05) in reaction time in the hot plate test. In the acetic acid-induced writhing test, pretreatment with A. afra (400 mg/kg) significantly reduced pain by 39% (p<0.01) by comparison with the saline control.Conclusion: Experimental data demonstrated that aqueous extract of A. afra possesses anti-inflammatory and antinociceptive properties in experimental acute inflammation and pain. These findings support the usage of A. afra in managing inflammation and pain in traditional practice.

Anti-inflammatory Activity of CML-1: An Herbal Formulation

2005 ◽  
Vol 33 (01) ◽  
pp. 29-40 ◽  
Author(s):  
Hyun-Ae Eum ◽  
Woo-Yong Lee ◽  
Sung-Ho Kim ◽  
Joo-Young Kim ◽  
Sang-Won Park ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dose Level ◽  
Inflammatory Diseases ◽  
Inflammatory Activity ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Herbal Formulation ◽  
Cinnamomi Cortex ◽  
Anti Inflammatory ◽  
Paeoniae Radix

CML-1 is a purified extract from a mixture of 13 Oriental herbs (Achyranthis Radix, Angelicae Gigantis Radix, Cinnamomi Cortex Spissus, Eucommiae Cortex, Glycyrrhizae Radix, Hoelen, Lycii Fructus, Paeoniae Radix, Rehmanniae Radix Preparata and Atractylodis Rhizoma, Zingiberis Rhizoma, Zizyphi Semen, Acori Graminei Rhizoma) that have been widely used for the treatment of inflammatory diseases in Asia. The aim of this study was to investigate the anti-inflammatory and analgesic potential of CML-1. The animals used in this study were administered either vehicle or CML-1 (30, 100, 300 and 600 mg/kg) orally. The vascular permeability induced by acetic acid was significantly reduced by CML-1 in all doses. The swelling of the rat's hind paw induced by carrageenan was significantly inhibited by CML-1 in doses of 100, 300 and 600 mg/kg. In the case of rheumatoid arthritis induced by complete Freund's adjuvant in rats, the treatment with CML-1 at a dose level of 300 mg/kg inhibited edema. CML-1 at a dose level of 600 mg/kg inhibited acetic acid-induced writhing syndrome, however it did not have any anti-nociceptive action in the Randall-Selitto assay or the hot plate test. Our findings suggest that CML-1 has a potent anti-inflammatory activity.

scholarly journals Evaluation of Analgesic and Anti-Inflamatory Activity of Ventilago calyculata

2015 ◽  
Vol 9 (1) ◽  
pp. 43-47
Author(s):  
G. Pranaya ◽  
P. Venkata Smitha ◽  
N. Srinivasa Reddy ◽  
R. Vinay ◽  
Ch. Murali Mohan ◽  
...  
Keyword(s):  
Formalin Test ◽  
Methanolic Extract ◽  
Life Sciences ◽  
Inflammatory Activity ◽  
Tail Flick ◽  
Paw Edema ◽  
Hot Plate ◽  
Plate Method ◽  
Ear Edema ◽  
Anti Inflammatory

Ventilago calyculata was traditionally used to treat sprains and pain. The purpose of this study was to evaluate the analgesic and anti-inflammatory activity of methanolic extract of bark of Ventilago calyculata (MVC). For evaluating analgesic activity, Hot plate method, Tail-flick method, Formalin test and Writhing methods were used. For evaluating anti-inflammatory activity Carrageenan induced paw edema and Xylene induced ear edema methods were used. Administration of MVC (100 and 200mg/kg) significantly reduced the total number of writhings in Acetic acid induced writhing method. In Hot plate and Tail-Flick methods there was a significant increase in baseline. There was a significant reduction in ear edema in Xylene induced ear edema method. The results suggest that the methanolic extract of bark of Ventilago calyculata might have analgesic and anti-inflammatory activity.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11925 International Journal of Life Sciences Vol.9(1) 2015 43-47

scholarly journals STUDY OF THE ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF A NEW NON-OPIOID ANALGESIC BASED ON A SELECTIVE INHIBITOR OF TRPA1 ION CHANNELS

2019 ◽  
Vol 26 (1) ◽  
pp. 77-87
Author(s):  
Evgeniya A. Beskhmelnitsyna ◽  
Mikhail V. Pokrovskii ◽  
Aleksandr A. Dolzhikov ◽  
Tat’yana V. Avtina ◽  
Nina I. Zhernakova ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Conflict Of Interest ◽  
Inflammatory Activity ◽  
Laboratory Mice ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Reference Drug ◽  
Plate Test ◽  
Analgesic Effects ◽  
Anti Inflammatory

The aim is to study the analgesic and anti-inflammatory activity of an innovative molecule under the ZC02-0012 laboratory code belonging to the group of selective blockers of the TRPA1 ion channel.Materials and methods. The study of the ZC02-0012 analgesic activity was carried out by the hot plate and acetic acid writhing tests. The hot plate test was performed on white laboratory mice. ZC02-0012 substance was administered in doses of 1, 3 and 9 mg/kg. Ketorol at a dose of 3.48 mg/kg was chosen as the reference drug. To study the analgesic effects in the hot plate test, intact white laboratory mice were placed on a preheated at 55°C plate to determine the base level of pain sensitivity. Then the test substances were administered to the animals divided into groups, and the hot plate test was repeated after 30, 60, 90 and 120 minutes. To study the analgesic effects in the test of acetic acid writhing, white laboratory rats were injected with the ZC02-0012 substance at doses of 0.46, 1.38, and 4.15 mg/kg. Ketorol at a dose of 1.6 mg/kg was chosen as the reference drug. 30 minutes after intramuscular injection and 60 minutes after intragastric administration, the intraperitoneal administration of a 0.75% solution of acetic acid was carried out at the rate of 1 ml per 100 g of animal weight. Counting the number of writhings started 15 minutes after the injection of acetic acid and continued for 30 minutes. The anti-inflammatory effects of the ZC02-0012 substance were studied using a model of acute exudative inflammation in response to the subplantar introduction of 0.02 ml of 2% formaldehyde aqueous solution into the right hind paw of the mouse. 4 hours after the phlogistic introduction, the mass of the edematous paw was evaluated. ZC02-0012 substance at doses of 1, 3 and 9 mg/kg and the Diclofenac reference drug at a dose of 13.91 mg/kg were administered intragastrically or intramuscularly 45 minutes before the introduction of phlogistic. The presence and severity of anti-inflammatory activity was judged by the inhibitory effect, expressed in percentage.Results. The analgesic activity of ZC02-0012 is found to exceed that for Ketorol in both the intramuscular and intragastric ways of administration according to the results of hot plate tests in doses of 3 and 9 mg/kg and acetic acid writhing in doses of 1.38 and 4.15 mg/kg, while the anti-inflammatory activity of ZC02-0012 at doses of 3 and 9 mg/kg is shown to be comparable to that of Diclofenac. Conclusion. The innovative molecule under the ZC02-0012 laboratory code is established to exhibit a pronounced analgesic and anti-inflammatory activity, thus being prospective for further research.Conflict of interest: the authors declare no conflict of interest.

Effects of aloperine on acute and inflammatory pain models in mice

2015 ◽  
Vol 8 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Yang Yang ◽  
Shao-Ju Jin ◽  
Hong-Ling Wang ◽  
Yu-Xiang Li ◽  
Juan Du ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Inflammatory Pain ◽  
Tail Flick ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Tail Flick Test ◽  
Plate Test ◽  
Tnf Α ◽  
Anti Inflammatory

AbstractBackground and objectivesAloperine (ALO) is an alkaloid compound and presents in several medicinal plants. This study was undertaken to investigate the anti-nociceptive and anti-inflammatory activities of ALO on various chemical- and thermal-induced hypersensitivity models in mice.MethodsThe anti-nociceptive effect of ALO was evaluated using acetic acid-induced writhing test, hot plate test, tail flick test, formalin test, ear swelling test, locomotor activity test, rota-rod test and carrageenan-induced paw edema test in mice. Inflammatory cytokines including interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β) and prostaglandin E2 (PGE2) expression were examined in ALO- and vehicle-treated mice.ResultsThe results showed that ALO significantly attenuated acetic acid-induced writhing numbers in mice in a dose-dependent manner. ALO showed no effect on prolonging latency in the hot plate test and the tail-flick test. ALO showed analgesic activity in the inflammatory phase of formalin-induced pain. Its anti-inflammatory effect was also confirmed in the ear-swelling test. In the carrageenan-induced paw edema model, ALO significantly and dose-dependently reduced the carrageenan-induced paw edema, decreased the contents of TNF-α IL-1β and PGE2, but increased the IL-10 production. On the other hand, ALO showed no influence on the rota-rod performance time or on spontaneous locomotor activity.ConclusionIt is concluded that ALO has both anti-inflammatory and analgesic effects, especially in the field of inflammation pain.ImplicationsOur findings support the hypothesis that ALO ameliorates inflammatory pain induced by chemical and thermal stimuli and provides a scientific basis for the resource development and clinical use of aloperine.

scholarly journals Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Akinyinka O. Alabi ◽  
Abayomi M. Ajayi ◽  
Osarume Omorogbe ◽  
Solomon Umukoro
Keyword(s):  
Acetic Acid ◽  
Aqueous Extract ◽  
Psidium Guajava ◽  
Antinociceptive Activity ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Promising Alternative ◽  
Plate Test ◽  
Ocimum Gratissimum ◽  
Anti Inflammatory

Abstract Background To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.

scholarly journals Evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Enhydra fluctuans on male wistar rats

2021 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Kishor Kumar Roy ◽  
Md. Kabirul Islam Mollah ◽  
Md. Masud Reja ◽  
Dibyendu Shil ◽  
Ranjan Kumar Maji
Keyword(s):  
Wistar Rats ◽  
Pain Threshold ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Hot Plate ◽  
Plate Method ◽  
Traditional System ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Enhydra fluctuations an edible semi-aquatic vegetable plant are widely used in the traditional system of medicine. Ethanolic extract of Enhydra fluctuans was screened for analgesic & anti-inflammatory activity. Analgesic activity was evaluated by the hot plate method and anti-inflammatory activity was evaluated by formalin induce paw edema in Wistar rats (male). Ethanolic extract dose of 300 mg/kg reduce formalin induce paw inflammation and also increased the pain threshold in rats evidenced by hot plate method. The experimental results concluded that Enhydra fluctuans have significant analgesic and anti-inflammatory activity may due to flavonoid and phenolic compound content.

scholarly journals Preparation and Analysis of New 1,3,5-Trisubstituted-2-Pyrazolines Derivative for Their Analgesic Potential

2021 ◽  
pp. 153-164
Author(s):  
Jitendra Kumar Chaudhary ◽  
Alok Pal Jain ◽  
O. P. Tiwari
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Latency Time ◽  
Hot Plate ◽  
Phenyl Hydrazine ◽  
Hot Plate Test ◽  
Test Technique ◽  
Plate Test ◽  
Withdrawal Latency ◽  
In Series

The goal of the study was to develop, synthesise, and characterise a novel 1,3,5-trisubstituted-2-pyrazolines derivative, as well as to assess its analgesic potential. The reaction of chalcone derivatives with 4-hydrazinylbenzene sulfonamide hydrochloride and phenyl hydrazine hydrochloride yielded 1,3,5-tri-substituted-2-pyrazolines derivatives. The IR, 1HNMR, and mass spectrum analyses were used to characterise a total of sixteen substances. Analgesic activity of the proposed substances has been tested. The analgesic effect of the produced compounds was tested using two methods: the hot plate test technique and acetic acid induced writhing in mice. To compare the effectiveness, pentazocine and acetyl acetic acid were utilised as reference drugs. The hot plate test technique and acetic acid induced writhing in mice were used to assess the analgesic effect of the 16 produced chemical series A1-A8, and B1-B8. The evaluation's outcomes were viewed using Pentazocine and acetyl acetic acid as the standard drugs. In a 90-minute hot plate test, compounds A2 (10.30 s), A4 (9.45 s), A7 (11.65 s), and A8 (11.26 s) showed a delay in paw withdrawal latency time. Compounds B2 (9.10 s) and B7 (10.42 s) prolong the paw withdrawal latency time after 90 minutes in series B1-B8, reduce the pain feeling, and inhibit pain induced by heat methods. Compounds A2, A5, A6, A7, and A8 from Series A1-A8 showed 83.00, 76.01, 80.34, 86.99, 88.15 percent inhibition, substantially (p0.05 and p0.001, respectively), and decreased the number of wriths caused by 0.6 percent acetic acid at a dosage of 10 mg/kg. Acetylsalicylic acid (10 mg/kg) appears to be more successful in lowering the number of wriths, with a 99.0% reduction in the number of wriths (p0.001). B1, B3, and B4 have the least amount of active activity. These all finding suggest that these synthesized compounds have the potential as analgesic agent.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

Antinociceptive Effect of Clomipramine Through Interaction with Serotonin 5-Нт2 And 5-Нт3 Receptor Subtypes

Folia Medica ◽  
2012 ◽  
Vol 54 (4) ◽  
pp. 69-77 ◽  
Author(s):  
Ilia D. Kostadinov ◽  
Delian P. Delev ◽  
Ivanka I. Kostadinova
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Antinociceptive Effect ◽  
Positive Control ◽  
Control Group ◽  
Receptor Subtypes ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Acid Test

Abstract INTRODUCTION: Tricyclic antidepressants are used in the treatment of various pain syndromes. The antidepressant clomipramine inhibits predominantly the reuptake of serotonin in the central nervous system. The mechanism of its analgesic effect is not fully understood. The AIM of the present study was to find experimentally any dose-effect dependence in the analgesic effect of clomipramine and the involvement of the 5-НТ2 and 5-НТ3 receptors in the mechanism of this effect. Material and methods: Fifty male Wistar rats were used in the study allocated to five groups (10 animals each): a saline treated control group, one positive control group treated with metamizole and three experimental groups treated with intraperitoneally administered clomipramine in doses of 5, 10 and 20 mg/kg bw, respectively. To study the role of 5-НТ2 and 5-НТ3 receptors in this effect we used another five groups (10 animals each): control, positive control and three experimental groups treated with clomipramine only, clomipramine and granisetrone and clomipramine and cyproheptadine, respectively. Three nociceptive tests were used: the hot plate test, analgesimeter and the acetic acid-induced writhing test. To gauge the antinociceptive action we used the increased latency in the hot plate test expressed as maximum possible effect % (%MPE), the increase in paw pressure to withdraw the hind paw in analgesimeter and decrease in the number of spinal cord writhes in the acetic acid test. RESULTS: Clomipramine in a dose of 20 mg/kg bw significantly increased the %MPE in hot plate test and the pressure to withdraw the hind paw in the analgesimeter when compared with the control. In the acetic acid test clomipramine decreased non-significantly the number of writhes compared with the controls. Granisetrone reduced non-significantly the antinociceptive effect of clomipramine in all tests. Cyproheptadine potentiated the analgesic effect of clomipramine in acetic acid test and decreased it significantly in the hot plate test. In analgesimeter cyproheptadine decreased significantly the paw pressure to withdraw the tested hind paw at 1 hour and non-significantly at 2 hours. CONCLUSION: Clomipramine in the dose of 20 mg/kg bw has a pronounced antinociceptive affect towards thermal and mechanical pain stimulation. The 5-HT2 and 5-HT3 receptor subtypes are very likely involved in the mechanism of this effect.

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