scholarly journals Interactive effect of 6 weeks of High-intensity interval training and quercetin supplementation on expression of some tumor angiogenesis markers in female mice with breast cancer

2021 ◽  
Vol 43 (1) ◽  
pp. 142-151
Author(s):  
Zohreh Jalali ◽  
Fereshteh Shahidi ◽  
abbas ali Gaeini ◽  
Shaban Alizadeh ◽  
Alireza Ramezani
Keyword(s):  
Breast Cancer ◽  
Tumor Angiogenesis ◽  
High Intensity ◽  
Breast Cancer Cells ◽  
Tumor Tissue ◽  
Statistical Tests ◽  
Interactive Effect ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
High Intensity Interval

Background: About angiogenesis, metastasis of breast cancer and exercise, Many studies have been done in recent decades to better understand of regulatory mechanisms. The purpose of this study was to investigate the Interactive effect of high-intensity interval training (HIIT) and quercetin supplementation (Q) on the expression of key angiogenic factors in tumor tissue of mouse with breast cancer. Methods: Twenty-four female Balb/C mouse were injected with estrogen receptor-dependent breast cancer cells MC4L2 and then divided into three groups of tumor (T), tumor + of high-intensity interval training (TH) and tumor + high-intensity interval training + quercetin (THQ). The TH and THQ groups ran the treadmill 3 days a week for 6 weeks and each session 1 hour. The THQ group received 110 mg / kg quercetin solution for 6 weeks, 3 days a week with training. After completion of the work, the mouse were sacrificed and their tumor tissue removed and frozen in liquid nitrogen And stored at -70 ° C. Expression of TIE-2 and VEGF-A genes was measured by REAL TIME-PCR. ΔCt, ΔΔCt, and Fold change were calculated with the relevant statistical tests at the significant level (P <0.05) by GENEX software. Results: The results showed that THQ interaction significantly decreased the expression of TIE-2 and VEGF-A genes in TH and THQ groups compared to T group. In addition, quercetin in THQ group significantly decreased TIE-2 and VEGF-A gene expression compared to TH group. Conclusion: interaction of HIIT and Q supplementation are probably effective in inhibiting tumor angiogenesis.

scholarly journals Treatment-induced tumor cell apoptosis following high-intensity interval training and saffron aqueous extract in mice with breast cancer

2021 ◽  
Author(s):  
F. Ahmadabadi ◽  
M. Saghebjoo ◽  
M. Hedayati ◽  
R. Hoshyar ◽  
C.-J. Huang
Keyword(s):  
Breast Cancer ◽  
Aqueous Extract ◽  
Protein Level ◽  
High Intensity ◽  
Caspase 3 ◽  
Tumor Tissue ◽  
Tumor Volume ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
High Intensity Interval

AbstractThis study was to investigate whether high-intensity interval training (HIIT) and saffron aqueous extract (SAE) would provide a synergistic effect to improve tumor volume reduction and also modulate pro- and anti-apoptotic protein expression in tumor tissue of 4T1 breast cancer-bearing mice. Female mice following induction of breast cancer through injection of 4T1 cell lines were randomly divided into four groups: (1) HIIT, (2) SAE, (3) HIIT+ SAE, and (4) control. The tumor volume was significantly lower in the HIIT, SAE, and HIIT+SAE groups than in the controls. The protein level of caspase-3 in the HIIT and the SAE groups was higher than in the control and the HIIT+SAE groups. The Bax protein level in the SAE group was higher than in the control. The HIIT+SAE group showed a lower level of Bax than the HIIT and the SAE groups. The protein level of Bcl-2 was higher in the HIIT+SAE vs. both the HIIT and the SAE groups. Finally, the ratio of Bax/Bcl-2 was significantly higher in the HIIT and the SAE groups than in the HIIT+SAE and control groups. These findings indicate that a combination of HIIT and SAE interventions does not improve the apoptotic induction in tumor tissue, while both HIIT and SAE treatments may mediate apoptotic pathway as evinced by the elevated ratio of Bax/Bcl-2 and caspase-3 levels during tumor progression in breast cancer-bearing mice.

scholarly journals High intensity exercise during breast cancer chemotherapy - effects on long-term myocardial damage and physical capacity - data from the OptiTrain RCT

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Josefin Ansund ◽  
Sara Mijwel ◽  
Kate A. Bolam ◽  
Renske Altena ◽  
Yvonne Wengström ◽  
...  
Keyword(s):  
Breast Cancer ◽  
High Intensity ◽  
Troponin T ◽  
Interval Training ◽  
High Intensity Exercise ◽  
Moderate Intensity ◽  
High Intensity Interval Training ◽  
Post Intervention ◽  
High Intensity Interval

Abstract Background Adjuvant systemic breast cancer treatment improves disease specific outcomes, but also presents with cardiac toxicity. In this post-hoc exploratory analysis of the OptiTrain trial, the effects of exercise on cardiotoxicity were monitored by assessing fitness and biomarkers over the intervention and into survivorship. Methods; Women starting chemotherapy were randomized to 16-weeks of resistance and high-intensity interval training (RT-HIIT), moderate-intensity aerobic and high-intensity interval training (AT–HIIT), or usual care (UC). Outcome measures included plasma troponin-T (cTnT), Nt-pro-BNP and peak oxygen uptake (VO2peak), assessed at baseline, post-intervention, and at 1- and 2-years. Results For this per-protocol analysis, 88 women met criteria for inclusion. Plasma cTnT increased in all groups post-intervention. At the 1-year follow-up, Nt-pro-BNP was lower in the exercise groups compared to UC. At 2-years there was a drop in VO2peak for patients with high cTnT and Nt-pro-BNP. Fewer patients in the RT-HIIT group fulfilled biomarker risk criteria compared to UC (OR 0.200; 95% CI = 0.055–0.734). Conclusions In this cohort, high-intensity exercise was associated with lower levels of NT-proBNP 1-year post-baseline, but not with cTnT directly after treatment completion. This may, together with the preserved VO2peak in patients with low levels of biomarkers, indicate a long-term cardioprotective effect of exercise. Trial registration Clinicaltrials.govNCT02522260, Registered 13th of august 2015 – Retrospectively Registered Graphical abstract

Sign in / Sign up

Export Citation Format

Share Document