scholarly journals Pengaruh Lama Perendaman Kolkisin terhadap Pertumbuhan Planlet (Chrysanthemum morifolim R) Krisan Varietas Pasopati Cara In Vitro

2018 ◽  
Vol 6 (2) ◽  
pp. 124
Author(s):  
Arip Nursalmin ◽  
Ai Komariah ◽  
Odang Hidayat
Keyword(s):  
Root Length ◽  
Environmental Design ◽  
Plant Biotechnology ◽  
Root Number ◽  
Random Design ◽  
Number Of Leaves ◽  
The University ◽  
Laboratory Time

Laboratory-time studies to determine the duration of immersion of colchicine against growth of planlet (Krrysanthemum morifolim R) chrysanthemum varieties of in vitro way inpatients. The experiment was carried out at the Plant Biotechnology Laboratory of the Faculty of Agriculture of the University of Winaya Mukti from July to September 2017. The environmental design using Randomized Completely Random Design (RAL) consists of six treatments with 4 replications including: A (without colchicine immersion), B (immersion colcisin 1 hour, C (2-hour colchicine immersion), D (3-hour colchicine immersion), E (4-hour colchicine immersion) and F (5-hour colchicine immersion). The results showed that the duration of immersion of kolkisin on chrysanthemum varieties of pasopati in vitro gave a significant effect on the observation of the number of leaves aged 2 MST, 4 MST, 6 MST and 8 MST, the number of books aged 4 MST, 6 MST and 8 MST, root length , the number of roots as well as the height of the planlet. Immersion of 1 hour colchicine had a better effect on the observation of leaf count of age 2 MST, 4 MST, 6 MST and 8 MST, number of books aged 4 MST, 6 MST and 8 MST, root length, root number and plantlet height.

scholarly journals The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro

2018 ◽  
Vol 6 (2) ◽  
pp. 23
Author(s):  
Arisah Hasanah ◽  
Fauziyah Harahap ◽  
Ramlan Silaban
Keyword(s):  
Root Length ◽  
Leaf Length ◽  
Ananas Comosus ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Shoot Height ◽  
Leaf Emergence ◽  
Two Factors ◽  
Number Of Leaves

Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar. 

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals PENGARUH KONSENTRASI ASAM GIBERELAT (GA ) DAN LAMA PERENDAMAN TERHADAP VIABILITAS, VIGOR dan 3 PERTUMBUHAN BENIH JARAK (Jatropha curcas L.) KLON IP-1P di PEMBENIHAN

2017 ◽  
Vol 3 (2) ◽  
pp. 9
Author(s):  
Asep Samsul Mustopa
Keyword(s):  
Block Design ◽  
Seed Viability ◽  
Plant Biotechnology ◽  
Seed Vigor ◽  
Growth And Yield ◽  
Root Number ◽  
Soaking Time ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves

The experiment was carried out at Plant Biotechnology laboratorium and experimentalfield Faculty of Agriculture University of Winaya Mukti Tanjungsari Sumedang which the altitude is about 850 meters above sea level, from March 2008 until May 2008.Theobjective of this experiment was to studying the interaction effect of giberellat acidconcentration and and soaking time on seed viability, seed vigor, growth and yield ofJatropha curcas in nursery.Design used was Randomized Block Design (RBD) withfactorial pattern, treatment consisted of two factors and two replications. The first factorwas giberellat acid (GA3) concentration (K) which consisted of 4 levels namely : k1=250 ppm, k2= 500 ppm, k3= 750 ppm and k4= 1000 ppm. The second factor soakingtime on GA34 (M) which consisted of 4 levels namely : m1= 6 hour, m2= 18 hour dan m = 24 hour.The results of these experiment showed there was aninteraction between giberellat acid concentration and and soaking time on high of plant20 day after planting (DAP), 40 DAP, 60 DAP, number of leaves 20 DAP, 40 DAP, 60DAP, length of root, number of root, dry wight of root and dry wight of shoot. 

scholarly journals In vitro callus initiation and regeneration of potato

1970 ◽  
Vol 34 (3) ◽  
pp. 449-456 ◽  
Author(s):  
AU Haque ◽  
MA Ehsanul Samad ◽  
TL Shapla
Keyword(s):  
Key Words ◽  
Root Length ◽  
Shoot Length ◽  
Leaf Explant ◽  
Callus Initiation ◽  
Number Of Leaves ◽  
Interaction Term ◽  
Shoot Tip Explants ◽  
Internode Explants

The first experiment involving different explants and concentrations of 2,4-D and kinetin showed highly significant differences for length and weight of callus formed except interaction of callus weight. Leaf explant appeared to be best of all for callus length and weight when 1.0 mg/L 2,4-D + 0.25 mg/L kinetin concentration was used. Similarly, different explants versus different concentrations of BAP/GA3/IAA showed significant differences for shoot length and leaf number per plantlet and also for root length. However, interaction term confirmed node and node/internode explants produced better results in shoot length and number of leaves per plantlet when concentrations 1 .0 mg/L BAP + 0.1 mg/L GA3 and 1.0 mg/L BAP + 0.2 mg/L GA3, 1.0 mg/L BAP + 0.4 mg/L GA3, respectively, were used. Similarly, internode explants produced better results for root length after 21 days plantlet-1 when concentration of 1.0 mg/L IAA + 0.25 mg/L GA3 was used. Shoot tip explants also produced better results in root length after 28 days plantlet-1 when concentrations 1.0 mg/L IAA + 0.25 mg/L GA3 were used. Key Words: In vitro, callus initiation, regeneration, potato. DOI: 10.3329/bjar.v34i3.3971 Bangladesh J. Agril. Res. 34(3) : 449-456, September 2009

scholarly journals Effect of some botanical fungicide and chemicals as forest seed dressing  on the quality of the seeds: تأثير بعض مبيدات الفطريات والكيمياويات النباتية في تلبيس بذور الغابات  على جودة البذور

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Shler Hassan Mahmood, Jalal Hama Salih Ismael
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Germination Percentage ◽  
Shoot Length ◽  
Salix Alba ◽  
Seed Dressing ◽  
Number Of Leaves ◽  
Petri Plate ◽  
Local Plants ◽  
The University

An experiment was conducted to study the antifungal activity of different concentrations aqueous extracts from three local plants, Hypericum triquetrifolium, Salix alba, Salvia rosmarinus and also different concentrations of fungicide (Captan) and Salicylic acid were evaluated for their efficacy as seed dressing on Catalpa and Albizia that seeds were soaked separately for 30 mints in various concentrations of aqueous extract and fungicide. Forest seeds were grown in pots which were inoculated by Sclerotinia sclerotiorum and Fusarium oxysporum that one petri plate/ pot in 2018 in a greenhouse at the University of Sulaimani–College of Agricultural Sciences Engineering. The results showed that seed germination percentage, shoot length, root length and number of leaves was significantly (p≤0.05) increased with both the treatments. Captan (0.5%) was more effective in comparison with Captan (0.3%) and seed germination, shoot length, root length and number of leaves was gradually increased with increase of the doses of fungicides. It can be suggested that fungicide Captan have antifungal effect and can be used to treat seed against seed borne pathogens of forest, increase seed germination and shoot length, root length and number of leaves.

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

scholarly journals The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

2017 ◽  
Vol 21 (2) ◽  
pp. 109 ◽  
Author(s):  
Aries Bagus Sasongko ◽  
Asruwaidah Fatumi ◽  
Ari Indrianto
Keyword(s):  
Carbon Dioxide ◽  
Root Length ◽  
Unit Area ◽  
Leaf Length ◽  
Growth Increase ◽  
Photoautotrophic Micropropagation ◽  
Average Quantity ◽  
Chlorophyll A And B ◽  
Number Of Leaves

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

scholarly journals Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

2018 ◽  
Vol 4 (2) ◽  
pp. 93
Author(s):  
Douglas Bertoncelli ◽  
Guilherme Alves ◽  
Gustavo Freiria ◽  
Felipe Furlan ◽  
Helio Neto ◽  
...  
Keyword(s):  
Root Length ◽  
Iron Concentration ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Nutritional Requirement ◽  
Initial Development ◽  
Shoot Height ◽  
Shoot Number ◽  
Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

scholarly journals Effects of Growth Regulators and Type-Variety of Oil Palm (Elaeis guineensis Jacq.) on Direct Organogenesis

2018 ◽  
Vol 10 (2) ◽  
pp. 251-258
Author(s):  
Chibuzo Nwabueze Chikezie NWAOGUALA ◽  
Hakeem Olalekan SHITTU
Keyword(s):  
Growth Regulators ◽  
Oil Palm ◽  
Root Length ◽  
Elaeis Guineensis ◽  
Root Production ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
Elaeis Guineensis Jacq

The in vitro organogenesis of embryos of type-variety (‘Albescens’, ‘Nigrescens’, ‘Virescens’ and ‘Idolatrica’) of the oil palm (Elaeis guineensis Jacq.) with growth regulators was studied. Murashige and Skoog (MS) medium, supplemented with nine treatment concentrations of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP), alone and in combinations, was used to initiate embryo cultures of the type-variety. Data were collected on time of root production, root length, plant height, number of leaves and number of roots.  The results obtained indicated that there were swelling and expansion of the matured zygotic explants in cultures within 7 and 14 days after culture initiation (DCI). Time of root production did not vary significantly among type-variety, but differed significantly (p < 0.001) due to growth regulators, whereas their interaction effect was non-significant. Root length and plantlet height significantly varied (p < 0.05) due to type-variety and growth regulators, as well as their interaction. There were no significant variations among type-variety on number of leaves and roots produced at 84 DCI, but there was significant (p < 0.05) variations on number of leaves and non-significant variance on number of roots due to growth regulators. The type-variety combined with growth regulators effects on number of leaves and roots were not significant.  In the present study, the responses of the various type-variety in the regeneration of plantlets in vitro varied with respect to media supplementation and that low concentration of NAA and BAP, alone and in different combinations, favoured root, leaf and plantlet production in MS medium.

scholarly journals Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

2017 ◽  
Vol 52 (11) ◽  
pp. 1123-1126 ◽  
Author(s):  
Filipe Almendagna Rodrigues ◽  
Renata Alves Lara Silva Rezende ◽  
Moacir Pasqual ◽  
Maria Teresa Gomes Lopes
Keyword(s):  
In Vitro Culture ◽  
Activated Charcoal ◽  
Root Length ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Fresh Matter ◽  
Number Of Leaves

Abstract: The objective of this work was to evaluate the effects of the solidifying agents agar and phytagel and of activated charcoal on the in vitro cultivation of two maná cubiu (Solanum sessiliflorum) varieties: Thaís and Santa Luzia. The phytotechnical characteristics analyzed included number of leaves, number of roots, shoot and root length, and fresh matter of shoot and root. Regardless of the variety, phytagel was superior to agar as a culture medium. A greater number of leaves and longer shoots were observed in the Santa Luzia variety, in the absence of charcoal. The Thaís variety showed longer shoots and roots in the presence of charcoal.

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