The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.)  from Sipahutar North Sumatera In Vitro

Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar.

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Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

Agronomy Science and Biotechnology ◽

10.33158/asb.2018v4i2p93 ◽

2018 ◽

Vol 4 (2) ◽

pp. 93

Author(s):

Douglas Bertoncelli ◽

Guilherme Alves ◽

Gustavo Freiria ◽

Felipe Furlan ◽

Helio Neto ◽

...

Keyword(s):

Root Length ◽

Iron Concentration ◽

Leaf Length ◽

In Vitro Cultivation ◽

Nutritional Requirement ◽

Initial Development ◽

Shoot Height ◽

Shoot Number ◽

Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

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Keragaan tiga jenis planlet anggrek Phalaenopsis asal Protocorm yang diinduksi Ethyl Methyl Sulfonate (EMS) secara in vitro

Kultivasi ◽

10.24198/kultivasi.v17i1.16077 ◽

2018 ◽

Vol 17 (1) ◽

Author(s):

Romiyadi Romiyadi ◽

Ai Komariah ◽

Suseno Amien

Keyword(s):

Root Length ◽

Variable Number ◽

Leaf Length ◽

Ethyl Methyl ◽

Randomized Design ◽

Methyl Sulfonate ◽

Two Factors ◽

Number Of Leaves ◽

Ethyl Methyl Sulfonate

Abstract. This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and v3 (Phalaenopsis Luchia Pink X Phalae-nopsis Chain Xen Mammon). The second factor was the concentration of EMS that consisted of e0 (0% EMS/control), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), and e5 (0,25% EMS). Explant protocorm of three types of Phalae-nopsis soaked in a solution of EMS by each treat-ment for 3 hours, and cultured on MS medium Basal Modified Multiplication Shoot for 10 weeks. The experimental results showed that there are interaction between the three types of Phalae-nopsis result of a cross with a concentration of EMS to variable number of roots. Orchids P. 717 X P. Fire Bird had higher number of leaves, number of roots, leaf leang, and root length than the other. The result showed that there were interaction between three species of Phalaenopsis orchid from the crossing with EMS concentration on root variables. Orchid P. 717 X P. Fire Bird has the number of leaves, the number of roots, the number of shoots, leaf length and root length better than other types. EMS concentrations independently at all levels of treatment can not increase the number of leaves, the number of roots, fresh weight of planlet, leaf length, and root length.Keywords: Phalaenopsis, the concentration of EMS, in vitro cultureSari. Penelitian ini mempelajari dan mengetahui pengaruh konsentrasi Ethyl Methyl Sulfonate (EMS) terhadap keragaan planlet tiga jenis anggrek Phalaenopsis asal protocorm dan mencari konsentrasi optimum EMS untuk setiap jenis anggrek Phalae-nopsis secara in vitro.Percobaan dilaksanakan di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Winaya Mukti Sumedang. Eksperimen menggunakan Rancangan Acak Lengkap (RAL) Pola Faktorial, yang terdiri atas dua faktor dan diulang sebanyak dua kali.Faktor pertama adalah jenis anggrek Phalaenopsis hasil persilangan yang terdiri atas tiga jenis, yaitu v1 (Phalaenopsis 717 X Phalaenopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), dan v3 (Phalaenopsis Luchia Pink X Phalaenopsis Chain Xen Mammon). Faktor kedua adalah EMS yang terdiri atas enam taraf perlakuan, yaitu e0 (0% EMS/kontrol), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), dan e5 (0,25% EMS). Eksplan berupa protocorm dari tiga jenis anggrek Phalaenopsis hasil persilangan yang direndam dalam larutan EMS berdasarkan masing-masing perlakuan selama 3 jam, dan dikulturkan pada media MS Modified Multiplication Shoot Basal selama 10 minggu.Hasil penelitian menunjukkan terjadi interaksi antara tiga jenis anggrek Phalaenopsis hasil persilangan dengan konsentrasi EMS terhadap variabel jumlah akar.Anggrek P. 717 X P.Fire Bird memiliki jumlah daun, jumlah akar, jumlah tunas, panjang daun dan panjang akar yang lebih baik dibandingkan jenis lainnya. Konsentrasi EMS secara mandiri pada semua taraf perlakuan tidak dapat meningkatkan jumlah daun, jumlah akar, bobot segar planlet, panjang daun, dan panjang akar.Kata Kunci: Phalaenopsis, Konsentrasi EMS, dan Budidaya In Vitro

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Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Caraka Tani Journal of Sustainable Agriculture ◽

10.20961/carakatani.v35i1.27756 ◽

2019 ◽

Vol 35 (1) ◽

pp. 23

Author(s):

Siti Mardhikasari ◽

Ahmad Yunus ◽

Samanhudi Samanhudi

Keyword(s):

Root Length ◽

Leaf Length ◽

Shoot Length ◽

Coconut Water ◽

Root Number ◽

Foliar Fertilizer ◽

Two Factors ◽

The Media ◽

Four Levels

The use of foliar fertilizers and coconut water is potential; foliar fertilizer is the alternative medium and coconut water is the plant growth regulator (PGR) in the banana tissue culture. This study aimed to examine the ability of foliar fertilizer to substitute Murashige and Skoog (MS) media and coconut water as the main source of cytokine. There are two factors in this research. The first factor is the media, which consist of three levels, i.e. fully MS, ½ MS + ½ foliar fertilizer and fully foliar fertilizer. The second factor is the coconut water with four levels of concentration i.e. 50, 100, 150 and 200 ml l<sup>-1</sup>. The results show that fully formulated foliar fertilizer had not been able to substitute or even compete with a fully MS media, but conversely foliar fertilizer was used to substitute ½ MS media. This can be seen from the parameters of shoot length, leaf length, root number and root length with the highest yield that was found in MS media (8.7, 4.66, 3.33 and 3.23 cm). The 50-100 ml l<sup>-1 </sup>concentrations of coconut water showed the best results by giving a significant effect on the number of roots and root length. The acclimatization of plantlets in this study was nearly 100%, indicated by the formation of a complete organ.

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The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.27167 ◽

2017 ◽

Vol 21 (2) ◽

pp. 109 ◽

Cited By ~ 1

Author(s):

Aries Bagus Sasongko ◽

Asruwaidah Fatumi ◽

Ari Indrianto

Keyword(s):

Carbon Dioxide ◽

Root Length ◽

Unit Area ◽

Leaf Length ◽

Growth Increase ◽

Photoautotrophic Micropropagation ◽

Average Quantity ◽

Chlorophyll A And B ◽

Number Of Leaves

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

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Effects of Nutrient Sources on the Early Growth of Pineapple Plantlets (Ananas comosus (L) Merr) in the Nursery

Journal of Fruit and Ornamental Plant Research ◽

10.2478/v10290-012-0013-9 ◽

2012 ◽

Vol 20 (2) ◽

pp. 35-40

Author(s):

Solomon Olusegun Omotoso ◽

Ezekiel Akinkunmi Akinrinde

Keyword(s):

Root Length ◽

Poultry Manure ◽

Early Growth ◽

Commercial Production ◽

Leaf Length ◽

Ananas Comosus ◽

Mineral Fertilization ◽

Nutrient Sources ◽

Inadequate Information ◽

Number Of Leaves

Abstract Inadequate information on the nutrition of pineapple limits its commercial production in Nigeria. The effects of poultry manure and urea (each at 50 and 100 kg N ha-1), as well as their 1:1 (organo-mineral) combinations at 50, 100, 150 and 200 kg N ha-1, on the early (nursery) growth of pineapple plantlets. Organo-mineral fertilization at 100 kg N ha--1 resulted in the highest D-leaf length, number of leaves, root length and leaf area, while the highest biomass production (approx. 304 g, just within the 300-400 g desirable range for transplanting to the field) was achieved at 150 kg N ha-1.

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THE GROWTH OF “PISANG RAJA BULU” PLANLETS IN THE LIGHTING OF INCUBATION ROOMS AND GROWTH REGULATOR AGENTS OF BROWNING PREVENTION BY IN VITRO

Agrivet ◽

10.31315/agrivet.v25i1.4170 ◽

2019 ◽

Vol 25 (1) ◽

pp. 8

Author(s):

Utami Setyawati ◽

Ari Wijayani ◽

Endah Wahyurini

Keyword(s):

Vitamin C ◽

Root Length ◽

Fresh Weight ◽

Experiment Method ◽

Two Factors ◽

Main Plot ◽

Number Of Leaves ◽

Plot Design ◽

Number Of Shoots

The aims of this research were to determine the lighting of incubation rooms and browning prevention agents on the growth of “Pisang Raja Bulu” planlets by in vitro and determine whether there is a best interaction between the incubation rooms lighting and the type of browning prevention agents in the growth of “Pisang Raja Bulu” planlets that plants in vitro. This research was conducted in the Biotechnology laboratory of the Faculty of Agriculture, UPN "Veteran" Yogyakarta on June - August 2018. The research method was a laboratory experiment method compiled with the Split Plot Design of two factors. The first factor as a main plot is the incubation room lighting, which is with light for 90 days, without light for 90 days, without light in the first 45 days, and without light in the last 45 days. The second factor as a sub plot is browning prevention agents, named thidiazuron, activated charcoal, and vitamin C. Each combination of treatments was repeated 3 times. The result showed that there was the best combinations of treatments that is all combination of lighting and vitamin C 0,88 mg / l in terms of browning. The first 45 days lighting treatment gave the best result on the percentage of life, plantlet height, number of shoots, number of leaves, root length, and fresh weight. The treatment of vitamin C 0.88 mg / l gave the best result on the percentage of life, plantlet height, number of shoots, root length, number of roots, fresh weight, and dry weight.Keyword: in vitro, lighting, browning, raja bulu banana.

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Pengaruh Lama Perendaman Kolkisin terhadap Pertumbuhan Planlet (Chrysanthemum morifolim R) Krisan Varietas Pasopati Cara In Vitro

Paspalum: Jurnal Ilmiah Pertanian ◽

10.35138/paspalum.v6i2.95 ◽

2018 ◽

Vol 6 (2) ◽

pp. 124

Author(s):

Arip Nursalmin ◽

Ai Komariah ◽

Odang Hidayat

Keyword(s):

Root Length ◽

Environmental Design ◽

Plant Biotechnology ◽

Root Number ◽

Random Design ◽

Number Of Leaves ◽

The University ◽

Laboratory Time

Laboratory-time studies to determine the duration of immersion of colchicine against growth of planlet (Krrysanthemum morifolim R) chrysanthemum varieties of in vitro way inpatients. The experiment was carried out at the Plant Biotechnology Laboratory of the Faculty of Agriculture of the University of Winaya Mukti from July to September 2017. The environmental design using Randomized Completely Random Design (RAL) consists of six treatments with 4 replications including: A (without colchicine immersion), B (immersion colcisin 1 hour, C (2-hour colchicine immersion), D (3-hour colchicine immersion), E (4-hour colchicine immersion) and F (5-hour colchicine immersion). The results showed that the duration of immersion of kolkisin on chrysanthemum varieties of pasopati in vitro gave a significant effect on the observation of the number of leaves aged 2 MST, 4 MST, 6 MST and 8 MST, the number of books aged 4 MST, 6 MST and 8 MST, root length , the number of roots as well as the height of the planlet. Immersion of 1 hour colchicine had a better effect on the observation of leaf count of age 2 MST, 4 MST, 6 MST and 8 MST, number of books aged 4 MST, 6 MST and 8 MST, root length, root number and plantlet height.

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Effect of Cow Urine and IBA on the Growth of Sweet Leaf (Sauropus androgynus (L.) Merr.) Cuttings

JURNAL AGRONIDA ◽

10.30997/jag.v5i1.1854 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Fawziyah Nurshabrina ◽

Arifah Rahayu ◽

Oktavianus Lt

Keyword(s):

Growth Regulators ◽

Plant Roots ◽

Indole Butyric Acid ◽

Rooted Cuttings ◽

Shoot Height ◽

Cow Urine ◽

Two Factors ◽

Number Of Leaves ◽

Leaf Vegetable ◽

Number Of Shoots

Sweet leaf (Sauropus androgynus (L.) Merr.), a leaf vegetable that is generally propagated vegetatively with cuttings. This study aims to determine the effect of accession and administration of natural and synthetic auxin growth regulators on the growth of sweet leaf cuttings. This study uses factorial Randomized Complete Design consisting of two factors, namely accession (Banten from Pandeglang, Bogor from Tajur, and Cianjur from Cugenang) and auksin combination (IBA 100%R, urine 25%R + IBA 75%R, urine 50%R + IBA 50%R, urine 75%R + IBA 25%R, and urine 100%R). The results showed that the growth of Cianjur accession had the best results on the percentage of growth cuttings, the percentage of shoot buds, shoot height, number of shoots, number of leaves, percentage of rooted cuttings, and number of roots compared to Banten and Bogor accessions. Giving urine 100%R significantly increases plant height, shoot height, number of shoots, and number of sweet leaf plant roots compared with other auxin combinations. The combination treatment of auxin and accession showed results that were not significantly different from the variable root length and weight of the harvest. In sweet leaf plants, cow urine can replace the use of IBA (Indole Butyric Acid). Keywords: Sauropus androgynus, vegetative, urine, IBA

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KOMBINASI MEDIA TANAM DAN PEMBERIAN BERBAGAI DOSIS PUPUK GROW QUICK LB TERHADAP PERTUMBUHAN ANGGREK DENDROBIUM (Dendrobium sp.) PASCA AKLIMATISASI

Photon: Jurnal Sain dan Kesehatan ◽

10.37859/jp.v8i01.535 ◽

1930 ◽

Vol 8 (01) ◽

pp. 91-97

Author(s):

Nofripa Herlina ◽

Novia Gesriantuti ◽

Anis Restiawati

Keyword(s):

Economic Value ◽

Leaf Length ◽

Leaf Width ◽

Growing Media ◽

Randomized Design ◽

Two Factors ◽

The Media ◽

Number Of Leaves ◽

Medium Type

Dendrobium orchid is one of the very popular ornamental plant because of the flower is beautiful and has a high economic value. An effort to prevent the orchid from extinetion is by means of in vitro propagation. The study aims to look at the effect of various doses of fertilizers Grow Quick LB and types of growing media and their interaction on the growth of Dendrobium sp. post acclimatization.The completely randomized design was used with two factors. The firs factor was type medium (charcoal+fern, charcoal+cocopeat), the second factor was type of Grow Quick LB fertilizer (0ml/L, 1ml/L, 2ml/L, 3ml/L). Parameters observed were, plantlet height, leaf length, leaf width, and number of leaves. The results showed that the dosing of Grow Quick LB fertilizer 1ml/L showed the highest values for the parameters width of leaves and number of leaves, whereas a dose of 2ml/L showed the highest values for the parameters plantlet height, leaf length. A combination of types of growing media charcoal+cocopeat showed a better effect than the media charcoal+fern on all parameters. The interaction of medium type and Grow Quick LB fertilizer significantly different with plantlet height and leaf length and not significantly different with parameters leaf width, and number of leaves.

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The shoot explant types effect of eleven stevia (Stevia rebaudiana Bertoni)

Jurnal Agro ◽

10.15575/13367 ◽

2021 ◽

Vol 8 (1) ◽

pp. 142-152

Author(s):

Suseno Amien ◽

Arini Zahra Azhari ◽

Citra Bakti ◽

Haris Maulana

Keyword(s):

Stevia Rebaudiana ◽

Shoot Tip ◽

Shoot Height ◽

Stevia Rebaudiana Bertoni ◽

Randomized Design ◽

Two Factors ◽

Wet Weight ◽

Number Of Leaves ◽

Number Of Shoots

Explants play an important role in thepropagation system of stevia (Stevia rebaudiana Bertoni). A Completely Randomized Design (CRD) was used in this experiment with factorial pattern consisting of two factors, namely three types of explants (shoot tip, first node, and second node) and eleven accessions of stevia namely a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). The results showed that shoot tip explant was the best explant than first node and second node for the number of shoots (18.11 shoots), number of leaves (93.49 leaves) and wet weight (3.56 grams). The best accession of shoot height was a10(SGR 7.5) (19.95 cm), the highest number of shoots wasa7(SBG 10) (21.87 shoots), the highest number of leaves wasa7(SBG 10) (138.00 leaves), the heaviest wet weight wasa7(SBG 10) (3.56 grams), the highest leaf chlorophyll content was a10 (SGR 7.5) (0.63 µg mL-1). Accessions with the fastest root initiation time at the rooting stage was a11 (TR 3.5) (4.00 DAC), the highest number of roots wasa10 (TR 3.5) (27.11 roots), the best root length wasa2(Garut) (4.51 cm). Information on the best explant types and stevia accessions in the in-vitro multiplication stage can be used as the basis for stevia breeding programs in Indonesia.AbstractEksplan berperan penting dalam sistem perbanyakan Stevia (Stevia rebaudiana Bertoni). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu tiga jenis eksplan (ujung pucuk, ruas pertama, dan ruas kedua) dan sebelas aksesi stevia yaitu a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). Hasil penelitian menunjukkan bahwa eksplan ujung pucuk merupakan eksplan terbaik dibandingkan buku pertama dan kedua untuk jumlah tunas (18,11 tunas), jumlah daun (93,49 daun) dan berat basah (3,56 gram). Aksesi terbaik pada tinggi pucuk adalah a10(SGR 7.5) (19,95 cm), jumlah pucuk tertinggi a7 (SBG 10) (21,87 pucuk), jumlah daun terbanyak a7 (SBG 10) (138.00 helai daun), bobot basah terberat a7 (SBG 10) (3,56 gram), kandungan klorofil daun tertinggi adalah GR 7,5 (0,63 µg mL-1). Aksesi dengan waktu inisiasi akar tercepat pada tahap perakaran adalah TR 3,5 (4,00 HST), jumlah akar terbanyak adalah TR 3,5 (27,11 akar), panjang akar terbaik adalah a2(Garut) (4,51 cm). Informasi jenis eksplan dan aksesi stevia terbaik pada tahap multiplikasi in-vitro dapat dijadikan dasar program pemuliaan stevia di Indonesia.

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