Assessment of lead toxicity in red tilapia Oreochromis sp. through hematological parameters

Lead exposure is associated with several health hazards among workers with different individual responses. We conducted this study to determine the possible effects of lead exposure on hematological parameters and kidney function of a group of Egyptian ammunition workers and the interaction of aminolevulinic acid dehydratase (ALAD) G177C gene polymorphisms as an effect modifier. Significant differences were observed between exposed workers with ALAD1-1 and ALAD1-2 genotypes in terms of blood lead level, hematological parameters and kidney function. It seems that δ-ALAD gene polymorphism may be an effect modifier and a marker of genetic susceptibility to lead toxicity.

Download Full-text

Effects of chemical silage of red tilapia viscera (Oreochromis spp.) as a source of protein on the productive and hematological parameters in isa-brown laying hens (Gallus gallus domesticus)

Heliyon ◽

10.1016/j.heliyon.2020.e05831 ◽

2020 ◽

Vol 6 (12) ◽

pp. e05831

Author(s):

Yhoan S. Gaviria G ◽

Luis F. Londoño F ◽

José E. Zapata M

Keyword(s):

Laying Hens ◽

Hematological Parameters ◽

Gallus Gallus ◽

Gallus Domesticus ◽

Gallus Gallus Domesticus ◽

Red Tilapia

Download Full-text

A study on the ALAD gene polymorphisms associated with lead exposure

Toxicology and Industrial Health ◽

10.1177/0748233708095770 ◽

2008 ◽

Vol 24 (7) ◽

pp. 501-506 ◽

Cited By ~ 26

Author(s):

AP Shaik ◽

K Jamil

Keyword(s):

Lead Exposure ◽

Aminolevulinic Acid ◽

White Cell Count ◽

Hematological Parameters ◽

Lead Toxicity ◽

Blood Lead ◽

Stripping Voltammetry ◽

Genotype Group ◽

Significant Difference ◽

Occupationally Exposed

δ-aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning, and polymorphisms in this gene may affect the response of individuals to lead toxicity symptoms. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on blood lead levels (BLL) and hematological parameters. In all, 113 battery manufacturing unit workers and 102 occupationally unexposed controls from Hyderabad, A.P, India formed the study group. Genotypes for the ALAD G177C polymorphism were determined by Polymerase chain reaction and restriction fragment length digestion. BLL were determined by anode stripping voltammetry using ESA Model 3010B Lead analyzer. Complete blood picture was analyzed using ADVIA cell counter for each sample. The frequencies for ALAD1 and ALAD2 alleles were 0.98 and 0.01, respectively. ALAD 1-2 and ALAD 2-2 genotypes together were considered as a single group and compared with the ALAD 1-1 genotype group. BLL did not differ significantly among ALAD1-1, 1-2, and 2-2 genotypes; however, subjects from the ALAD 1-2/2-2 genotype group showed higher BLL concentrations of 80.51 μg/dL when compared with subjects from the ALAD 1-1 genotype group (50.4 μg/dL). Approximately 29.2% volunteers ( n = 33) from the occupationally exposed group had hemoglobin levels below 10.0 g/dL. There was no significant difference in total white cell count and platelet count between occupational and non-exposed lead-exposed groups. BLL of occupationally exposed individuals were significantly high compared with the unexposed group. ALAD G177C polymorphism along with BLL and assessment of hematological parameters may play an important role in evaluation and better understanding of the consequences of lead exposure.

Download Full-text

Lead acetate induced toxicities and antitoxic effect of Vitamin E and selenium in mice

10.33109/bjvmjj2021lam1 ◽

2021 ◽

Vol 19 (1) ◽

pp. 73-83

Author(s):

M. S. Jahan ◽

M. S. Islam ◽

M. Gautam ◽

M. E. R. Bhuiyan

Keyword(s):

Vitamin E ◽

Lead Acetate ◽

Hematological Parameters ◽

Lead Toxicity ◽

Treated Group ◽

The Body ◽

Premature Delivery ◽

Dependent Manner ◽

Female Mice ◽

Dose Dependent

Background: The experiment was conducted to evaluate the effect of different dosages of lead in the hematological parameters of mice and to observe the antitoxic role of Vitamin E and Selenium in induced lead toxicities. Moreover, the toxic effect of lead in the reproduction of female mice was also examined. Methods: A total of 72 (48 male and 24 female) Swiss albino mice were used in the experiment. After adaptation, 42 male mice were selected for hematological studies and divided into seven groups (n=6) where Group A represented healthy control mice and Group B, C, and D were treated with lead acetate at the rate of 0.5mg/kg, 1mg/kg and 2mg/kg respectively. Similarly, three other groups B+, C+, and D+ were treated with lead acetate plus Vitamin E and Selenium at the rate of 2ml per liter drinking water. A total of 24 female mice were divided into four groups (n=6), group E represented control mice and Group F, G, and H were treated with lead acetate at the rate of 0.5mg/kg, 1mg/kg, and 2mg/kg for three weeks followed by matting and treatment was continued for another one week of gestation. Blood sample was analyzed from the hematological study group. Result: The lead treatment caused a dose-dependent decrease in the value of Hb and PCV significantly whereas the value of TEC and TLC were significantly decreased in Group C and D in relation to Control. The value of ESR increased significantly in a dose-dependent manner in Group D in relation to Control whereas MCV and MCH values were significantly decreased than that of control. The value of TEC, Hb, PCV, ESR, and TLC improved in the Lead plus Vitamin E- Selenium treated group as compared to the Lead treated group but, only Group C+ showed significant improvement as compared to Group C. The value of neutrophil and monocyte were significantly decreased were as lymphocyte and eosinophil were significantly increased relative to control. There was a dose-dependent effect of lead in pregnancy of female mice with the highest effect (premature delivery and infant mortality) on high dose treated mice. Conclusion: It can be concluded that lead has a great impact on hematological parameters and has an effect on various systems of the body. Premature birth and abortion are major effects of lead toxicity. Our results suggest that hemolysis of RBC and or impairment of erythropoiesis may be caused by lead toxicity and the hematological values can be restored by the use of Vitamin E plus Selenium.

Download Full-text

Effect of Different Feed Restrictions on Growth, Biometric, and Hematological Response of Juvenile Red Tilapia (Oreochromis spp)

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v13i2.26302 ◽

2021 ◽

Vol 13 (2) ◽

pp. 243

Author(s):

Adam Robisalmi ◽

Kartiawati Alipin ◽

Bambang Gunadi

Keyword(s):

Blood Glucose ◽

Growth Condition ◽

Hematological Parameters ◽

Feed Restriction ◽

Hepatosomatic Index ◽

Red Tilapia ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Stress Levels ◽

The Impact

Highlight ResearchThere has been a significant decrease in growth, condition factors, hepatosomatic index along viscerosomatic index with the length of time for feed restriction, although fasting for up to 28 days did not cause fish mortalityHematological parameters such as RBCs, WBCs, hematocrit, and hemoglobin decreased significantly, but after re-feeding increased significantly in consecutive timesThe blood glucose levels decreased during feed restriction and gradually increased after re-feedingFeed restriction ﬁsh gained weight continuously during re-feeding, but no displaying compensatory growthThe decrease in hematological parameters did not indicate stress levels in fish, but reflected a lack of nutrition condition AbstractThe management of red tilapia culture can be improved through restriction of feed. This study aimed to determine the impact of feed restriction time and re-feeding on the growth and hematological performance of juvenile red tilapia. This study used an experimental method with a completely randomized design consisting of five treatments with four replications, namely the fish were given food for 28 days (A), 7 days of feed restriction followed by 21 days of refeeding (B), 14 days of feed restriction followed by 14 days of refeeding (C), 21 days of feed restriction followed by 7 days of refeeding (D) and 28 days of feed restriction (E). The parameters observed were growth, biometry, and hematological values. The results showed that during the feed restriction period there was a significant decrease in growth, condition factors, hepatosomatic index along with viscerosomatic index with the length of time for feed restriction, although fasting for up to 28 days did not cause fish mortality. Hematological parameters such as RBCs, WBCs, hematocrit, and hemoglobin decreased significantly, but after re-feeding, they increased significantly in consecutive times. As for the blood glucose levels decreased during feed restriction and gradually increased after re-feeding. This study stated that feed restriction ﬁsh gained weight continuously during re-feeding, but no displaying compensatory growth. The results suggested that the decrease in hematological parameters did not indicate stress levels in fish, but reflected a lack of nutrition condition.

Download Full-text

The Effects of Lead Toxicity on the Hematological Parameters Common Carp (Cyprinus carpio) at Varying Salinity Levels

Iranian Jornal of Toxicology ◽

10.32598/ijt.14.1.1 ◽

2020 ◽

Vol 14 (1) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Zahra Tulaby Dezfuly ◽

◽

Amir Aramoon ◽

Mojtaba Alishahi ◽

Mostafa Halimi ◽

...

Keyword(s):

Blood Cells ◽

Negative Impact ◽

Hematological Parameters ◽

Juvenile Fish ◽

White Blood Cells ◽

Lead Toxicity ◽

Inverse Correlation ◽

Aquatic Organisms ◽

Control Group ◽

Almost All

Background: Lead (Pb) is an important metal used in industries, mining and agriculture. It is equally important to the aquatic ecosystems and has negative impact on the health of aquatic organisms. Methods: The LC50 of lead toxicity at varying salinities was first determined for 96 hours. Then juvenile fish were transferred to tanks individually and were exposed to 0, 15, 30, 60 and 120 mg/l of Pb at varying salt concentration of 0, 0.1, 0.2 or 0.4 g/l. The blood samples were taken after the 4th day and the hematological parameters were evaluated. Results: Red Blood Cells (RBC) and White Blood Cells (WBC) were decreased in almost all lead treated groups. The Hb levels decreased consistent with increasing lead concentrations (P<0.05). The mean corpuscular volume, hemoglobin and the concentration were not affected by the lead concentrations compared to those for the control group. The WBC and RBC counts showed an inverse correlation with the lead concentrations in the Pb-exposed fish. A direct correlation was also observed in the lead concentration and the heterophile rate (P<0.05). Conclusion: The results showed that salinity did not affect the hematological parameters, but the LC50 values decreased with increasing salinity.

Download Full-text