scholarly journals THE EFFECT OF PIR-20 COMPOUND ON COGNITIVE DEFICIT REDUCTION IN EXPERIMENTAL GLOBAL CEREBRAL ISCHEMIA IN RATS

2021 ◽  
Vol 11 (3) ◽  
pp. 26-28
Author(s):  
Natalia Shabanova ◽  
Anastasia Gerashchenko ◽  
Andrey Voronkov
Keyword(s):  
Cerebral Ischemia ◽  
Tween 80 ◽  
Pyrimidine Derivative ◽  
Negative Control ◽  
Global Cerebral Ischemia ◽  
Control Group ◽  
Reference Drug ◽  
Deficit Reduction ◽  
Male Wistar Rats ◽  
Extrapolation Escape Test

A study was conducted to assess the effect of a new pyrimidine derivative PIR-20 (50 mg/kg) on the development of cognitive deficits in the conditions of global cerebral ischemia in rats. The study was performed on 40 male Wistar rats weighing 200–220 g, divided into 4 groups of 10 individuals. False-operated rats and negative control animals were injected with a suspension of purified water and tween-80, the third group of animals received Cavinton (3,2 mg/kg), the fourth — PIR-20 (50 mg/kg). All test subjects were injected intraperitoneally for ten days prior to surgery. The number of dives increased to 100%, while the decision-making time decreased by 55,2% (p<0,05) in the extrapolation escape test against the background of the PIR-20 compound administration. 75% of the animals treated with PIR-20 did not re-visit the dark compartment, and the time of entering the dark chamber increased by 172,9% (p<0,05) as compared to the group of negative control rats in the test of passive avoidance of the avesir environment. It was confirmed that the studied compound PIR-20 contributes to the improvement of cognitive and mnestic functions, which is confirmed by the results of tests of passive and active aversive environment avoidance. The obtained effect exceeded the results of the control group and the reference drug Cavinton.

scholarly journals al deficits in experimental global cerebral ischemia in rats

2020 ◽  
Vol 10 (4) ◽  
pp. 26-28
Author(s):  
Natalia Shabanova ◽  
Anastasia Gerashchenko ◽  
Andrey Voronkov
Keyword(s):  
Cerebral Ischemia ◽  
Brain Ischemia ◽  
Neurological Status ◽  
Pyrimidine Derivative ◽  
Neurological Deficits ◽  
Global Cerebral Ischemia ◽  
Control Group ◽  
Global Brain Ischemia ◽  
Reference Drug ◽  
Global Brain

This study was aimed to assess the effect of a new pyrimidine derivative (PIR-12 50 mg/kg) on survival and neurological deficits in rat global brain ischemia. It has been confirmed that the investigated compound PIR-12 contributes to an increase in survival up to 80% and a decrease in neurological status by 73,3% compared to the control group of animals and exceeds the strength of the effect of the reference drug Cavinton by 30% and 22,48%, respectively.

scholarly journals STUDY OF CEREBROTROPIC DOSE-DEPENDENT EFFECT OF PYRIMIDINE DERIVATIVE UNDER PIR-9 CODE AGAINST THE BACKGROUND OF EXPERIMENTAL CEREBRAL ISCHEMIA IN RATS

2019 ◽  
Vol 6 (6) ◽  
pp. 548-567
Author(s):  
A. V. Voronkov ◽  
N. B. Shabanova ◽  
M. P. Voronkova ◽  
T. A. Lysenko
Keyword(s):  
Cerebral Ischemia ◽  
Carotid Arteries ◽  
Tween 80 ◽  
Pyrimidine Derivative ◽  
Behavioral Activity ◽  
Test Substance ◽  
Reference Drug ◽  
Dose Dependent ◽  
Common Carotid Arteries ◽  
Experimental Cerebral Ischemia

Nowadays, the incidence of cerebrovascular disease is steadily increasing. Disorders of cerebral circulation contribute to the increase in the degree of mortality, disability, and incapacitation of the population. An extensive arsenal of drugs with cerebroprotective effects does not satisfy clinical specialists. In this connection, there is an obvious need for new compounds exhibiting cerebroprotropic properties, as well as those able of improving the prognosis of the course of ischemic genesis pathologies.The aimof the article is to study the dose-dependent cerebrotropic effect of a pyrimidine derivative under PIR-9 code against the background of experimental cerebral ischemia in rats.Materials and methods. The experiment was conducted on 140 male Wistar rats (m=170–190 g) divided into 7 equal groups. Pyrimidine derivative PIR – 9 (25, 50 and 100 mg/kg), Vinpocetine (3.2 mg/kg) and Cinnarizine (5.6 mg/kg) suspension of purified water and Tween-80 were used as the studied substances. Experimental cerebral ischemia was reproduced by irreversible occlusion of common carotid arteries (chloral hydrate anesthesia – 350 mg/kg). Experimental substances, reference preparations and purified water were administered prophylactically within 10 days before surgery. One day later the survival, neurological deficiency, behavioral activity, changes in cognitive-mnestic functions, as well as some indicators of brain energy exchange were evaluated.Results.In an experimental study of the cerebrotropic effect of the substance under PIR-9 code (pyrimidine-4-(1H)-one derivative) in various dosages against the background of irreversible occlusion of the common carotid arteries, a decrease in neurological, behavioral, mnestic and cognitive defects has been established. Hereby, the best effect was observed against the background of the administration of the compound PIR-9 at the dose of 50 mg kg. In addition, the prophylactic administration of the test substance PIR-9 (50 mg/kg) has shown the improvement of the energy metabolism in the postischemic period.Conclusion. In the course of the study it was established that the substance under the laboratory code PIR-9 exhibited the most pronounced cerebroprotective effect at the dose of 50 mg/ kg, which was not inferior in its strength to the reference drug Cinnarizine and exceeding Vinpocetine.

scholarly journals The effect of PIR-9 compound on markers of apoptosis in experimental focal cerebral ischemia in rats

2019 ◽  
Vol 9 (1) ◽  
pp. 83-85
Author(s):  
A.V. Voronkov ◽  
N.B. Shabanova ◽  
M.P. Voronkova ◽  
T.A. Lysenko ◽  
A.V. Arlt ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Rat Brain ◽  
Focal Cerebral Ischemia ◽  
Pyrimidine Derivative ◽  
Negative Control ◽  
Reference Drug ◽  
Apoptosis Markers

A study to assess the effect of a new pyrimidine derivative (PIR-9 at a dose of 50 mg/kg) on apoptosis markers in experimental focal cerebral ischemia of the rat brain. It has been confirmed that the investigated compound PIR-9 contributes to a decrease in the concentration of TNFα by 34,36% (p<0,05) as compared to that in rats treated with a reference drug Cavinton (3,2 mg/kg) and has an effect comparable in effect to Gliatilin (60 mg/kg). The concentration of AIF in rats that received compound PIR-9 was 29,99% (p<0,05) less than the group of negative control rats.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

Natural hypothermia immediately after transient global cerebral ischemia induced by spontaneous subarachnoid hemorrhage

2003 ◽  
Vol 98 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kiyoshi Takagi ◽  
Yoshiaki Tsuchiya ◽  
Kimiko Okinaga ◽  
Masafumi Hirata ◽  
Tadayoshi Nakagomi ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Body Temperature ◽  
Global Cerebral Ischemia ◽  
Control Group ◽  
Mean Body Temperature ◽  
Content Type ◽  
Transient Global Cerebral Ischemia ◽  
Gcs Score ◽  
Spontaneous Subarachnoid Hemorrhage ◽  
Fine Print

Object. Spontaneous subarachnoid hemorrhage (SAH) has an aspect of graded transient global cerebral ischemia. The purpose of the present study was the documentation of sequential changes in body temperature immediately after SAH-induced transient global cerebral ischemia in humans. Methods. Patients admitted within 12 hours after the initial onset of SAH were examined retrospectively (426 patients). Patients with unruptured cerebral aneurysms served as a control group (73 patients). Body temperature measured at the axilla on admission was analyzed. The grade of SAH was established according to the Glasgow Coma Scale (GCS): Grade I, GCS Score 15; Grade II, GCS Score 11 to 14; Grade III, GCS Score 8 to 10; Grade IV, GCS Score 4 to 7; and Grade V, GCS Score 3. The mean body temperature of patients in the control group was 36.49 ± 0.45°C (mean ± standard deviation). The mean body temperature of patients in the SAH group who had been admitted within 4 hours of onset for Grades I to V were significantly different (p < 0.001, analysis of variance [ANOVA]): 36.26 ± 0.7°C, 59 patients; 35.98 ± 0.85°C, 73 patients; 35.52 ± 0.79°C, 25 patients; 35.9 ± 1.09°C, 108 patients; and 35.56 ± 1.14°C, 73 patients, respectively. These values were significantly lower than those in control volunteers, except for patients with Grade I SAH. The reduction in body temperature was unrelated to the location of the cerebral aneurysm and was not the product of circadian rhythm. The temperatures of patients in the SAH group who were admitted beyond 4 hours after onset for each grade were significantly different (p < 0.01, ANOVA): 36.8 ± 0.91°C, 36 patients; 36.74 ± 0.68°C, 31 patients; 36.73 ± 0.38°C, three patients; 37.41 ± 1.37°C, 17 patients; and 38.9°C, one patient, respectively. These values were significantly higher than those in patients admitted within 4 hours of SAH onset for all grades except Grade V, and significantly higher than control values in patients with Grades I and IV SAH. Conclusions. These results indicate that body temperature falls and then rises immediately after the SAH-induced transient global cerebral ischemia without cardiac arrest in humans. The reduction in temperature may be a natural cerebral protection mechanism that is activated shortly after ischemic insult.

scholarly journals Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

2016 ◽  
Vol 18 (1) ◽  
pp. 113-124 ◽  
Author(s):  
G.H. CUNHA ◽  
F.V. FECHINE ◽  
F.A. FROTA BEZERRA ◽  
M.O. MORAES ◽  
E.R. SILVEIRA ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vascular Reactivity ◽  
Antihypertensive Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Hypertensive Rats ◽  
Male Wistar Rats ◽  
Main Components

ABSTRACT The aim of this study was to characterize components of the EOAz and its hexane (HFEOAz), chloroform (CFEOAz) and methanol (MFEOAz) fractions, and its antihypertensive effect. EOAz was extracted from leaves by hydrodistillation. Aliquot was subjected to selective desorption with silica gel column and eluted with hexane, chloroform and methanol. The components of the EOAz and fractions were analyzed by gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy of hydrogen. Experiments of vascular reactivity were performed with isolated aortic rings of male Wistar rats. Antihypertensive effect was evaluated in hypertensive rats submitted to the inhibition of synthesis of nitric oxide. Blood pressure was measured indirectly by tail plethysmography. MFEOAz showed the lowest EC50 (150.45 µg/mL), 1,8-cineole (27.81%) and terpinen-4-ol (57.35%) as main components. Single administration by nasogastric tube of EOAz, fractions and captopril significantly reduced the blood pressure of hypertensive rats, when compared to animals of the negative control group with distilled water. In conclusion, the potency of the MFEOAz was higher than that of EOAz and other fractions. The antihypertensive effect of EOAz and fractions was similar, higher than the negative control and lower than that of captopril.

Cardiovascular responses to global cerebral ischemia: role of excitatory amino acids in the ventrolateral medullary pressor area

1993 ◽  
Vol 78 (6) ◽  
pp. 922-928 ◽  
Author(s):  
Robert F. Heary ◽  
Allen H. Maniker ◽  
Abbott J. Krieger ◽  
Hreday N. Sapru
Keyword(s):  
Amino Acids ◽  
Cerebral Ischemia ◽  
Excitatory Amino Acids ◽  
Excitatory Amino Acid ◽  
Cardiovascular Responses ◽  
Global Cerebral Ischemia ◽  
Content Type ◽  
Acid Receptor ◽  
Male Wistar Rats

✓ The object of this study was to investigate the role of the ventrolateral medullary pressor area in mediating the cardiovascular responses to experimentally induced global cerebral ischemia, and to test if excitatory amino acids or acetylcholine are the transmitters released in this brain region during these responses. The cerebral ischemic response was elicited in pentobarbital-anesthetized, artificially ventilated male Wistar rats by bilateral ligation of vertebral arteries followed by temporary clamping of the common carotid arteries. The pressor area was identified by microinjections of L-glutamate. Inhibition of neurons in this area by microinjections of muscimol, a γ-aminobutyric acid receptor agonist, abolished the ischemic response, which demonstrated that this area is important in mediating these responses. Microinjections of a broad-spectrum excitatory amino acid receptor blocker (kynurenate), of specific antagonists for N-methyl-D-aspartic acid (NMDA) and non-NMDA receptors (injected alone or in combination), and of atropine failed to block the ischemic responses. These results indicate that: 1) the ventrolateral medullary pressor area mediates pressor responses to cerebral ischemia, and 2) excitatory amino acids or acetylcholine in this area do not mediate the cardiovascular responses to cerebral ischemia.

Prevention and therapy of 1,2-dimethyl hydrazine induced colon carcinogenesis by Ferula assafoetida hydroalcoholic extract / 1,2-dimetilhidrazin ile indüklenmiş kalın bağırsak kanserlerinin Ferula assa-foetida ekstraktı kullanılarak önlenmesi ve tedavisi

2015 ◽  
Vol 40 (5) ◽  
Author(s):  
Fatemeh Torabi ◽  
Abolfazl Dadkhah ◽  
Faezeh Fatemi ◽  
Salome Dini ◽  
Mohsen Taghizadeh ◽  
...  
Keyword(s):  
Colon Carcinogenesis ◽  
Negative Control Group ◽  
Negative Control ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Hydroalcoholic Extract ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters ◽  
Ferula Assafoetida

AbstractObjective: In this study, we have evaluated the chemopreventive and chemotherapeutic effects of Ferula assa-foetida hydroalcoholic extract in 1, 2-dimethyl hydrazine (DMH) induced colon carcinogenesis.Methods: Male Wistar rats were divided into 6 groups: a negative control group without DMH; control group with injected DMH (20 mg/Kg b.w) and four groups receiving DMH +F. assa- foetida extract (6.25 and 12.5 mg/Kg b.w) as chemopreventive and chemotherapeutic groups. The effects of the extracts were assessed by estimating the hepatic oxidative stress/antioxidant parameters such as malondialdehyde, glutathione and ferric reducing ability of plasma (MDA, GSH, FRAP) and the detoxification enzymes; glutathione S-transferase and Cytochrome PResults: The results showed that the F. assa-foetida extracts markedly reversed the increased levels of CYPConclusion: The achieved results suggested the beneficial effect of the extracts on DMH metabolic processes in the colon indicating its chemopreventive and chemotherapeutic effects on colon carcinogenesis induced by DMH.

scholarly journals Effects of Steroidal and Nonsteroidal Drugs on Tooth Movement and Root Resorption in the Rat Molar

2009 ◽  
Vol 79 (4) ◽  
pp. 715-726 ◽  
Author(s):  
Carmen Gonzales ◽  
Hitoshi Hotokezaka ◽  
Ken-Ichiro Matsuo ◽  
Tatsunori Shibazaki ◽  
Joseph H. Yozgatian ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Three Dimensional ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Experimental Control ◽  
Male Wistar Rats

Abstract Objective: To test the hypothesis that the administration of aspirin, acetaminophen, meloxicam, celecoxib, and prednisolone have no effect on root resorption and tooth movement. Materials and Methods: A mesial force of 50 g was applied to the left maxillary first molars of sixty 10-week-old male Wistar rats using nickel titanium closed coil springs attached to the cervical area of the incisors. The rats were randomly divided into 12 groups of 5 each. High and low doses of aspirin, acetaminophen, meloxicam, celecoxib, and prednisolone were administered via drinking water for 2 weeks. The experimental control group had tooth movement but received no drug. The negative control group received neither tooth movement nor drugs. The amount of tooth movement was measured on digitized lateral cephalometric radiographs. Rats were sacrificed after 2 weeks. Mesial and distal roots (distobuccal and distopalatal) were examined using scanning electron and three-dimensional (3D) scanning laser microscopes. The surface area, depth, volume, and roughness of the root resorption craters were measured. Results: When compared with experimental control rats, only prednisolone- and high-dose celecoxib-treated groups showed significantly less root resorption and less tooth movement. Although low dose celecoxib-treated group significantly decreased the tooth movement, root resorption was similar to the control group. Furthermore, resorption craters showed a smoother surface in the prednisolone-treated rats. Conclusions: The hypothesis was rejected. Administration of prednisolone and high-dose celecoxib reduces root resorption and interferes with tooth movement in rats. Both drugs may interfere in the arachidonic acid cascade depending on dose thresholds.

scholarly journals The Use of Aloe vera Gel on Scar Collagen

2021 ◽  
Vol 4 (2) ◽  
pp. 89
Author(s):  
Amania Nur Alam ◽  
Muhammad Jailani ◽  
Siti Hajar
Keyword(s):  
Aloe Vera ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Collagen Formation ◽  
Aloe Vera Gel ◽  
Post Test ◽  
Male Wistar Rats

Aloe vera have active chemicals substances including saponins, tannins, flavonides, and polyphenols. Saponin takes a role as a disinfectant, so it is normally effective for healing any open wounds, while tannins have ability to be an antiseptic. This study aims to see how the effect of the Aloe vera gel on wound collagen, and too inspect which is the most effective concentration of the Aloe vera gel in the formation of wound collagen in wistar strains (Rattus novergicus) rats. This study uses a post-test only method with control group design. The subjects of this study were 28 male Wistar rats (Rattus novergicus). The subjects were divided into 4 groups consisting of 1 negative control group and 3 treated groups, the control group was not given any treatment, while the treated group was given the Aloe vera gel with each concentrations of 12.5%, 25%, and 50% respectively. The experiment was started by making a wound on the back of the rat which then be followed by applying the gel to the wound for 14 days. The results showed the group with the 50% Aloe vera gel treatment had the best collagen formation, followed by a concentration of 25%, 12.5%, and the control group. Therefore, the used of Aloe vera has given good change in scar collagen, and the best scar collagen can be viewed in the group with 50% concentrate of Aloe vera.

Sign in / Sign up

Export Citation Format

Share Document