scholarly journals GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR (Rattus norvegicus) YANG DIBERIKAN VITAMIN C (Asam askorbat) DOSIS TINGGI

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Juwita P. S. Baharuddin
Keyword(s):  
Vitamin C ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Histopathological Features ◽  
Acute Gastritis ◽  
Group A ◽  
Mucosal Lining ◽  
High Doses ◽  
Group B ◽  
Normal Stomach

Abstract: Background: Vitamin C is one of the chemicals which are acidic and therefore is able to irritate the stomach lining if consumed in excessive amount. Objective: To reveal the histopathological features of the stomach of wistar rats supplemented with high doses of vitamin C. Method: Experimental study employing eight wistar rats divided into three groups. Rats in group A were fed with regular pellets for 12 days. Rats in group B were fed with regular pellets and were supplemented with vitamin C at 6 mg/day for 12 days. Rats in group C were fed with regular pellets and were supplemented with vitamin C at 8 mg/day for 12 day. Results: Rats in group A showed normal stomach features. Rats in group B revealed PMN inflammatory cells and hiperemy on the mucosal lining, submucosa, and muscularis mucosae. Moreover, edema on the mucosal lining was also seen in the rats in this group. Similar histopathological features were also demonstrated by the rats in group C. In this group the number of PMNs and hiperemy was greater than that of group B; in addition, cell regeneration was also seen in this group. Conclusion: Supplementation of high doses of vitamin C at 6 mg/kg and 8 mg/kg of body weight per day for 12 days may lead to changes in histopathological features of the stomach of wistar rats. Keywords: acute gastritis, vitamin C.     Abstrak: Latar Belakang: vitamin C merupakan salah satu bahan kimia yang bersifat asam, sehingga apabila dikonsumsi berlebihan dapat mengiritasi lambung. Tujuan: Untuk mengetahui gambaran histopatologi lambung tikus wistar yang diberikan  vitamin C dosis tinggi. Metode: Penelitian eksperimental dengan 8 ekor tikus wistar dibagi menjadi 3 kelompok. Kelompok A diberikan pelet biasa selama 12 hari. Kelompok B diberikan pelet biasa dan vitamin C 6 mg/hari selama 12 hari. Kelompok C diberikan pelet biasa dan vitamin C 8 mg/hari selama 12 hari. Hasil: Pada kelompok A didapatkan gambaran lambung yang normal.  Kelompok B terlihat sel-sel radang PMN dan hiperemi pada lapisan  mukosa, sub mukosa dan muskularis, pada kelompok ini juga terlihat edema pada lapisan mukosa. Kelompok C terlihat pada lapisan mukosa, submukosa dan muskularis adanya sel-sel radang PMN dan hiperemi yang lebih banyak dibandingkan kelompok B, selain itu juga terlihat regenerasi sel. Kesimpulan: Pemberian vitamin C dosis tinggi yaitu 6 mg/hari dan 8 mg/hari selama 12 hari dapat mengakibatkan perubahan gambaran histopatologi lambung. Kata Kunci: Gastritis akut, vitamin C.

scholarly journals GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR (Rattus norvegicus) YANG DIBERIKAN LENGKUAS (Alpinia galanga Willd) SETELAH DIINDUKSI OLEH ASAM MEFENAMAT

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Juita Pasaribu ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Control Group ◽  
Treatment Groups ◽  
Acute Gastritis ◽  
Group A ◽  
Mucosal Lining ◽  
Group B ◽  
Group D

Abstrak: Gastritis akut merupakan proses inflamasi yang bersifat akut dan biasanya terjadi pada bagian permukaan mukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan asam mefenamat dalam dosis yang berlebihan. Pada penelitian yang telah dilakukan sebelumnya didapatkan bahwa lengkuas dapat mengurangi terjadinya radang akut. Tujuan penelitian ini adalah untuk mengetahui gambaran histopatologi lambung tikus wistar yang diberikan lengkuas setelah diinduksi dengan asam mefenamat. Penelitian ini menggunakan metode eksperimental dengan sampel 11 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan asam mefenamat 10 mg selama 7 hari. Kelompok C diberikan asam mefenamat 10 mg selama 7 hari kemudian diberikan perasan lengkuas selama 7 hari. Kelompok D diberikan asam mefenamat 10 mg kemudian tidak diberikan perlakuan selama 7 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: lengkuas dapat mengurangi sel-sel radang PMN pada mukosa lambung yang diberikan dengan asam mefenamat. Kata kunci: Lengkuas, Asam Mefenamat, Gastritis akut.   Abstract : acute gastritis is an acute inflammatory process normally occurring in the mucosal lining of the stomach. This disease is caused by a variety of factors, one of which is the use of mefenamat acid in large doses. Previous research has revealed that galangal can be used to reduce acute inflammation. The objective of this research was to reveal the histopathological pictures of stomach of wistar rats that treatment with galangal after induce by mefenamat acid. This study is an experimental, employing 11 wistar rats which were assigned for four treatment groups. Group A was the control group in which the rats received no treatment. Rats in group B were administered mefenamat acid 10 mg for 7 days. In group C, the rats were also administered mefenamat acid 10 mg for 7 days and then received galangal distillation for 7 days. In group D, rats were administered mefenamat acid 10 mg for 7 days and then for 7 days without receiving anymore mefenamat acid or galangal distillation. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: galangal can reduce PMN inflammatory cells in mucosal lining of the stomach exposed to mefenamat acid. Key words: galangal, mefenamat acid,  acute gastritis.

scholarly journals Gambaran histopatologik lambung tikus Wistar (Rattus norvegicus) yang diberikan air perasan jeruk nipis (Citrus aurantifolia)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Suhaidir Laomo ◽  
Lily L. Loho ◽  
Carla F. Kairupan
Keyword(s):  
Vitamin C ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Herbal Remedy ◽  
Gastric Gland ◽  
High Dose ◽  
Gastric Glands ◽  
Histopathological Features ◽  
Group A ◽  
Group B

Abstract: Lime squash is often used as a herbal remedy for gastritis, even though it is acidic. It contains citric acid and vitamin C which in certain doses can be irritant to the stomach. Nevertheless, lime squash also contains antioxidants such as limonene, flavonoids, and vitamin C, that play certain roles in improving the protection of the stomach and the ability of cell regeneration, and as anti-inflammatory agents. This study aimed to reveal the histopathological features of the gaster of Wistar rats (Rattus norvegicus) treated with lime squash. This was an experimental laboratory study using 15 adult Wistar rats divided into three groups (five rats in each group). Group A (negative control) was given no treatment; group B and C were treated with lime squash 0.5 and 1.56 ml/day, respectively. Lime squash was given in a single dose for seven consecutive days. Rats were terminated on day eight. The results revealed that the gaster of Wistar rats in group A were similar to that of the normal gaster, while group B and C showed no signs of inflammation and the gastric gland cells appeared enlarged and dense. Conclusion: Histopathological features of the gaster of Wistar rats, treated with either low or high dose (three-fold) lime squash, showed no signs of abnormalities such as inflammation, however, there were enlarged glandular cells which might be related to increased gastric mucosal defence.Keywords: lime, gastric histopathological features, inflammatory cells, gastric glands Abstrak: Air perasan jeruk nipis sering digunakan sebagai obat herbal terhadap gastritis, meskipun bersifat asam. Air perasan jeruk nipis mengandung asam sitrat yang pada dosis tertentu menjadi bahan iritan terhadap lambung. Namun demikian, kandungan antioksidan seperti limonene, flavonoid dan vitamin C berperan dalam meningkatkan proteksi lambung dan kemampuan regenerasi sel, serta sebagai anti inflamasi. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik lambung tikus wistar yang diberikan air perasan jeruk nipis. Jenis penelitian ialah eksperimental menggunakan 15 ekor tikus Wistar yang dibagi dalam tiga kelompok (lima ekor tikus tiap kelompok). Kelompok A (kontrol negatif) tidak diberi perlakuan; kelompok B dan C masing-masing diberi air perasan jeruk nipis 0,5 ml/hari dan 1,56 ml/hari dosis tunggal selama tujuh hari, Semua tikus diterminasi pada hari ke-8. Hasil yang didapatkan pada gambaran histopatologik lambung tikus wistar yaitu: kelompok A sesuai dengan gambaran lambung normal; kelompok B dan C tidak menunjukkan tanda-tanda peradangan namun sel-sel kelenjar lambung tampak membesar dan padat. Simpulan: Gambaran histopatologik lambung tikus wistar yang diberi air perasan jeruk nipis, baik dosis rendah maupun tinggi (tiga kali lipat) tidak menunjukkan tanda-tanda kelainan seperti peradangan, melainkan tampak terjadi pembesaran sel-sel kelenjar lambung yang mungkin berhubungan dengan peningkatan pertahanan mukosa lambung. Kata kunci: jeruk nipis, gambaran histopatologik lambung, sel radang, kelenjar lambung

scholarly journals Pengaruh cabe rawit terhadap gambaran histopatologik lambung tikus Wistar yang diinduksi aspirin

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abel T. Bawulele ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Changes ◽  
Acute Gastritis ◽  
Group B ◽  
Group D ◽  
Mucosa Epithelium

Abstract: Cayenne pepper (Capsicum frustescens L ) is commonly in daily food. This study aimed to determine the effect of cayenne pepper to histopathological changes in gaster of Wistar rats induced by aspirin. This was an experimental study. Subjects were Wistar rats consisted of 5 rats as the negative control and 20 rats as the treatment group. Group A, the negative control group, was untreated; group B was treated with aspirin 21mg/day for 10 days; group C was treated with aspirin 21mg/day for 10 days together with cayenne pepper 84mg once daily; group D was treated with aspirin 21mg/day for 10 days followed by cayenne 84mg twice daily; and group E was treated with aspirin 21mg/day for 10 days and then was untreated for 3 days. The histopathological changes in group C showed a lot of inflammatory cells and erosion of gastric mucosa epithelium compared to group B, D, and E. Group D also showed inflammatory cells but no erosion as in group C. Group E had less inflammatory cells than group B, C, and D. Conclusion: Administration of aspirin 21mg/kg BW for 10 days led to acute gastritis in Wistar rat. Administration of aspirin together with cayenne 84mg/day led to acute gastritis and erosion of gastric mucosa epithelium. However, administration of cayenne pepper 168mg/day after aspirin led to acute gastritis without erosionKeywords: pepper, gaster, aspirinAbstrak : Cabe Rawit (Capsicum frustescens L.) banyak dijumpai dalam makanan sehari-hari. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian cabe rawit terhadap gambaran histopatologik lambung tikus wistar yang diinduksi aspirin. Jenis penelitian ialah eksprimental. Subjek penelitian ialah tikus wistar, yang dibagi atas: 5 ekor untuk kontrol negatif dan 20 ekor untuk perlakuan. Kelompok A (kontrol negatif) tidak diberi perlakuan; kelompok B diberikan aspirin 21mg/hari selama 10 hari; kelompok C diberikan aspirin 21mg/hari selama 10 hari bersama cabe rawit 84mg sekali sehari; kelompok D diberikan aspirin 21mg/hari selama 10 hari kemudian dilanjutkan pemberian cabe rawit 84mg selama 3 hari 2 kali sehari; dan kelompok E diberikan aspirin 21mg/hari selama 10 hari kemudian tidak diberi perlakuan selama 3 hari. Hasil penelitian menunjukkan bahwa pada kelompok C terlihat banyak sel-sel radang disertai erosi epitel mukosa lambung dibanding dengan kelompok B, D, dan E. Pada kelompok D pemberian aspirin dilanjutkan dengan cabe rawit dosis 2 kali sehari masih terdapat sel-sel radang tetapi tidak terjadi erosi seperti pada kelompok C. Kelompok E memiliki sel-sel radang yang lebih sedikit dibandingkan kelompok B, C, dan D. Simpulan: Pemberian aspirin 21mg/hari selama 10 hari menyebabkan gastritis akut pada tikus wistar. Pemberian aspirin bersama cabe rawit 84mg/hari menyebabkan gastritis akut erosi sedangkan pemberian cabe rawit 168mg/hari setelah diberi aspirin menyebabkan gastritis akut tanpa erosi.Kata kunci: cabe rawit, lambung, aspirin

scholarly journals EFEK PEMBERIAN ANABOLIK ANDROGENIK STEROID INJEKSI DOSIS RENDAH DAN TINGGI TERHADAP GAMBARAN HISTOPATOLOGI HATI DAN OTOT RANGKA TIKUS WISTAR (Rattus Novergicus)

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Pamela K. Sari ◽  
Poppy M. Lintong ◽  
Lily L. Loho
Keyword(s):  
Protein Synthesis ◽  
Wistar Rats ◽  
Low Dose ◽  
Anabolic Steroids ◽  
Inflammatory Cells ◽  
High Dose ◽  
Group A ◽  
Androgenic Anabolic Steroids ◽  
Group B ◽  
Group D

Abstract: Androgenic-anabolic steroids (AAS) are synthetic derivatives of the male hormone endogenous testosterone that stimulates anabolic (protein synthesis) and androgenic effects (masculinization). Long-term usage of AAS can result in liver damage. However, physiological concentrations of testosterone can stimulate protein synthesis which lead to an increase in muscle size, body mass, and endurance. This study aimed to determine the histopathology of liver and skeletal muscles of wistar rats that were given low dose and high dose injection of AAS. Subjects were 21 wistar rats divided into 7 groups. Group A was given standard pellets for 56 days (negative control), terminated on days 29,43, and 57. Group B was treated with low-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group C was treated with low-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group D was treated with low-dose AAS injection and standard pellets for 56 days, terminated on day 57. Group E was treated with high-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group F was treated with high-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group G was treated with high-dose AAS injection and standard pellets for 56 days, terminated on day 57. The results showed that the histopathology of liver and muscles in group A was still normal. In group B, the architecture of liver was still normal with a few inflammatory cells around the Kiernan triangle while in muscle the ratio of myofibril diameter was 1.28:1. In group C and group D, there were widening of the hepatic artery, bile duct, and portal vein containing blood fibrin, and inflammatory cells around the Kiernan triangle. The ratio of myofibril diameter was 1.43:1 in group C and 2.14:1 in group D. In group E, F and G, there were micro-vesicular fatty cells in the peripheral part of the liver meanwhile the myofibril diameter ratio of the muscles in group E was 1.43:1, group F 2.1:1, and group G 2.28:1. Conclusion: Administration of AAS injection of low dose and high dose for less than 4 weeks could result in inflammation, dilation of the portal vein, hepatic artery and bile duct meanwhile administration of AAS for over 4 weeks could ressult in focal fatty liver (steatosis). The administration of AAS injection of low dose and high dose for 4,6 and 8 weeks reslutid in enlargement of skeletal muscle (muscle hypertrophy).Keywords: androgenic-anabolic steroids, liver, skeletal muscleAbstrak: Anabolik Androgenik Steroid (AAS) adalah derivat sintetis dari hormon sex testosteron endogen pria, yang merangsang efek anabolik (sintesis protein) dan androgenik (maskulinisasi). Penggunaan AAS jangka panjang dapat mengakibatkan terjadinya kerusakan hati namun secara fisiologi testosteron dapat menstimulasi sintesis protein sehinggaberdampak pada peningkatan ukuran otot, massa tubuh dan ketahanan tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histopatologi hati dan otot rangka wistar yang diberikan AAS injeksi dosis rendah dan dosis tinggi. Subjek penelitian 21 ekor wistar yang dibagi menjadi 7 kelompok. Kelompok A diberi pelet standar selama 56 hari (kontrol negatif), terminasi pada hari ke-29, 43, dan 57. Kelompok B diberi perlakuan AAS injeksi dosis rendah dan pelet standar selama 28 hari, terminasi hari ke-29. Kelompok C diberi AAS injeksi dosis rendah dan pelet standar selama 42 hari, terminasi hari ke-43. Kelompok D diberi AAS injeksi dosis rendah dan pelet standar selama 56 hari, terminasi hari ke-57. Kelompok E diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 28 hari, terminasi hari ke-29. Kelompok F diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 42 hari, terminasi hari ke-43. Kelompok G diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 56 hari, terminasi hari ke-57. Hasil penelitian menunjukkan pada kelompok A didapatkan gambaran histopatologi hati normal sedangkan pada otot tidak terdapat perubahan. Pada kelompok B didapatkan arsitektur hati masih normal dengan sedikit sel radang disekitar segitiga Kiernan sedangkan pada otot terlihat diameter miofibril ratio 1,28:1. Pada kelompok C dan D terlihat pelebaran arteri hepatika, duktus biliaris, dan vena porta yang berisi fibrin darah, serta sel-sel radang di sekitar segitiga Kiernan. Pada kelompok C diameter miofibril ratio 1,43;1 dan pada kelompok D 2,14:1. Pada kelompok E, F dan G terdapat sel-sel perlemakan mikrovesikuler di perifer sedangkan pada otot diameter miofibril ratio kelompok E 1,43:1, kelompok F 2,1:1, dan kelompok G 2,28:1. Simpulan: Pada pemberian AAS injeksi dosis rendah dan dosis tinggi kurang dari 4 minggu terjadi peradangan hati, pelebaran vena porta, arteri hepatika dan duktus biliaris sedangkan lebih dari 4 minggu terdapat perlemakan (steatosis) fokal hati. Pemberian AAS injeksi dosis rendah dan tinggi dalam waktu 4,6 dan 8 minggu menunjukkan pembesaran otot rangka (hipertrofi otot).Kata kunci: AAS, hati, otot rangka

scholarly journals Effects of Moringa oleifera on the developing cerebrum of young wistar rats

1970 ◽  
Vol 8 (1) ◽  
Author(s):  
A Z Abijo ◽  
O O Adeeyo ◽  
O A Komolafe ◽  
O S Saka ◽  
V K Abodunrin
Keyword(s):  
Frontal Cortex ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
The Body ◽  
Brain Weight ◽  
Control Group ◽  
Significant Difference ◽  
Group A ◽  
High Doses ◽  
Group B

The study evaluated the effects of moringa oleifeira on the histoarchitecture of the cerebral cortex, the body weight and brain weight of young wistar rats. Fifteen (15) young wistar rats of both sexes weighing 20-30g were used for this study. They were randomly assigned into three groups (A, B and C) of five rats each. Group A served as control and received distilled water, group B and C received 100 mg/kg and 200mg/kg of moringa oleifera respectively. Treatment lasted for a period of 6 weeks (orally). Rats were weighed and sacrificed under ketamine (30 mg/kg) anaesthesia. The cerebrum was harvested and fixed immediately in 10% formolcalcium, for further histological processing. One-way ANOVA was used to analyze data, followed by Newan-Keuls (SNK) for multiple comparisons. The results showed that there was significant increase in the feed intake of animals in groups B and C starting from the 4th week of administration. There was no significant difference in the relative brain weight and the mean weight of the rats in group B and C when compared with group A. Histological findings revealed that there was slight distortion in group B and more distortion in group C when compared with the normal histoarchitecture in control group A. The results obtained from this study showed that high doses of Moringa oleifera caused damage of some parts of histoarchitecture of the frontal cortex of developing wistarKey words: MORINGA OLEIFERA; Cerebrum; Frontal Cortex

scholarly journals EFEK PEMBERIAN EKSTRAK KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR YANG DIBERI ASPIRIN

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Janet Walangitan ◽  
Lily Loho ◽  
Meilany Durry
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Cinnamon Extract ◽  
Group A ◽  
Cinnamomum Burmannii ◽  
Group B ◽  
Group D

Abstract: Cinnamon (Cinnamomum burmannii) is a traditional herbal plants which are often found in our daily life and has many benefits especially in health. This study were designed to know the effect of cinnamon on gastric mucosa given aspirin. This was an experimental research and used Wistar rats as the subject research.  The Wistar rats were randomly divided into 6 mice of control group and 9 mice of treatment group. Group A (K-) given pellets, group B (K+) given pellets and aspirin 150 mg/kgBB for 7 days, group C given pellets, aspirin 150 mg/kgBB and cinnamon extract 3 mg for 3 days, group D given pellets, aspirin 150 mg/kgBB for 7 days and given cinnamon extract 3 mg for 3 days, group E given pellets, aspirin 150 mg/kgBB for 7 days and given pellets only (without treatment) for 3 days. The results showed that aspirin cause gastric mucosa damage in group B (K+) compared with kelompok A (K-). Group C and D showed less inflammatory cells compared with group B (K+). Group E showed more inflammatory cells compared with group D. The study suggested that cinnamon extract has protective and therapeutic effects on gastric mucosa of Wistar rats. Keywords: cinnamon, gaster, aspirin.   Abstrak: Kayu manis (Cinnamomum burmannii) merupakan tanaman herbal tradisional yang banyak dijumpai dalam kehidupan sehari- hari dan memiliki banyak manfaat termasuk dalam bidang kesehatan. Penelitian ini bertujuan untuk mengetahui apakah ada efek pemberian kayu manis terhadap mukosa lambung yang diberi aspirin. Desain Penelitian yang digunakan adalah metode eksperimental dan subjek penelitian menggunakan tikus Wistar, yang terbagi atas 6 ekor kontrol dan 9 ekor perlakuan. Kelompok A (K-) diberikan pelet selama 7 hari, kelompok B (K+) diberikan pelet dan aspirin 150mg/kgBB selama 7 hari, kelompok C diberikan pelet, aspirin 150 mg/kgBB, ekstrak kayu manis 3 mg secara bersama- sama selama 7 hari, kelompok D diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian ekstrak kayu manis 3 mg selama 3 hari, kelompok E diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian pelet saja (tanpa perlakuan) selama 3 hari. Hasilnya menunjukkan aspirin menimbulkan kerusakan mukosa lambung pada kelompok B (K+) dibandingkan dengan kelompok A (K-). Kelompok C dan D menunjukkan sel-sel radang yang lebih sedikit dari kelompok B(kontrol +). Kelompok E menunjukkan sel- sel radang yang lebih banyak dibandingkan dengan kelompok D. Kesimpulan dari penelitian ini adalah kayu manis mempunyai efek protektif dan terapeutik terhadap mukosa lambung tikus Wistar. Kata kunci: kayu manis, lambung, aspirin.

scholarly journals Gambaran histopatologik lambung tikus wistar (Rattus norvegicus) yang diberi perasan umbi bengkuang (Pachyrhizus erosus (L) Urban) setelah induksi aspirin

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Debora R. Mentang ◽  
Lily L. Loho ◽  
Poppy M. Lintong
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Pachyrhizus Erosus ◽  
Acute Gastritis ◽  
Experimental Laboratory ◽  
Inflammatory Drugs ◽  
Group B ◽  
Group D

Abstract: Acute gastritis is an acute inflammation of the gastric mucosal and submucosal. This disease is caused by a variety of factors, one of which is the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin. Aspirin is one of the salicylate group that is irritating to the stomach. Yam stimulate the growth of epithelial cells also repair the surface of cells in the gastric mucosal. This research aims to describe histopathologic Wistar rats were given gastric juice yam tubers after induction aspirin. This is an experimental laboratory study conducted in September 2014 until January 2015 with a sample of 20 Wistar rats were divided into 4 groups. A group given no treatment. Group B was given aspirin 150 mg for 10 days. Group C was given aspirin 150 mg for 10 then given yam tuber juice for 3 days. Group D was given aspirin 150 mg for 10 days and then given pellets for 3 days. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: Aspirin showed signs of acute gastritis and giving yam tuber juice of showed inflammation-inflammatory less than without giving juice of yam tubers.Keywords: bengkoang, aspirin, acute gastritisAbstrak : Gastritis akut adalah inflamasi akut pada mukosa dan submukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan obat non steroid anti inflamasi (NSAID) seperti aspirin. Aspirin merupakan salah satu dari golongan salisilat yang bersifat iritatif terhadap lambung. Bengkuang merangsang pertumbuhan sel-sel epitel juga perbaikan permukaan sel pada mukosa lambung. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik lambung tikus wistar yang diberi perasan umbi bengkuang setelah induksi aspirin. Penelitian ini bersifat eksperimental laboratorik yang dilakukan pada bulan September 2014 sampai Januari 2015 dengan sampel 20 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan aspirin 150 mg selama 10 hari. Kelompok C diberikan aspirin 150 mg selama 10 kemudian diberikan perasan umbi bengkuang selama 3 hari. Kelompok D diberikan aspirin 150 mg selama 10 hari kemudian diberikan pelet selama 3 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: Pemberian aspirin menunjukkan tanda-tanda gastritis akut dan pemberian perasan umbi bengkuang menunjukkan radang-radang lebih sedikit dibandingkan tanpa pemberian perasan umbi bengkuang.Kata kunci: bengkuang, aspirin , gastritis akut

scholarly journals Histological Assessment on Cadmium Induced Liver Damage Following Oral Administration of Moringa oleifera Oil and Anarcardium occidentale Nuts Oil in Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
O. D. Omotoso ◽  
S. A. Adelakun ◽  
O. O. Oyewo ◽  
B. T. Adediran
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Central Vein ◽  
Group A ◽  
Moringa Oleifera Oil ◽  
Group B ◽  
Group D

Background: Cadmium is a human carcinogen element and one of the most toxic pollutants in the environment. Present study aims at histomorphological study of some of the effects of Moringa oleifera oil and cashew (Anarcardium occidentale) nuts oil on Cadmium-induced liver damage in wistar rats. Methods: Thirty-five Wistar rats (80-180 g) were randomly selected and divided into seven groups of five rats each after acclimatization for two weeks. Group A which served as control received phosphate buffer, Group B received Cadmium only (3.5 mg/kg body weight). Group C received Vitamin C and Vitamin E; Group D received Vitamin C, Vitamin E and Cadmium. Group E received Cadmium and Moringa oleifera oil, Group F received Cadmium and Cashew nut oil and Group G received Cadmium (3.5 mg/kg body weight) and Moringa oleifera oil and Cashew (Anarcardium occidentale) nut oil. Results: Histological examination revealed normal histo-architecture of the liver, the central vein and the hepatocytes were seen clearly and there was orderly arrangement of the hepatocytes in Group A (control group). In group B, there was enlarged portal vein and there was distorted arrangement of the hepatocytes. In group C, the hepatocytes and the central vein were seen. In group D, the central vein and hepatocytes were seen though not orderly arranged. In groups E, F and G, there was restoration of the hepatocytes and the central vein was seen. Conclusion: This therefore suggests that Moringa oleifera oil and Cashew (Anarcardium occidentale) nut oil have ameliorative effects which led to the restoration of the damaged and distorted hepatocytes.

scholarly journals GAMBARAN HISTOPATOLOGIK HATI TIKUS WISTAR YANG DIINDUKSI CCL4 DAN DIBERI AIR REBUSAN TANAMAN CAKAR AYAM (Selaginella doederleinii Hieron)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Anthonio B. Lengkong
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Control Group ◽  
Histological Features ◽  
Phytochemical Content ◽  
Histopathological Features ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Herbal Plant

Abstract: The plant Selaginella doederleinii is a herbal plant. Its phytochemical content including saponin, flavonoid and alkaloid can function as hepatoprotector. The objective of this study was to reveal the histopathological features of wistar rats induced with CCl4 and administered with water extract of the leaves of Selaginella doederleinii. This study was an experimental research employing 16 wistar rats. The rats were divided into 5 groups. Group A was the control group, whereas the other groups were treated with CCl4 for five days. The differences between these groups were as follows; after treatment with CCl4, rats in group B were terminated, rats in group C were fed with regular pellets, rats in group D were administered with water extract of the leaves of Selaginella doederleinii of 1.6 cc/day, and rats in group E were administered with water extract of  the leaves of Selaginella doederleinii of 3.2 cc/day. Results showed that the histological features of the liver of rats administered with water extract of  the leaves of Selaginella doederleinii for three days after treated with CCl4 exhibit numerous regeneration of liver cells. However, if the administration was prolonged for six days then fatty liver was observed. To conclude, Selaginella doederleinii can improve the histopathological features of the liver of rats induced with CCl4, however if the administration was prolonged then liver damage may occur. Keywords: Selaginella doederleinii, CCl4, histopathological features of the liver of wistar rats.   Abstrak: Tanaman cakar ayam merupakan sediaan herbal. Kandungan yang terdapat didalamnya seperti saponin, flavonoid dan alkaloid berfungsi sebagai hepatoprotektor. Penelitian ini bertujuan untuk melihat gambaran histopatologik hati tikus wistar yang diinduksi CCl4 dan diberi air rebusan tanaman cakar ayam. Penelitian ini merupakan penelitian eksperimental yang menggunakan 16 ekor tikus wistar sebagai sampel. Tikus wistar dibagi dalam 5 kelompok. Kelompok A merupakan kelompok kontrol, sedangkan kelompok lainnya diberi CCl4 selama 5 hari. Perbedaan antara keempat kelompok lainnya yaitu setelah pemberian CCl4 kelompok B langsung diterminasi, kelompok C diberi pakan standart, kelompok D diberi tanaman cakar ayam dosis 1,6 cc/hari, dan kelompok E diberi tanaman cakar ayam dosis 3,2 cc/hari. Kelompok C, D, dan E dilakukan 2 kali terminasi yaitu hari ke-3 dan ke-6. Setelah perlakuan, terminasi dilakukan untuk mengetahui gambaran makroskopis dan mikroskopis hati tikus wistar. Dari hasil pengamatan yang dilakukan, gambaran hati tikus yang diberi air rebusan tanaman cakar ayam selama 3 hari setelah induksi CCl4 menunjukkan banyak regenerasi sel hati, tetapi pada pemberian selama 6 hari gambaran yang terlihat adalah perlemakan hati. Pemberian air rebusan tanaman cakar ayam dapat memperbaiki gambaran histopatologik hati yang diinduksi CCl4dan dalam jangka waktu lebih panjang dapat menyebabkan kerusakan hati. Kata kunci: Tanaman cakar ayam, CCl4, gambaran histopatologik hati tikus wistar.

scholarly journals Gambaran histopatologik hati tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Satriani Syahrin ◽  
Carla Kairupan ◽  
Lily Loho
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Moringa Leaf Extract

Abstract: Moringa (Moringa oleifera) is a traditional plant, which is known to treat liver disease. The effect of moringa is related to the antioxidant activity of its compounds such as quercetin and flavonoid silymarin which are useful as liver protector. Carbon tetraclorida is a hepatotoxic substance that is usually used as an inducer of liver damage in studies related to hepatoprotector activity of a substance. This study aimed to describe liver histopathological features of Wistar rats fed with moringa leaf extract after CCl4 induction. This was an experimental study using 24 Wistar rats (Rattus norvegicus) which were divided into 4 groups. Group A, the negative control, was terminated at day 6th, and the other groups (B, C, and D) were induced with CCl4 0.05 cc/day for 5 days. After CCl4 induction, group B were terminated at day 6th; group C was treated with moringa leaf extract 100 mg/day for 5 days and was terminated at day 11th; group D received no treatment for 5 days and was terminated at day 11th. The results showed that groups induced with CCl4 for 5 days showed the presence of inflammatory cells and fatty cells. The groups treated with moringa leaf extract 100mg/day for 5 days after CCl4 induction 0.05 cc/day exhibited regeneration of liver cells in nearly all lobules. Conclusion: Administration of moringa leaf extract of 100mg/day could accelerate liver cell regeneration of Wistar rats after induction of CCl4 0.05cc/day.Keywords: moringa leaf extract, carbon tetrachloride, histopathologogical image of liverAbstrak: Kelor merupakan tanaman tradisional yang diketahui dapat mengobati penyakit hati. Khasiat obat tanaman kelor dihubungkan dengan kandungan senyawa kimia quercetin dan silymarin golongan flavonoid dengan aktivitas antioksidan yang dapat melindungi dan mengobati kerusakan hati. Karbon tetraklorida merupakan zat hepatotoksik yang lazim dipakai sebagai penginduksi kerusakan hati dalam pengujian aktivitas hepatoprotektor suatu zat. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik hati tikus wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi CCl4. Jenis penelitian ini ialah eksperimental menggunakan 24 ekor tikus Wistar spesies Rattus norvegicus yang dibagi dalam 4 kelompok. Kelompok A merupakan kelompok kontrol negatif, diterminasi pada hari ke-6; kelompok B,C, dan D (kelompok perlakuan) diberi CCl4 dengan dosis 0,05 cc/hari selama 5 hari. Setelah pemberian CCl4, kelompok B langsung diterminasi pada hari ke-6; kelompok C diberi ekstrak daun kelor 100 mg/hari selama 5 hari, diterminasi pada hari ke-11; kelompok D tidak diberi perlakuan selama 5 hari, diterminasi pada hari ke-11. Hasil penelitian menunjukkan bahwa kelompok tikus yang diinduksi CCl4 selama 5 hari secara histopatologik memperlihatkan sel-sel radang dan perlemakan sel. Kelompok tikus yang diberi ekstrak daun kelor 100 mg/hari selama 5 hari setelah diinduksi CCl4 0,05 cc/hari menunjukkan regenerasi sel hati, hampir di seluruh lobuli hati. Simpulan: Pemberian ekstrak daun kelor 100 mg/hari setelah induksi CCl4 0,05 cc/hari dapat mempercepat regenerasi sel hati tikus Wistar yang mengalami cedera akibat CCl4.Kata kunci: ekstrak daun kelor, karbon tetraklorida, gambaran histopatologik hati

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