scholarly journals Perubahan histologik pada usus besar hewan coba postmortem

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Clifford Lapian ◽  
Sunny Wangko ◽  
Djon Wongkar
Keyword(s):  
Cell Death ◽  
Observational Study ◽  
Large Intestine ◽  
Oxygen Supply ◽  
Domestic Pig ◽  
Anaerobic Process ◽  
Intestinal Crypt ◽  
Aerobic Process ◽  
Mucosal Layer ◽  
Short Time

Abstract: Cell death occurs after somatic death. The aerobic process of cells would be halted as a result of somatic death, whereas the anaerobic process will continue. Therefore, the cells do not die in a short time although the oxygen supply has been depleted. This anaerobic process will have an impact on the morphology and activity of cells. This study was aimed to obtain the microscopic changes of large intestine for 24 hours postmortem. This was a descriptive observational study. A domestic pig weighed 20 kg was used as sample. The microscopic examinations were performed at several interval times as follow: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 14, 16, 18, 20, 22, and 24 hours postmortem. The results showed that the microscopic changes of the large intestine were identified the earliest at 2 hours postmortem as congestion with dilatation of the intestinal crypt lumen; lysis of a number of intestinal crypts at 6 hours postmortem; all intestinal crypts were lysis leaving empty areas; and the mucosal layer and intestinal crypts could not be identified at 24 hours postmortem. Conclusion: The earliest microscopic changes of the large intestine occured at 2 hours postmortem. Lysis of the intestinal crypts began at 6 hours postmortem and became complete at 12 hours postmortem. Keywords: large intestine, postmortem, intestinal crypt Abstrak: Kematian sel terjadi setelah kematian somatis. Proses aerobik sel-sel akan terhenti akibat kematian somatis, sedangkan proses anaerobik akan tetap berlangsung. Hal tersebut mengakibatkan sel-sel tidak mati dalam waktu yang singkat meskipun pasokan oksigen telah habis. Proses anaerobik ini akan berdampak pada morfologi dan aktivitas sel. Penelitian ini bertujuan untuk mendapatkan perubahan gambaran histologik usus besar pada hewan coba selama 24 jam postmortem. Jenis penelitian ialah deskriptif observasional. Sampel penelitian ialah satu ekor babi domestik dengan berat 20 kg. Pengamatan mikroskopik terhadap sampel dilakukan pada beberapa interval waktu, yaitu: 0 jam, 1 jam, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 7 jam, 8 jam, 9 jam, 12 jam, 14, 16 jam, 18 jam, 20 jam, 22 jam, dan 24 jam postmortem. Hasil penelitian mendapatkan perubahan mikroskopik postmortem paling awal pada usus besar terjadi pada 2 jam postmortem berupa kongesti dengan lumen kripta Lieberkuhn yang berdilatasi; pada 6 jam postmortem tampak sebagian kripta Lieberkuhn telah lisis; pada 12 jam postmortem seluruh kripta Lieberkuhn telah lisis; dan pada 24 jam postmortem lapisan epitel dan kripta Lieberkuhn tidak dapat diidentifikasi lagi. Simpulan: Perubahan histologik postmortem paling awal pada 3 jam postmortem, lisis kripta Lieberkuhn telah tampak sejak 6 jam postmortem, dan menyeluruh pada 12 jam postmortem.Kata kunci: usus besar, postmortem, kripta Lieberkuhn

scholarly journals Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply

2021 ◽  
Vol 44 (10) ◽  
pp. 1458-1464
Author(s):  
Yuya Mizukami ◽  
Yuki Takahashi ◽  
Kazunori Shimizu ◽  
Satoshi Konishi ◽  
Yoshinobu Takakura ◽  
...  
Keyword(s):  
Cell Death ◽  
Oxygen Supply ◽  
Calcium Peroxide

Development of myocardial infarction

ESC CardioMed ◽  
2018 ◽  
pp. 1230-1232
Author(s):  
Pascal Vranckx
Keyword(s):  
Myocardial Infarction ◽  
Cell Death ◽  
Myocardial Ischaemia ◽  
Oxygen Supply ◽  
Myocardial Cell ◽  
Time Dependent ◽  
Collateral Flow ◽  
Complete Occlusion ◽  
Cell Layers ◽  
Artery Flow

Myocardial infarction is the irreversible myocardial cell death (necrosis) secondary to a prolonged lack of oxygen supply (ischaemia) caused by a complete occlusion of a major coronary in the absence of forward or collateral flow. Within the perfusion area of the occluded artery, flow deprivation and myocardial ischaemia are usually most severe subendocardially (apart from the innermost cell layers nourished from the cavity) and, at least in dogs, cell death progresses from the subendocardium to the subepicardium in a time-dependent fashion.

scholarly journals Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
I. K. Hals ◽  
A. M. Rokstad ◽  
B. L. Strand ◽  
J. Oberholzer ◽  
V. Grill
Keyword(s):  
Cell Death ◽  
Oxygen Consumption ◽  
Beta Cell ◽  
Islet Transplantation ◽  
Oxygen Supply ◽  
Acute Hypoxia ◽  
Human Islets ◽  
Culture Conditions ◽  
Positive Finding ◽  
Potential Use

Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested susceptibility of alginate microencapsulated human islets to experimental hypoxia (0.1–0.3% O2for 8 h, followed by reoxygenation) on viability and functional parameters. Hypoxia reduced viability as measured by MTT by33.8±3.5% in encapsulated and42.9±5.2% in nonencapsulated islets (P<0.2). Nonencapsulated islets released 37.7% (median) more HMGB1 compared to encapsulated islets after hypoxic culture conditions (P<0.001). Glucose-induced insulin release was marginally affected by hypoxia. Basal oxygen consumption was equally reduced in encapsulated and nonencapsulated islets, by22.0±6.1% versus24.8±5.7%. Among 27 tested cytokines/chemokines, hypoxia increased the secretion of IL-6 and IL-8/CXCL8 in both groups of islets, whereas an increase of MCP-1/CCL2 was seen only with nonencapsulated islets.Conclusion. Alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia. This is a positive finding in relation to potential use of encapsulation for islet transplantation.

scholarly journals Dimethyl Sulfoxide Protects Escherichia coli from Rapid Antimicrobial-Mediated Killing

2016 ◽  
Vol 60 (8) ◽  
pp. 5054-5058 ◽  
Author(s):  
Hongfei Mi ◽  
Dai Wang ◽  
Yunxin Xue ◽  
Zhi Zhang ◽  
Jianjun Niu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reactive Oxygen Species ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Dimethyl Sulfoxide ◽  
Oxygen Species ◽  
Content Type ◽  
Antimicrobial Studies ◽  
Ros Accumulation ◽  
Short Time

ABSTRACTThe contribution of reactive oxygen species (ROS) to antimicrobial lethality was examined by treatingEscherichia coliwith dimethyl sulfoxide (DMSO), an antioxidant solvent frequently used in antimicrobial studies. DMSO inhibited killing by ampicillin, kanamycin, and two quinolones and had little effect on MICs. DMSO-mediated protection correlated with decreased ROS accumulation and provided evidence for ROS-mediated programmed cell death. These data support the contribution of ROS to antimicrobial lethality and suggest caution when using DMSO-dissolved antimicrobials for short-time killing assays.

scholarly journals Pengolahan Limbah Cair Pembuatan Minuman Ringan Dengan Reaktor Semiaerobik, TCPS, Reaktor Anaerobic Dua Tahap

2018 ◽  
Vol 5 (1) ◽  
pp. 20 ◽  
Author(s):  
Hasti Suprihatin
Keyword(s):  
Organic Waste ◽  
Waste Treatment ◽  
Soft Drink ◽  
Threshold Value ◽  
Anaerobic Treatment ◽  
First Year ◽  
Anaerobic Process ◽  
Aerobic Process ◽  
Organic Waste Treatment ◽  
Two Stages

The existing soft drink factory can produce liquid organic waste with a COD content starts from 6,000 mg/l to 15,000 mg/l with a discharge by 10 m3/day to 100 m3/day. The objective of the research is to obtain organic waste processing equipment that produces processing that meets the threshold value. The research outcomes are as a reference for the industry that requires a representative organic waste treatment unit.The research is divided into two stages in two years. The first year of the research is semi-aerobic and anaerobic process, then for second year is aerobic process research, semi-aerobic process, anaerobic process and aerobic process. The 12 hours HRT process at the first run resulted in a COD concentration of 12,000 mg/l to 8,765 mg/l directly entering an anaerobic I and out 4,640 mg/l and entering anaerobic II reactor and exiting with COD 1,380 mg/l. Decrease percentage of total COD (12,000 – 1,380) x 100 /12,000 = 88.5%. The 18 hours HRT process at the first run resulted in a COD concentration by 12,000 mg/l to 8,665 mg/l entering the anaerobic reactor I and out 4,125 mg/l and entering the anaerobic II and out with COD 965 mg/l. Decrease in total COD (12,000 - 965) x 100 / 12,000 = 91.95%.From the experimental stage of semi-aerobic step-screening-anaerobic treatment of two-stage liquid liquor soft drink obtained COD concentration of 12,000 mg/l can be reduced concentration to the threshold specified with removal of 88.5% - 91.95%.

Development of myocardial infarction

ESC CardioMed ◽  
2018 ◽  
pp. 1230-1232
Author(s):  
Pascal Vranckx
Keyword(s):  
Myocardial Infarction ◽  
Cell Death ◽  
Myocardial Ischaemia ◽  
Oxygen Supply ◽  
Myocardial Cell ◽  
Time Dependent ◽  
Collateral Flow ◽  
Complete Occlusion ◽  
Cell Layers ◽  
Artery Flow

Myocardial infarction is the irreversible myocardial cell death (necrosis) secondary to a prolonged lack of oxygen supply (ischaemia) caused by a complete occlusion of a major coronary in the absence of forward or collateral flow. Within the perfusion area of the occluded artery, flow deprivation and myocardial ischaemia are usually most severe subendocardially (apart from the innermost cell layers nourished from the cavity) and, at least in dogs, cell death progresses from the subendocardium to the subepicardium in a time-dependent fashion.

Why is it worth testing the ability of zinc to protect against ischaemia reperfusion injury for human application

Metallomics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1330-1343 ◽  
Author(s):  
Joseph Ischia ◽  
Damien M. Bolton ◽  
Oneel Patel
Keyword(s):  
Cell Death ◽  
Reperfusion Injury ◽  
Organ Dysfunction ◽  
Oxygen Supply ◽  
Tissue Injury ◽  
Blood Oxygen ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion

Ischaemia (interruption in the blood/oxygen supply) and subsequent damage induced by reperfusion (restoration of blood/oxygen supply) ultimately leads to cell death, tissue injury and permanent organ dysfunction.

CHEMICAL DETERMINATION OF OESTROGEN DISTRIBUTION IN THE FOETUS AND PLACENTA OF THE DOMESTIC PIG

1974 ◽  
Vol 77 (1) ◽  
pp. 171-185 ◽  
Author(s):  
C. H. Choong ◽  
J. I. Raeside
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Foetal Heart ◽  
Domestic Pig ◽  
Male And Female ◽  
Chemical Determination ◽  
Wet Weight ◽  
The Mean ◽  
Late Stages

ABSTRACT The concentrations of oestrogens and solvolyzable oestrogen conjugates have been estimated chemically in extracts of different foetal organs and of placental tissues of the domestic pig during late stages of pregnancy. Unconjugated oestrogens were not detected (< 0.5 μg/100 g wet weight of tissues) in foetal adrenals, ovaries and testes, nor in foetal spleen. Oestrogen concentrations were low in foetal heart. Foetal placenta, maternal placenta and uterine tissues were analyzed separately. There was a higher oestrogen concentration in foetal (176.3 μg/100 g) than in maternal (74.0 μg/100 g) placenta. In placental tissues oestrogens were present mainly as unconjugated steroids while a greater proportion was found as conjugates in uterine tissues. The mean concentrations of unconjugated oestrogens and oestrogen sulphates respectively were 111.9 and 66.5 μg/100 g, for foetal placenta; 57.9 and 16.5 μg/100 g, for maternal placenta; and 3.1 and 7.1 μg/100 g, for uterine tissues. Greater oestrogen concentrations were noted in the foetal placenta and fluids from males than from females; the total oestrogen concentrations for male and female samples respectively were 235.5 and 121.2 μg/100 g, for foetal placenta; and 815.7 and 454.7 μg/100 ml, for foetal fluids. Oestrogens were present mostly as conjugates in various foetal tissues. The mean oestrogen concentrations for unconjugated and solvolyzable forms respectively were 45.2 and 80.9 μg/100 g, for liver; 26.0 and 42.4 μg/100 g, for kidneys; 14.6 and 38.5 μg/100 g, for lungs; and 10.9 and 29.4 μg/100 g, for skin. Unconjugated oestrogens predominated, however, in intestinal tissues and meconium. Mean concentrations of unconjugated and conjugated oestrogens respectively were 256.1 and 98.2 μg/100 g, for small intestine; 531.8 and 132.1 μg/100 g, for large intestine; and 2152.2 and 137.2 μg/100 g, for meconium. Oestrone was the major oestrogen found. Lesser amounts of oestradiol-17β were present.

scholarly journals Clinical course of IPF in Italian patients during 12 months of observation: results from the FIBRONET observational study

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
V. Poletti ◽  
◽  
C. Vancheri ◽  
C. Albera ◽  
S. Harari ◽  
...  
Keyword(s):  
Lung Function ◽  
Observational Study ◽  
Clinical Course ◽  
Real Life ◽  
Primary Objective ◽  
Course Of Disease ◽  
Antifibrotic Therapy ◽  
Short Time ◽  
Mean Time

Abstract Background FIBRONET was an observational, multicentre, prospective cohort study investigating the baseline characteristics, clinical course of disease and use of antifibrotic treatment in Italian patients with idiopathic pulmonary fibrosis (IPF). Methods Patients aged ≥ 40 years diagnosed with IPF within the previous 3 months at 20 Italian centres were consecutively enrolled and followed up for 12 months, with evaluations at 3, 6, 9 and 12 months. The primary objective was to describe the clinical course of IPF over 12 months of follow-up, including changes in lung function measured by % predicted forced vital capacity (FVC% predicted). Results 209 patients (82.3% male, mean age 69.54 ± 7.43 years) were enrolled. Mean FVC% predicted was relatively preserved at baseline (80.01%). The mean time between IPF diagnosis and initiation of antifibrotic therapy was 6.38 weeks; 72.3% of patients received antifibrotic therapy within the first 3 months of follow-up, and 83.9% within 12 months of follow-up. Mean FVC% predicted was 80.0% at baseline and 82.2% at 12 months, and 47.4% of patients remained stable (i.e. had no disease progression) in terms of FVC% predicted during the study. Conclusions FIBRONET is the first prospective, real-life, observational study of patients with IPF in Italy. The short time between diagnosis and initiation of antifibrotic therapy, and the stable lung function between baseline and 12 months, suggest that early diagnosis and prompt initiation of antifibrotic therapy may preserve lung function in patients with IPF. Trial registration: NCT02803580

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