scholarly journals Isolasi dan Identifikasi Bakteri Resisten Merkuri pada Plak Gigi dan Urin

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Priscilla R. Lumanto ◽  
Aaltje Manampiring ◽  
Fona Budiarso
Keyword(s):  
Dental Plaque ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Microbiology Laboratory ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Metal Compounds ◽  
Pharmacy Department ◽  
Cross Sectional Design ◽  
Mercury Resistant Bacteria

Abstract: Mercury toxic effects could be reduced by the presence of mercury resistant microorganisms, such as mercury resistant bacteria. These bacteria possess mercury resistant gene, the operon mere gene. Resistance could also occur through genes that code mercury resistant characteristics and of other heavy metal compounds that generally located within the plasmid. This study was aimed to obtain the profile of bacteria in dental plaque and urine that were resistant to mercury. This was a descriptive prospective study with a cross-sectional design. Isolation and identification of the bacteria were conducted at Microbiology Laboratory of Pharmacy Department, Sam Ratulangi University. The results obtained bacteria of Bacillus sp. which were sensitive to antibiotics ciprofloxacin, erythromycin, and doxycyclin. Conclusion: Mercury resistent bacteria in dental plaque and urine were sensitive to antibiotics ciprofloxacin, erythromycin, and doxycyclin.Keywords: mercury resistent bacteria, dental plaque, urine Abstrak: Toksisitas merkuri dapat berkurang oleh adanya mikroorganisme resisten merkuri, misalnya bakteri resisten merkuri. Hal ini terjadi karena bakteri memiliki gen yang resisten terhadap merkuri, yaitu gen operon mer. Resistensi juga dapat melalui gen yang mengkode sifat resisten terhadap merkuri dan senyawa logam berat lainnya yang umumnya terletak pada plasmid. Penelitian ini bertujuan untuk mengetahui gambaran bakteri dari plak gigi dan urin yang resistensi terhadap merkuri. Jenis penelitian ialah deskriptif prospektif dengan desain potong lintang. Isolasi dan identifikasi bakteri dari plak gigi dan urin yang resisten terhadap merkuri dilakukan di Laboratorium Mikrobiologi Farmasi FMIPA Unsrat. Hasil penelitian mendapatkan bakteri dari sampel gigi dan urine ialah bakteri Bacillus sp. yang sensitif terhadap antibiotik siprofloksasin, eritromisin, dan doksisiklin. Simpulan: Bakteri resisten terhadap merkuri dalam sampel plak gigi dan urin ialah Bacillus sp. yang sensitif terhadap antibiotik siprofloksasin, eritromisin, dan doksisiklin.Kata kunci: bakteri resisten merkuri, plak gigi, urin

scholarly journals UJI RESISTENSI BAKTERI BACILLUS SP YANG DIISOLASI DARI PLAK GIGI TERHADAP MERKURI DAN ERITROMISIN

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Terence Kanzil ◽  
Fatimawali . ◽  
Aaltje Manampiring
Keyword(s):  
Heavy Metal ◽  
Toxic Effects ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Gram Positive ◽  
Toxic Heavy Metal ◽  
Mer Operon ◽  
Dental Fillings ◽  
Gram Positive Bacteria ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a toxic heavy metal that is used for dental fillings in the form of amalgam. To reduce the toxic effects produced by mercury, mercury resistant bacteria can be used. Bacillus sp is a gram-positive bacteria that is resistant to mercury. Besides having the mer operon genes that can transform Hg2+ to Hg0 that is less toxic, Bacillus sp bacteria also produce esterase that cause these bacteria resistant to erythromycin antibiotic. Erythromycin is a macrolide class of antibiotic used for the treatment of diseases caused by Gram-positive bacteria, especially Staphylococcus and Diphtheroids. To determine the resistance of Bacillus sp bacteria against mercury and erythromycin antibiotic. This study used a descriptive exploratory method with samples of bacteria and mercury are already available in the Laboratory of Pharmaceutical Microbiology. Based on the research that has been conducted, showed that the Bacillus sp bacteria is resistant to mercury and erythromycin.Keywords: bacteria, bacillus sp, resistant, mercury, erythromycinAbstrak: Merkuri merupakan logam berat bersifat toksik yang digunakan untuk penambalan gigi dalam bentuk amalgam. Untuk mengurangi efek toksik yang dihasilkan oleh merkuri, dapat digunakan bakteri resisten merkuri. Bakteri Bacillus sp merupakan bakteri gram positif yang resisten terhadap merkuri. Selain memiliki gen mer operon yang dapat mengubah Hg2+ menjadi Hg0 yang kurang toksik, bakteri Bacillus sp juga membentuk esterase yang menyebabkan terjadinya resisten bakteri ini terhadap antibiotik eritromisin. Eritromisin adalah antibiotik golongan makrolid yang digunakan untuk pengobatan penyakit akibat bakteri Gram positif khususnya Staphylococcus dan Diphtheroids. Untuk mengetahui resistensi bakteri Bacillus sp terhadap merkuri dan antibiotik eritromisin. Penelitian ini menggunakan metode deskriptif eksploratif dengan sampel bakteri dan merkuri yang sudah tersedia di Laboratorium Mikrobiologi Farmasi. Berdasarkan hasil penelitian yang telah dilakukan, diperoleh bahwa bakteri Bacillus sp resisten terhadap merkuri dan eritromisin.Kata Kunci: bakteri, bacillus sp, resisten, merkuri, eritromisin

scholarly journals Pengelolaan Obat Dengan E-Purchasing Untuk Pasien Program Rujuk Balik Di Fasilitas Kesehatan Tingkat Pertama

2019 ◽  
Vol 22 (2) ◽  
pp. 99-105
Author(s):  
Herti Maryani ◽  
Lusi Kristiana ◽  
Pramita Andarwati ◽  
Astridya Paramita ◽  
Ira Ummu Aimanah
Keyword(s):  
Data Collection ◽  
Health Service ◽  
Study Drug ◽  
Drug Management ◽  
Design Data ◽  
Cross Sectional ◽  
Descriptive Research ◽  
Pharmacy Department ◽  
Cross Sectional Design ◽  
Depth Interviews

  PRB is a health service provided to people with chronic diseases. The implementation of PRB has been runningsince 2014, but until now it is still not optimal, one of which is the procurement and availability of medicines. The aim of the study was to study drug management for PRB patients. The research was conducted in Surabaya 2018. This is descriptive research with cross-sectional design. Data collection by in-depth interviews with pharmacy department managers in two FKTP units and pharmacies in Surabaya. Data were analyzed descriptively. The results of the study show that FKTP doesn’t buy medicine with e-purchasing, because the drug is given by the pharmacy according to the BPJS mapping list. The pharmacy has many obstacles to ordering drugs with e-purchase, so the order is done conventionally. The pharmacy orders drugs in several ways using the Order Letter, calling PBF and ordering via the WhatsApp (WA) application. The Guidelines for Procurement of Medicines with E-Purchasing Procedures Based on E-Catalogs already exist, but socialization must continue to be carried out, especially at the level of Puskesmas and pharmacies. Periodic evaluations must be carried out so that problems and defi ciencies that occur in the fi eld can be immediately resolved.Cooperation and good intentions are needed between various parties so that all involved can benefi t from this program, especially PRB patients. Abstrak Program Rujuk Balik (PRB) adalah pelayanan kesehatan yang diberikan kepada penderita penyakit kronis. PRBsudah berjalan sejak tahun 2014, namun masih belum optimal, salah satunya perihal pengelolaan obat. Tujuan penelitian adalah mengkaji pengelolaan obat untuk pasien PRB. Penelitian dilakukan di Surabaya tahun 2018. Jenis penelitian adalah deskriptif dengan desain potong lintang. Pengumpulan data dilakukan dengan wawancara mendalam kepada pengelola bagian farmasi di 2 unit Puskesmas dan 2 Apotek di Surabaya. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa Puskesmas tidak melakukan pengadaan obat secara e-purchasing, karena obat diberikan oleh apotek sesuai daftar mapping BPJS. Apotek mempunyai banyak kendala dalam melakukan pemesanan obat dengan e-purchase, sehingga pemesanan dilakukan secara konvensional. Apotek melakukan pemesanan obat dengan beberapa cara yaitu menggunakan Surat Pemesanan (SP), menelpon PBF (Perusahaan Besar Farmasi) dan melalui aplikasi WhatsApp (WA).Petunjuk pelaksanaan pengadaan obat dengan prosedur E-Purchasing, berdasarkan E-Catalogue, sudah ada namun sosialisasi harus terus dilakukan terutama di tingkat Puskesmas dan apotek. Evaluasi berkala harus dilakukan agar permasalahan dan kekurangan yang terjadi di lapangan dapat segera diselesaikan. Perlunya kerja sama dan komitmen antar berbagai pihak sehingga semua yang terlibat dapat merasakan manfaat akan program ini, terutama pasien PRB.

scholarly journals Molecular Detection of blaTEM, blaCTX-M and blaSHV Genes in Extended Spectrum β-Lactamase (ESBL) Escherichia Coli from Clinical Samples

2021 ◽  
Author(s):  
Mahesh Kumar Chaudhary ◽  
Indrani Jadhav ◽  
Megha Raj Banjara
Keyword(s):  
Escherichia Coli ◽  
Major Gene ◽  
Polymyxin B ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Microbiology Laboratory ◽  
Cross Sectional ◽  
Extended Spectrum

Abstract BackgroundExtended spectrum β-lactamases are the group of beta-lactamase enzymes which confer resistance to the oxyimino-cephalosporins and monobactams. Emergence of ESBL producing genes which possess a serious threat for the treatment of infections both in community and hospitals since it is found to be increasing trends of multidrug resistance. This study was focused to find out the ESBLs producing genes.MethodsThis was a cross-sectional study conducted over a period of 2 years (September 2018 to April 2020) at microbiology laboratory of Nepal Mediciti Hospital. Clinical samples were processed in microbiology laboratory and culture isolates were indetified and characterized by standard microbiological techniques. following standard procedures.Antibiotic susceptibility testing was performed by modified Kirby-Bauer disc diffusion method as recommended by Clinical and Laboratory Standard Institute. Extended spectrum beta-lactamases were phenotypically confirmed by combined disc method.ESBL producing genes i.e. blaTEM,blaCTX-M and blaSHV were confirmed by PCR.ResultsOf the 1449 total E.coli isolates, 323/1449(22.29%) isolates were multi -drug resistance.Among total MDR Escherichia coli isolates, 215/323(66.56%) isolates were ESBL producers. The maximum number of ESBL Escherichia coli was isolated from urine 194(90.23%), followed by sputum 12(5.58%), swab 5 (2.32%), pus 2 (0.93%) and blood 2 (0.93%).Antibiotic susceptibility pattern of ESBL E.coli producers showed highest sensitivity towards tigecycline (100%) followed by polymyxin b, colistin and meropenem.Out of 215 phenotypically confirmed ESBL E.coli,only 186(86.51%) isolates were found to positive by PCR.The last 29(13.49%) were negative for any of the resistant genes.Among the ESBL genotypes,most common was blaTEM 118(63.4%) followed by blaCTX-M 68(36.6%).ConclusionThe emergence of MDR and ESBL producing E.coli isolates with high antibiotic resistant rates to commonly used antibiotics and increased predominance of major gene types blaTEM is a serious concern to the clinicians as well as microbiologist. This study forwarded a real massage to all the clinicians for the emergence of XDR and PDR resistant bacteria and preservation of antibiotics for their proper use in near future, if past experience with MDR and ESBLs is any indicator.

scholarly journals IDENTIFIKASI BAKTERI RESISTEN MERKURI PADA KARANG GIGI, URIN DAN FESES PADA INDIVIDU KELURAHAN PAKADOODAN KOTA BITUNG

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Maichel Yorgen
Keyword(s):  
Research Result ◽  
Clinical Manifestations ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Resistance Level ◽  
The Individual ◽  
Mercury Detoxification ◽  
Bacteria Resistance ◽  
Mercury Resistant Bacteria ◽  
Phenyl Mercury

Abstract: Mercury is one of kinds of hazardous metals which is highly found in nature and spread in rocklike, ore, soil, water, and air as the inorganic and organic compounds. The metal of mercury is dangerous to human’s life because it can cause the substantially clinical manifestations started from being poisoned, nerve palsy and even cause death. One of attempts to mercury detoxification can be done by using mercury-resistant microorganism such as mercury-resistant bacteria. That would happen if the mercury spread inside the human’s body with small value in a very chronic time can make the bacteria inside the human’s body to be able to adapt even able to reduce mercury so that it becomes harmless. The research aims to identify the kind of bacteria that resists to mercury, in this case, to the kind of mercury HgCl2 and Phenyl Mercury found on tartar, urine, and feses upon the individual in Pakadoodan Village of Bitung City by using the way to check the value of bacteria resistance to mercury, afterwards the Physiology test, Biochemical, and Morphology is done to identify the kind of that bacteria. From the research result done there are 4 genus of bacteria which was successfully identified, those are, Streptococcus sp, E.Coli, Bacillus sp, and Staphylococcus sp. By the classification HgCl2 is the bacteria with the genus of     Bacillus sp which is found in urine, feses and tartar, and Staphylococcus sp which is found also in urine with the resistance level of 40 ppm each and phenyl mercury is the bacteria with the genus of Streptococcus sp, which is found in tartar and E.Coli which is found in feses and urine with the resistance level 20 ppm each. Keywords: mercury, bacteria, mercury-resistant bacteria Abstrak: Merkuri adalah salah satu jenis logam berbahaya yang banyak ditemukan di alam dan tersebar dalam batu - batuan, biji tambang, tanah, air dan udara sebagai senyawa anorganik dan organik. Logam merkuri sangat berbahaya terhadap kehidupan manusia karena dapat memberikan manifestasi klinik yang cukup bermakna mulai dari keracunan, kelumpuhan saraf bahkan dapat meneyebabkan kematian. Salah satu usaha untuk detoksifikasi merkuri dapat dilakukan dengan menggunakan mikroorganisme resisten merkuri seperti bakteri resisten merkuri. Hal itu dapat terjadi jika merkuri yang terpapar dalam tubuh manusia dengan kadar yang kecil dalam waktu yang sangat kronis dapat membuat bakteri dalam tubuh manusia tersebut dapat beradaptasi bahkan dapat mampu meruduksi merkuri tersebut sehingga menjadi tidak berbahaya. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri yang resisten terhadap merkuri dalam hal ini terhadap jenis merkuri HgCl2 dan fenil merkuri yang terdapat pada karang gigi, urin dan feses pada individu kelurahan Pakadoodan Kota Bitung dengan cara memeriksa kadar resisten bakteri tersebut terhadap merkuri, setelah itu dilakukan uji fisiologi, uji biokimia dan uji morfolgi untuk mengidentifikasi jenis bakteri tersebut.  Dari hasil penelitian yang dilakukan terdapat 4 genus bakteri yang berhasil diidentifikasi yaitu Streptococcus sp, E.coli, Bacillus sp, dan Staphylococcus sp. Dengan klasifikasi HgCl2 adalah bakteri dengan genus Bacillus sp yang terdapat pada urin, feses dan karang gigi, dan Staphylococcus sp yang terdapat juga pada urin dengan tingkat resistensi masing-masing 40 ppm dan fenil merkuri adalah bakteri dengan genus Streptococcus sp, yang terdapat pada karang gigi dan E.coli yang terdapat pada feses dan urin dengan tingkat resistensi masing-masing 20 ppm. Kata kunci: merkuri, bakteri, bakteri resisten merkuri

scholarly journals Differences of Preparation Examination Methods for the Number of Acid-Resistant Bacteria

2020 ◽  
Vol 18 (1) ◽  
pp. 59-67
Author(s):  
Sherly Dewu ◽  
Rahmahsari Bala ◽  
Norma Tiku Kambuno
Keyword(s):  
Infectious Disease ◽  
Infected Patient ◽  
Resistant Bacteria ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Examination Methods ◽  
Cross Sectional Design ◽  
June July ◽  
Number Of Bacteria

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis which transmission is sputum less micro-discharge issued by an infected patient. The examination of tuberculosis in acid-resistant bacteria is recommended by WHO using the Ziehl Neelsen method. The objective of this study is to determine the differences in the way the preparations were examined for the number of acid-resistant bacteria. This research is a cross-sectional design laboratory experimental research. The study was conducted in June-July 2018. The examination was conducted microscopically using the Ziehl Neelsen method. The inspection procedure is performed on direct preparations and indirectly checked. The results of the study conducted showed that there was no significant difference in the number of acid-resistant bacteria (p-value = 0.758). It was concluded that the results of examining the number of positive acid-resistant bacteria in direct and indirect preparations examined did not affect the results of the number of bacteria on the microscopic examination of positive acid-resistant bacteria. It is recommended for further studies on the use of uniformed samples for the making of preparations using one of the samples at any time.

scholarly journals Identifikasi Bakteri Resisten Merkuri Menggunakan Metode 16SrRNA terhadap Plak Gigi pada Pasien Pengguna Tumpatan Amalgam

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Christofel A.N. Tanumihardja ◽  
Billy Kepel ◽  
Widhi Bodhi
Keyword(s):  
Bacillus Thuringiensis ◽  
Dental Plaque ◽  
Resistant Bacteria ◽  
Dental Fillings ◽  
Dental Filling ◽  
Mercury Reductase ◽  
Dental Plaques ◽  
Mercury Resistant Bacteria ◽  
Mathematics And Science ◽  
Amalgam Fillings

Abstract: Amalgam is a popular dental filling due to its cheaper price than other dental fillings. Basically, amalgam is an alloy, consists of two or more metals; one of them is mercury. The unfavorable thing about this alloy is that its vapor in the oral cavity can trigger the development of mercury-resistant bacteria. This type of bacteria has an enzyme called mercury reductase that can reduce Hg2+ to Hg0. 16SsRNA is a gene that contains important information to describe the prokaryotic type. This study was aimed to identify the type of mercury-resistant bacteria from dental plaque of patients with amalgam fillings. Samples were taken from the dental plaques. Isolation of DNA, sequensing of 16SsRNA gene by using PCR, and online BLAST through GenBank NCBI, and finally looking for the closest relative using a phylogenetic tree were performed in the Pharmacy Laboratory, Faculty of Mathematics and Science. The result of BLAST showed 4 types of bacteria, and the closest relative is B. thuringiensis. Conclusion: The type of mercury-resistant bacteria found in dental plaques was Bacillus thuringiensis.Keyword: amalgam, mercury resistant bacteria, dental plaques, 16SsRNA, PCR Abstrak: Amalgam adalah suatu logam campuran yang terdiri dari dua atau beberapa logam yang salah satunya adalah merkuri atau air raksa. Amalgam sebagai bahan tumpatan sampai saat ini masih banyak digunakan oleh dokter gigi karena harganya yang relatif murah. Namun penggunaan amalgam ini dapat melepaskan uap merkuri selama berada di dalam rongga mulut. Penggunaan amalgam ini memicu munculnya bakteri yang resisten terhadap merkuri dimana bakteri dapat mereduksi Hg2+ menjadi Hg0 melalui enzim yang menginduksi merkuri reduktase. 16SsRNA adalah gen yang menampung informasi-informasi penting agar mendeskripsikan jenis-jenis prokariotik. Penelitian ini bertujuan untuk mengidentifikasi bakteri resisten merkuri pada plak gigi pasien pengguna tumpatan amalgam menggunakan metode PCR. Jenis penelitian ialah deskriptif observatif. Sampel diambil dari plak gigi pasien pengguna tumpatan amalgam di Laboratorium Farmasi Fakultas MIPA. Dilakukan langkah-langkah untuk isolasi DNA, sekuensing gen 16SsRNA menggunakan PCR, kemudian dilakukan BLAST secara online melalui GenBank NCBI lalu dicari kekerabatannya menggunakan pohon filogenetik. Hasil BLAST mendapatkan 4 jenis bakteri, dan kekerabatan terdekatnya ialah Bacillus thuringiensis. Simpulan: Jenis bakteri resisten terhadap merkuri pada plak gigi ialah Bacillus thuringiensis.Kata kunci: amalgam, bakteri resisten merkuri, plak gigi, 16SsRNA, PCR

scholarly journals Identifikasi Bakteri dengan Pewarnaan Gram pada Penderita Infeksi Mata Luar di Rumah Sakit Mata Kota Manado

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Trijeri Bulele ◽  
Fredine E. S. Rares ◽  
John Porotu'o
Keyword(s):  
Bacterial Culture ◽  
Resistant Bacteria ◽  
Ocular Infection ◽  
Gram Positive ◽  
Cross Sectional ◽  
Antibiotic Resistant ◽  
Gram Positive Bacteria ◽  
Gram Staining ◽  
Ocular Infections ◽  
Cross Sectional Design

Abstract: Bacteria are the main cause of external ocular infection worldwide. Therefore, it is necessary to improve the treatment according to the cause of the infection in order to prevent the emergence of antibiotic-resistant bacteria. This study was aimed to identify the bacteria by using the Gram staining method in patients with external ocular infections. This was a descriptive study with a cross sectional design using bacterial culture of purulent secretion obtained from conjunctival and palpebral swabs in patients with external ocular infections at Manado Eye Hospital Manado. Based on the types of external ocular infections, conjunctivitis was the leading cause (50%), followed by keratitis (30%), blepharitis (13.3%), and keratoconjunctivitis (6.7%). Females were more frequeny than males (63.3% vs 36.7%). The youngest age in this study was 9 years and the oldest was 81 years, meanwhile, the most common age group was adults >40 years. The most common type of occupation was housewives. The results of bacterial culture were as follows: 19 samples showed no growth; 6 samples (54.5%) of Gram-positive bacteria in the form of staphylococcus, coccus, diplococcus; 3 samples (27.3%) of Gram-negative bacteria in the form of bacillus; and 2 samples (18.2%) of mixed Gram positive and negative bacteria in the form of bacillus and coccus. Conclusion: Gram-positive bacterium in the form of coccus is the most common cause of external ocular infection.Keywords: external ocular infection, bacteria, Gram staining Abstrak: Bakteri ialah penyebab utama infeksi mata luar di seluruh dunia. Pengobatan yang sesuai dengan penyebab infeksi dapat mencegah munculnya bakteri yang resistan terhadap antibiotik. Penelitian ini bertujuan untuk mengidentifikasi bakteri dengan menggunakan metode pewarnaan Gram pada penderita infeksi mata luar. Jenis penelitian ialah deskriptif dengan desain potong lintang menggunakan kultur bakteri hasil swab sekret purulen dari konjungtiva dan palpebra pada penderita infeksi mata luar di Rumah Sakit Mata Kota Manado. Hasil penelitian mendapatkan bahwa jenis infeksi mata luar terbanyak ialah konjungtivitis (50%), diikuti oleh keratitis (30%), blefaritis (13,3%), dan keratokonjungtivitis (6,7%). Perempuan lebih banyak (63,3%) menderita infeksi mata luar dibandingkan laki-laki (36,7%). Usia termuda ialah 9 tahun dan tertua 81 tahun. Kelompok usia terbanyak ialah dewasa >40 tahun. Jenis pekerjaan ibu rumah tangga yang terbanyak dibandingkan dengan pekerjaan lainnya. Hasil proses kultur mendapatkan 19 sampel tidak menunjukan adanya pertumbuhan, 6 sampel (54,5%) bakteri Gram positif dengan bentuk staphylococcus, coccus, diplococcus, 3 sampel (27,3%) bakteri Gram negatif dengan bentuk bacillus, dan 2 sampel (18.2%) campuran bakteri Gram positif dan negatif dengan bentuk bacillus dan coccus. Simpulan: Bakteri Gram positif berbentuk coccus yang paling sering menjadi penyebab infeksi mata luar.Kata kunci: infeksi mata luar, bakteri, pewarnaan Gram

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI

2011 ◽  
Vol 15 (1) ◽  
pp. 282 ◽  
Author(s):  
Fatimawali Fatimawali ◽  
Fatmawaty Badaruddin ◽  
Irawan Yusuf
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pure Culture ◽  
Culture Media ◽  
Inorganic Mercury ◽  
River Estuary ◽  
Nutrient Broth ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mercury Resistant Bacteria

ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI Fatimawali1), Fatmawaty Badaruddin2), Irawan Yusuf 2) 1)Dosen Fakultas  Kedokteran Unsrat Manado dan Mahasiswa Program Doktor Fakultas Kedokteran Universitas Hasanuddin Makassar; 2)Dosen Fakultas Kedokteran Universitas Hasanuddin Makassar ABSTRAK Merkuri adalah logam berat yang bersifat sangat toksik, mempunyai afinitas terhadap gugus thiol protein.  Keberadaan merkuri  dilingkungan, karena secara alamiah maupun karena aktifitas antrofogenik. Di Kabupaten Bolaang Mongondow terdapat beberapa penambang emas rakyat yang menggunakan merkuri untuk mengekstrak emas dari batuan atau ore dan membuang limbah merkuri secara bebas ke lingkungan dan terbawa air hujan sampai kepada perairan.  Sedimen perairan yang terkontaminasi merkuri dapat ditumbuhi bakteri resisten merkuri.  Bakteri resisten merkuri anorhanik dapat digunakan untuk bioremediasi perairan, karena bakteri tersebut mampu mereduksi merkuri anorganik menjadi logam merkuri yang mudah menguap dan kurangtoksik.  Dan merupakan sumber pencemaran merkuri.  Bakteri resisten merkuri mempunyai operon mer yang biasanya terkandung dalam plasmid.  Penelitian ini bertujuan untuk mengisolasi bakteri resisten merkuri anorganik, dan menganalisis kemampuannya dalam mereduksi HgCl2 dalam media nutrient broth.  Bakteri resisten merkuri tinggi, dilakukan analisis Gen 16S rRNAnya untuk mengetahuis pesiesnya.  Hasil penelitian menunjukkan bahwa isolat A1.1.1 merupakan bakteri spesies Klebsiella pneumoniae, mampu mereduksi HgCl2 75% dalam waktu 1 jam, 92% dalam waktu 12 jam dan 99,4%  dalam waktu 24 jam Kata Kunci: bakteri, detoksifikasi,  HgCl2,  Klebsiella pneumoniae   ISOLATION  AND  IDENTIFICATION OF MERCURY-RESISTANT BACTERIUM FROM SARIO RIVER ESTUARY THAT CAN BE USED TO DETOXIFY INORGANIK MERCURY WASTES ABSTRACT Mercury (Hg) is well known for its high toxicity and strong affinity toward the thiol group of proteins. When Hg released into the environment in substantial quantities through natural events and anthropogenic activities . In District of Bolaang Mongondow, many miners who are a source of mercury pollution of aquatic environments to which they use to extract gold from the rock or ore. Waters sediment contaminated with mercury, can be overgrown with microorganism such as bacteria that are resistant to mercury.  Mercury-resistant bacteria can be used for bioremediation of waters due to the ability of these bacteria to reduce toxic inorganic mercury into mercury metal which is volatile and less toxic. Mercury-resistant bacteria has mer Operon which usually contained in the plasmid.  The aim of this study was the selection of bacterial strains resistant to inorganic mercury, as well as to show their capacity to reduce mercury  in pure culture media Nutrient Broth. Twelve isolate bacteria was selected for its capacity to reduce mercury HgCl2 in culture media nutrient broth. The one bacterial strains belong to the spesies Klebsiella pneumoniae. Inoculated in pure culture, these strain showed a mercury reduction of 75% in 1 hour, 92% in 12 hours, and  99,4% in 24 hours. Keywords : bacteria , detoxification, HgCl2 ,  Klebsiella pneumoniae

scholarly journals Isolasi bakteri resisten merkuri pada urin pasien dengan tumpatan amalgam di Puskesmas Tuminting

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Julia V.F. Bahter ◽  
Billy J. Kepel ◽  
Fatimawali .
Keyword(s):  
Exploratory Study ◽  
Human Life ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Dental Clinic ◽  
Continuous Release ◽  
Primary Health ◽  
Toxicity Level ◽  
Mercury Resistant Bacteria ◽  
Amalgam Fillings

Abstract: Metal is very important for human life, albeit, some of them have toxic effects. Mercury is a heavy metal with a high toxicity level. In the field of dentistry, mercury is used as an ingredient of amalgam. The use of amalgam apparently triggers resistant bacteria due to continuous release of mercury since the usage of condensing amalgam in the tooth. This study was aimed to determine whether there were mercury-resistant bacteria in the urine of patients who had amalgam-filling tooth at Puskesmas Tuminting (primary health care) and to identify the types of mercury-resistant bacteria. This was a descriptive exploratory study using urine samples of patients in dental clinic with amalgam fillings minimal for 6 months. The results obtained 30 isolates of mercury-resistant bacteria with 6 genera of bacteria resistant to mercury, as follows: Klebsiella sp, Bacillus sp, Staphylococcus sp, Hafnia sp, Enterobacter sp, and Eubacteria sp. Conclusion: There were mercury-resistant bacteria in the urine of patients with amalgam-filling teeth in the Dental Clinic of Puskesmas Tuminting.Keywords: amalgam, mercury-resistant bacteria  Abstrak: Logam sangat penting bagi kehidupan manusia. Walaupun demikian beberapa jenis logam memiliki efek toksik, salah satunya ialah merkuri. Merkuri tergolong logam berat dengan tingkat toksisitas yang tinggi. Dalam bidang kedokteran gigi, merkuri digunakan sebagai salah satu bahan campuran amalgam. Penggunaan amalgam ternyata memicu bakteri resisten merkuri dikarenakan pelepasan Hg secara terus menerus sejak dilakukan kondensasi amalgam di dalam mulut. Penelitian ini bertujuan untuk mengetahui apakah terdapat bakteri resisten merkuri dalam urin pasien yang menggunakan amalgam di Puskesmas Tuminting dan untuk mengidentifikasi jenis bakteri resisten merkuri yang ditemukan. Jenis penelitian ialah deskriptif eksploratif. Sampel penelitian ialah urin pasien di Poli Gigi dengan tumpatan amalgam minimal 6 bulan. Hasil penelitian menapatkan dari 30 isolat bakteri resisten merkuri terdapat 6 genus bakteri yang resisten terhadap merkuri, yaitu: Klebsiella sp, Bacillus sp, Staphylococcus sp, Hafnia sp, Enterobacter sp, dan Eubacteria sp. Simpulan: Terdapat bakteri resisten merkuri dalam urin pasien dengan tumpatan amalgam merkuri di Poli Gigi Puskesmas Tuminting.Kata kunci: amalgam, bakteri resisten merkuri

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