scholarly journals Research on Protein Level in Medical Latex Glove Images using Color Kernel Regression Method

2019 ◽  
Vol 8 (6S) ◽  
pp. 612-616
Keyword(s):  
Protein Level ◽  
Healthcare Workers ◽  
Kernel Regression ◽  
Protein Detection ◽  
Color Difference ◽  
Coefficient Of Determination ◽  
Blue Color ◽  
Chemical Test ◽  
Latex Glove ◽  
Latex Gloves

In the healthcare environment, medical latex gloves are a necessary medical item for healthcare workers as it offers excellent hand barrier protection against dangerous microorganism. However, if the healthcare workers repeated exposure to the latex gloves which contain high protein level, it will increase the possibility of the workers to have a risk for latex allergy. Thus, the objective of this project is to develop a color kernel regression (CKR) method for estimating protein level through the analyses of color difference in glove images. Initially, the gloves will go through an uncomplicated chemical test for protein detection. A blue color will appear on the surface of a glove sample that contains protein. After that, the chemical binded sample will be digitally converted into a sample image using the flatbed scanner. The image will then undergo image processing to improve its quality and to calculate the color difference values of the sample. Those calculated values with the pre-defined protein levels will be used to plot a standard graph. A high coefficient of determination with R2 > 98% has been obtained from the experimental graph. This indicates that the proposed CKR method contributes significantly toward the estimation of protein level

scholarly journals Protein Concentration Determination in Latex Glove Using Biocompatibility Morphological Mean Test

2020 ◽  
Vol 2 ◽  
pp. 18-24
Author(s):  
Toa C. K. ◽  
Sim K. S. ◽  
Chan Y. K.
Keyword(s):  
Protein Concentration ◽  
Color Image ◽  
Protein Detection ◽  
Color Difference ◽  
Worst Case ◽  
Latex Glove ◽  
Standard Curve ◽  
Latex Gloves ◽  
Threatening Condition ◽  
Life Threatening

Latex gloves are seen as an indispensable item in the healthcare field because it offers superior protection for both the medical staff and patient against harmful substances. However, latex gloves with high protein concentration have a high possibility to induce latex allergy which in the worst case can lead to a life-threatening condition. To minimize the occurrence of an allergy reaction, the computerized Biocompatibility Morphological Mean (BMM) test for protein detection is proposed. This test initially goes through the chemical process to determine the protein that resides in the glove sample. After that, the sample is electronically converted into a digital image. Finally, the image undergoes color image processing for calculating the color difference values. These values are then plotted on a standard curve. A high correlation coefficient (R2>0.97) of the standard curve gives better accuracies. The proposed method only takes about 40 minutes to complete the test, while existing methods need at least 6 hours.

1376 Latex Allergies: Seeking an Alternative to Minimise Risk in The Operating Theatre

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
A Zargaran ◽  
D Zargaran ◽  
M Ashraf ◽  
P Khanal ◽  
A Srivastava ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Natural Rubber Latex ◽  
Cost Savings ◽  
Allergic Reactions ◽  
Latex Glove ◽  
Latex Gloves ◽  
Additional Costs ◽  
Lost Work Time ◽  
The Uk ◽  
Mean Cost

Abstract Introduction Natural Rubber Latex (NRL) gloves have been ubiquitously used in surgical practice. Over the past few decades, there have been many reports of NRL allergies associated with their use presenting a safety hazard to both patients and healthcare workers. Powder-free and latex-free gloves have been shown to offer reductions in incidence of allergic reactions, as well as cost savings. Method We performed a literature search to identify the rates of reaction to latex across the population in addition to the associated costs including litigation costs, costs of admission, and lost work time associated. We further looked at the rates of perforation of alternatives and the costs associated with such a transition. Results The UK prevalence of sensitisation amongst healthcare workers has been estimated to be 10%. The average cost of litigation to the employer was found to be $21,402 per claim. The mean cost of inpatient care for anaphylaxis has been cited as £469.88, with additional costs such as follow-up representing approximately £400. A 14-month study found cost savings of $10,000pa with a switch to non-latex gloves. Furthermore, an overall perforation rate of 80% in a latex-free glove compared to 34.4% in a latex glove. Conclusions From a business perspective, there is evidence of cost-savings arising from a switch to latex-free gloves, however, formal cost-effectiveness analyses would inform future decisions. Furthermore, comfort and reducing the chances of allergic reactions through removing the offending allergen all represent benefits of switching to latex-free gloves, however, non-inferiority analyses will inform future strategies both locally and nationally.

scholarly journals Latex glove use among healthcare workers in Australia

2018 ◽  
Vol 46 (9) ◽  
pp. 1014-1018 ◽  
Author(s):  
Renee N. Carey ◽  
Lin Fritschi ◽  
Timothy R. Driscoll ◽  
Michael J. Abramson ◽  
Deborah C. Glass ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Latex Glove

scholarly journals Potential Biomarkers of Colorectal Adenoma–Dysplasia–Carcinoma Progression: mRNA Expression Profiling and In Situ Protein Detection on TMAs Reveal 15 Sequentially Upregulated and 2 Downregulated Genes

2009 ◽  
Vol 31 (1) ◽  
pp. 19-29
Author(s):  
Orsolya Galamb ◽  
Ferenc Sipos ◽  
Sándor Spisák ◽  
Barnabás Galamb ◽  
Tibor Krenács ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Progression ◽  
Colorectal Adenoma ◽  
Protein Level ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Protein Detection ◽  
Yield Potential ◽  
Prostaglandin D2 ◽  
Potential Biomarkers

Background: As most colorectal cancers (CRC) develop from villous adenomas, studying alterations in gene expression profiles across the colorectal adenoma–dysplasia–carcinoma sequence may yield potential biomarkers of disease progression.Methods: Total RNA was extracted, amplified, and biotinylated from colonic biopsies of 15 patients with CRC, 15 with villous adenoma and 8 normal controls. Gene expression profiles were evaluated using HGU133Plus2.0 microarrays and disease progression associated data were validated with RT-PCR. The potential biomarkers were also tested at the protein level using tissue microarray samples of 103 independent and 16 overlapping patients.Results: 17 genes were validated to show sequentially altered expression at mRNA level through the normal–adenoma–dysplasia–carcinoma progression. Prostaglandin-D2 receptor (PTGDR) and amnionless homolog (AMN) genes revealed gradually decreasing expression while the rest of 15 genes including osteonectin, osteopontin, collagen IV–alpha 1, biglycan, matrix GLAprotein, and von Willebrand factor demonstrated progressively increasing expression. Similar trends of expression were confirmed at protein level for PTGDR, AMN, osteopontin and osteonectin.Conclusion: Downregulated AMN and PTGDR and upregulated osteopontin and osteonectin were found as potential biomarkers of colorectal carcinogenesis and disease progression to be utilized for prospective biopsy screening both at mRNA and protein levels. Gene alterations identified here may also add to our understanding of CRC progression.

scholarly journals Impact of Replacing Powdered Gloves With Powder-free Gloves on Hand-hygiene Compliance Among Healthcare Workers of an Intensive Care Unit: a Quasi-experimental Study

2020 ◽  
Author(s):  
Mayra Gonçalves Menegueti ◽  
Fernando Bellissimo-Rodrigues ◽  
Marcia A. Ciol ◽  
Maria Auxiliadora-Martins ◽  
Anibal Basile-Filho ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Experimental Study ◽  
Hand Hygiene ◽  
Healthcare Workers ◽  
Hand Hygiene Compliance ◽  
Latex Gloves ◽  
Before And After ◽  
Quasi Experimental ◽  
Hygiene Compliance ◽  
Powdered Latex Gloves

Abstract Background/Objective. After wearing powdered gloves, healthcare workers (HCW) are supposed to wash their hands instead of using alcohol-based hand-rub (ABHR). Washing hands takes longer than using ABHR, and the use of powdered gloves may be an obstacle to hand-hygiene compliance. This study aimed to evaluate the impact of replacing powdered gloves with powder-free gloves on hand-hygiene compliance among HCW of an intensive care unit (ICU). Methods. A quasi-experimental study was conducted in a general ICU of a tertiary care university hospital in Brazil. From June 1st to July 15th, 2017, all HCW were provided with powdered latex gloves only for all clinical procedures. From July 15th to August 31st, 2017, HCW were provided with nitrile powder-free gloves only. Hand-hygiene compliance was assessed through direct observation, and evaluated according to the World Health Organization (WHO) Hand Hygiene guidelines. We calculated that a sample size of 544 hand hygiene opportunities needed to be observed per period. Data analysis were performed using the STATA SE® version 14, and we compared the individual’s percentage of compliance using the t test for paired data before and after the intervention.Results. Overall, 40 HCW were assessed before and after the introduction of nitrile powder-free gloves, with 1114 and 1139 observations of hand hygiene opportunities, respectively. The proportion of compliance with hand hygiene was 55% (95% Confidence Interval [CI]: 51-59%) using powdered latex gloves and 60% (95% CI: 57-63%) using powder-free gloves. The difference in proportions between the two types of gloves was 5.1% (95% CI: 2.5-7.6%, p<0.001).Conclusion. Our data indicate that replacing powdered gloves with powder-free gloves positively influenced hand-hygiene compliance by HCW in an ICU setting.

Fluorescent-Based Temperature Measurement with Simple Compensation of Photo-Degradation Using Hydrogel-Tool and Color Space Conversion

2013 ◽  
Vol 25 (4) ◽  
pp. 596-602 ◽  
Author(s):  
Hisataka Maruyama ◽  
◽  
Taisuke Masuda ◽  
Fumihito Arai
Keyword(s):  
Color Space ◽  
Color Difference ◽  
Temperature Measurements ◽  
Blue Color ◽  
Photo Degradation ◽  
Color Space Conversion ◽  
System A ◽  
Red Color ◽  
Brightness Fluctuation ◽  
Increasing Temperature

We developed a method to obtain stable and longlifetime temperature measurements using a fluorescence micromeasurement system. A hydrogel tool containing nano-semiconductor quantum dots (Q-dots) was developed as a fluorescent temperature indicator. We used image processing to convert RGB information to other color information to compensate for photodegradation. The temperature was calibrated using the hydrogel tool in several color spaces, includingRGB(R: red,G: green,B: blue),HSV(H: hue,S: saturation,V: value (brightness)), andYCrCb(Y: brightness,Cr: red color difference,Cb: blue color difference). The calibration results showed thatR,G,B,Y, andCrdecreased monotonically with increasing temperature, whereasHandCbdid not decrease monotonically. The photodegradation analysis showed thatCrwas robust against the brightness fluctuation; however,R,G, andBstrongly affected the brightness fluctuation because these values included the brightness information. These results show that temperature measurements based onCrvalues are suitable to compensate for photodegradation and have a sensitivity of -1.3%/K and an accuracy of 0.3 K. These values are the same as those obtained using the fluorescence intensity method.

Anti-Microbial and Self-Cleaning of Natural Rubber Latex Gloves by Adding Mangosteen Peel Powder

2018 ◽  
Vol 777 ◽  
pp. 3-7 ◽  
Author(s):  
Wasan Moopayuk ◽  
Nuchnapa Tangboriboon
Keyword(s):  
Tensile Strength ◽  
Natural Rubber ◽  
Natural Rubber Latex ◽  
Film Surface ◽  
Latex Film ◽  
Rubber Latex ◽  
Elongation At Break ◽  
Latex Glove ◽  
Latex Gloves ◽  
Mangosteen Peel

Mangosteen peel powder is one of the most important bio-antioxidants. Adding mangosteen peel powder as filler into natural rubber latex compound for latex glove film formation via dipping process can help the green anti-microbial properties. The physical (smoothness and thickness of film) and mechanical properties (tensile strength and elongation at break) of latex film are still good. Therefore, adding mangosteen peel powder into natural rubber latex gloves can reduce the anti-allergic and antimicrobial on the film surface. Mangosteen peel powder ground by rapid mill is fine particle and high surface area 2.4216 m2/g suitable for homogeneous and compatible for adding into natural rubber latex compound. Ceramic hand mold was dipped into the Ca (NO3)2 coagulant only 3 seconds, then dipped into the natural rubber latex compounds added mangosteen peel powder for 15 seconds, withdrawn hand mold slowly, cured in the oven at 120°C for 30 min, then dried at room temperature, and casted it off the hand mold. The obtained natural latex glove films added mangosteen peel powder are smooth, clear, and thin film surface, the highest elongation at break 803.2711 ± 31.6477%, good tensile strength 30.2933 ± 6.0218 MPa, dense film without water leakage, and good contact angle.

scholarly journals Persistence of Candida auris on latex and nitrile gloves with transmission to sterile urinary catheters‡

2019 ◽  
Vol 58 (1) ◽  
pp. 128-132
Author(s):  
Kauser Jabeen ◽  
Pushpa Bhawan Mal ◽  
Areeba Tharwani ◽  
Mariam Hashmi ◽  
Joveria Farooqi
Keyword(s):  
Urinary Catheter ◽  
Urinary Catheters ◽  
Candida Auris ◽  
Candida Spp ◽  
Time Intervals ◽  
Plate Method ◽  
Latex Glove ◽  
Latex Gloves ◽  
Roll Plate ◽  
Agar Surface

Abstract Candida auris’ ability to persist on contaminated gloves and transmit to urinary catheters was evaluated. 105 and 103 cfu/ml suspensions of eight Candida species including C. auris were inoculated on latex and nitrile gloves fingertips and touched on agar surface at different time intervals. Urinary catheter piece, touched by latex glove carrying Candida spp. suspensions at various time intervals, was cultured by roll-plate method. C.auris persisted on latex gloves at both 105 and 103 cfu/ml up to 3 minutes and could be transmitted from both wet and dry contaminated gloves to catheters. Proper glove use with strict hand hygiene should be advocated in settings with ongoing C.auris transmission.

scholarly journals Impact of replacing powdered gloves with powder-free gloves on hand-hygiene compliance among healthcare workers of an intensive care unit: a quasi-experimental study

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Mayra Gonçalves Menegueti ◽  
Fernando Bellissimo-Rodrigues ◽  
Marcia A. Ciol ◽  
Maria Auxiliadora-Martins ◽  
Anibal Basile-Filho ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Experimental Study ◽  
Hand Hygiene ◽  
Healthcare Workers ◽  
Hand Hygiene Compliance ◽  
Latex Gloves ◽  
Before And After ◽  
Quasi Experimental ◽  
Hygiene Compliance ◽  
Powdered Latex Gloves

Abstract Background/objective After wearing powdered gloves, healthcare workers (HCW) are supposed to wash their hands instead of using alcohol-based hand-rub (ABHR). Washing hands takes longer than using ABHR, and the use of powdered gloves may be an obstacle to hand-hygiene compliance. This study aimed to evaluate the impact of replacing powdered gloves with powder-free gloves on hand-hygiene compliance among HCW of an intensive care unit (ICU). Methods A quasi-experimental study was conducted in a general ICU of a tertiary care university hospital in Brazil. From June 1st to July 15th, 2017, all HCW were provided with powdered latex gloves only for all clinical procedures. From July 15th to August 31st, 2017, HCW were provided with nitrile powder-free gloves only. Hand-hygiene compliance was assessed through direct observation, and evaluated according to the World Health Organization Hand Hygiene guidelines. We calculated that a sample size of 544 hand hygiene opportunities needed to be observed per period. Data analysis were performed using the STATA SE® version 14, and we compared the individual’s percentage of compliance using the t test for paired data before and after the intervention. Results Overall, 40 HCW were assessed before and after the introduction of nitrile powder-free gloves, with 1114 and 1139 observations of hand hygiene opportunities, respectively. The proportion of compliance with hand hygiene was 55% (95% confidence interval [CI] 51–59%) using powdered latex gloves and 60% (95% CI 57–63%) using powder-free gloves. The difference in proportions between the two types of gloves was 5.1% (95% CI 2.5–7.6%, p < 0.001). Conclusion Our data indicate that replacing powdered gloves with powder-free gloves positively influenced hand-hygiene compliance by HCW in an ICU setting.

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