scholarly journals Ação da atividade antifúngica in vitro dos óleos essenciais de Copaifera officinalis, Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis e Thymus vulgaris ante os agentes causais de onicomicose

2019 ◽  
Vol 1 (2) ◽  
pp. 56-64
Author(s):  
Gyzelle Pereira Vilhena do Nascimento ◽  
Juliane Viegas de Oliveira
Keyword(s):  
Candida Albicans ◽  
Trichophyton Rubrum ◽  
Rosmarinus Officinalis ◽  
Thymus Vulgaris ◽  
Microsporum Canis ◽  
Melaleuca Alternifolia

A onicomicose é definida como uma doença fúngica caracterizada pelo processo infeccioso da região é ungueal e, é responsável por desencadear uma má qualidade de vida aos pacientes e apesar de serem consideradas micoses superficiais, são difíceis de diagnosticar e tratar, com isso, tem impulsionado a realização de pesquisas na tentativa de obter outras opções terapêuticas além dos medicamentos sintéticos disponíveis na área da saúde. Diante do exposto, o presente estudo teve por finalidade avaliar a ação in vitro dos óleos essenciais de Copaifera officinalis, Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis e Thymus vulgaris frente a fungos causadores de onicomicose. Para a pesquisa da atividade antifúngica in vitro dos óleos essenciais selecionados foi utilizado o método teste de placa de ágar com orifício frente às cepas de Candida albicans (ATCC 10231), Microsporum canis (ATCC 14055) e Trichophyton rubrum (ATCC 28189), nas proporções de 100%, 25% e 5% sendo a leitura das placas e interpretação dos resultados avaliados a partir da observação do halo de inibição, considerando a área sem crescimento detectável a olho nu. Observou-se que os óleos essenciais de Eugenia caryophylata, Melaleuca alternifolia, Rosmarinus officinalis e Thymus vulgaris apresentaram atividade antifúngica com formação de halos sobre as espécies fúngicas leveduras e dermatófitos, sendo que o óleo de Copaifera officinalis, apresentou apenas uma fraca atividade em relação as espécies fúngicas testadas. Portanto, conclui-se que os óleos essenciais analisados apresentaram atividade antifúngica, abrindo a perspectiva de novos estudos para caracterização de seus princípios antimicrobianos e alternativas ditas naturais para tratamento dessa podopatia.

scholarly journals In vitro evaluation of the antifungal activity of Marco (Ambrosia arborescens Mill.) and Matico (Aristeguietia glutinosa Lam.) on the pathogenic fungi that cause dermatomycosis (ringworm)

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 559
Author(s):  
Tatiana de los Ángeles Mosquera Tayupanta ◽  
Sandra Elizabeth Ayala Valarezo ◽  
Tatiana Alexandra Vasquez Villareal ◽  
María Belén Montaluisa Álvarez
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Therapeutic Potential ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
The One ◽  
One Way Anova

Background: Currently, there is a trend towards using natural and ethnopharmacological species with therapeutic potential. This investigation evaluated the antifungal activity of two species in the Ecuadorian Andes, which are used in treating dermatomycosis: Ambrosia arborescens Mill. (Marco) and Aristeguietia glutinosa Lam. (Matico). Methods: We worked with seven concentrations (100 to 700ppm) of Ambrosia arborescens Mill. extract and ten concentrations (0.5 to 5%) of essential oil (EO) of Aristeguietia glutinosa Lam. on Trichophyton mentagrophytes ATCC 9533, Trichophyton rubrum ATCC 28188, Microsporum canis ATCC 36299 and Candida albicans ATCC 10231. The methodology used was a modified version of the Kirby-Bauer method, using diffusion in agar wells. Results: The Tukey test, after the one-way Anova, determined effective concentrations of EO: 5% for Trichophyton mentagrophytes, 4.5% for Trichophyton rubrum, 5% for Microsporum canis and 2% for Candida albicans. In the extracts, the concentration of 700ppm was used for Trichophyton mentagrophytes, Trichophyton rubrum, and 600ppm for Microsporum canis and Candida albicans. Conclusions: The evaluation of the antifungal activity of the Ambrosia arborescens extract showed inhibition in the studied dermatophytes in each one of the planted concentrations (100 to 700ppm). The evaluation of the antifungal activity of Aristeguietia glutinosa EO showed inhibition in the studied dermatophytes in each of the planted concentrations (0.5 to 5%).

scholarly journals ANTI-FUNGAL ACTIVITY OF ACALYPHA WILKESIANA: A PRELIMINARY STUDY OF FUNGAL ISOLATES OF CLINICAL SIGNIFICANCE

2021 ◽  
Vol 16 (1) ◽  
pp. 21-30
Author(s):  
Katibi Oludolapo Sherifat ◽  
Aboh Mercy Itohan ◽  
Salawu Oluwakayinsola Adeola ◽  
Kola -Mustapha Adeola ◽  
Olatunji Lawrence Aderemi
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Seborrheic Dermatitis ◽  
Ethanol Extract ◽  
Microsporum Canis ◽  
Plant Materials ◽  
Acalypha Wilkesiana ◽  
Anti Fungal

Acalypha wilkesiana (AW), a popular medicinal plant has been used in traditional medicine to treat a variety of skin disorders including pityriasis versicolor and seborrheic dermatitis. As a prelude to clinical trials in humans, an experimental study was carried out to determine the spectrum of antifungal activity of 2 variants of the Acalypha wilkesiana plant. Materials and Methods: The ethanol extract and herbal cream formulation of the dried leaves of 2 cultivars (Macrophylla & Hoffmani) of Acalypha wilkesiana were investigated for in-vitro antifungal activity by disc diffusion and micro-broth dilution techniques. Organisms tested were typed cultures of Malassezia furfur, Candida albicans and Trichophyton rubrum; and clinical strains of Microsporum canis and Epidermophyton floccosum. Results: Both cultivars (Macrophylla and Hoffmanii) of the plant showed good activity against all the fungi tested except Microsporum canis (8.0±0.00; 7.00±0.00 mm). The greatest activity was observed against Trichophyton rubrum (22.0±0.00; 24.00±0.00 mm) while Candida albicans showed the least activity (15.0±0.00; 18.00±0.57 mm). The Minimum Inhibitory Concentration (MIC) of the crude extract ranged between 0.25 and 8 mg/ml for all organisms, while that of the herbal cream was 0.31-8mg/ml. The lowest MIC was seen with Candida albicans for both varieties of the plant. The Acalypha wilkesiana Hoffmanii demonstrated a greater activity against Candida albicans and Malassezia furufur than the A. wilkesiana (Macrophylla). Conclusion: This study reveals Acalypha wilkesiana leaf extract has potential for development as a cream that can be used to treat superficial fungal skin infections.

scholarly journals Essential oils in ocular pathology: an experimental study

2015 ◽  
Vol 9 (06) ◽  
pp. 650-654 ◽  
Author(s):  
Sara Cannas ◽  
Donatella Usai ◽  
Antonio Pinna ◽  
Stefania Benvenuti ◽  
Roberta Tardugno ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Bacterial Infections ◽  
In Vitro Cytotoxicity ◽  
Rosmarinus Officinalis ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Melaleuca Alternifolia ◽  
Natural Drugs

Introduction: The antimicrobial activity of essential oils (EOs) has been known for ages; in particular, the EOs of Melaleuca alternifolia, Thymus vulgaris, Mentha piperita, and Rosmarinus officinalis have been used for the treatment of fungal and bacterial infections. Methodology: This study focused on the in vitro cytotoxicity to normal human conjunctiva cells and antimicrobial activity of 20 EOs. Results: The oils tested showed no cytotoxic effect at very low concentrations. Rosmarinus officinalis, Melaleuca alternifolia, and Thymus vulgaris L. red thyme geraniol sel oils had good antimicrobial activity against Gram-positive and Gram-negative strains. Conclusions: The results of this study are of great interest and may have a major impact on public health, providing useful tips to optimize the therapeutic use of some natural drugs.

scholarly journals The efficacy of certain Labiatiae species herbal extracts (Rosmarinus officinalis, Salvia officinalis and Thymus vulgaris) as compared to nystatin, in the inhibition of in vitro growth of Candidia albicans

2002 ◽  
Author(s):  
◽  
Kim Louise Reid
Keyword(s):  
Candida Albicans ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Vulgaris ◽  
In Vitro Growth ◽  
Disc Diffusion ◽  
Herbal Extracts ◽  
Diffusion Test

The purpose of this study was to determine the effect of herbal extracts of certain Labiatiae species (Rosmannus officinalis, Salvia officinalis and Thymus vulgaris) in the inhibition of in vitro growth of Candida albicans as compared to ethanol as a control and nystatin in terms of the disc diffusion test.

scholarly journals In Vitro Efficacy of Essential Oils from Melaleuca Alternifolia and Rosmarinus Officinalis, Manuka Honey-based Gel, and Propolis as Antibacterial Agents Against Canine Staphylococcus Pseudintermedius Strains

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 344
Author(s):  
Gabriele Meroni ◽  
Elena Cardin ◽  
Charlotte Rendina ◽  
Valentina Rafaela Herrera Millar ◽  
Joel Fernando Soares Filipe ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Rosmarinus Officinalis ◽  
Diffusion Method ◽  
Melaleuca Alternifolia ◽  
Disk Diffusion Method ◽  
Staphylococcus Pseudintermedius ◽  
Tea Tree

Essential oils (EOs) and honeybee products (e.g., honey and propolis) are natural mixtures of different volatile compounds that are frequently used in traditional medicine and for pathogen eradication. The aim of this study was to evaluate the antibacterial properties of tea tree (Melaleuca alternifolia) EO (TTEO), Rosmarinus officinalis EO (ROEO), manuka-based gel, and propolis against 23 strains of Staphylococcus pseudintermedius (SP) isolated from canine pyoderma. Antimicrobial resistance screening was assessed using a panel of nine antimicrobial agents coupled with a PCR approach. An aromatogram was done for both EOs, using the disk diffusion method. The minimum inhibitory concentration (MIC) was determined for all the compounds. Among the 23 SP strains, 14 (60.9%) were multidrug-resistant (MDR), 11 strains (47.8%) were methicillin-resistant (MRSP), and 9 (39.1%) were non-MDR. The mean diameter of the inhibition zone for Melaleuca and Rosmarinus were 24.5 ± 8.8 mm and 15.2 ± 8.9 mm, respectively, resulting as statistically different (p = 0.0006). MIC values of TTEO and ROEO were similar (7.6 ± 3.2% and 8.9 ± 2.1%, respectively) and no statistical significances were found. Honeybee products showed lower MIC compared to those of EOs, 0.22 ± 0.1% for Manuka and 0.8 ± 0.5% for propolis. These findings reveal a significant antibacterial effect for all the tested products.

scholarly journals An evaluation of the antifungal activity of some local medicinal plants against growth of Candida albicans in vitro

2010 ◽  
Vol 9 (2) ◽  
pp. 60
Author(s):  
J.S. Al-Hussaini, and A. M. G. Al-Mohana
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Culture Media ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Elettaria Cardamomum ◽  
Local Plants

The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum, Aloe vera, Thyme Vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in the present study (25, 50, 100, 150, 200, and 400) mg/ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans

scholarly journals ANTIFUNGAL ACTIVITIES OF ETHANOL EXTRACT OF ROSEMARY LEAF (Rosemarinus officinalis L.) AGAINST Candida albicans

2020 ◽  
Vol 2 (1) ◽  
pp. 28
Author(s):  
Dewa Ayu Made Adnya Swari ◽  
I Wayan Martadi Santika ◽  
I Gusti Made Aman
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Rosmarinic Acid ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Control Group ◽  
Rosmarinus Officinalis L

Background: This study was a preliminary study aimed to investigating the in vitro activity of rosmarinic acid contained on Rosemary leaves (Rosemarinus officinalis L.) on the Candida albicans. Rosmarinic acid can be an inhibitor of the RTPase enzyme in the fungus Candida albicans. Objective: This study is expected to provide information in the development of science about the antifungal activity of ethanol extract of Rosemary leave (Rosmarinus officinalis L.) againsts C.albicans. The recent research was an laboratory experimental. Experiments on an ethanol extract of Rosemary leaves (Rosemarinus officinalis L.) and post-test design of the control group. Methods: The extraction method used in this study was a multilevel extraction method. The antifungal activity testing method uses discs and MIC methods. Replication was done six times at two concentration namely, 80% (b/v) and 100% for each measurement. Results: The data analysis was carried out using SPSS with 95% of confidence level with the Kruskal Wallis analysis then completed with the Mann Whitney U test. The results of the MIC experiment showed all the various concentrations of ethanol extract performed Candida albicans growth on the surface of the media. Extract with 80% (b/v) concentration showed greater antifungal activity. Furthermore the MIC was shown by 80% (b/v) concentration of the ethanol extract of Rosemary leaves against Candida albicans. The test results of the antifungal activity of the Rosemary leaf ethanol extract showed the presence of inhibitory diameter in the ethanol extract of the Rosemary leaf concentration of 80% (b/v) with a mean ± SD of 10.6 ± 0.57735 and RSD of 5.774%. Conclusion: The ethanol extract of Rosemary leaves (Rosmarinus officinalis L.) has an inhibitory effect on the fungus Candida albicans at a concentration of 80% (b / v) Keywords: Antifungal activity, Candida albicans, Rosemary, RTPase, ethanol extract

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