EFEKTIVITAS KOMBINASI PLASMA JET NON -THERMAL DAN SPRAY Aloe vera (L). Burm. f . PADA PENYEMBUHAN LUKA DIABETES

Plasma jet non thermal has benefit in wound healing, but less control can to accumulation of reactive oxygen species. This study aims to determine the effectiveness of the combination of plasma jet and A. vera spray 1% for wound healing in diabetic wounds mice model with parameters of malondialdehyde levels, the skin wound ratio, and blood profile. This study used male Balb/C were induced by a streptozotocin (STZ) and divided into 4 groups, namely groups of untreated diabetic mice wounds (N), groups of diabetic mice wound with A. vera spray 1% (Av), groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes (P), and groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes and A. vera spray 1% (PAv). The solution of A. vera spray 1% preparation consists of A. vera, DMSO, and aquabidest extracts. The wounds of all groups treated for 14 days then the malondialdehyde, skin wound ratio and blood profile were calculated by SPSS Statistic. The results of blood glucose before treatment were 241.74 dL ± 51.99. The combination of plasma jet and A. vera spray 1% groups were significant (p <0.05) in reduce malondialdehyde levels, reduced the the skin wound ratio but not different significantly (p> 0.05), and not effect on blood profile data (p> 0.05). In conclusion, A. vera spray 1% can reduce the side effects of plasma jet in diabetic wounds by reducing malondialdehyde levels.

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Efektivitas Perlakuan Kombinatif Plasma Medis dan Ekstrak Daun Sirih untuk Mempercepat Penyembuhan Luka Fase Proliferasi pada Model Mencit Diabetik

Jurnal Ilmiah Kesehatan Keperawatan ◽

10.26753/jikk.v15i2.378 ◽

2019 ◽

Vol 15 (2) ◽

pp. 81

Author(s):

Eka Sakti Wahyuningtyas ◽

Nasruddin Nasruddin ◽

Heni Setyowati Esti Rahayu ◽

Heni Lutfiyati ◽

Isabella Meliawati Sikumbang ◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Plasma Jet ◽

Diabetic Wound ◽

Diabetic Mice ◽

Plasma Medicine ◽

Betel Leaf ◽

Diabetic Wound Healing ◽

Diabetic Wounds ◽

Piper Betel Leaf

The continued increase in the number of people with Diabetes Mellitus (DM) in Indonesia is a serious problem. One of the big problems for people with Diabetes Mellitus (DM) is the emergence of complications of diabetic wounds. To date the strategy for treatment of diabetic wounds has been limited to the use of wound dressing, cell therapy and oxygen therapy. The problem is that the strategy is not fully successful. Thus, it is very important to look for new strategies to improve the quality of diabetic wound healing, such as by applying a combination of plasma medicine and local natural product, like the extraction of Daun sirih (Piper betle) leaves. Plasma medicine is a relatively new and multidisciplinary study involving plasma science, biomedical, pharmaceutical and other health sciences aimed at applying plasma to therapeutic health. Plasma is the fourth phase of matter, after the solid, liquid and gas phase. The medical aspects of plasma are related to the ability of plasma to produce biological molecules Reactive Oxygen and Nitrogen Species (RONS). If RONS is controlled in the right dosage it can be efficacious for health therapy. This study intends to examine the effects of combinative treatment of plasma medicine and Piper betel leaf extract for proliferation phase of wound healing in diabetic small animal model. This study used male Balb c mice with acute wounds which were divided into 5 groups, namely groups of untreated normal mice (ND-TP), groups of untreated diabetic mice (D-TP), groups of diabetic mice wounds with Piper betel leaf extract (DS ), the wound group of diabetic mice with plasma medicine (DP) and the wound group of diabetic mice with plasma medicine and Piper betel leaf (DPS). The plasma medicine was treated on wound with condition non-contact style (the plasma jet did not touch the wound) with a distance of plasma jet reactor nozzle to the surface of wound about 20 mm, for 2 minutes, every day. Macroscopic observation of wounds is carried out every day from day 0 to 7. On day 7 it was seen that the size of the wound area for D-P-S was smaller than the other groups. The results of this study indicated that Piper betel leaf extract can potentially be used to optimize the performance of plasma medicine in accelerating diabetic wound healing during the proliferation phase. Further investigation, however, is important to be conducted to study the effect for all phases of wound healing and its mechanism histo-pathologically.

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Neuroprotectin/protectin D1: endogenous biosynthesis and actions on diabetic macrophages in promoting wound healing and innervation impaired by diabetes

AJP Cell Physiology ◽

10.1152/ajpcell.00270.2014 ◽

2014 ◽

Vol 307 (11) ◽

pp. C1058-C1067 ◽

Cited By ~ 28

Author(s):

Song Hong ◽

Haibin Tian ◽

Yan Lu ◽

James Monroe Laborde ◽

Filipe A. Muhale ◽

...

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Chronic Inflammation ◽

Time Course ◽

Skin Wound ◽

Diabetic Wound ◽

Diabetic Mice ◽

Diabetic Wound Healing ◽

Diabetic Wounds ◽

And Oxidative Stress

Dysfunction of macrophages (MΦs) in diabetic wounds impairs the healing. MΦs produce anti-inflammatory and pro-resolving neuroprotectin/protectin D1 (NPD1/PD1, 10 R,17 S-dihydroxy-docosa-4 Z,7 Z,11 E,13 E,15 Z,19 Z-hexaenoic acid); however, little is known about endogenous NPD1 biosynthesis by MΦs and the actions of NPD1 on diabetic MΦ functions in diabetic wound healing. We used an excisional skin wound model of diabetic mice, MΦ depletion, MΦs isolated from diabetic mice, and mass spectrometry-based targeted lipidomics to study the time course progression of NPD1 levels in wounds, the roles of MΦs in NPD1 biosynthesis, and NPD1 action on diabetic MΦ inflammatory activities. We also investigated the healing, innervation, chronic inflammation, and oxidative stress in diabetic wounds treated with NPD1 or NPD1-modulated MΦs from diabetic mice. Injury induced endogenous NPD1 biosynthesis in wounds, but diabetes impeded NPD1 formation. NPD1 was mainly produced by MΦs. NPD1 enhanced wound healing and innervation in diabetic mice and promoted MΦs functions that accelerated these processes. The underlying mechanisms for these actions of NPD1 or NPD1-modulated MΦs involved 1) attenuating MΦ inflammatory activities and chronic inflammation and oxidative stress after acute inflammation in diabetic wound, and 2) increasing MΦ production of IL10 and hepatocyte growth factor. Taken together, NPD1 appears to be a MΦs-produced factor that accelerates diabetic wound healing and promotes MΦ pro-healing functions in diabetic wounds. Decreased NPD1 production in diabetic wound is associated with impaired healing. This study identifies a new molecular target that might be useful in development of more effective therapeutics based on NPD1 and syngeneic diabetic MΦs for treatment of diabetic wounds.

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Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽

10.3390/molecules26092554 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2554

Author(s):

Marek Konop ◽

Anna K. Laskowska ◽

Mateusz Rybka ◽

Ewa Kłodzińska ◽

Dorota Sulejczak ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Healing Process ◽

Skin Wound ◽

Wound Healing Process ◽

Diabetic Mice ◽

Full Thickness ◽

Skin Wound Healing ◽

Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

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Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽

10.7717/peerj.10232 ◽

2021 ◽

Vol 9 ◽

pp. e10232

Author(s):

Muniba Tariq ◽

Hafiz Muhammad Tahir ◽

Samima Asad Butt ◽

Shaukat Ali ◽

Asma Bashir Ahmad ◽

...

Keyword(s):

Wound Healing ◽

Silk Fibroin ◽

Chronic Wounds ◽

Histological Analysis ◽

Healing Process ◽

Aloe Vera ◽

Wound Contraction ◽

Wound Dressings ◽

Control Group ◽

Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

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Promotion of Wound Healing of Genetic Diabetic Mice Treated by a Cold Atmospheric Plasma Jet

IEEE Transactions on Plasma Science ◽

10.1109/tps.2019.2928320 ◽

2019 ◽

Vol 47 (11) ◽

pp. 4848-4860 ◽

Cited By ~ 3

Author(s):

Donghai Li ◽

Guiling Li ◽

Jing Li ◽

Zhi-Qiang Liu ◽

Xuman Zhang ◽

...

Keyword(s):

Wound Healing ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Diabetic Mice ◽

Cold Atmospheric Plasma

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miRNA-221-3p in Endothelial Progenitor Cell-Derived Exosomes Accelerates Skin Wound Healing in Diabetic Mice

Diabetes Metabolic Syndrome and Obesity Targets and Therapy ◽

10.2147/dmso.s243549 ◽

2020 ◽

Vol Volume 13 ◽

pp. 1259-1270 ◽

Cited By ~ 3

Author(s):

Juan Xu ◽

Suwen Bai ◽

Yadi Cao ◽

Lei Liu ◽

Yang Fang ◽

...

Keyword(s):

Wound Healing ◽

Endothelial Progenitor Cell ◽

Progenitor Cell ◽

Skin Wound ◽

Diabetic Mice ◽

Endothelial Progenitor ◽

Skin Wound Healing

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Examination of the Therapeutic Potential of Mouse Oral Mucosa Stem Cells in a Wound-Healing Diabetic Mice Model

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17134854 ◽

2020 ◽

Vol 17 (13) ◽

pp. 4854

Author(s):

Shiri Kuperman ◽

Ram Efraty ◽

Ina Arie ◽

Arkadi Rahmanov ◽

Marina Rahmanov Gavrielov ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Oral Mucosa ◽

Therapeutic Potential ◽

Mice Model ◽

Healing Response ◽

Wound Healing Response ◽

Diabetic Wound Healing ◽

Diabetic Wounds ◽

Enhance Wound Healing

Diabetic wounds’ delayed healing response is still considered a major therapeutic challenge. Stem cells and derived cellular products have been an active field of research for novel therapies referred to as regenerative medicine. It has recently been shown that human oral mucosa stem cells (hOMSCs) are a readily accessible source for obtaining large quantities of stem cells. This study evaluates the potential of mouse oral mucosa stem cells (mOMSCs) to enhance wound healing in a diabetic (db/db) mouse model by morphological and histological analysis. We show that mOMSCs-treated wounds displayed a significantly faster wound-healing response (p ≤ 0.0001), featuring faster re-epithelialization and a larger area of granulation tissue (p ≤ 0.05). Taken together, these results suggest that oral mucosa stem cells might have therapeutic potential in diabetic wound healing.

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Aloe vera: A Medicinal Plant Used in Skin Wound Healing

Tissue Engineering Part B Reviews ◽

10.1089/ten.teb.2020.0236 ◽

2020 ◽

Author(s):

Jiaheng Liang ◽

Longlong Cui ◽

Jiankang Li ◽

Shuaimeng Guan ◽

Kun Zhang ◽

...

Keyword(s):

Wound Healing ◽

Medicinal Plant ◽

Aloe Vera ◽

Skin Wound ◽

Skin Wound Healing

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ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018v10i2.24217 ◽

2018 ◽

Vol 10 (2) ◽

pp. 57 ◽

Cited By ~ 4

Author(s):

Nasrul Wathoni ◽

Aliya Nur Hasanah ◽

Ahmed Fouad Abdelwahab Mohammed ◽

Elasari Dwi Pratiwi ◽

Ripa’atul Mahmudah

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Growth Factor ◽

Keratinocyte Growth Factor ◽

Research Work ◽

Physicochemical Characterization ◽

Swelling Ratio ◽

Diabetic Mice ◽

Mice Model ◽

Hydrogel Film

Objective: The main objective of the research work was to fabricate sacran hydrogel film containing keratinocyte growth factor (Sacran/KGF-HGF), and to evaluate their wound healing ability in alloxan-induced diabetic mice model.Methods: The physicochemical characterization of Sacran/KGF-HGF were investigated by thickness, tensile strength, swelling ratio, x-ray diffractometer (XRD), scanning electron microscope (SEM), and biodegradability. The wound healing ability was investigated by creating two full-thickness excisional wounds inalloxan-induced diabetic mice.Results: The thickness, tensile strength, and swelling ratio results showed that KGF in the Sacran/KGF-HGF improved not only the thickness of sacran hydrogel film (Sacran-HGF), but also the tensile strength and swelling ability of Sacran-HGF. The XRD and SEM results confirmed that the Sacran/KGF-HGF were amorphous and similar morphology to Sacran-HGF, respectively. The biodegradability results revealed that the Sacran/KGF-HGF degraded for about 41.29% in trichloroacetic acid (TCA) and 22.92% in TrypLE™ (recombinant enzyme) solutions. In addition, KGF improved the degradability of Sacran/KGF-HGF in both solutions. Interestingly, the Sacran/KGF-HGF, which was applied on wound site, considerably improved the wound healing ability of Sacran-HGF at 6, 9 and 12 d in alloxan-induced diabetic mice model, compared to control (non-treated).Conclusion: These results suggest that KGF has the potential to promote the chronic wound healing ability of Sacran-HGF.

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