scholarly journals Genetic diversity between native cupuízeiros from Portal da Amazonia, Mato Grosso, Brazil

2020 ◽  
Vol 13 (3) ◽  
pp. 51
Author(s):  
A. G. M. Cordeiro ◽  
L. H. R. Silva ◽  
E. S. Cardoso ◽  
G. F. Pena ◽  
K. E. M. Zortéa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Optimization Method ◽  
Jaccard Coefficient ◽  
Mato Grosso ◽  
Fruit Species ◽  
Wild Fruit ◽  
Inter Simple Sequence Repeats ◽  
Commercial Species ◽  
Ecological Importance

Theobroma subincanum Mart. popularly known as cupuí, belongs to the family Malvaceae. It is a wild fruit species native to the Amazon and is found in mainland forests, being characterized by its great alimentary and ecological importance. The cupuí presents genes that can be used for the croop breeding of commercial species of the genus, so that many studies have been carried to characterize genotypes through molecular markers, among which ISSRs (Inter Simple Sequence Repeats) have been widely used. In this context the objective of the study was to analyze the genetic diversity among cupuí genotypes, using molecular markers ISSR. Ten markers used amplified 42 fragments, being 35.71% polymorphic, and the genetic dissimilarity, estimated from the Jaccard coefficient, varied from 0.02 to 0.26, indicating the existence of genetic variability among the studied genotypes. The UPGMA grouping method allocated the twenty genotypes of cupuí analyzed in three groups, while the Tocher optimization method distributed the same in eight groups, thus confirming the existence of genetic diversity among the evaluated materials. The two methods of grouping revealed that the MN08, MN09 and MN10 individuals are the most divergent and therefore the most suitable for conservation and breeding programs of the species.

scholarly journals Genetic diversity and conservation of native populations of Maytenus Ilicifolia Mart. ex Reiss

2009 ◽  
Vol 69 (2) ◽  
pp. 447-453 ◽  
Author(s):  
AJ. Mossi ◽  
RL. Cansian ◽  
O. Leontiev-Orlov ◽  
JL. Cechet ◽  
AZ. Carvalho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Rio Grande ◽  
Jaccard Coefficient ◽  
Rio Grande Do Sul ◽  
Mato Grosso ◽  
Native Populations ◽  
Maytenus Ilicifolia ◽  
Mato Grosso Do Sul

The aim of this work was to analyze genetic variability in 18 populations of Maytenus ilicifolia, and representatives of Maytenus aquifolia and Maytenus evonymoidis, collected in the states of Mato Grosso do Sul, Paraná, Santa Catarina and Rio Grande do Sul, using RAPD molecular markers. Considering total samples of the three species, 263 amplified fragments were identified, of which 72.2% showed to be polymorphous. The index of similarity (Jaccard coefficient) was on average 0.64 between M. ilicifolia and M. aquifolia; 0.47 between M. ilicifolia and M. evonymoidis; and 0.44 between M. aquifolia and M. evonymoidis. The analysis of groupings by the UPGMA algorithm allowed to clearly separate the three analyzed species. In determining the variability in M. ilicifolia, 222 bands were identified, on average 11.1 bands per primer, being 43.2% polymorphous. The index of similarity (Jaccard coefficient) in the bulks of each population in M. ilicifolia was, on average, 0.92 and the index of similarities among the populations was 0.83. The analysis of groupings with the UPGMA algorithm and the analysis of the main coordination (PCO), allowed the separation of the analyzed populations into three groups, the populations from the south of Rio Grande do Sul and the population from Mato Grosso do Sul standing out. A relation between the groupings found and the edaphoclimatic conditions of the collecting places was observed.

Assessment of Genetic Diversity of Rheum species (Endangered Medicinal Herb of Indian Himalayan Region) using Molecular Markers

2021 ◽  
Vol 16 (11) ◽  
pp. 147-154
Author(s):  
Anjali Uniyal ◽  
Akhilesh Kumar ◽  
Sweta Upadhyay ◽  
Vijay Kumar ◽  
Sanjay Gupta
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Inter Simple Sequence Repeats ◽  
Pharmaceutical Industries ◽  
Endangered Medicinal Herb ◽  
Identification And Characterization ◽  
Simple Sequence

The Rheum species are important medicinal plants that are facing extinction due to their unplanned development and overexploitation by pharmaceutical industries. DNA polymorphisms are not prone to environmental modifications, thus they are widely used for the identification and characterization of plants. The use of different molecular markers has enabled the researchers for the valuation of genetic variability and diversity in its natural zone of distribution. The conventional approach may take several years to yield this information. For the estimation of molecular and genetic variations in geographical zone of distribution, various molecular markers technique are available like RAPD (Randomly Amplified Polymorphic DNA), RFLP (Restriction fragment length polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR and AFLP. The uses of different molecular markers for the study of genetic diversity have been discussed in the review.

scholarly journals ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

2009 ◽  
Vol 37 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Salima Machkour‐M'Rabet ◽  
Yann Hénaut ◽  
Ariane Dor ◽  
Gabriela Pérez-Lachaud ◽  
Céline Pélissier ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Genetic Diversity in <i>Jatropha platyphylla</i> Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers

2021 ◽  
Vol 12 (11) ◽  
pp. 1658-1672
Author(s):  
Edith Salazar-Villa ◽  
Lilia Alcaraz-Meléndez ◽  
Josefina León-Félix ◽  
José Basilio Heredia ◽  
Federico Soto-Landeros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems

2005 ◽  
Vol 62 (4) ◽  
pp. 366-373 ◽  
Author(s):  
Mirian Perez Maluf ◽  
Milene Silvestrini ◽  
Luciana Machado de Campos Ruggiero ◽  
Oliveiro Guerreiro Filho ◽  
Carlos Augusto Colombo
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Inbred Line ◽  
Coffea Arabica ◽  
Ssr Marker ◽  
Cultivar Identification ◽  
Inbred Lines ◽  
Parental Origin ◽  
Commercial Species

One of the greatest problems in Coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. Recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. This work evaluates the reliability of three marker systems: RAPD, AFLP and SSR, to characterize the genetic variability of commercially-used Coffea inbred lines developed by the Instituto Agronômico (IAC), and their potential for cultivar identification. All methods identified polymorphisms among the cultivars. The genetic diversity recognized by the methods is very similar, although is very narrow. RAPD and SSR marker systems grouped more efficiently the evaluated cultivars according to parental origin. None of the methods allowed inbred line identification. Therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.

scholarly journals Genetic variations among passion fruit species using rapd markers

2002 ◽  
Vol 24 (3) ◽  
pp. 738-740 ◽  
Author(s):  
Ana Paula de Andrade Aukar ◽  
Eliana Gertrudes de Macedo Lemos ◽  
João Carlos Oliveira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Research Work ◽  
Botanical Variety ◽  
Passiflora Edulis ◽  
Large Variability ◽  
Fruit Species ◽  
Passiflora Edulis Sims

It has been evaluated the genetic variability through the use of RAPD molecular markers on the following passionflower species: Passiflora amethystina, P. caerulea, P. cincinnata, P. coccinea, P. serrato digitata, P. foetida, P. maliformis, P. alata, P. giberti, P. laurifolia, P. macrocarpa, P. nitida, P. setacea, P. suberosa, P. ligularis, P. capsularis, P. edulis Sims and its botanical variety P. edulis Sims f. flavicarpa Deg. In this research work, the analyses of the random amplified polymorphic DNA products (RAPD) were employed to estimate the genetic diversity and the taxonomic linkage within the species above. The total of 21 primers were used in this study which generated 270 different polymorphic products. It was possible to detect that the Passiflora species had shown a similarity of 17,3%, and between Passiflora edulis Sims and Passiflora edulis Sims f. flavicarpa a similarity of 34,35% has been found. The rate of similarity within edulis specie is low, making it clear that a large variability between the yellow and the purple forms exists.

scholarly journals Genetic Variability of Isolates of Ramulispora sorghi From Cáceres-MT, Brazil

2019 ◽  
Vol 11 (10) ◽  
pp. 250
Author(s):  
A. V. M. Duarte ◽  
C. L. Correa ◽  
M. A. A. Barelli ◽  
B. W. Zago ◽  
N. L. Sander ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Polymorphic Information Content ◽  
Relevant Information ◽  
Optimization Method ◽  
Control Measures ◽  
Effective Control ◽  
Breeding Programs ◽  
Mato Grosso ◽  
Average Value

Among the most produced cereals worldwide, sorghum (Sorghum bicolor (L.) Moench), presents low productivity in Brazil, mainly due to the occurrence of diseases, with a prominence of sooty stripe, caused by the fungus Ramulispora sorghi, until then considered a secondary disease, has increased its incidence and severity, especially in conditions of high humidity and temperature. The most efficient means of control is the use of resistant cultivars, in this sense, studies on the genetic variability of the fungus through molecular markers are of great importance for the breeding programs of sorghum. The objective of this work was to evaluate the genetic variability in isolates of Ramulispora sorghi belonging to the mycoteca of the Laboratory of Genetic Resources &amp; Biotechnology of the Universidade do Estado de Mato Grosso, campus of C&aacute;ceres, via molecular markers of the ISSR type. The results indicate the existence of genetic variability among the isolates of R. sorghi. The Polymorphic Information Content (PIC) showed that the primers were classified as medium informational with an average value of 0.27. 71 polymorphic fragments were formed considering the 40 isolates of R. sorghi, which presented the value of k = 2, represent the differentiation of the isolates into two distinct clusters. The genetic dissimilarity measures were estimated by the Coefficient of Nei and Li, where the combination between the isolates B107/16 (15) and B103/15 (16) obtained the smallest magnitude (0,12) and the combinations between isolates B111/16 (2) and S114/15 (33), S316/15 (3) and S114/15 (33), B115/16 (4) and S114/15 (33), B118/16 (6) and S114/15 (33) were more dissimilar (1,00). The &ldquo;UPGMA&rdquo; method provided a breakdown of the 40 isolates into 4 distinct groups. The Cophenetic Correlaction Coefficient (CCC) presented significant value with r = 0,84. The Tocher&rsquo;s optimization method allowed the 40 isolates to be distributed in 10 different groups. These results provide relevant information on the genetic variability among the 40 isolates of R. sorghi analyzed. In addition, they indicate that fungus have a wide genetic diversity, and have been recurring in different regions of Brazil and the world, and thus, larger studies become essential for more effective control measures.

scholarly journals LEAF AND FRUIT CHARACTERISTICS AND GENETIC DIVERSITY OF WILD FRUIT Cerasus prostrata GENOTYPES COLLECTED FROM THE CENTRAL ANATOLIA, TURKEY

2021 ◽  
Vol 20 (2) ◽  
pp. 53-62
Author(s):  
Aydın Uzun ◽  
Mehmet Yaman ◽  
Hasan Pinar ◽  
Batuhan Durmuş Gök ◽  
İsa Gazel
Keyword(s):  
Genetic Diversity ◽  
Leaf Length ◽  
Central Anatolia ◽  
Fruit Characteristics ◽  
Significant Information ◽  
Leaf Characteristics ◽  
Fruit Species ◽  
Wild Fruit ◽  
Deciduous Fruit ◽  
Issr Primers

Cerasus prostrata (Lab.) Ser. is quite widespread in some regions of Turkey. It is a wild and deciduous fruit species. The species is commonly encountered in Central Anatolia over the foothills of Erciyes Mountain. In this study, some fruit and leaf characteristics of 30 C. prostrata genotypes collected from the foothills of Erciyes Mountain were determined and genetic diversity among them was presented. Fruit weights of the genotypes varied between 0.66–0.23 g and fruit flesh ratios varied between 84.59–63.11%. Leaf width, leaf length and petiole lengths of the genotypes respectively varied between 1.61–0.68 cm; 4.02–1.82 cm and 0.60–0.28 cm. In genetic analyses, 17 ISSR primers were used and 115 bands were obtained. Of these bands, 98 were polymorphic. All genotypes were distinguished from each other. Relatively high variation was identified between the genotypes and similarity levels varied between 0.70–0.95. Current findings revealed significant information for the preservation and appraisal of C. prostrata. Further studies are recommended for breeding and protection of this species.

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