scholarly journals REGULATION OF MICRORNA EXPRESSION LEVEL BY CHOLERETIC THERAPY IN FUNCTIONAL DISORDERS OF THE GALLBLADDER AND ОDDI’S SPHINCTER IN CHILDREN

2020 ◽  
Vol 73 (1) ◽  
pp. 41-45
Author(s):  
Alexandr E. Abaturov ◽  
Iryna L. Vysochyna ◽  
Veronika L. Babych ◽  
Victor E. Dosenko
Keyword(s):  
Blood Serum ◽  
Ursodeoxycholic Acid ◽  
Standard Therapy ◽  
Microrna Expression ◽  
Healthy Children ◽  
Functional Disorders ◽  
Cellular Mechanisms ◽  
Oddi’S Sphincter ◽  
Polymerase Chain ◽  
Oddi's Sphincter

The alm: To study the effect of choleretic therapy on the level of microRNA expression in functional disorders of the gallbladder and Oddi’s sphincter in children. Materials and methods: Fifty patients with functional disorders of the gallbladder and Oddi’s sphincter who received standard therapy in combination with ursodeoxycholic acid, 20 patients – standard therapy without ursodeoxycholic acid, and 20 healthy children were examined. The level of expression of microRNA-378f, microRNA-4311, microRNA-4714-3p in the blood serum by the method of real-time polymerase chain reaction with reverse transcription according to the protocol TaqMan Gene Expression Assays was performed. Results: It was demonstrated that the activity profile of microRNA-4714-3p was significantly lower in those examined with functional disorders of the gallbladder and Oddi’s sphincter than in practically healthy children (p<0.05). After standard therapy combined with ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter, the level of expression of microRNA-378f is significantly higher than before therapy (5.23±0.70 SU and 2.02±0.57 SU respectively) (p<0.05). Against the background of standard therapy with the addition of ursodeoxycholic acid or without it, the expression profile of microRNA-4714-3p in the blood serum in children with functional disorders of the gallbladder and Oddi’s sphincter significantly decreased (1.93±0.58 SU and 1,14±0,53 SU respectively) (p<0.05). Conclusions: Ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter affects the activity of generation of gene regulators of the cellular mechanisms of microRNA-378f and microRNA-4714-3p.

A New Catheter for Endoscopic Manometry of Oddi's Sphincter

Endoscopy ◽  
1977 ◽  
Vol 9 (01) ◽  
pp. 31-33 ◽  
Author(s):  
W. Rösch ◽  
G. Lux ◽  
K. Seuberth
Keyword(s):  
Endoscopic Manometry ◽  
Oddi’S Sphincter ◽  
Oddi's Sphincter

Streptococcus Mutans Strains in Mother-Child Pairs of Children with Early Childhood Caries

2019 ◽  
Vol 43 (4) ◽  
pp. 252-256 ◽  
Author(s):  
Priya Subramaniam ◽  
Revathy Suresh
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Mutans Streptococci ◽  
Similar Distribution ◽  
Healthy Children ◽  
Only Children ◽  
Polymerase Chain ◽  
Childhood Caries

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

The study of tryptophan metabolite concentrations in blood serum and fecal extracts from obese children

2020 ◽  
Vol 66 (6) ◽  
pp. 494-501
Author(s):  
A.V. Shestopalov ◽  
O.P. Shatova ◽  
A.M. Gaponov ◽  
N.E. Moskaleva ◽  
S.A. Appolonova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Metabolic Activation ◽  
Fold Increase ◽  
Kynurenine Pathway ◽  
Healthy Children ◽  
Obese Children ◽  
Fecal Matter ◽  
Metabolic Coupling ◽  
Tryptophan Metabolites ◽  
Tryptophan Metabolite

We found that changes in the concentrations of tryptophan metabolites in the blood serum and in the intestinal contents are one of the mechanisms for the formation of metabolic coupling in the system "macroorganism-intestinal microbiota", which undergoes significant changes in the development of obesity. Although blood kynurenine remained basically unchanged in obese children we found an increase in some of its serum metabolites: anthranilic, kynurenic and xanthurenic acids. It is noteworthy that in the analysis of fecal matter in obese children, revealed a 2-fold increase in the level of kynurenine while the concentration of kynurenine pathway metabolites corresponded to the level of the group of healthy children. This may indicate the metabolic activation of the microbiota associated with the intestinal mucosa. This is also supported by the absence of statistically significant differences in the concentration of indole in healthy children and in obese children in fecal analyses, and a significant increase in the concentration of indole-3-lactate and indole-3-acetate in the blood serum of obese children.

The choledochoduodenal junction in the dog ? a restudy of Oddi's sphincter

1955 ◽  
Vol 97 (3) ◽  
pp. 431-459 ◽  
Author(s):  
E. P. Eichhorn ◽  
E. A. Boyden
Keyword(s):  
Oddi’S Sphincter ◽  
Oddi's Sphincter

scholarly journals USE OF MATHEMATICAL MODELS INFRARED SPECTROSCOPY SERUM IN THE DIFFERENTIAL DIAGNOSIS OF ULCERATIVE COLITIS AND CROHN'S DISEASE IN CHILDREN

2013 ◽  
Vol 68 (12) ◽  
pp. 44-48
Author(s):  
E. N. Fedulova ◽  
A. S. Gordetsov ◽  
O. V. Fedorova ◽  
L. V. Korkotashvili ◽  
O. A. Tutina
Keyword(s):  
Mathematical Modeling ◽  
Ulcerative Colitis ◽  
Differential Diagnosis ◽  
Crohn’S Disease ◽  
Infrared Spectroscopy ◽  
Crohn's Disease ◽  
Blood Serum ◽  
Malignant Tumors ◽  
Spectral Characteristics ◽  
Healthy Children

Relevance of research. Inflammatory bowel diseases are among the most severe pathologies in pediatric gastroenterology, often lead to disability. Despite the similarity of the clinic, pathogenetic mechanisms, the question of the differential diagnosis of ulcerative colitis and Crohn's disease is relevant in view of their different prognosis and treatment strategy. In recent years, in medical uses infrared spectroscopy of blood serum for the differential diagnosis of various inflammatory diseases, benign and malignant tumors. Besides finding increasing application of mathematical methods for data processing, the so-called mathematical modeling of pathological processes, allowing objectify the survey results for a more accurate diagnosis and prognosis of pathological processes. Objective: improving the differential diagnosis of ulcerative colitis and Crohn's disease in children. Patients: 21 children with ulcerative colitis, 56 children with Crohn's disease and 34 healthy children. The method of infrared spectroscopy of serum and mathematical modeling results through multivariate entropy analysis. Results: the obtained spectral characteristics of blood serum in children with ulcerative colitis and Crohn's disease and in healthy children, as well as "images of disease" in these pathologies. Conclusion: The use of this medical technology reduced the time of diagnosis, which contributes to the timely choice of rational treatment strategies and provides an opportunity to avoid the development of complications, worsening of the disease. 

scholarly journals The Content Of Vitamin D Metabolites In Rachit In Children Of Early Age Who Received Specific Prevention

2021 ◽  
Vol 03 (06) ◽  
pp. 131-138
Author(s):  
Rasuljon Mamasiddikovich Shermatov ◽  
◽  
Zebo Xamroevna Nishanova ◽  
Mavlonjon Ziemiddinovich Nasirdinov ◽  
Shoxrux Ravshanjon Ogli Xabibullayev ◽  
...  
Keyword(s):  
Vitamin D ◽  
Blood Serum ◽  
Kidney Diseases ◽  
Early Age ◽  
Healthy Children ◽  
Vitamin D Metabolites ◽  
Clinical Form ◽  
Liver And Kidney ◽  
Complicated Course ◽  
25 Oh Vitamin D

52 patients with rickets and 10 healthy children were examined. Among patients with rickets, 35 children were born in women with a complicated course of pregnancy (threat of termination, toxicosis of the first and second half). In 12 children, mothers had chronic liver and kidney diseases before giving birth. The level of 25(OH) vitamin D (25-oxycalciferol) and 24.25 (OH)2 vitamin D (24.25-dioxycalciferol) was determined. The content of calcium-regulating hormones in the blood serum was determined by radioimmunological analysis. To assess the severity of the rickets process, the activity of alkaline phosphatase in the blood serum was determined using a standard kit. The aim of our study was to determine the effect of specific prevention on the metabolism and course of the clinical form of rickets in young children.

scholarly journals microecological and associative structure of intestinal biocenosis in children with functional gastrointestinal disorders

2016 ◽  
Vol 1 (5) ◽  
pp. 22-25
Author(s):  
Немченко ◽  
Ulyana Nemchenko ◽  
Савелькаева ◽  
Marina Savelkaeva ◽  
Колесникова ◽  
...  
Keyword(s):  
Large Intestine ◽  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
Healthy Children ◽  
Functional Disorders ◽  
Associative Structure ◽  
Intestinal Microbial Community ◽  
One Year ◽  
Micro Organisms ◽  
And Staphylococcus Aureus

The article is devoted to one of actual problems of gastroenterology – study of microbiocenosis of gastrointestinal tract in functional disorders. Functional gastrointestinal disorders (FGID) or minimal dysfunctions of digestion are frequent in the first months of child’s life and provoke anxiety both in parents and in pediatricians. Aim: to explore the microbial landscape and the associative structure of the microbiota of the large intestine in children with functional disorders of the gastrointestinal tract.Subjects and methods: Intestinal microbiota composition of 225 children with functional gastrointestinal disorders and 100 healthy children was studied using bacteriological method.Results. The 88.4 % frequency of detection of intestinal eubiosis disorders is discussed. Information is provided on the species composition of the major opportunistic organisms that inhabit this biotope. According to the results, decrease of bifidobacteria amount in structure of large intestine microbiota increases rate of detection of opportunistic micro-organisms – predominantly, Klebsiella genus bacteria and Staphylococcus aureus. The intestinal microbial community is dominated by multicomponent transient association. The frequency of detection of pathogenic Staphylococcus in children under one year is discussed in detail.Conclusions. A distinctive feature of the intestinal biocenosis microecological status is significantly greater spread of enterococci than in healthy children, which can be a risk for the emergence of strains with the presence of a number of pathogenecity factors that cause infectious processes.

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