The Combination of Piper Caninum Blume and Piper Betle Var. Nigra Leaf Extract to Press Blast Disease on Oryza Sativa Linn in Vitro

2019 ◽  
Vol 50 (4) ◽  
pp. 196-204
Author(s):  
NI LUH SURIANI ◽  
DEWA NGURAH SUPRAPTA ◽  
KHAMDAN KHALIMI ◽  
ANAK AGUNG KETUT DARMADI ◽  
NI MADE SUSUN PARWANAYONI
Keyword(s):  
Oryza Sativa ◽  
Leaf Extract ◽  
Blast Disease ◽  
Piper Betle

scholarly journals Piper caninum blume leaf extract and compost to suppress blast disease and increase the production of bali red rice (oryza sativa) in green house

2019 ◽  
Vol 5 (4) ◽  
pp. 46-54 ◽  
Author(s):  
Ni Luh Suriani
Keyword(s):  
Oryza Sativa ◽  
Crop Yield ◽  
Leaf Extract ◽  
Organic Fertilizer ◽  
Field Research ◽  
Blast Disease ◽  
Red Rice ◽  
The Right ◽  
The Impact ◽  
The Village

The parameters developed in organic farming are the use of vegetable pesticides and compost and liquid organic fertilizer. Some research has been done but it needs deeper research to get the right product. The purpose of this study was to determine the inhibitory activity of Piper caninum extract against the fungus Pyricuaria oryzae causing blast disease in rice, and to find out how much the impact of compost on increased crop yield after being combined with the extract. This research is a field research located in the village of Senganan, Penebel Tabanan Bali. With the following treatment Fo = control (100% soil). F1 = (soil 90% + compost 10% + extract 0.5%). F2 = (soil 80% + compost 20% + extract 1%) F3 = (soil 70% + 30% + extract 1.5%). F4 = (soil 60% + compost 40% + 2% extract), do repeat 4 times. The results showed that P. caninum extract effectively inhibited blast disease and could reduce the intensity of blast disease. The combination with compost can significantly increase yields (67.62%), reduce empty grains, and increase rice size.

scholarly journals Study on antifungal potency of Terminalia cattapa, Piper betle, Psidium guajava, and Andrographis peniculata on the growth of Aphanomyces in vitro

2007 ◽  
Vol 4 (2) ◽  
pp. 115
Author(s):  
Sri Nuryati ◽  
. Rahman ◽  
. Taukhid
Keyword(s):  
Leaf Extract ◽  
Psidium Guajava ◽  
Aquatic Fungi ◽  
Piper Betle ◽  
Natural Material ◽  
Leaf Extracts ◽  
Prevention Activity

<p>An effort to prevent aquatic fungi  <em>Aphanomyces</em> sp. infection on fish using natural material can be an economically way, easy to find the materials, easy to apply and safe for environment.  The antifungal potency and efficacy of scalded-leaf extract of <em>Terminalia cattapa</em>, <em>Piper betle</em>, <em>Psidium guajava</em> and <em>Andrographis peniculata</em> on prevention of <em>Aphanomyces</em> sp. growth in vitro in GYA medium.  Scalding was performed in the water at 50°C. Concentration of leaf extracts tested was 0, 10, 20, 40 and 80 g/L.  The results of study showed that <em>Terminalia cattapa</em> in a dosage of 40 g/L had the best prevention activity, followed by <em>Piper betle</em> in the same dosage.  <em>Psidium guajava</em> and <em>Andrographis peniculata</em> had no prevention activity on growth of <em>Aphanomyces</em> sp.</p> <p>Keywords: antifungal, <em>Terminalia cattapa</em>, <em>Piper betle</em>, <em>Psidium guajava</em>, <em>Andrographis peniculata</em> growth, <em>Aohanomyces</em> sp.</p> <p> </p> <p>ABSTRAK</p> <p>Upaya penanggulangan infeksi cendawan akuatik <em>Aphanomyces</em> sp. pada ikan menggunakan bahan alami dapat menjadi cara yang ekonomis ekonomis, bahan mudah didapat, mudah diterapkan dan aman bagi lingkungan. Potensi antifungi dan efektivitas ekstrak seduh daun ketapang (<em>Terminalia cattapa</em>), sirih<em> </em>(<em>Piper betle</em>), jambu biji (<em>Psidium guajava</em>) dan sambiloto (<em>Andrographis peniculata</em>) terhadap penghambatan pertumbuhan <em>Aphanomyces </em>sp. dilakukan secara <em>in vitro</em> dalam media biakan GYA. Penyeduhan dilakukan menggunakan pelarut air dengan suhu 50°C. Konsentrasi yang diuji adalah 0, 10, 20, 40 dan 80 gr/L untuk masing-masing bahan. Aktivitas penghambatan paling baik terhadap cendawan diperoleh dari ekstrak seduh daun ketapang 40 g/L dan diikuti oleh ekstrak seduh daun sirih dengan konsentrasi yang sama.  Jambu biji dan sambiloto tidak menunjukkan aktivitas penghambatan terhadap pertumbuhan <em>Aphanomyces</em> sp.</p> <p>Kata kunci: antifungi, ketapang, sirih, jambu biji, sambiloto dan A<em>phanomyces</em> sp.</p>

scholarly journals IDENTIFICATION OF ANTHRACHNOSE DISEASE ON STRAWBERRY FRUIT (Fragraria vesca L.) AND ITS CONTROL BY BETEL (Piper betle L.) LEAF EXTRACT

2015 ◽  
Vol 2 (1) ◽  
pp. 458
Author(s):  
Rina Sri Kasiamdari ◽  
Umi Sangadah
Keyword(s):  
Disease Severity ◽  
Leaf Extract ◽  
Piper Betle ◽  
Strawberry Fruit ◽  
Pathogen Infection ◽  
Anthracnose Disease ◽  
Betel Leaf ◽  
Colletotrichum Spp

<p>In Indonesia, strawberry is one of fruits which has high economic values. The growth of strawberry has increased significantly, but its productivity is still low because of disease attack. Disease that attacks strawberry started from nursery to post harvest is anthracnose that can be caused by Colletotrichum spp. Control efforts of anthracnose disease up to now has been done with application of synthetic chemicals which are known to be hazardous to the environment and human health. Betel leaf (Piper betle L.) is natural plant that potentially used as phytofungicide. Essential oils on betel leaf extract is reported can decrease the growth of pathogenic fungi. The objectives of the research were to identify Colletotrichum spp. in strawberry fruit that showed anthracnose symptoms, to test the potency of betel leaf extract as phytofungicide, and to evaluate the effect of betel leaf extract in inhibiting infection and disease severity of Colletotrichum in strawberry. Samples were taken from strawberry nursery in Magelang, Indonesia. The disease symptoms were isolated on Potato Dextrose Agar, followed by identification of fungal colony and morphology by semi permanent slide preparation. Betel leaves were extracted with water to get a concentration of 20, 40, 60, 80 and 100%, respectively. An in vitro experiment was done to evaluate the inhibition rate of betel leaf extract to the growth of Colletotrichum spp. colony. An in vivo experiment was done by submersion of strawberry to betel leaf extract before (preventive method) and after antrachnose attack (curative method), then disease infection and disease severity were measured after 7 days. The result of the research showed that anthracnose disease in strawberry was caused by C. gloeosporioides (Penz.). Twenty percent of betel leaf extract in an in vitro test had potential as phytofungicide and concentration of 100% betel leaf extract was the best concentration to inhibit the growth of C. gloeosporioides (Penz.) colony by 70.26±0,61%. In an in vivo experiment, submersion of strawberry before pathogen infection (preventive) with 100% betel leaf extract decreased disease infection by 40% and disease severity by 16%, respectively. While treatment of submersion of strawberry after pathogen infection (curative) decreased disease infection by 33,4% and disease severity by 17%. </p><p>Keywords: strawberry, anthrachnose, disease, betel leaf</p>

scholarly journals Uji Aktivitas Antifungi Ekstrak Daun Sirih (Piper betle L) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activities Test Of Betel Leaf Extract (Piper betle L) On Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
Rahayu Kusdarwati, Pustika Murtinintias, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Piper Betle ◽  
Minimum Concentration ◽  
Minimum Fungicidal Concentration ◽  
Betel Leaf ◽  
Concentration Test ◽  
Fungal Suspension

Abstract Saprolegniasis is a mycotic disease caused by Saprolegnia sp. that usually attacking wild fish and farming fish. Saprolegnia sp. cause a lot of harm in process of the fish cultivation. Prevention and treatment of the common practice is use chemical drugs, but the use of these chemicals tend to be environmentally unfriendly and there are has karsinogenik effect. Therefore, the use of traditional medicines is one of alternative to control Saprolegnia sp. safer than chemical drugs. Green betel leaf contains phenolic compounds and tannins are efficacious as antifungal agent. This study aims to prove the antifungal activity of extracts of betel leaf (Piper betle L) for Saprolegnia sp., and to know the minimum concentration of betel leaf extract (Piper betle L) as antifungal for Saprolegnia sp. The design of this experiment is used completely randomized design (CRD) with 11 treatments and 3 replications. This study used the dilution method through the Minimum Inhibitory Concentration determination (MIC) and Minimum Fungicidal Concentration (MFC). The concentration of the extract used was 50% (0.5 g/ml), 25% (0.25 g/ml), 12.5% (0.125 g/ml), 6.25% (0.0625 g/ml), 3.13% (0.0313 g/ml), 1.56% (0.0156 g/ml), 0.78% (0.0078 g/ml), 0.39% (0.0039 g/ml), 0.2% (0.002 g/ml) of betel leaf extract. A positive control containing 2 ml of 3% hydrogen peroxide were added fungal suspension until 4 ml Negative control containing 2 ml of 10% DMSO were added fungal suspension until 4 ml. The main parameters in this study is the value of optical density (OD) for MIC (Minimum Inhibitory Concentration) test and the absence of Saprolegnia sp. growing on SDA media for MFC (Minimum Fungicidal Concentration) test. 0.20% (0.002g/ml) concentration of of betel leaf extract is the minimum concentration that can inhibit the growth of Saprolegnia sp. MFC test results showed concentrations of 0.78% (0.0078 g/ml) betel leaf extract is the minimum concentration that can kill Saprolegnia sp.

Antioxidant activity of Piper betle L. leaf extract in vitro

2004 ◽  
Vol 88 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Nabasree Dasgupta ◽  
Bratati De
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Piper Betle

scholarly journals Influence of phytochemicals in piper betle linn leaf extract on wound healing

2015 ◽  
Vol 3 ◽  
pp. 1-8 ◽  
Author(s):  
Le Thi Lien ◽  
Nguyen Thi Tho ◽  
Do Minh Ha ◽  
Pham Luong Hang ◽  
Phan Tuan Nghia ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Lipid Hydroperoxide ◽  
Piper Betle ◽  
Burn Wound ◽  
Nih3t3 Cells ◽  
Cutaneous Wounds ◽  
Scratch Wound

Abstract Background Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. Methods Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment. Results The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice. Conclusions Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.

scholarly journals UJI IN VITRO AKTIVITAS ANTIBAKTERI DARI DAUN SIRIH

2013 ◽  
Vol 2 (3) ◽  
Author(s):  
Oksfriani J. Sumampouw
Keyword(s):  
Analysis Of Variance ◽  
Leaf Extract ◽  
Piper Betle ◽  
Chemical Laboratory ◽  
E Coli ◽  
North Sulawesi ◽  
Significant Difference ◽  
Advanced Science ◽  
Drug Resistant Strains

Abstract: Diarrhea remains one of the global problems, especially in developing countries such as Indonesia. Due to drug resistant strains, new antibacterial drugs are needed to be developed. The leaf extract of piper betle from a common fruit tree in Indonesia, including Minahasa in North Sulawesi Province, is used by the Minahasans for treating diarrhea. This leaf extract has inhibitive effects on populations of bacteria and parasites. The aim of this research was to prove the antibacterial effect of piper betle leaves on Escherichia coli. This research was conducted from December 2007 to June 2008 in the Fish Product Microbiology Laboratory of the Fisheries and Marine Science Faculty at Sam Ratulangi University, the Advanced Science Laboratory of Sam Ratulangi University, and the Chemical Laboratory of Mathematics and Natural Sciences Faculty of Sam Ratulangi University. This was an in vitro experimental research. The statistical test used to verify the effectiveness of piper betle extract as an antibacterial to E. coli was the Analysis of Variance (ANOVA), followed by the Least Significant Difference test (LSD). The conclusion of this research was that the extract of piper betle leaves had antibacterial activity on E.coli. Keywords: Piper betle leaf, antibacterial.  Abstrak: Diare masih menjadi sebuah masalah global, terutama pada negara negara berkembang, termasuk Indonesia, dan khususnya Minahasa. Hal ini disebabkan beberapa strain tertentu penyebab diare seperti Escherchia coli telah resisten terhadap obat-obatan. Oleh karena itu merupakan keniscayaan ditemukan senyawa baru sebagai antibakteri untuk mengobati diare. Ekstrak daun sirih yang umumnya ada di Indonesia, juga di  Minahasa, telah sering digunakan sebagai obat anti diare. Ekstrak daun ini telah ditemukan dapat menghambat populasi bakteri dan parasit. Penelitian ini bertujuan untuk melihat aktivitas antibakteri ekstrak daun sirih terhadap E. coli. Penelitian ini dilaksanakan di Laboratorium Mikrobiologi Hasil Perikanan Fakultas Perikanan dan Ilmu Kelautan Universitas Sam Ratulangi Manado, Laboratorium Advanced Science Universitas Sam Ratulangi Manado dan Laboratorium Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi Manado pada bulan Desember 2007 sampai Juni 2008. Penelitian ini merupakan penelitian eksperimental secara in vitro. Uji statistika efektifitas antibakteri dari ekstrak daun sirih terhadap E. coli menggunakan Analysis of Variance (ANOVA), dan dilanjutkan dengan uji Least Significant Difference (LSD). Hasil penelitian ini memperlihatkan bahwa ekstrak daun P. betle mengan-dung senyawa yang potensial untuk menghambat pertumbuhan E. coli Kata Kunci: daun sirih, anti bakteri.

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