EFEK IRADIASI SINAR GAMMA PADA TANAMAN KACANG PENUTUP TANAH (Mucuna bracteata L.)

Legume cover crop (Mucuna bracteata L.) is a creeper which is currently often used to increase soil fertility in plantation areas. This plant is a leguminous plant that can fix nitrogen nutrients in the soil. One of the M.bracteata plant breeding programs to increase diversity is mutation using gamma ray radiation. The research objective was to determine the level of radiosensitivity of legume cover crop using gamma ray irradiation. The research was conducted at PAIR BATAN using a Gamma Chamber 4000A irradiator and in agricultural experiment, Labuhanbatu University. The plant material used was M.bracteata seeds with irradiation doses of 0, 200, 250, 300, 350 and 400 Gy. This study uses a curve fit analysis program to calculate the LD50 value (Lethal dose 50). The results showed that the plant radiosensitivity by calculating the LD50 value of M.bracteata at the age of 2 Weeks After Planting) was 348.737 Gy. The higher the radiation dose given, the lower the percentage of growth. Key words: diversity; leguminous; lethal dosage; mutation; gamma rays

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PENGARUH IRADIASI SINAR GAMMA [60Co] TERHADAP BACTROCERA CARAMBOLAE DREW & HANCOCK IN VITRO DAN IN VIVO

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.11517-25 ◽

2015 ◽

Vol 15 (1) ◽

pp. 17

Author(s):

Endang Sri Ratna ◽

Kemas Usman ◽

Indah Arastuti ◽

Dadan Hindayana

Keyword(s):

Gamma Ray ◽

Lethal Dose ◽

Fruit Fly ◽

Probit Analysis ◽

Adult Survival ◽

Significance Level ◽

Bactrocera Carambolae ◽

Gamma Ray Irradiation

Effect of gamma irradiation [60Co] against Bactrocera carambolae Drew & Hancock in vitro and in vivo. Bactrocera carambolae Drew & Hancock is one of the most important pests on guava fruit. According to a quarantine regulation in export-import commodities, irradiation treatment is a suitable methods for eradicating infested organism, which is relatively safe for the environment. The aim of this research was to determine mortality doses and an effective dose of [60Co] gamma ray irradiation for the eradication purpose, and its implication on the survival of fruit fly B. carambolae. Two irradiation methods of in vitro dan in vivo were carried out, by exposing egg and 3rd instar larvae of B. carambolae obtained from the laboratory reared insect. Eleven doses of gamma ray irradiation of 0, 30, 50, 75, 100, 125, 150, 175, 200, 300, 450, and 600 Gy were applied, respectively. The level of 99% fruit fly mortality was estimated by the value of LD99 using probit analysis and the number of larvae, pupae and adult survival were evaluated by analysis of variance (ANOVA), and the means compared by Tukey’s test, at 5% of significance level. These result showed that the effective lethal dose (LD99) of irradiation that could be successful to eradicate eggs and 3rd instar larvae in vitro were 2225 and 2343 Gy and in vivo were 3165 dan 3177 Gy, respectively. Almost all of the treated larvae survived and developed to pupae, therefore only the minimum irradiation dose of 30 Gy allowed the pupae to develop into adults.

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Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation

Journal of Tropical Crop Science ◽

10.29244/jtcs.2.1.26-32 ◽

2015 ◽

Vol 2 (1) ◽

pp. 26-32

Author(s):

Syarifah Iis Aisyah ◽

Yodi Marthin ◽

M. Rizal M. Damanik

Keyword(s):

Gamma Irradiation ◽

Dose Level ◽

Gamma Ray ◽

Lethal Dose ◽

Mutation Induction ◽

Coleus Blumei ◽

Irradiation Treatment ◽

Gamma Ray Irradiation ◽

Number Of Leaves ◽

Short Time

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords: Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun

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Collimated Microbeam Reveals that the Proportion of Non-Damaged Cells in Irradiated Blastoderm Determines the Success of Development in Medaka (Oryzias latipes) Embryos

Biology ◽

10.3390/biology9120447 ◽

2020 ◽

Vol 9 (12) ◽

pp. 447

Author(s):

Takako Yasuda ◽

Tomoo Funayama ◽

Kento Nagata ◽

Duolin Li ◽

Takuya Endo ◽

...

Keyword(s):

Brain Development ◽

Gamma Rays ◽

Low Dose ◽

Gamma Ray ◽

Lethal Dose ◽

Carbon Ion ◽

Embryonic And Fetal Development ◽

Developmental Disturbance ◽

Gamma Ray Irradiation ◽

Neurula Stage

It has been widely accepted that prenatal exposure to ionizing radiation (IR) can affect embryonic and fetal development in mammals, depending on dose and gestational age of the exposure, however, the precise machinery underlying the IR-induced disturbance of embryonic development is still remained elusive. In this study, we examined the effects of gamma-ray irradiation on blastula embryos of medaka and found transient delay of brain development even when they hatched normally with low dose irradiation (2 and 5 Gy). In contrast, irradiation of higher dose of gamma-rays (10 Gy) killed the embryos with malformations before hatching. We then conducted targeted irradiation of blastoderm with a collimated carbon-ion microbeam. When a part (about 4, 10 and 25%) of blastoderm cells were injured by lethal dose (50 Gy) of carbon-ion microbeam irradiation, loss of about 10% or less of blastoderm cells induced only the transient delay of brain development and the embryos hatched normally, whereas embryos with about 25% of their blastoderm cells were irradiated stopped development at neurula stage and died. These findings strongly suggest that the developmental disturbance in the IR irradiated embryos is determined by the proportion of severely injured cells in the blastoderm.

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Pengaruh Iradiasi Sinar Gamma terhadap Pertumbuhan Stek Tunas Apikal dan Aksilar Kopi Arabika

Jurnal Tanaman Industri dan Penyegar ◽

10.21082/jtidp.v7n3.2020.p137-148 ◽

2020 ◽

Vol 7 (3) ◽

pp. 137

Author(s):

Meynarti Sari Dewi Ibrahim ◽

Enny Randriani

Keyword(s):

Genetic Diversity ◽

Gamma Ray ◽

Energy Agency ◽

Arabica Coffee ◽

Apical Shoot ◽

Low Genetic Diversity ◽

Planting Material ◽

Randomized Design ◽

Gamma Ray Irradiation ◽

Ld50 Value

The Arabica coffee is predominantly self-pollinated plants thereby contributing to low genetic diversity. The effort to increase the genetic diversity of Arabica coffee through crossing strategy is time-consuming, and induce mutation is necessary to enhance the rate of genetic variation. The aims of this study were to observe the effect of gamma-ray irradiation on the growth of apical and axillary bud cuttings and to determine the value of LD50 on apical cuttings and Arabica coffee axillaries. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute, from January to December in 2018.. The planting material that was irradiated was Arabica coffee plantlets resulting from somatic embryogenesis propagation. Irradiation is carried out at the National Nuclear Energy Agency. The irradiated plantlets were cut and subcultured onto MS medium without growth regulators, 30 g L-1 sucrose, and 2.5 g L-1 phytagel were added. The design used a completely randomized design with 10 replications. The treatments tested were the dose of gamma-ray irradiation (0, 10, 20, 30, 40, and 50 Gy). The results showed that gamma-ray irradiation had an effect on all observed parameters. The mortality percentage of apical shoot cuttings began to be found at 30 Gy, while axillary cuttings at 20 Gy increased with an increasing dose of gamma-ray irradiation. The number of shoots and leaves varied between irradiation doses on both apical and axillary cuttings. The LD50 value of apical shoot cuttings was 36.80 Gy, while axillary cuttings were 22.24 Gy

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Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v22i2.44151 ◽

2020 ◽

Vol 22 (2) ◽

pp. 105

Author(s):

Siti Hartati Yusida Saragih ◽

Khairul Rizal ◽

Kamsia Dorliana Sitanggang

Keyword(s):

Genetic Diversity ◽

Gamma Ray ◽

Lethal Dose ◽

Mucuna Pruriens ◽

Mutation Induction ◽

High Genetic Diversity ◽

Group Design ◽

Planting Material ◽

Gamma Ray Irradiation ◽

Number Of Leaves

Breeding kara benguk with mutation induction techniques using gamma ray radiation is expected to produce genetic diversity. The purpose of the study is to determine the value of Lethal Dose 50 (LD50) and obtain the genetic diversity of the kara benguk plant. The research was conducted at PAIR BATAN and Agrotechnology experiment, Universitas Labuhanbatu from May-July 2020. The planting material used was kara benguk seeds. The method used was Randomized Complete Group Design (RCGD) with one factor which was dose of irradiation with six levels (0, 200, 250, 300, 350 and 400 Gy) and three replications. Each replication consisted of 20 plants, so there are a total of 360 experimental plants. The results showed that the value of LD50 kara benguk in 3 weeks after planting was 281,472 Gy. Gamma ray irradiation increases the high genetic diversity of plants and the number of leaves at a dose of 350 Gy.

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Radiosensitivitas Kalus Embriogenik Kopi Robusta Bp 436 Terhadap Iradiasi Sinar Gamma

Jurnal Tanaman Industri dan Penyegar ◽

10.21082/jtidp.v6n1.2019.p41-50 ◽

2019 ◽

Vol 6 (1) ◽

pp. 41

Author(s):

Meynarti Sari Dewi Ibrahim ◽

Enny Randriani ◽

Laela Sari ◽

Anne Nuraini

Keyword(s):

Genetic Diversity ◽

Somatic Embryos ◽

Gamma Ray ◽

Lethal Dose ◽

Mutation Induction ◽

High Genetic Diversity ◽

Robusta Coffee ◽

Randomized Design ◽

Young Leaves ◽

Gamma Ray Irradiation

High genetic diversity is one factor that determines the success of plant breeding. Mutation induction by gamma ray irradiation is one method to improve plant genetic diversity. This study aimed to 1) obtain growth regulators composition suitable in inducing embryogenic callus, 2) determine the effect of gamma ray irradiation on the growth and development of somatic embryos, and 3) obtain lethal dose (LD) 20 and 50 values in Robusta coffee BP 436. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute, from May 2017 to December 2018. Explants used were young leaves of Robusta coffee BP 436. Callus induction used ½ MS media with 2,4-D (4.52 μM) and 2-iP (0.00; 4.93; 9.86; 14.79; and 19.72 μM) treatment. Mutation induction was performed using gamma radiation dosed at 0, 10, 20, 30, 40, and 50 Gy treatments. The regeneration media was ½ MS containing GA3 (0 and 1 mg/l). The study used a complete randomized design with 10 replications. The results showed the best combination of plant growth regulator to induce the callus was 2.4-D 4.52 μM + 2-iP 19.72 μM. The fresh weight of cultures was inhibited above 30 Gy, whereas the number of somatic embryos decreased at doses above 10 Gy. Addition of GA3 1 mg/l in regeneration media increased the number of somatic embryos in torpedo phase, but not in gamma irradiation exposed calluses. The LD20 and LD50 of Robusta coffee BP 436 are 16.81 and 28.52 Gy, respectively.

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