scholarly journals Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation

2015 ◽  
Vol 2 (1) ◽  
pp. 26-32
Author(s):  
Syarifah Iis Aisyah ◽  
Yodi Marthin ◽  
M. Rizal M. Damanik
Keyword(s):  
Gamma Irradiation ◽  
Dose Level ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
Coleus Blumei ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Short Time

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun

scholarly journals Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

2020 ◽  
Vol 22 (2) ◽  
pp. 105
Author(s):  
Siti Hartati Yusida Saragih ◽  
Khairul Rizal ◽  
Kamsia Dorliana Sitanggang
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Group Design ◽  
Planting Material ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

<p>Breeding kara benguk with mutation induction techniques using gamma ray radiation is expected to produce genetic diversity. The purpose of the study is to determine the value of Lethal Dose 50 (LD50) and obtain the genetic diversity of the kara benguk plant. The research was conducted at PAIR BATAN and Agrotechnology experiment, Universitas Labuhanbatu from May-July 2020. The planting material used was kara benguk seeds. The method used was Randomized Complete Group Design (RCGD) with one factor which was dose of irradiation with six levels (0, 200, 250, 300, 350 and 400 Gy) and three replications. Each replication consisted of 20 plants, so there are a total of 360 experimental plants. The results showed that the value of LD50 kara benguk in 3 weeks after planting was 281,472 Gy. Gamma ray irradiation increases the high genetic diversity of plants and the number of leaves at a dose of 350 Gy.</p>

scholarly journals Induksi Mutasi Fisik dengan Iradiasi Sinar Gamma pada Kacang Merah

2017 ◽  
Vol 1 (1) ◽  
pp. 49
Author(s):  
Siti Marwiyah ◽  
Heny Purnamawati ◽  
Pinni Iyunika Sembiring
Keyword(s):  
Gamma Ray ◽  
Mutation Induction ◽  
Seed Color ◽  
Color Code ◽  
Kidney Beans ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Four Levels ◽  
Red Bean ◽  
Dose Change

Physical mutation induction by gamma ray irradiation can increase the genetik diversity to support the selection ofcertain characters in plant breeding. Varieties is the key to improving the productivity of kidney beans (Phaseolus vulgaris) in Indonesia. This study aimed to study the effect of physical mutation induction by gamma ray irradiation of the red bean genotypes Brast-1. The research was conducted from February to May 2016. The genetik material Brast genotype-1 was irradiated with four levels of gamma ray (55, 110, 165 and 220 Gy). Weight of 100 grains M1 at a dose of 55 Gy were significantly different from M0 is a positive change from the gamma ray irradiation treatment. Effect of gamma ray irradiation was indicated by the changing of the morphology of leaves, pods and seed color code of M2.Keywords: dose, change, genotype, M2 seed

scholarly journals Pengaruh Mutasi Fisik Iradiasi Sinar Gamma terhadap Keragaman Genetik dan Penampilan Coleus blumei

2016 ◽  
Vol 7 (3) ◽  
pp. 187
Author(s):  
Eny Rolenti Togatorop ◽  
Syarifah Iis Aisyah ◽  
M. Rizal M. Damanik
Keyword(s):  
Genetic Variability ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Ornamental Plant ◽  
Fractionated Irradiation ◽  
Coleus Blumei ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Putative Mutant ◽  
Number Of Branches

<p>ABSTRACT<br />Mutation breeding such as gamma ray irradiation is one of strategy to increase genetic variability. The aim of this research was to indentify genetic variability, performance changes and to obtain putative mutant of Coleus blumei purple/green through gamma ray irradiation. The experiment design used was Randomized Complete Block with single factor and three replications. The gamma ray irradiation was given to shoot cuttings of C. blumei by fractionated irradiation dose: 0 Gy (control), 20+20 Gy, 22.5+22.5 Gy, 25+25 Gy and 27.5+27.5 Gy. The irradiated shoot cuttings were planted in field until MV3 generation. The result of this research showed that gamma ray irradiation on C.blumei purple/green produced the high genetic variability on number of leaves and number of branches i.e. 58.48% and 74.02% by 25+25 Gy dose and number of branches by 20+20 Gy and 22.5+22.5 Gy dose i.e. 53.47% and 68.97% respectively. Physically induced mutation by gamma ray irradiation produced 5 putative mutants respectively on colour and pattern of leaf changes in the following plants: 20+20.5, 20+20.7, 22.5+22.5.8, 25+25.5 and 25+25.8.<br />Keywords: fractionated irradiation, mutagen, ornamental plant, putative mutan, shoot cutting</p><p>ABSTRAK<br />Pemuliaan mutasi dengan iradiasi sinar gamma merupakan salah satu cara dalam meningkatkan keragaman genetik tanaman. Tujuan penelitian ini untuk mengidentifikasi keragaman genetik, perubahan penampilan dan mendapatkan mutan putatif pada tanaman Coleus blumei ungu/hijau melalui iradiasi sinar gamma. Penelitian menggunakan rancangan kelompok lengkap teracak (RKLT) faktor tunggal dengan 3 ulangan. Iradiasi sinar gamma diberikan terhadap stek pucuk C. blumei ungu/hijau dengan dosis terbagi yaitu: 0 Gy (kontrol), 20+20 Gy, 22.5+22.5 Gy, 25+25 Gy dan 27.5+27.5 Gy. Semua tanaman hasil iradiasi ditanam di lapangan sampai generasi MV3. Hasil penelitian menunjukkan bahwa pemberian iradiasi sinar gamma pada C. blumei ungu/hijau menghasilkan keragaman genetik yang cukup tinggi pada karakter jumlah daun dan jumlah cabang dengan nilai KKG masing-masing 58.48% dan 74.02% pada dosis 25+25 Gy serta karakter jumlah cabang dengan nilai KKG 53.47% dan 68.97% masing-masing pada dosis 20+20 gy dan 22.5+22.5 Gy. Mutasi induksi fisik dengan iradiasi sinar gamma pada C. blumei ungu/hijau<br />menghasilkan 5 mutan putatif berdasarkan perubahan warna dan corak daun yaitu pada tanaman: 20+20.5, 20+20.7, 22.5+22.5.8, 25+25.5 dan 25+25.8.<br />Kata kunci: iradiasi terbagi, mutagen, mutan putatif, stek pucuk, tanaman hias</p>

scholarly journals Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Sri Hartati ◽  
Prasetyo Prasetyo ◽  
Endang Setia Muliawati
Keyword(s):  
Gamma Irradiation ◽  
Plant Height ◽  
Gamma Ray ◽  
Ornamental Plants ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Leaf Length ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Light Green

<p>Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of <em>Vanda</em> sp. hybrid results gamma irradiation are high at dose 10-40 Gy.</p><p> </p><p>Keywords:<strong> </strong>characteristics; morphological; orchid.</p>

scholarly journals Radiosensitivitas Kalus Embriogenik Kopi Robusta Bp 436 Terhadap Iradiasi Sinar Gamma

2019 ◽  
Vol 6 (1) ◽  
pp. 41
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Enny Randriani ◽  
Laela Sari ◽  
Anne Nuraini
Keyword(s):  
Genetic Diversity ◽  
Somatic Embryos ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Robusta Coffee ◽  
Randomized Design ◽  
Young Leaves ◽  
Gamma Ray Irradiation

<p><em>High genetic diversity is one factor that determines the success of plant breeding. Mutation induction by gamma ray irradiation is one method to improve plant genetic diversity. This study aimed to 1) obtain growth regulators composition suitable in inducing embryogenic callus, 2) determine the effect of gamma ray irradiation on the growth and development of somatic embryos, and 3) obtain lethal dose (LD) <sub>20</sub> and <sub>50</sub> values in Robusta coffee  BP 436. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute,  from May 2017 to December 2018. Explants used were young leaves of Robusta coffee BP 436. Callus induction used ½ MS media with 2,4-D (4.52 μM) and 2-iP (0.00; 4.93; 9.86; 14.79; and 19.72 μM) treatment. Mutation induction was performed using gamma radiation dosed at 0, 10, 20, 30, 40, and 50 Gy treatments. The regeneration media was ½ MS containing GA<sub>3</sub> (0 and 1 mg/l). The study used a complete randomized design with 10 replications. The results showed the best combination of plant growth regulator to induce the callus was 2.4-D 4.52 μM + 2-iP 19.72 μM. The fresh weight of cultures was inhibited above 30 Gy, whereas the number of somatic embryos decreased at doses above 10 Gy. Addition of GA<sub>3</sub> 1 mg/l in regeneration media increased the number of somatic embryos in torpedo phase, but not in gamma irradiation exposed calluses. The LD<sub>20</sub> and LD<sub>50</sub> of Robusta coffee BP 436 are 16.81 and 28.52 Gy, respectively.</em></p>

scholarly journals THE EFFECT OF GAMMA IRRADIATION TO THE PHENOTYPIC OF TWO AGLONEMA VARIETIES

2017 ◽  
Vol 2 (2) ◽  
pp. 21-26
Author(s):  
Arya Widura Ritonga ◽  
Dewi Sukma
Keyword(s):  
Gamma Ray ◽  
Phenotypic Diversity ◽  
Economic Value ◽  
Leaf Length ◽  
Leaf Color ◽  
Group Design ◽  
Irradiation Treatment ◽  
Two Factors ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

Increased phenotypic diversity is needed to increase the economic value of Aglaonema. However, information on increasing phenotypic diversity of Aglaonema using gamma-ray irradiation has not been widely known. This study aimed to investigate the effect of gamma ray irradiation treatment to the performances of two varieties of Aglaonema. This research was arranged factorially using randomized group design (RKLT) of two factors consisting of 8 combinations of treatments that are 4 level of irradiation dose and 2 Aglaonema varieties. The results showed that the induction of gamma ray irradiation decreased the % viable of the plants, the number of leaves, leaf length, leaf width, and the % green color as well as increased the % blue on the leaves of Aglaonema Butterfly and Aglaonema Siam Aurora. The interaction between dose of irradiation and aglaonema varieties was obtained in the % red of leaf color. Both of Aglaonema varieties had a high radiosensitivity with LD50 values ranged of 16.70 - 17.14 Gy

scholarly journals The morphological diversity of Chrysanthemum resulted from gamma ray irradiation

2019 ◽  
Vol 20 (2) ◽  
pp. 463-467 ◽  
Author(s):  
EMI SUSILA ◽  
ARI SUSILOWATI ◽  
AHMAD YUNUS
Keyword(s):  
Gamma Irradiation ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Leaf Length ◽  
Stem Length ◽  
Mutation Induction ◽  
Market Demand ◽  
Morphological Variations ◽  
Color Changes ◽  
Gamma Ray Irradiation

Abstract. Susila E, Susilowati A, Yunus A. 2019. The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas 20: 463-467. Chrysanthemum is a popular ornamental plant. The high market demand makes breeders develop new cultivars to meet market preferences. One way to get superior varieties of Chrysanthemum is by using gamma ray mutation induction which will increase the morphological variations. The purpose of this study is to determine the Chrysanthemum’s morphological diversity resulted from gamma irradiation through morphological markers. This experimental research used a completely randomized design (CRD) with one factor, i.e. radiation doses: 0 Gy (control), 10 Gy, 15 Gy, and 20 Gy. The observations were carried out qualitatively and quantitatively. The data obtained were analyzed using the SPSS 15.0 and the NTSYS (Numerical Taxonomy and Multivariate Analysis System) 2.02 program. The results showed that increasing the dose of gamma irradiation inhibited plant growth. Irradiation at 10 Gy and 20 Gy produced the most color changes in flowers compared to other doses. The basic color of 0 Gy irradiation is purple. Irradiation at 10 Gy and 20 Gy produced dark purple and deep red. Quantitative data showed that the treatment of gamma ray irradiation significantly affected the leaf length, leaf width, stem diameter, stem length, and diameter of flowers. The irradiation also caused changes in shape and texture of the leaves. The dendrogram showed that 20 Gy irradiation resulted the most diverse morphology compared to the control, 10 Gy and 15 Gy.

scholarly journals PENGARUH IRADIASI SINAR GAMMA [60Co] TERHADAP BACTROCERA CARAMBOLAE DREW & HANCOCK IN VITRO DAN IN VIVO

2015 ◽  
Vol 15 (1) ◽  
pp. 17
Author(s):  
Endang Sri Ratna ◽  
Kemas Usman ◽  
Indah Arastuti ◽  
Dadan Hindayana
Keyword(s):  
Gamma Ray ◽  
Lethal Dose ◽  
Fruit Fly ◽  
Probit Analysis ◽  
Adult Survival ◽  
Significance Level ◽  
Bactrocera Carambolae ◽  
Gamma Ray Irradiation

Effect of gamma irradiation [60Co] against Bactrocera carambolae Drew & Hancock in vitro and in vivo. Bactrocera carambolae Drew & Hancock is one of the most important pests on guava fruit. According to a quarantine regulation in export-import commodities, irradiation treatment is a suitable methods for eradicating infested organism, which is relatively safe for the environment. The aim of this research was to determine mortality doses and an effective dose of [60Co] gamma ray irradiation for the eradication purpose, and its implication on the survival of fruit fly B. carambolae. Two irradiation methods of in vitro dan in vivo were carried out, by exposing egg and 3rd instar larvae of B. carambolae obtained from the laboratory reared insect. Eleven doses of gamma ray irradiation of 0, 30, 50, 75, 100, 125, 150, 175, 200, 300, 450, and 600 Gy were applied, respectively. The level of 99% fruit fly mortality was estimated by the value of LD99 using probit analysis and the number of larvae, pupae and adult survival were evaluated by analysis of variance (ANOVA), and the means compared by Tukey’s test, at 5% of significance level. These result showed that the effective lethal dose (LD99) of irradiation that could be successful to eradicate eggs and 3rd instar larvae in vitro were 2225 and 2343 Gy and in vivo were 3165 dan 3177 Gy, respectively. Almost all of the treated larvae survived and developed to pupae, therefore only the minimum irradiation dose of 30 Gy allowed the pupae to develop into adults.

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

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