NEAR-INFRARED FLUORESCENT PROBES FOR SENSITIVE DETERMINATION OF LYSOSOMAL & MITOCHONDRIAL pH IN LIVE CELLS

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols’ concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

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Turn-on visible and ratiometric near-infrared fluorescent probes for distinction endogenous esterases and chymotrypsins in live cells

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2019.127567 ◽

2020 ◽

Vol 306 ◽

pp. 127567 ◽

Cited By ~ 4

Author(s):

Jiemin Wang ◽

Zhidong Teng ◽

Ting Cao ◽

Jing Qian ◽

Lei Zheng ◽

...

Keyword(s):

Fluorescent Probes ◽

Near Infrared ◽

Live Cells ◽

Turn On

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Carbon dots based turn-on fluorescent probes for the sensitive determination of glyphosate in environmental water samples

RSC Advances ◽

10.1039/c6ra10115a ◽

2016 ◽

Vol 6 (89) ◽

pp. 85820-85828 ◽

Cited By ~ 17

Author(s):

Long Wang ◽

Yidan Bi ◽

Jia Gao ◽

Yijia Li ◽

Hong Ding ◽

...

Keyword(s):

Fluorescent Probes ◽

Water Samples ◽

Carbon Dots ◽

Environmental Water ◽

Environmental Water Samples ◽

Turn On ◽

Sensitive Determination

Schematic illustration of Cu2+ and glyphosate detection using the CDs.

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Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes

Molecules ◽

10.3390/molecules24081592 ◽

2019 ◽

Vol 24 (8) ◽

pp. 1592 ◽

Cited By ~ 10

Author(s):

Fang ◽

Xia ◽

Bi ◽

Wigstrom ◽

Valenzano ◽

...

Keyword(s):

Detection Limit ◽

Oxygen Atom ◽

Membrane Permeability ◽

Fluorescent Probes ◽

Near Infrared ◽

Emission Spectra ◽

Live Cells ◽

Phenolic Oxygen ◽

Acetyl Group ◽

Low Cytotoxicity

Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine.

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