Assessment the Activity of Some Enzymes and Antibiotic Substances Sensitivity on Pathogenic Bacteria Species

2018 ◽  
Vol 68 (12) ◽  
pp. 3015-3021 ◽  
Author(s):  
Ionela Sarbu ◽  
Tatiana Vassu ◽  
Mariana Carmen Chifiriuc ◽  
Marcela Bucur ◽  
Ileana Stoica ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Bacterial Vaginosis ◽  
Urinary Tract Infections ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
High Ability ◽  
Opportunistic Pathogens ◽  
Microbial Strains ◽  
Phenotypic Methods ◽  
Tract Infections

Microbial and opportunistic pathogens cause serious problem to human host by releasing different compounds which are involved in colonization, invasion or in alteration of immune process. In this study we propose ourselves to analyze the virulence profile of microorganisms involved in urinary tract infections and bacterial vaginosis in order to compare the virulence mechanisms developed by these microorganisms. We assessed the virulence profile (motility, adhesion to HeLa cell line, biofilm formation, production of enzymes, antibiotic susceptibility) using phenotypic methods. The microbial strains isolated from urinary tract infections present a high ability to release amylases, caseinase, siderophore-like, half of them have present high motility and also were highly resistant to antibiotics, while the microbial strains isolated from bacterial vaginosis present a high ability to bind human epithelial cells and to release hemolyzin and DN-ase.

scholarly journals Exploring the possibilities of infrared spectroscopy for urine sediment examination and detection of pathogenic bacteria in urinary tract infections

2020 ◽  
Vol 58 (10) ◽  
pp. 1759-1767
Author(s):  
Mieke Steenbeke ◽  
Sander De Bruyne ◽  
Jerina Boelens ◽  
Matthijs Oyaert ◽  
Griet Glorieux ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Agar Plate ◽  
Principal Component ◽  
Blood Agar Plate ◽  
Urine Sediment ◽  
Flight Mass Spectrometry ◽  
Tract Infections

AbstractObjectivesIn this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated.MethodsDried urine specimens of control subjects and patients presenting with various nephrological and urological conditions were analysed using mid-infrared spectroscopy (4,000–400 cm−1). Urine samples from patients with a UTI were inoculated on a blood agar plate. After drying of the pure bacterial colonies, FTIR was applied and compared with the results obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemometric data analysis was used to classify the different species.ResultsDue to the typical molecular assignments of lipids, proteins, nucleic acids and carbohydrates, FTIR was able to identify bacteria and showed promising results in the detection of proteins, lipids, white and red blood cells, as well as in the identification of crystals. Principal component analysis (PCA) allowed to differentiate between Gram-negative and Gram-positive species and soft independent modelling of class analogy (SIMCA) revealed promising classification ratios between the different pathogens.ConclusionsFTIR can be considered as a supplementary method for urine sediment examination and for detection of pathogenic bacteria in UTI.

Urinary tract infections in febrile children: Changing spectra of pathogenic bacteria and antibiotic susceptibilities?

2018 ◽  
Vol 55 (6) ◽  
pp. 680-689
Author(s):  
Jus Rakhra ◽  
Gabrielle Williams ◽  
Ben J Marais ◽  
Jonathan C Craig ◽  
Hasantha Gunasekera
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Antibiotic Susceptibilities ◽  
Tract Infections

scholarly journals Vitamin C in the Presence of Sub-Inhibitory Concentration of Aminoglycosides and Fluoroquinolones Alters Proteus mirabilis Biofilm Inhibitory Rate

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 116 ◽  
Author(s):  
Joanna Kwiecińska-Piróg ◽  
Krzysztof Skowron ◽  
Tomasz Bogiel ◽  
Agata Białucha ◽  
Jana Przekwas ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Urinary Tract ◽  
Vitamin C ◽  
Urinary Tract Infections ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Common Species ◽  
Inhibitory Effect ◽  
Acid Addition ◽  
Tract Infections

Vitamin C has antimicrobial activity and is often used as an oral supplement accompanying antibiotic treatment in urinary tract infections (UTI). Proteus mirabilis is the third common species responsible for UTIs that are mostly treated with fluoroquinolones or aminoglycosides. Treatment of the UTI caused by P. mirabilis is problematic due to the ability to form biofilm on the urinary catheters. The aim of the study was to evaluate the influence of ascorbic acid in combination with antibiotics on P. mirabilis abilities to form biofilm. The susceptibility of P. mirabilis reference strain ATCC® 29906™ and four clinical strains isolated from the urine samples of patients with urinary catheter were evaluated according to EUCAST recommendations. The influence of ascorbic acid (0.4 mg × mL−1) in combination with antibiotics on biofilm formation was evaluated spectrophotometrically. Aminoglycosides at sub-inhibitory concentrations more successfully limited biofilm formation by P. mirabilis strains without ascorbic acid addition. Inhibition rate differences at the lowest concentrations of gentamicin and amikacin were statistically significant (p ≤ 0.05). Ascorbic acid addition to the culture medium limited the inhibitory effect of fluoroquinolones, facilitating biofilm formation by P. mirabilis strains. The addition of ascorbic acid during aminoglycosides therapy may disturb treatment of urinary tract infections related to the presence of P. mirabilis biofilm.

scholarly journals Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters

2017 ◽  
Vol 5 (40) ◽  
pp. 8133-8142 ◽  
Author(s):  
Fatima Halouane ◽  
Roxana Jijie ◽  
Dalila Meziane ◽  
Chengnan Li ◽  
Santosh K. Singh ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Selective Isolation ◽  
E Coli ◽  
Reduced Graphene ◽  
Tract Infections ◽  
Efficient Elimination ◽  
Magnetic Reduced Graphene Oxide

The fast and efficient elimination of pathogenic bacteria from water, food or biological samples such as blood remains a challenging task.

scholarly journals Characteristics ofEscherichia colicausing persistence or relapse of urinary tract infections: Phylogenetic groups, virulence factors and biofilm formation

Virulence ◽  
2011 ◽  
Vol 2 (6) ◽  
pp. 528-537 ◽  
Author(s):  
Karen Ejrnæs ◽  
Marc Stegger ◽  
Andreas Reisner ◽  
Sven Ferry ◽  
Tor Monsen ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Virulence Factors ◽  
Urinary Tract Infections ◽  
Biofilm Formation ◽  
Phylogenetic Groups ◽  
Tract Infections

scholarly journals Chemical profiling and antioxidant activity of Equisetum ramosissimum Desf. stem extract, a potential traditional medicinal plant for urinary tract infections

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jeyalatchagan Sureshkumar ◽  
Singamoorthy Amalraj ◽  
Ramar Murugan ◽  
Anantharaj Tamilselvan ◽  
Jayaraj Krupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Urinary Tract ◽  
Medicinal Plant ◽  
Petroleum Ether ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Chemical Profiling ◽  
Stem Extract ◽  
Tract Infections

Abstract Background Equisetum ramosissimum Desf. (E. ramosissimum) is a widely used traditional medicinal plant to treat urinary tract infections (UTIs) by ethnic people throughout the world. The utility of the plant in treating urinary-related disorders was evaluated against selected pathogenic bacteria which has major role in causing UTIs. Hence, the present study executed to extract phytochemicals like total phenolics and flavonoids, chemical profiling by GC–MS analysis and to test their antioxidant activity from stem extracts of E. ramosissimum. The extraction process was directed by petroleum ether, chloroform, ethyl acetate, methanol, and aqueous solvents. Results The GC–MS analysis yielded 24 phytoconstituents with linoleic acid, palmitic acid, nonacosane, hexahydrofarnesyl acetone, and octacosane as major compounds. Methanolic extract yielded maximum amount of phenolics (TPC) and flavonoids (TFC) with 600.02 ± 0.22 mg GAE/g and 631.38 ± 0.69 mg QE/g, respectively. Methanolic extract also exhibited notable free radical scavenging activity with an IC50 of 123.89 ± 0.73, 150.10 ± 1.02, 146.01 ± 0.54, and 63.73 ± 6.12 µg/mL for DPPH, FRAP, ABTS, and O2− assays, respectively. The minimum inhibitory concentration (MIC) required to inhibit the growth of tested pathogenic bacteria was observed in aqueous and methanolic extracts with the value being 31.25 µg/mL against R. equi and V. cholerae. As like, methanolic and petroleum ether extracts efficiently inhibited the growth of B. subtilis with the MIC of 31.25 µg/mL. Conclusion It was concluded that the notable effect of methanolic and aqueous extracts against the uropathogenic bacteria reported in this study supported the traditional uses of this plant in treating UTIs. The results acquired from this investigation revealed that E. ramosissimum stem extract might be considered as an interesting candidate in the development of antibacterial agent against UTIs coupled with antioxidant properties.

scholarly journals Isolation and Identification of Microbes on Hands and Mobile Phones Causing Urinary Tract Infections

2021 ◽  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Mobile Phones ◽  
Pathogenic Bacteria ◽  
University Hospital ◽  
Isolation And Identification ◽  
Civil Hospital ◽  
Transmission Cycle ◽  
Tract Infections

Background: Hands and cell phones are the major source of cross-transmission of urinary tract infections. The aim of this study was to isolate, identify and evaluate Gram-negative bacteria from hand and mobile phones. Methods: This study was conducted in visiting area of Civil Hospital Karachi, Pakistan. Analysis was done by 100 wet sterile cotton tipped swabs, 50 each from mobile phones and hands of their owners. Samples were transported in a Cary Blair transport media, Swabs were streaked on Nutrient agar, Blood agar and MacConkey agar. Organisms were identified by cultural, biochemical, and microscopic characteristics. Results: Total samples n=100 was collected from hand and mobile phones samples (50 hand and 50 mobile swabs) from the Dow university Hospital and Civil Hospital Karachi were tested. Six species of bacteria were isolated along with their identification during the research study. The isolated bacteria were Serratia, Klebsiella, Pseudomonas, Proteus, Shigella and Escherichia coli. The participants’ hands showed high bacterial contamination (50%-56%) in comparison to mobile phones. The frequency (%) of bacteria isolated from mobile phone and hand swabs included Serratia, 12 (24%) with the highest quantity and frequently found bacteria. While, the rest of the results reported Escherichia coli 10 (20%), Klebsiella 9 (18%), Pseudomonas 5 (10%), Shigella 4 (8%) and Proteus 10 (20%) respectively. Conclusion: Patient attendants in hospitals and visitors are more susceptible to nosocomial infections through exchange of mobile phones n=12(24%). Therefore, hygienic practice of hands cleaning while mobile using may help to break the transmission cycle of pathogenic bacteria.

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