Anatomical  and Immunohistochemical Evaluation of Colorectal Cancer

Colorectal cancer is a malignant disease with high morbidity and mortality. Ki-67 is a marker of cell proliferation associated with a low survival in colorectal cancer patients. Its expression is increased in patients with Dukes C or D colorectal cancer versus those in stage A or B. The purpose of the study is to evaluate the immunohistochemical expression of the Ki-67 marker in colorectal adenocarcinomas. The study was performed on a group of 28 patients diagnosed with rectal cancer. We made the histopathological and immunohistochemical evaluation of patients in the study group. The evaluation was performed on the sections stained with hematoxylin-eosin, and for mucinous forms, on sections stained with alcian blue. The topography of the Ki-67 reaction is strictly nuclear, evident in areas with intense proliferative activity. In cases with a strong positive response to Ki-67, the prognosis is worse, with invasion of the perirectal tissues and metastases. The lack of Ki-67 expression in nearby neoplastic tissues suggests that cancerous tissue proliferates in a pathway that it is not correlated with adjacent tissues. The Ki-67 could be useful in the prognosis of patients with colorectal cancer.

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Correlation between IL-10 as Cancer Biomarker and Demographic Characteristics of Colorectal Cancer Patients

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i26b31477 ◽

2021 ◽

pp. 1-10

Author(s):

Rahin Sh Hamad ◽

Bushra H. Shnawa ◽

Shereen J. Al-Ali

Keyword(s):

Colorectal Cancer ◽

Pearson Correlation ◽

Serum Levels ◽

Interleukin 10 ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

High Morbidity ◽

Serum Samples ◽

Cancer Pathogenesis ◽

Colorectal Cancer Patients

Colorectal cancer (CRC) is classified as one of the most prevalent cancer types worldwide, with high morbidity and mortality rates. Patients of CRC have been shown to express a detectable cytokine in serum which contributes to cancer pathogenesis. Therefore, the serum interleukin 10 (IL-10) level in CRC patients was investigated in this study. Patients' medical records with CRC admitted to the Rizgary and Nanakali hospitals, Erbil, Iraq was analyzed as the study group compared to the healthy volunteers' control group. Seventy-one serum samples were collected, thirty-one from diagnosed CRC patients and forty from healthy controls. The concentrations of IL-10 in the sera were assessed by enzyme-linked immunosorbent assay (ELISA). The present finding showed that IL-10 Was significantly elevated in CRC patients' sera compared to the control group, suggesting confirmation of its usefulness for detecting CRC patients' prognosis. A non-significant Pearson correlation was detected between IL-10 serum levels and the CRC group's age, gender, and body mass index. Herein is the first study on the evaluation of IL-10 levels in CRC patients in Kurdistan, Iraq.

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Clinical–pathological characteristics and short-term follow-up associated with proliferation, apoptosis and angiogenesis in a prospective cohort of patients with colorectal tumours

Tumor Biology ◽

10.1177/1010428319835684 ◽

2019 ◽

Vol 41 (4) ◽

pp. 101042831983568

Author(s):

Maximino Redondo ◽

Cristina Abitei ◽

Teresa Téllez ◽

Rafael Fúnez ◽

Teresa Pereda ◽

...

Keyword(s):

Colorectal Cancer ◽

Prospective Cohort ◽

Good Prognosis ◽

Terminal Deoxynucleotidyl Transferase ◽

Short Term ◽

Ki 67 ◽

Term Survival ◽

Colorectal Tumour ◽

Colorectal Cancer Patients

We investigate the clinical and pathological features related to variations in colorectal tumour apoptosis, proliferation and angiogenesis and the influence of the latter in short-term mortality (2 years); 551 tumour samples from a prospective cohort of patients with colorectal cancer were examined and tumour biology markers were determined as follows: percentage of apoptotic cells, by the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling technique; Ki-67 antigen, as a cell proliferation marker and density of microvessels (as a marker of angiogenesis). An increase in the percentage of cellular apoptosis is significantly related to the presence of poorly differentiated tumours, with vascular invasion (p < 0.001). The CD105 angiogenesis marker is not related to any clinical–pathological parameter except that of higher frequency in older patients (p = 0.03). Ki-67 is more frequently expressed in tumours with less nervous invasion (p = 0.05). Neither apoptosis nor angiogenesis present any significant association with short-term survival. The only marker clearly related to 2-year survival is Ki-67, which is shown to be a good prognostic factor in the multivariate analysis (hazard ratio = 0.49; 95% confidence interval = 0.27–0.90). Therefore, in a prospective cohort of colorectal cancer patients, only Ki-67 is a marker of good prognosis in short-term follow-up.

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The prognostic role of tissue TLR2 and TLR4 in colorectal cancer

Virchows Archiv ◽

10.1007/s00428-020-02833-5 ◽

2020 ◽

Vol 477 (5) ◽

pp. 705-715

Author(s):

Ines Beilmann-Lehtonen ◽

Camilla Böckelman ◽

Harri Mustonen ◽

Selja Koskensalo ◽

Jaana Hagström ◽

...

Keyword(s):

Colorectal Cancer ◽

Prognostic Factor ◽

Distant Metastases ◽

University Hospital ◽

Rank Test ◽

Pathogen Invasion ◽

Kaplan Meier ◽

Node Positive Disease ◽

Colorectal Cancer Patients ◽

Dukes C

Abstract Colorectal cancer (CRC), the second most common cancer globally, resulted in 881,000 deaths in 2018. Toll-like receptors (TLRs) are crucial to detecting pathogen invasion and inducing the host’s immune response. This study aimed to explore the prognostic value of TLR2 and TLR4 tumor expressions in colorectal cancer patients. We studied the immunohistochemical expressions of TLR2 and TLR4 using tissue microarray specimens from 825 patients undergoing surgery in the Department of Surgery, Helsinki University Hospital, between 1982 and 2002. We assessed the relationships between TLR2 and TLR4 expressions and clinicopathological variables and patient survival. We generated survival curves using the Kaplan-Meier method, determining significance with the log-rank test. Among patients with lymph node–positive disease and no distant metastases (Dukes C), a strong TLR2 immunoactivity associated with a better prognosis (p < 0.001). Among patients with local Dukes B disease, a strong TLR4 immunoactivity associated with a worse disease-specific survival (DSS; p = 0.017). In the multivariate survival analysis, moderate TLR4 immunoactivity compared with strong TLR4 immunoactivity (hazard ratio (HR) 0.66, 95% confidence interval (CI) 0.49–0.89, p = 0.007) served as an independent prognostic factor. In the multivariate analysis for the Dukes subgroups, moderate TLR2 immunoactivity (HR 2.63, 95% CI 1.56–4.44, p < 0.001) compared with strong TLR2 immunoactivity served as an independent negative prognostic factor in the Dukes C subgroup. TLR2 and TLR4 might be new prognostic factors to indicate which CRC patients require adjuvant therapy and which could spare from an unnecessary follow-up, but further investigations are needed.

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Immunohistochemical expression of Notch1, Jagged1, NF-κB and MMP-9 in colorectal cancer patients and the relationship to clinicopathological parameters

Cancer Biomarkers ◽

10.3233/cbm-150533 ◽

2015 ◽

Vol 15 (6) ◽

pp. 889-897 ◽

Cited By ~ 14

Author(s):

Chen-Guo Zheng ◽

Rong Chen ◽

Jie-Bin Xie ◽

Chang-Bao Liu ◽

Zhao Jin ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Clinicopathological Parameters ◽

Immunohistochemical Expression ◽

Colorectal Cancer Patients ◽

The Relationship

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Prognostic valude of Ki-67, TS, p53, bcl-2, EGFR, MLH1 and MSH2 in 107 consecutive colorectal cancer patients

Journal of Clinical Oncology ◽

10.1200/jco.2005.23.16_suppl.3694 ◽

2005 ◽

Vol 23 (16_suppl) ◽

pp. 3694-3694

Author(s):

C. Pinto ◽

C. Ceccarelli ◽

C. Funaioli ◽

V. Mutri ◽

F. L. Rojas Llimpe ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Ki 67 ◽

Colorectal Cancer Patients

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ErbB family immunohistochemical expression in colorectal cancer patients with higher risk of recurrence after radical surgery

International Journal of Colorectal Disease ◽

10.1007/s00384-009-0702-6 ◽

2009 ◽

Vol 24 (9) ◽

pp. 1059-1068 ◽

Cited By ~ 30

Author(s):

Glauco Baiocchi ◽

Ademar Lopes ◽

Renata A. Coudry ◽

Benedito M. Rossi ◽

Fernando A. Soares ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Radical Surgery ◽

Immunohistochemical Expression ◽

Risk Of Recurrence ◽

Erbb Family ◽

Colorectal Cancer Patients

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Mismatch Repair Proteins and Microsatellite Instability in Colorectal Carcinoma (MLH1, MSH2, MSH6 and PMS2): Histopathological and Immunohistochemical Study

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.003 ◽

2017 ◽

Vol 5 (1) ◽

pp. 9-13 ◽

Cited By ~ 5

Author(s):

Nour El Hoda S. Ismael ◽

Samar A. El Sheikh ◽

Suzan M. Talaat ◽

Eman M. Salem

Keyword(s):

Colorectal Cancer ◽

Microsatellite Instability ◽

Mismatch Repair ◽

Immunohistochemical Study ◽

Survival Rates ◽

Immunohistochemical Expression ◽

Egyptian Patients ◽

Mismatch Repair Proteins ◽

Microsatellite Stability ◽

Colorectal Cancer Patients

BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers worldwide. Microsatellite instability (MSI) is detected in about 15% of all colorectal cancers. CRC with MSI has particular characteristics such as improved survival rates and better prognosis. They also have a distinct sensitivity to the action of chemotherapy.AIM: The aim of the study was to detect microsatellite instability in a cohort of colorectal cancer Egyptian patients using the immunohistochemical expression of mismatch repair proteins (MLH1, MSH2, MSH6 and PMS2).MATERIAL AND METHODS: Cases were divided into Microsatellite stable (MSS), Microsatellite unstable low (MSI-L) and Microsatellite unstable high (MSI-H). This Microsatellite stability status was correlated with different clinicopathological parameters.RESULTS: There was a statistically significant correlation between the age of cases, tumor site & grade and the microsatellite stability status. There was no statistically significant correlation between the gender of patients, tumor subtype, stage, mucoid change, necrosis, tumor borders, lymphocytic response, lymphovascular emboli and the microsatellite stability status.CONCLUSION: Testing for MSI should be done for all colorectal cancer patients, especially those younger than 50 years old, right sided and high-grade CRCs.

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hERG1 and HIF-2α Behave as Biomarkers of Positive Response to Bevacizumab in Metastatic Colorectal Cancer Patients

Translational Oncology ◽

10.1016/j.tranon.2020.01.001 ◽

2020 ◽

Vol 13 (3) ◽

pp. 100740 ◽

Cited By ~ 5

Author(s):

Jessica Iorio ◽

Elena Lastraioli ◽

Lorenzo Tofani ◽

Giulia Petroni ◽

Lorenzo Antonuzzo ◽

...

Keyword(s):

Colorectal Cancer ◽

Metastatic Colorectal Cancer ◽

Cancer Patients ◽

Positive Response ◽

Colorectal Cancer Patients

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The Effect of Anesthesia on the Immune System in Colorectal Cancer Patients

Canadian Journal of Gastroenterology and Hepatology ◽

10.1155/2018/7940603 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Yangjie Dang ◽

Xingxing Shi ◽

William Xu ◽

Mingzhang Zuo

Keyword(s):

Colorectal Cancer ◽

Immune System ◽

Stress Response ◽

Cancer Patients ◽

Surgical Excision ◽

Perioperative Period ◽

Postoperative Recurrence ◽

High Morbidity ◽

Cancer Dissemination ◽

Colorectal Cancer Patients

Colorectal cancer (CRC) is the key leading cause of high morbidity and mortality worldwide. Surgical excision is the most effective treatment for CRC. However, stress caused by surgery response can destroy the body’s immunity and increase the likelihood of cancer dissemination and metastasis. Anesthesia is an effective way to control the stress response, and recent basic and clinical research has shown that anesthesia and related drugs can directly or indirectly affect the immune system of colorectal cancer patients during the perioperative period. Thus, these drugs may affect the prognosis of CRC surgery patients. This review is intended to summarize currently available data regarding the effects of anesthetics and related drugs on perioperative immune function and postoperative recurrence and metastasis in CRC patients. Determining the most suitable anesthesia for patients with CRC is of utmost importance.

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