scholarly journals Chemical, Antimicrobial, Antioxidant and Anti-proliferative Features of the Essential Oil Extracted from the Invasive Plant Solidago canadensis L.

2020 ◽  
Vol 71 (7) ◽  
pp. 255-264
Author(s):  
Ioana-Cristina Marinas ◽  
Eliza Oprea ◽  
Mihaela Buleandra ◽  
Coralia Bleotu ◽  
Irinel Adriana Badea ◽  
...  
Keyword(s):  
Essential Oil ◽  
Invasive Plant ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Intracellular Ros ◽  
Adherence Pattern ◽  
Microbial Strains ◽  
Bacteria And Fungi ◽  
Main Components

The essential oil from inflorescences of S. canadensis L. (Goldenrod) obtained by hidrodistillation was analysed by gas chromatography-mass spectrometry and it was qualitatively and quantitatively tested against Gram-positive and Gram-negative bacteria and fungi. The samples were also subjected to screening for their possible antioxidant activity by using DPPH assay and the influence on intracellular ROS (reactive oxygen species). The main components identified were a-pinene, germacrene D, and limonene. The tested microbial strains proved to be susceptible to S. canadensis essential oil which exhibited good anti-biofilm activity, inhibiting the adhesion to the inert and cellular substrate, decreasing the Acinetobacter baumannii adhesion index to 17.52% and changing the adherence pattern. Goldenrod essential oil showed good free-radical scavenging activity, but it increased the production of free radicals in Hep-2 tumor cells.

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

scholarly journals Chemical Composition and Biological Activities of Oregano Essential Oil and Its Fractions Obtained by Vacuum Distillation

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1904 ◽  
Author(s):  
Magdalena de J. Rostro-Alanis ◽  
Juan Báez-González ◽  
Cynthia Torres-Alvarez ◽  
Roberto Parra-Saldívar ◽  
José Rodriguez-Rodriguez ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Vacuum Distillation ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Oregano Essential Oil ◽  
Almost All

Oregano (Poliomintha longiflora) essential oil (Ooil) is a product of high commercial value and many applications, including chemotherapy. Aiming to achieve the best use of this resource, the present study focuses on the characterization of separated fractions of Ooil by fractional vacuum distillation at low pressure. Four fractions (F1–F4) and undistilled oil (Unoil) were separated from Ooil and analyzed for their chemical composition and biological activities, such as antioxidant and antimicrobial activities. Gas chromatography–mass spectrometry shows differences in the composition among the fractions and Ooil. The amount of monoterpenes oxygenated (MO), sesquiterpenes hydrocarbon (SeH) and monoterpenes hydrocarbon (MH) varied between the fractions in ranges of 1.51–68.08, 3.31–25.12 and 1.91–97.75%, respectively. The major concentrations of MO and SeH were observed in F4 and Unoil. On the other hand, the highest concentrations of MH were found in F1 and F2, while the lowest were in F4 and Unoil. These results were correlated with the biological activity. Free-radical scavenging activity varied among fractions, with F4 and Unoil showing the highest activity. The antimicrobial test showed that F4 and Unoil had the highest activity in almost all cases. The correlation between the variables studied in the different fractions allows the definition of the particular properties for each one of them.

scholarly journals Antioxidant and Antimicrobial Properties of the Essential Oil and Extracts ofZanthoxylum alatumGrown in North-Western Himalaya

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Sanjay Guleria ◽  
A. K. Tiku ◽  
Apurva Koul ◽  
Sahil Gupta ◽  
Gurjinder Singh ◽  
...  
Keyword(s):  
Essential Oil ◽  
Methanolic Extract ◽  
Micrococcus Luteus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Preparative Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Curvularia Lunata

The essential oil obtained from the fresh leaves ofZanthoxylum alatumwas analysed by gas chromatography/mass spectrometry (GC/MS). Fourteen components were identified, and linalool (30.58%), 2-decanone (20.85%),β-fenchol (9.43%), 2-tridecanone (8.86%),β-phellandrene (5.99%), Sabinene (4.82%), andα-pinene (4.11%) were the main components. The EO and methanolic extract ofZ. alatumexhibited potent antifungal activity againstAlternaria alternata,Alternaria brassicae, andCurvularia lunata. The EO also showed significant antibacterial activity againstBacillus subtilis,Micrococcus luteus,Staphylococcus aureus, andEscherichia coli. Further, antimicrobial constituents of the EO were isolated by bioautography and preparative thin layer chromatography (PTLC) and identified asβ-fenchol and linalool using GC/MS analysis. In addition to this, the free radical scavenging activity and antioxidant potential of EO and methanolic extract/fractions ofZ. alatumwere also investigated usingin vitroassays including scavenging ability against DPPH•, reducing power and chelating ability on Fe2+ions. Our results demonstrate thatZ. alatumcould be used as a resource of antioxidant and antimicrobial compounds which may find applications in food and pesticide industries.

scholarly journals Chemical Composition, Antibacterial and Antioxidant Activity of the Essential Oil of Bupleurum longiradiatum

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Baojun Shi ◽  
Wei Liu ◽  
Shao-peng Wei ◽  
Wen-jun Wu
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Bupleurum Longiradiatum

The essential oil from the roots of Bupleurum longiradiatum, obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial activity and antioxidant activity. Fifty-one compounds were identified, representing 99.3% of the total oil. The major constituents were thymol (7.0%), butylidene phthalide (6.8%), 5-indolol (5.6%), heptanal (5.3%), 4-hydroxy-2-methylacetophenone (5.3%), 4,5-diethyl-octane (5.3%), bormeol (5.1%) and hexanoic acid (5.1%). The oil was tested against 4 bacteria at different concentrations using disc diffusion and 96-well dilution methods. The inhibition zones and minimum inhibitory concentration values for bacterial strains were in the range of 7.0–18.0 mm and 250 −500 μg/mL, respectively. The in vitro antioxidant activity was assessed by DPPH radical scavenging and inhibition of lipid peroxidation methods. The oil showed a potent free radical scavenging activity, as evidenced by the low IC50 value for DPPH radical (566.2μg/mL) and inhibition of lipid peroxidation (induced by FeSO4, H2O2 and CCl4) with IC50 values of 275.2 μg/mL, 296.9 μg/mL and 118.7 μg/mL, respectively.

scholarly journals Composition and Antioxidant Activity of Essential Oils of Lippia Origanoides H.B.K. grown in Colombia

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Elena Stashenko ◽  
Carlos Ruiz ◽  
Amner Muñoz ◽  
Martha Castañeda ◽  
Jairo Martínez
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
The Other ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Compound Identification ◽  
Microwave Assisted ◽  
Main Components

Microwave-assisted hydrodistillation, simultaneous distillation-solvent extraction, and supercritical fluid extraction, were used to isolate secondary metabolites from two Lippia origanoides chemotypes growing wild in Colombia. Compound identification was based on chromatographic and spectroscopic criteria. The main components identified in the essential oil of one chemotype were carvacrol (44.4 – 51.8%) and p-cymene (8.8 – 10.1%). Those of the other chemotype were p-cymene (11.3 – 15.7%) and 1,8-cineole (6.8 – 10.9%). The last one constitutes a new chemotype, characterized by its low thymol and carvacrol contents and very low antioxidant activity. The radical scavenging activity of the essential oil of the first chemotype was higher than that of butylated hydroxytoluene (BHT) and similar to that of α-tocopherol.

Effects of Thymus revolutus Célak essential oil and its two major components on Hep G2 cells membrane

Biologia ◽  
2013 ◽  
Vol 68 (1) ◽  
Author(s):  
Ayse Erdogan ◽  
Aysun Ozkan
Keyword(s):  
Linoleic Acid ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Membrane Damage ◽  
Radical Scavenging ◽  
Acid Oxidation ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Main Components

AbstractIn this study pro/anti-oxidant activities of Thymus revolutus Célak (T. revolutus C.) essential oil and its two main components on Hep G2 cells were determined. Twelve components were identified in T. revolutus C. essential oil by GC and GC-MS and the main components of the oil were cymene (32.57%) and γ-terpinene (17.18%). The essential oil showed more cytotoxicity than its two main components on Hep G2 after 24, 48 and 72 hours incubations. Membrane damage effects of the essential oil and its two main components on Hep G2 cells were increased by incubation with IC10, IC50 and IC70 concentrations. Lower essential oil (<IC50) concentrations had cytoprotective and membrane protective effects against H2O2. The free radical scavenging activity of the essential oil of T. revolutus C. (EC50 = 250±0.2 μg/mL) was superior to its two main components (neither not active). Oxidation of linoleic acid was effectively inhibited by T. revolutus C. (72.8%), while inhibition values of linoleic acid oxidation were calculated as 23.4.% and 26.7% for cymene and Γ-terpinene, respectively. These findings suggest that essential oils and its two main components had both pro-oxidant and protective (antioxidant) effects and they exhibited those effects depending on concentration.

scholarly journals Chemical composition and biological activity of Nepeta cilicica

2017 ◽  
Vol 12 (2) ◽  
pp. 20 ◽  
Author(s):  
Gökalp İşcan ◽  
Fatih Göger ◽  
Betül Demirci ◽  
Yavuz Bülent Köse
Keyword(s):  
Essential Oil ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Video Clip ◽  
Radical Scavenging ◽  
Inhibitory Effects ◽  
Plant Materials ◽  
Aerial Parts ◽  
Full Screen ◽  
Main Components

<p>In the present study, aerial parts of <em>Nepeta cilicica</em> were hydro-distilled for the essential oil. Furthermore, air-dried plant materials were extracted with methanol. According to GC-MS results, 35 compounds were characterized in the oil (totally 92%). Caryophyllene oxide, β-caryophyllene and spathulenol (28.2, 8.9 and 4.2%) were found as main components of the oil. Sixteen compounds were detected by LC/MS, most of which were caffeoylquinic acid, luteolin and apigenin derivatives, in methanolic extract of the N. cilicica. Apart from DPPH radical scavenging activity, inhibitory effects on bacterial and yeast growth of essential oil and the methanolic extract were evaluated. Twenty-four different pathogenic bacterial and <em>Candida</em> strains were tested by M7-A7 and CLSI M27-A2 protocols respectively. The crude extract showed better inhibitory effects against bacteria strains than the essential oil. Both oil and the extract demonstrated strong inhibition on <em>C. tropicalis</em> at a concentration of 47.0 µg/mL (MIC).</p><p><strong>Video Clip of Methodology</strong>:</p><p>CLSI microdilution broth method: 3 min 46 sec   <a href="https://youtube.com/v/PBADMG2zG1c">Full Screen</a>   <a href="https://youtube.com/watch?v=PBADMG2zG1c">Alternate</a></p>

scholarly journals Volatiles Profiling, Allelopathic Activity, and Antioxidant Potentiality of Xanthium Strumarium Leaves Essential Oil from Egypt: Evidence from Chemometrics Analysis

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 584 ◽  
Author(s):  
Ahmed El-Gawad ◽  
Abdelsamed Elshamy ◽  
Abd El Gendy ◽  
Ahmed Gaara ◽  
Abdulaziz Assaeed
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Xanthium Strumarium ◽  
Bornyl Acetate ◽  
Dependent Manner ◽  
Bidens Pilosa ◽  
Concentration Dependent Manner ◽  
Allelopathic Potential

The essential oil (EO) of Xanthium strumarium L. leaves (family: Asteraceae) was extracted by hydrodistillation, and then analyzed by gas chromatography-mass spectrometry (GC-MS). Forty-three essential compounds were identified. The sesquiterpenoids represented the major constituents (72.4%), including oxygenated (61.78%) and non-oxygenated (10.62%) sesquiterpenes, followed by monoterpenes (25.19%). The diterpenoids and oxygenated hydrocarbons were determined as minor compounds. The main constituents of the EO were 1,5-dimethyltetralin (14.27%), eudesmol (10.60%), l-borneol (6.59%), ledene alcohol (6.46%), (-)-caryophyllene oxide (5.36%), isolongifolene, 7,8-dehydro-8a-hydroxy (5.06%), L-bornyl acetate (3.77%), and aristolene epoxide (3.58%). A comparative analysis was stated here between the EO of Egyptian X. strumarium and those previously reported from Pakistan, Iran, and Brazil based on chemometic tools such as principal components analysis (PCA) and agglomerative hierarchical clustering (AHC). The EO of X. strumarium showed weak 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity with IC50 321.93 µL/L−1, which was comparable to ascorbic acid as a reference. However, the EO exhibited significant allelopathic potential regarding the germination and growth of the noxious weed Bidens pilosa in a concentration-dependent manner. Therefore, further study is recommended to characterize the EO from X. strumarium as an eco-friendly green bioherbicide against weeds, as well as determine their mode of actions.

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) and evaluation of antioxidant and antimicrobial activities of essential oil of Campomanesia adamantium (Cambess.) O. Berg (Guavira)

2009 ◽  
Vol 45 (4) ◽  
pp. 767-776 ◽  
Author(s):  
Isabel Duarte Coutinho ◽  
Claúdia Andréa Lima Cardoso ◽  
Nilva Ré-Poppi ◽  
Adriana Mestriner Melo ◽  
Maria do Carmo Vieira ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reproductive Stage ◽  
Gas Chromatography Mass Spectrometry ◽  
Moderate Activity ◽  
Chromatography Mass Spectrometry

The essential oils from Campomanesia adamantium (Cambess.) O. Berg leaves, collected in the reproductive (flowering and fruit-bearing) and vegetative stages, were characterized by GC-MS (Gas Chromatography-Mass Spectrometry). A total of 95 compounds of the essential oils were identified. In the reproductive stage (flowering) the major constituents were monoterpenes (limonene, α-pinene and β-pinene) while during the vegetative stage the major constituents were the sesquiterpenes (bicyclogermacrene and globulol). The essential oil of the reproductive stage shows high antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, and all show moderate activity against Escherichia coli. The essential oils were also evaluated for their radical-scavenging activity by DPPH. The chemogeographical variations of the oil composition from the four distinct localities studied all contained α-pinene, β-pinene, limonene, linalool, β-caryophyllene, germacrene D and bicyclogermacrene, however the samples from Jardim city contained neither limonene nor linalool.

scholarly journals Composition, Cytotoxicity and Antioxidant Activity of the Essential Oil of Dracocephalum surmandinum from Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Ali Esmaeili ◽  
Abbas Gholipour ◽  
Mohammad Reza Kanani
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leukemia Cell ◽  
Model Systems ◽  
Good Effect ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Cytotoxicity Activity ◽  
Main Components

The composition of the hydrodistilled essential oil from the aerial parts of Dracocephalum surmandinum was analyzed by GC and GC-MS. Monoterpenoids, including oxygenated compounds and hydrocarbons, comprising 63.4 and 33.9%, respectively, were the principal compound groups of the essential oil. In total, 25 constituents accounting for 97.8% of the oil were identified. Perilla aldehyde (54.3%) and limonene (30.1%) were characterized as the main components. The essential oil of D. surmandinum and its two main constituents showed a marked antioxidant and radical scavenging activity using different model systems, including 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and β-carotene-linoleic acid blenching assays. The results of cytotoxicity activity using the MTT assay exhibited that the oil of D. surmandinum has a good effect against human breast adenocarcinoma (MCF-7) and erythromyeloblastoid leukemia cell lines (K562) with IC50 values of 14 and 16 μg/mL, respectively. The cytotoxic potential of D. surmandinum oil against rat adrenal pheochromocytoma cell line (PC 12) was weak (IC50 of >100 μg/mL). Perilla aldehyde and limonene, the main constituents of D. surmandinum essential oil, inhibited all investigated cell growth in a dose- and time-dependent manner with IC50 values ranged from 0.25-5.0 mmol/L.

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