Effects of Thymus revolutus Célak essential oil and its two major components on Hep G2 cells membrane

Biologia ◽  
2013 ◽  
Vol 68 (1) ◽  
Author(s):  
Ayse Erdogan ◽  
Aysun Ozkan
Keyword(s):  
Linoleic Acid ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Membrane Damage ◽  
Radical Scavenging ◽  
Acid Oxidation ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Main Components

AbstractIn this study pro/anti-oxidant activities of Thymus revolutus Célak (T. revolutus C.) essential oil and its two main components on Hep G2 cells were determined. Twelve components were identified in T. revolutus C. essential oil by GC and GC-MS and the main components of the oil were cymene (32.57%) and γ-terpinene (17.18%). The essential oil showed more cytotoxicity than its two main components on Hep G2 after 24, 48 and 72 hours incubations. Membrane damage effects of the essential oil and its two main components on Hep G2 cells were increased by incubation with IC10, IC50 and IC70 concentrations. Lower essential oil (<IC50) concentrations had cytoprotective and membrane protective effects against H2O2. The free radical scavenging activity of the essential oil of T. revolutus C. (EC50 = 250±0.2 μg/mL) was superior to its two main components (neither not active). Oxidation of linoleic acid was effectively inhibited by T. revolutus C. (72.8%), while inhibition values of linoleic acid oxidation were calculated as 23.4.% and 26.7% for cymene and Γ-terpinene, respectively. These findings suggest that essential oils and its two main components had both pro-oxidant and protective (antioxidant) effects and they exhibited those effects depending on concentration.

scholarly journals Anthocyanin-rich purple potato flake extract has antioxidant capacity and improves antioxidant potential in rats

2006 ◽  
Vol 96 (6) ◽  
pp. 1125-1134 ◽  
Author(s):  
Kyu-Ho Han ◽  
Mitsuo Sekikawa ◽  
Ken-ichiro Shimada ◽  
Makoto Hashimoto ◽  
Naoto Hashimoto ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Group ◽  
Acid Oxidation ◽  
Mrna Levels ◽  
Scavenging Activity ◽  
Potato Flake ◽  
Linoleic Acid Oxidation ◽  
Potato Flakes

Anthocyanins from various vegetables and fruits have antioxidant activities, however, the bioactivities of coloured potato anthocyanins are not well studied. We examined the antioxidant capacities of pigmented fractions from purple potato flakes in vitro, and the antioxidant potentials of purple potato flakes in vivo. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of the pigmented fraction from Hokkai no. 92 (H92) potato flakes was higher than that from Kitamurasaki (KM) potato flakes. Extracts equivalent to 600 μg pigmented fractions from KM and H92 potato flakes inhibited linoleic acid oxidation in the order trolox>H92 ≥ KM>control. Rats were fed 25 % KM or H92 potato flake diets for 4 weeks. The major anthocyanin was identified as petanin. Control rats were fed a diet with cornstarch instead of potato flakes for 4 weeks. The serum antioxidant potential level in the H92 group was significantly higher than that in the control group. The degree of hepatic lipid peroxidation in the H92 group was significantly lower than that in the control group. Hepatic Cu/Zn-superoxide dismutase (SOD), Mn-SOD and glutathione peroxidase (GSH-Px) mRNA levels in the H92 group were significantly higher than those in the control group. Similar significant differences in Cu/Zn-SOD and Mn-SOD mRNA levels between the KM and control groups were found. The present results suggest that purple potato flakes have antioxidant functions with regard to radical scavenging activity and inhibition of linoleic acid oxidation, and that they improve the antioxidant potentials in rats by enhancing hepatic Mn-SOD, Cu/Zn-SOD and GSH-Px mRNA expression.

scholarly journals APOPTOSIS-INDUCING POTENTIAL OF LAWSONIA ALBA LAM. LEAVES ON HEPATOCELLULAR CARCINOMA (HEP-G2) CELLS ALONG WITH ITS ANTI-OXIDANT PROPERTY

2016 ◽  
Vol 8 (9) ◽  
pp. 156
Author(s):  
Soumyasree Dutta ◽  
Nilanjana Deb ◽  
Ashok Kumar Pattnaik ◽  
Shila Elizabeth Besra
Keyword(s):  
Ethyl Acetate ◽  
Flow Cytometric Analysis ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 Cells ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Anti Oxidant ◽  
G2 Cells

<p><strong>Objective: </strong>The current study investigated the anti-cancer potential of methanolic and ethyl acetate fraction of <em>Lawsonia alba</em> L. (Lythraceae) leaves extract on Hep-G2 and RAW 264.7 cells along with <em>in vitro</em> anti-oxidant property of the ethyl acetate fraction.</p><p><strong>Methods: </strong>The cytotoxic activity of methanolic extract and its fractions had been studied by MTT assay on Hep-G2 and RAW 264.7 cells. Morphological study of Hep-G2 cells was performed by light, fluorescence and confocal microscope. 1% agarose gel electrophoresis, detection of apoptosis and cell cycle arrest by flow cytometric analysis had been performed to determine the proportion and stages of cellular apoptosis of Hep-G2 cells. <em>In vitro</em> anti-oxidant study of various fractions of MLA were performed by DPPH and Hydroxyl radical scavenging assay.</p><p><strong>Results: </strong>Cytotoxicity study of MLA and ELA had been confirmed by MTT assay and the IC<sub>50</sub> value were calculated to be 75.85μg/ml and 32.81μg/ml on Hep-G2 cell line respectively. Morphological study showed the arrays of nuclear changes including chromatin condensation and apoptotic body formation indicating that treatment with ELA, causes apoptotic changes in the hepatoma cells compared to the untreated control. Agarose gel electrophoresis study showed fragmented DNA in the form of a ladder. Flow cytometric analysis showed an appreciable number of cells in early apoptosis stage. The cells were arrested, mostly in G0/G1 phase of the cell cycle. Antioxidant property of ELA fraction was confirmed by free radical scavenging activity.</p><p><strong>Conclusion: </strong>Ethyl acetate fraction of <em>Lawsonia alba</em> L. leaves possess potent apoptotic activity against Hep-G2 cell line along with notable anti-oxidant activity.</p>

scholarly journals Composition and Antioxidant Activity of Essential Oils of Lippia Origanoides H.B.K. grown in Colombia

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Elena Stashenko ◽  
Carlos Ruiz ◽  
Amner Muñoz ◽  
Martha Castañeda ◽  
Jairo Martínez
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
The Other ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Compound Identification ◽  
Microwave Assisted ◽  
Main Components

Microwave-assisted hydrodistillation, simultaneous distillation-solvent extraction, and supercritical fluid extraction, were used to isolate secondary metabolites from two Lippia origanoides chemotypes growing wild in Colombia. Compound identification was based on chromatographic and spectroscopic criteria. The main components identified in the essential oil of one chemotype were carvacrol (44.4 – 51.8%) and p-cymene (8.8 – 10.1%). Those of the other chemotype were p-cymene (11.3 – 15.7%) and 1,8-cineole (6.8 – 10.9%). The last one constitutes a new chemotype, characterized by its low thymol and carvacrol contents and very low antioxidant activity. The radical scavenging activity of the essential oil of the first chemotype was higher than that of butylated hydroxytoluene (BHT) and similar to that of α-tocopherol.

scholarly journals Chemical composition and biological activity of Nepeta cilicica

2017 ◽  
Vol 12 (2) ◽  
pp. 20 ◽  
Author(s):  
Gökalp İşcan ◽  
Fatih Göger ◽  
Betül Demirci ◽  
Yavuz Bülent Köse
Keyword(s):  
Essential Oil ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Video Clip ◽  
Radical Scavenging ◽  
Inhibitory Effects ◽  
Plant Materials ◽  
Aerial Parts ◽  
Full Screen ◽  
Main Components

<p>In the present study, aerial parts of <em>Nepeta cilicica</em> were hydro-distilled for the essential oil. Furthermore, air-dried plant materials were extracted with methanol. According to GC-MS results, 35 compounds were characterized in the oil (totally 92%). Caryophyllene oxide, β-caryophyllene and spathulenol (28.2, 8.9 and 4.2%) were found as main components of the oil. Sixteen compounds were detected by LC/MS, most of which were caffeoylquinic acid, luteolin and apigenin derivatives, in methanolic extract of the N. cilicica. Apart from DPPH radical scavenging activity, inhibitory effects on bacterial and yeast growth of essential oil and the methanolic extract were evaluated. Twenty-four different pathogenic bacterial and <em>Candida</em> strains were tested by M7-A7 and CLSI M27-A2 protocols respectively. The crude extract showed better inhibitory effects against bacteria strains than the essential oil. Both oil and the extract demonstrated strong inhibition on <em>C. tropicalis</em> at a concentration of 47.0 µg/mL (MIC).</p><p><strong>Video Clip of Methodology</strong>:</p><p>CLSI microdilution broth method: 3 min 46 sec   <a href="https://youtube.com/v/PBADMG2zG1c">Full Screen</a>   <a href="https://youtube.com/watch?v=PBADMG2zG1c">Alternate</a></p>

scholarly journals Chemical, Antimicrobial, Antioxidant and Anti-proliferative Features of the Essential Oil Extracted from the Invasive Plant Solidago canadensis L.

2020 ◽  
Vol 71 (7) ◽  
pp. 255-264
Author(s):  
Ioana-Cristina Marinas ◽  
Eliza Oprea ◽  
Mihaela Buleandra ◽  
Coralia Bleotu ◽  
Irinel Adriana Badea ◽  
...  
Keyword(s):  
Essential Oil ◽  
Invasive Plant ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Intracellular Ros ◽  
Adherence Pattern ◽  
Microbial Strains ◽  
Bacteria And Fungi ◽  
Main Components

The essential oil from inflorescences of S. canadensis L. (Goldenrod) obtained by hidrodistillation was analysed by gas chromatography-mass spectrometry and it was qualitatively and quantitatively tested against Gram-positive and Gram-negative bacteria and fungi. The samples were also subjected to screening for their possible antioxidant activity by using DPPH assay and the influence on intracellular ROS (reactive oxygen species). The main components identified were a-pinene, germacrene D, and limonene. The tested microbial strains proved to be susceptible to S. canadensis essential oil which exhibited good anti-biofilm activity, inhibiting the adhesion to the inert and cellular substrate, decreasing the Acinetobacter baumannii adhesion index to 17.52% and changing the adherence pattern. Goldenrod essential oil showed good free-radical scavenging activity, but it increased the production of free radicals in Hep-2 tumor cells.

scholarly journals Composition, Cytotoxicity and Antioxidant Activity of the Essential Oil of Dracocephalum surmandinum from Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Ali Esmaeili ◽  
Abbas Gholipour ◽  
Mohammad Reza Kanani
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leukemia Cell ◽  
Model Systems ◽  
Good Effect ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Cytotoxicity Activity ◽  
Main Components

The composition of the hydrodistilled essential oil from the aerial parts of Dracocephalum surmandinum was analyzed by GC and GC-MS. Monoterpenoids, including oxygenated compounds and hydrocarbons, comprising 63.4 and 33.9%, respectively, were the principal compound groups of the essential oil. In total, 25 constituents accounting for 97.8% of the oil were identified. Perilla aldehyde (54.3%) and limonene (30.1%) were characterized as the main components. The essential oil of D. surmandinum and its two main constituents showed a marked antioxidant and radical scavenging activity using different model systems, including 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and β-carotene-linoleic acid blenching assays. The results of cytotoxicity activity using the MTT assay exhibited that the oil of D. surmandinum has a good effect against human breast adenocarcinoma (MCF-7) and erythromyeloblastoid leukemia cell lines (K562) with IC50 values of 14 and 16 μg/mL, respectively. The cytotoxic potential of D. surmandinum oil against rat adrenal pheochromocytoma cell line (PC 12) was weak (IC50 of >100 μg/mL). Perilla aldehyde and limonene, the main constituents of D. surmandinum essential oil, inhibited all investigated cell growth in a dose- and time-dependent manner with IC50 values ranged from 0.25-5.0 mmol/L.

S-adenosyl-L-methionine (SAMe) reduces ethanol-induced mitochondrial toxicity and membrane damage in Hep G2 cells

1998 ◽  
Vol 28 ◽  
pp. 135
Author(s):  
M. Concari ◽  
B. Rebecchi ◽  
ME. Guiccìardi ◽  
F. Carubbi ◽  
P. Loria ◽  
...  
Keyword(s):  
Membrane Damage ◽  
Mitochondrial Toxicity ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

scholarly journals Antioxidant and Cytotoxic Properties of Essential Oils from Native Brazilian Lauraceae Species

2020 ◽  
pp. 1-8
Author(s):  
Fabiana Lima Silva ◽  
Amanda Espírito Santo ◽  
Paola Cristina Branco ◽  
Letícia Veras Costa-Lotufo ◽  
Maria Claúdia Marx Young ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Tumor ◽  
Cancer Cell Line ◽  
Major Constituent ◽  
Leaf Essential Oil ◽  
Hct 116 ◽  
Main Components

Analysis of the leaf and stem essential oils from three Brazilian Lauraceae species showed that the major constituent of O. odorifera was safrole (57.1 and 88.5% for leaves and stems, respectively) while for O. indecora leaves the major ones were the α-pinene (12.8%) and β-pinene (12.4%) andβ-bisabolol (12.2%) in the stems. For the third species, Persea venosa, the main components of leaf and stem oils were spathulenol (27.8 and 14.7%, respectively) and humulene epoxide II (11.3 and 5.1%, respectively). The bioactivity results indicated that the leaf essential oil from O. indecora was the most active for both radical scavenging activity (IC50=0.142mg/mL) and cytotoxicity against human tumor cells, with growth inhibitions for the colon tumoral line HCT-116 (100%)and breast cancer cell line MCF-7 (99.2%), at 5 μg/mL concentration. This activity might be related to the presence ofα-pinene and β-pinene in the leaf essential oil of O. indecora. However, further studies with the isolated compounds are necessary to fully understand these bioactivites.

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