Antifungal activity of lactobacilli in the sourdough

2021 ◽  
Vol 1 (3) ◽  
pp. 26-32
Author(s):  
M.N. Lokachuk ◽  
◽  
O.A. Savkina ◽  
E.A. Pavlovskaja ◽  
Yu.M. Frolova ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Penicillium Chrysogenum

В статье рассмотрена возможность применения заквасок для подавления плесневения хлеба. Целью работы являлось изучение влияния заквасок, приготовленных на чистых культурах заквасочных микроорганизмов, на скорость плесневения ржано-пшеничного и пшеничного хлебов. Исследования проводились в Санкт-Петербургском филиале ФГАНУ НИИХП в рамках темы госзадания № 2593-2014-0018 «Разработать научные основы формирования микробных композиций и биосистем на их основе, обеспечивающих высокое качество и безопасность хлебобулочных изделий». Объектами исследования являлись ржано-пшеничные и пшеничные хлеба, выработанные как с использованием заквасок, так и ускоренным способом. Для выявления влияния технологии приготовления хлеба на скорость развития плесени, проводили модельные опыты с заражением его стерильных ломтиков чистой культурой плесневых грибов Penicillium chrysogenum. В статье приведен обзор антимикробных метаболитов молочнокислых бактерий, обусловливающих их фунгицидную активность, рассмотрен механизм их воздействия на плесневые грибы. Установлено, что применение ржаной густой закваски в количестве 25 % муки в закваске имело наибольший фунгицидный эффект при хранении хлеба. Применение КМКЗ в количестве 20 % муки в закваске позволило замедлить плесневение на 1–1,5 суток по сравнению с контролем без закваски на подкисляющей добавке «Цитрасол». В результате проведенных исследований показано, что на скорость развития плесневых грибов оказывает влияние содержание уксусной кислоты в готовых изделиях, которая накапливается в процессе брожения разных видов заквасок.

scholarly journals S-ethyl ethanethiosulfinate, a derivative of allicin, induces metacaspase-dependent apoptosis through ROS generation in Penicillium chrysogenum

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Feilong Qi ◽  
Chen Zhang ◽  
Shanshan Jiang ◽  
Qian Wang ◽  
Kudelaidi Kuerban ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Penicillium Chrysogenum ◽  
Colorimetric Assay ◽  
Underlying Mechanism ◽  
Food Storage ◽  
Ros Generation ◽  
Confocal Immunofluorescence ◽  
High Efficient ◽  
High Volatility ◽  
Efficient Alternative

AbstractAllicin can be used as fumigant to protect food and cultural relics from fungal contamination because of its strong antifungal activity and the characteristics of high volatility and no residues. However, the obvious disadvantages such as high minimal inhibitory concentration and instability prevent it from wide application. In this study, a stable derivative of allicin, S-ethyl ethanethiosulfinate (ALE), was synthesized. We further explored its antifungal activity and apoptosis-inducing effect, as well as the underlying mechanism. ALE had an excellent capability of inhibiting spore germination and mycelial growth of Penicillium chrysogenum observed by inverted microscope and scanning electron microscopy. XTT colorimetric assay indicated ALE could reduce the cell viability obviously and IC50 was 0.92 μg/ml, only 1/42 of allicin (38.68 μg/ml). DHR 123 ROS Assay Kit, flow cytometry assay and confocal immunofluorescence revealed intercellular ROS generation and metacaspase-dependent apoptosis triggered by ALE, while antioxidant tocopherol could reverse ALE-induced cytotoxicity effect and metacaspase activation. These results indicate that ALE induces metacaspase-dependent apoptosis through ROS generation, thus possesses an effective antifungal activity. This new derivative of allicin might be developed as a high efficient alternative to the conventional fungicides for food storage and cultural relic protection.

Cloning, structural organization and regulation of expression of the Penicillium chrysogenum paf gene encoding an abundantly secreted protein with antifungal activity

Gene ◽  
1995 ◽  
Vol 167 (1-2) ◽  
pp. 167-171 ◽  
Author(s):  
Florentine Marx ◽  
Hubertus Haas ◽  
Markus Reindl ◽  
Georg Stöffler ◽  
Friedrich Lottspeich ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Penicillium Chrysogenum ◽  
Structural Organization ◽  
Secreted Protein ◽  
Regulation Of Expression ◽  
Gene Encoding

scholarly journals Screening of Lactobacillus plantarum with broad-spectrum antifungal activity and its application in preservation of golden-red apples

2020 ◽  
Vol 38 (No. 5) ◽  
pp. 315-322
Author(s):  
Kangning Li ◽  
Wenyi Zhang ◽  
Lai-Yu Kwok ◽  
Bilige Menghe
Keyword(s):  
Antifungal Activity ◽  
Lactobacillus Plantarum ◽  
Penicillium Chrysogenum ◽  
Fusarium Moniliforme ◽  
Low Ph ◽  
Penicillium Expansum ◽  
Economic Losses ◽  
Food Spoilage ◽  
Mycelium Growth ◽  
Overlay Method

Fungal food spoilage is a common problem that leads to both great economic losses and serious health problems. This study screened the antifungal activity of 137 Lactobacillus plantarum isolates against six common food spoilage indicator fungi using an overlay method and indicator strains of the species Aspergillus flavus, Fusarium moniliforme, Penicillium expansum, Penicillium chrysogenum, Cladosporium cladosporioides, and Aspergillus niger. Among Lactobacillus plantarum isolates, strain IMAU80174 was selected as the most effective based on the results of mycelium growth inhibition by its cell-free supernatant (CFS) and tolerance to simulated gastrointestinal juices and bile. The CFS of Lactobacillus plantarum IMAU80174 showed heat and protease resistance, and it was active only in a low pH environment. The application of the CFS to golden-red apples could slow down spoilage caused by inoculation of Penicillium expansum.

scholarly journals New Enantiomers of a Nor-Bisabolane Derivative and Two New Phthalides Produced by the Marine-Derived Fungus Penicillium chrysogenum LD-201810

2021 ◽  
Vol 12 ◽  
Author(s):  
Yan Ge ◽  
Wen-Li Tang ◽  
Qing-Rong Huang ◽  
Mao-Lian Wei ◽  
You-Zhi Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Penicillium Chrysogenum ◽  
Optical Resolution ◽  
Alternaria Solani ◽  
2D Nmr ◽  
Chiral Hplc ◽  
Excellent Activity ◽  
Marine Algal ◽  
Detailed Interpretation ◽  
Mcf 7

Marine-derived fungi are a treasure house for the discovery of structurally novel secondary metabolites with potential pharmaceutical value. In this study, a pair of new nor-bisabolane derivative enantiomers (±)−1 and two new phthalides (4 and 5), as well as four known metabolites, were isolated from the culture filtrate of the marine algal-derived endophytic fungus Penicillium chrysogenum LD-201810. Their structures were established by detailed interpretation of spectroscopic data (1D/2D NMR and ESI-MS). The optical resolution of compound (±)−1 by chiral HPLC successfully afforded individual enantiomers (+)−1 and (−)−1, and their absolute configurations were determined by TDDFT-ECD calculations. Compound (±)−1 represents the first example of bisabolane analogs with a methylsulfinyl substituent group, which is rare in natural products. All of the isolated compounds 1–7 were evaluated for their cytotoxic activity against A549, BT-549, HeLa, HepG2, MCF-7, and THP-1 cell lines, as well as for antifungal activity against four plant pathogenetic fungi (Alternaria solani, Botrytis cinerea, Fusarium oxysporum, and Valsa mali). Compound 2, a bisabolane-type sesquiterpenoid, was shown to possess excellent activity for control of B. cinerea with half-maximal inhibitory concentration (IC50) of 13.6 μg/mL, whereas the remaining investigated compounds showed either weak or no cytotoxic/antifungal activity in this study.

scholarly journals Antifungal activity of some metabolites of higher fungi (Basidiomycetes) - an overview

2014 ◽  
Vol 68 (4) ◽  
pp. 307-310 ◽  
Author(s):  
Teresa Florianowicz
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Penicillium Chrysogenum ◽  
Fungal Pathogens ◽  
Fusarium Culmorum ◽  
Alternaria Solani ◽  
Fungal Metabolites ◽  
Environmental Significance ◽  
Chemical Structures ◽  
Effective Activity

A series of compounds of different chemical structures showing antifungal activity were isolated from higher fungi (<em>Basidiomycetes</em>) fruit bodies. Among the microflora against which the examined metabolites showed effective activity, there are pathogenic organisms for people as well as for animals: <em>Candida albicans</em>, <em>Candida tropicalis</em>, <em>Rhodotorula rubra</em>, <em>Aspergillus fumigatus</em> and pathogens attacking plants: <em>Penicillium chrysogenum</em>, <em>Botrytis cinerea</em>, <em>Alternaria solani</em>, <em>Fusarium culmorum</em>, <em>Trichoderma lignorum</em> and <em>Verticillium dahlae</em>. Searching for fungal metabolites having antifungal activity creates possibilities of using them against a range of fungal pathogens of clinical, agronomic and environmental significance.

scholarly journals Inhibition of the Growth and Development of Sclerotinia sclerotiorum (Lib.) De Bary by Combining Azoxystrobin, Penicillium chrysogenum VKM F-4876d, and Bacillus Strains

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2520
Author(s):  
Vera V. Yaderets ◽  
Nataliya V. Karpova ◽  
Elena V. Glagoleva ◽  
Alexander I. Ovchinnikov ◽  
Kseniya S. Petrova ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Penicillium Chrysogenum ◽  
Growth And Development ◽  
Crop Protection ◽  
Pathogenic Fungi ◽  
Fungal Cell ◽  
Bacillus Strains

Sclerotinia sclerotiorum (Lib.) de Bary is a plant pathogen with a wide host range, which causes significant yield and storage losses of edible roots and other plant products. Due to its ability to sclerotia formation, the efficient control of this pathogen is complicated. The study of five Bacillus strains (B. subtilis VKM B-3154D, VKM B-3155D, VKM B-3505D, VKM B-2998D, and B. amyloliquefaciens VKM B-3153D) showed their ability to produce polyene antibiotics suppressing the growth and development of plant pathogenic fungi. The maximum concentration of polyene compounds was revealed for B. subtilis VKM B-2998D. A high in vitro antifungal activity of a dry mycelium biomass (DMP) of Penicillium chrysogenum VKM F-4876D, B. subtilis VKM B-2998D, and their combination has been demonstrated in relation to S. sclerotiorum. A combined application of DMP (0.3 g/L) and azoxystrobin at low dosage (2.5 mg/L) showed a high suppressing activity towards S. sclerotiorum (100% growth inhibition) including inhibition of a sclerotia formation that may be useful for the development of efficient methods of crop protection against this plant pathogen. A high performance liquid chromatography (HPLC) analysis of DMP revealed the presence of mevastatin suggesting the mechanism of the DMP antifungal activity is based on the blocking of the ergosterol (the main component of fungal cell walls) biosynthesis. The results of the study provide a prerequisite to the development of biopreparations to control S. sclerotiorum, whose use may provide a reduction of concentrations of fungicides used in agriculture and the corresponding reduction of their negative xenobiotic impact on the environment and recovery of the ecological balance in the soil.

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