Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus

2020 ◽  
Vol 145 ◽  
pp. 111754
Author(s):  
Liuming Xie ◽  
Mingyue Shen ◽  
Pingwei Wen ◽  
Yuzhen Hong ◽  
Xuan Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Cyclocarya Paliurus

Antioxidant Activity and Protective Effect against Oxidative Stress on Hippocampal HT22 Cells of Tea Seed Oil

2018 ◽  
Vol 24 (1) ◽  
pp. 53-59
Author(s):  
Jong Min Kim ◽  
Seon Kyeong Park ◽  
Jin Yong Kang ◽  
Seong-kyeong Bae ◽  
Ga-Hee Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Seed Oil ◽  
Ht22 Cells ◽  
Tea Seed Oil

scholarly journals Antioxidant Effects and Cytoprotective Potentials of Herbal Tea against H2O2-Induced Oxidative Damage by Activating Heme Oxygenase1 Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Ke-Xin Zhang ◽  
Jian-Bin Tan ◽  
Cheng-Liang Xie ◽  
Rong-Bo Zheng ◽  
Xiao-Dan Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Functional Foods ◽  
Cell Damage ◽  
Damage Model ◽  
Enrichment Analysis ◽  
Protective Effects ◽  
Herbal Tea ◽  
Antioxidant Effects

Herbal tea with antioxidant ingredients has gained increasing attention in the field of functional foods due to their amelioration potential in aging-related diseases. Wanglaoji herbal tea (WHT) is a kind of traditional beverage made from herbal materials. This study was performed to investigate its antioxidant activity and identify its protective effect on a H2O2-induced cell damage model. In this study, we identified six kinds of phenolic acids with antioxidant activity in WHT, among which rosmarinic acid had the highest content and the highest contribution ratio to the antioxidant activity of WHT. Moreover, compared with the H2O2-induced damage group, the WHT treatment group can significantly increase the viability of cells and decrease the ratio of senescence-associated β-galactosidase-positive cells, intracellular malondialdehyde levels, and the percentage of G1 phase. Furthermore, enrichment analysis of differentially expressed genes revealed that heme oxygenase1 (HMOX1) was a key gene for protective effect of WHT on oxidative stress-induced cell damage. Thus, WHT exerted protective effects not only by scavenging reactive oxygen species but also by inducing the expression of cytoprotective genes by activating the HMOX1 pathway, which showed that WHT had a potential of promoting health by reducing oxidative stress-induced cell damage.

scholarly journals Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5149
Author(s):  
Roberto O. Ybañez-Julca ◽  
Daniel Asunción-Alvarez ◽  
Ivan M. Quispe-Díaz ◽  
Javier Palacios ◽  
Jorge Bórquez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
High Performance ◽  
Mangifera Indica ◽  
Folk Medicine ◽  
In Vitro Assays ◽  
Antispasmodic Activity ◽  
Intestinal Segments

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

Antioxidant activity and PC12 cell protective effect of onion flesh and peel(Allium cepa L.) fraction on oxidative stress

2015 ◽  
Vol 49 (2) ◽  
pp. 83-95 ◽  
Author(s):  
Seon-Kyeong Park ◽  
◽  
Dong-Eun Jin ◽  
Chang-Hyeon Park ◽  
Tae-Wan Seung ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Allium Cepa ◽  
Pc12 Cell ◽  
Allium Cepa L

scholarly journals Protective Effect of Merbau (Intsia bijuga) Extract on Hydrogen Peroxide-Treated HaCaT Human Keratinocytes and Its Formulation as Antioxidant Cream

2020 ◽  
Vol 27 (1) ◽  
pp. 131-138
Author(s):  
Leonny Yulita Hartiadi ◽  
Agnes Anania Triavika sahamastuti ◽  
Cynthia Valentina Chandra ◽  
Erlina Febriani ◽  
Shakila Angyaja Adiyanto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Skin Aging ◽  
Ph Values ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Dried Extract ◽  
Evaluation Parameters

Background: The excessive generation of ROS in the skin results in oxidative stress that can contribute to premature skin aging, inflammation, and skin carcinogenesis. To prevent these detrimental effects, the development of herbal medicine with a potent antioxidant activity into cosmetic products is required. This study aims to formulate cream that contains a safe and effective concentration of merbau (Intsia bijuga), which has been shown to have a strong antioxidant activity. Methods: Powdered merbau wood was macerated with methanol and the dried extract was evaluated for its cytotoxic effect and antioxidant activity on human keratinocytes cell line using MTS assay. Five cream formulations containing the extract were made and subjected to stability and physical evaluations, including organoleptic, types of cream, pH, viscosity, and homogeneity. Results: Cytotoxicity assay revealed that merbau extracts had an IC50 of 181.3 μg/mL (95% confidence interval (CI): 165.4 - 200.1 µg/mL). At a concentration of 31.25 μg/mL, the extract exhibited a protective effect against H2O2-induced oxidative stress, comparable to vitamin E. Five cream formulas that were developed demonstrated good physical properties that fulfilled the evaluation parameters, including o/w type of cream, homogenous, and stable based on centrifugation and freeze-thaw cycle tests. The pH values were between 5.65 ± 0.067 - 7.4 ± 0.050, while the viscosity values were between 131 ± 1.249 - 56,011 ± 2,729.27 mPa.s. All cream formulas exhibited shear-thinning properties upon increasing shear stress. Conclusion: Overall, this study has successfully formulated several cream formulations containing merbau extract at a concentration that shows antioxidant activity.

scholarly journals In vitro antioxidant activity and protective effect of Hertia cheirifolia L. n-butanol extract against liver and heart mitochondrial oxidative stress in rat

2020 ◽  
Vol 7 (1) ◽  
pp. 33-45
Author(s):  
Mouna Menakh ◽  
Djahida Mahdi ◽  
Saber Boutellaa ◽  
Amar Zellagui ◽  
Mesbah Lahouel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Reducing Power ◽  
Mitochondrial Oxidative Stress ◽  
Butanol Extract ◽  
Potential Source ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

AbstractThe current study investigated the protective effect of Hertia cheirifolia L. n-butanol extract on oxidative stress in vitro by measuring lipid peroxidation (MDA) level and superoxide anion (O2•-) production in liver and heart mitochondria of rat. In addition, the antioxidant potential of H. cheirifolia n-butanol extract was evaluated by using five methods: ABTS•+, O2•-, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. The results indicated that n-butanol extract contained large amounts of total phenolics (203.52±1.81 mg GAE/g) and flavonoids (104.86±0.57 mg QE/g), and had an interesting antioxidant activity and protective effect on mitochondrial oxidative stress. Therefore, H. cheirifolia n-butanol extract may serve as potential source of natural antioxidant for pharmaceutical applications.

Sign in / Sign up

Export Citation Format

Share Document