scholarly journals Forensic Assessment of Genotoxic Effects of Lead Metal on leaf Phragmites australis Using Random Amplified Polymorphic DNA (RAPD-PCR) Markers

2021 ◽  
Keyword(s):  
Phragmites Australis ◽  
Random Amplified Polymorphic Dna ◽  
Forensic Assessment ◽  
Pcr Markers ◽  
Genotoxic Effects ◽  
Rapd Pcr ◽  
Lead Metal

Intensive Linkage Mapping in a Wasp (Bracon hebetor) and a Mosquito (Aedes aegypti) With Single-Strand Conformation Polymorphism Analysis of Random Amplified Polymorphic DNA Markers

Genetics ◽  
1996 ◽  
Vol 143 (4) ◽  
pp. 1727-1738
Author(s):  
Michael F Antolin ◽  
Christopher F Bosio ◽  
Julie Cotton ◽  
William Sweeney ◽  
Michael R Strand ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Random Amplified Polymorphic Dna ◽  
Single Strand Conformation Polymorphism ◽  
Sscp Analysis ◽  
Agarose Gel ◽  
Linkage Maps ◽  
Agarose Gel Electrophoresis ◽  
Pcr Markers ◽  
Rapd Pcr ◽  
Conformation Polymorphism

Abstract The use of random amplified polymorphic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated linkage maps. However, when analyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing the amount of linkage information obtained. We describe the use of single strand conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps in a haplodiploid parasitic wasp Bracon (Habrobracon) hebetor and a diploid mosquito, Aedes aegypti. RAPD-SSCP analysis revealed segregation of codominant alleles at markers that appeared to segregate as dominant (band presence/band absence) markers or appeared invariant on agarose gels. Our SSCP protocol uses silver staining to detect DNA fractionated on large thin polyacrylamide gels and reveals more polymorphic markers than agarose gel electrophoresis. In B. hebetor, 79 markers were mapped with 12 RAPD primers in six weeks; in A. aegypti, 94 markers were mapped with 10 RAPD primers in five weeks. Forty-five percent of markers segregated as codominant loci in B. hebetor, while 11% segregated as codominant loci in A. aegypti. SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of constructing intensive linkage maps of many species.

scholarly journals Assessment of genotoxic effects of copper on Cucumber Plant (Cucumis sativus L.) using Random Amplified Polymorphic DNA (RAPD-PCR) markers

2014 ◽  
Vol 8 (3) ◽  
pp. 12-19
Author(s):  
Akeel H. Ali AL-Assie
Keyword(s):  
Cucumis Sativus ◽  
Copper Sulfate ◽  
Genomic Dna ◽  
Random Amplified Polymorphic Dna ◽  
Cucumber Plant ◽  
High Concentration ◽  
Genotoxic Effects ◽  
Polluted Soils ◽  
Cucumis Sativus L ◽  
Rapd Pcr

The aim of this study was to evaluate the genotoxic effects of copper on Cucumis sativus L., fife levels of copper sulfate as aqueous solutions were added as a total concentrate of the soil using the concentrations (100, 200, 300, 400, 500) milligrams per one kilogram of soil, the control soil were using without additional copper sulfate. Random amplified polymorphic DNA (RAPD) technique was used for detection of genotoxic produced by this metal. Twenty one primers were used, of which three did not amplify, three gave extremely faint and ambiguous bands and the rest of fifteen primers generated single and polymorphic bands. All genomic DNA of Cucumis sativus L. exposed to copper sulfate solutions displayed polymorphic fragments which were not detectable in DNA of unexposed plants, but high concentration (500 mg/kg soil) of copper sulfate had the strongest effect on genomic DNA of Cucumis sativus L. when compared with other concentrations. Thus, this study confirmed that RAPD markers, as a fast, and simple technique can be used for detection of genotoxic effects of copper on Cucumis sativus L. plants which are growing in heavy metal polluted soils.

scholarly journals Oenological Potential of Autochthonous Saccharomyces cerevisiae Yeast Strains from the Greek Varieties of Agiorgitiko and Moschofilero

Beverages ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 27
Author(s):  
Dimitrios Kontogiannatos ◽  
Vicky Troianou ◽  
Maria Dimopoulou ◽  
Polydefkis Hatzopoulos ◽  
Yorgos Kotseridis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcoholic Fermentation ◽  
Ethanol Tolerance ◽  
Random Amplified Polymorphic Dna ◽  
Yeast Strains ◽  
Rapd Pcr ◽  
Autochthonous Yeast ◽  
Polymerase Chain ◽  
Grape Varieties ◽  
H2s Production

Nemea and Mantinia are famous wine regions in Greece known for two indigenous grape varieties, Agiorgitiko and Moschofilero, which produce high quality PDO wines. In the present study, indigenous Saccharomyces cerevisiae yeast strains were isolated and identified from spontaneous alcoholic fermentation of Agiorgitiko and Moschofilero musts in order to evaluate their oenological potential. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) recovered the presence of five distinct profiles from a total of 430 yeast isolates. The five obtained strains were evaluated at microvinifications trials and tested for basic oenological and biochemical parameters including sulphur dioxide and ethanol tolerance as well as H2S production in sterile grape must. The selected autochthonous yeast strains named, Soi2 (Agiorgitiko wine) and L2M (Moschofilero wine), were evaluated also in industrial (4000L) fermentations to assess their sensorial and oenological characteristics. The volatile compounds of the produced wines were determined by GC-FID. Our results demonstrated the feasibility of using Soi2 and L2M strains in industrial fermentations for Agiorgitiko and Moschofilero grape musts, respectively.

scholarly journals Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients

2010 ◽  
Vol 59 (3) ◽  
pp. 207-212 ◽  
Author(s):  
M.I. ABOU-DOBARA ◽  
M.A. DEYAB ◽  
E.M. ELSAWY ◽  
H.H. MOHAMED
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Random Amplified Polymorphic Dna ◽  
Test Methods ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Rapd Pcr

Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P. aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five for K. pneumoniae and five for P. aeruginosa respectively). In this study, differentiation of strains of E. coli, K. pneumoniae and P. aeruginosa from nosocomial infection was possible with the use of RAPD.

scholarly journals The Ancient Neapolitan Sweet Lime and the Calabrian Lemoncetta Locrese Belong to the Same Citrus Species

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 113 ◽  
Author(s):  
Domenico Cautela ◽  
Maria Luisa Balestrieri ◽  
Sara Savini ◽  
Anna Sannino ◽  
Giovanna Ferrari ◽  
...  
Keyword(s):  
Dna Fingerprint ◽  
Citrus Fruits ◽  
Citrus Species ◽  
Pcr Markers ◽  
Chiral Compounds ◽  
Sweet Lime ◽  
Rapd Pcr ◽  
Volatile Organic ◽  
Time Specific ◽  
First Time

“Neapolitan limmo” is an ancient and rare sweet Mediterranean lime, now almost extinct but used until a few decades ago for the production of a fragrant liqueur called the “four citrus fruits”. The objective of this work was to compare, through the use of chemical (flavonoids, volatile organic compounds, and chiral compounds) and molecular (DNA fingerprint based on RAPD-PCR) markers, the residual population of Neapolitan limmo with other populations of sweet limes, identified in Calabria and known as “lemoncetta Locrese”. We report for the first time specific botanical characteristics of the two fruits and unequivocally show that the ancient sweet Mediterranean limes Neapolitan limmo and lemoncetta Locrese are synonyms of the same Citrus species. Owing to the biodiversity conserved in their places of origin, it will now be possible to recover, enhance and implement the use of this ancient sweet lime for agro-industrial purposes.

scholarly journals Interlaboratory Study on Thermal Cycler Performance in Controlled PCR and Random Amplified Polymorphic DNA Analyses

2001 ◽  
Vol 47 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Ginny C Saunders ◽  
Juliet Dukes ◽  
Helen C Parkes ◽  
Johanne H Cornett
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Positive Sample ◽  
Interlaboratory Study ◽  
Pcr Assay ◽  
Single Product ◽  
The United Kingdom ◽  
Rapd Pcr ◽  
Block Temperature ◽  
Thermal Cycler ◽  
Correct Target

Abstract Background: Intercomparisons of PCR-based data between laboratories require an assurance of assay reproducibility. We performed an interlaboratory study to investigate the contribution made by a variety of thermal cyclers to PCR performance as measured by interblock reproducibility and intrablock repeatability. Methods: Two standardized assays designed to minimize the introduction of non-thermal-cycler-dependent variations were evaluated by 18 laboratories in the United Kingdom, using 33 thermal cyclers of various makes and models. We used a single-product (590 bp) PCR, established in our laboratory as a robust and specific reaction. The second reaction, a multiproduct random amplified polymorphic DNA (RAPD) PCR, was known to be more susceptible to small changes in block temperature and was therefore considered a way of assessing block uniformity with respect to temperature. Assay repeatability data were analyzed with respect to temperature calibration status, the type of temperature control mechanism, thermal cycler age, and the presence of oil overlay or heated lid systems. Results: All (100%) of the laboratories produced the correct target for the single-product PCR assay, although substantial variation in yield in replicate reactions was observed in 9.4% of these. The RAPD reaction generated results that varied extensively both within the same block and between different thermal cyclers. For eight replicates of a positive sample, 88% intrablock repeatability was demonstrated in calibrated thermal cyclers, which decreased to 63% in noncalibrated instruments. Conclusions: Irrespective of the make and model of thermal cycler, temperature-calibrated instruments consistently generated more repeatable RAPD data than noncalibrated instruments. Guidelines are offered on optimizing and monitoring thermal cycler performance.

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