scholarly journals pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages

2021 ◽  
Vol 12 ◽  
pp. 1127-1139
Author(s):  
Karishma Berta Cotta ◽  
Sarika Mehra ◽  
Rajdip Bandyopadhyaya
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Drug Release ◽  
Iron Oxide Nanoparticles ◽  
Drug Loading ◽  
Drug Carriers ◽  
Free Drug ◽  
Release Profile ◽  
Oxide Nanoparticles ◽  
Drug Coating

Nanoparticle deployment in drug delivery is contingent upon controlled drug loading and a desired release profile, with simultaneous biocompatibility and cellular targeting. Iron oxide nanoparticles (IONPs), being biocompatible, are used as drug carriers. However, to prevent aggregation of bare IONPs, they are coated with stabilizing agents. We hypothesize that, zwitterionic drugs like norfloxacin (NOR, a fluoroquinolone) can manifest dual functionality – nanoparticle stabilization and antibiotic activity, eliminating the need of a separate stabilizing agent. Since these drugs have different charges, depending on the surrounding pH, drug loading enhancement could be pH dependent. Hence, upon synthesizing IONPs, they were coated with NOR, either at pH 5 (predominantly as cationic, NOR+) or at pH 10 (predominantly as anionic, NOR−). We observed that, drug loading at pH 5 exceeded that at pH 10 by 4.7–5.7 times. Furthermore, only the former (pH 5 system) exhibited a desirable slower drug release profile, compared to the free drug. NOR-coated IONPs also enable a 22 times higher drug accumulation in macrophages, compared to identical extracellular concentrations of the free drug. Thus, lowering the drug coating pH to 5 imparts multiple benefits – improved IONP stability, enhanced drug coating, higher drug uptake in macrophages at reduced toxicity and slower drug release.

scholarly journals Lyophilized Iron Oxide Nanoparticles Encapsulated in Amphotericin B: A Novel Targeted Nano Drug Delivery System for the Treatment of Systemic Fungal Infections

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 247
Author(s):  
Pavan Balabathula ◽  
Sarah Garland Whaley ◽  
Dileep R. Janagam ◽  
Nivesh K. Mittal ◽  
Bivash Mandal ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Drug Release ◽  
Amphotericin B ◽  
Iron Oxide Nanoparticles ◽  
Delivery System ◽  
Fungal Infections ◽  
Drug Loading ◽  
Laser Scanning Microscopy ◽  
Oxide Nanoparticles ◽  
Burst Release

We formulated and tested a targeted nanodrug delivery system to help treat life-threatening invasive fungal infections, such as cryptococcal meningitis. Various designs of iron oxide nanoparticles (IONP) (34–40 nm) coated with bovine serum albumin and coated and targeted with amphotericin B (AMB-IONP), were formulated by applying a layer-by-layer approach. The nanoparticles were monodispersed and spherical in shape, and the lead formulation was found to be in an optimum range for nanomedicine with size (≤36 nm), zeta potential (−20 mV), and poly dispersity index (≤0.2), and the drug loading was 13.6 ± 6.9 µg of AMB/mg of IONP. The drug release profile indicated a burst release of up to 3 h, followed by a sustained drug release of up to 72 h. The lead showed a time-dependent cellular uptake in C. albicans and C. glabrata clinical isolates, and exhibited an improved efficacy (16–25-fold) over a marketed conventional AMB-deoxycholate product in susceptibility testing. Intracellular trafficking of AMB-IONP by TEM and confocal laser scanning microscopy confirmed the successful delivery of the AMB payload at and/or inside the fungal cells leading to potential therapeutic advantages over the AMB-deoxycholate product. A short-term stability study at 5 °C and 25 °C for up to two months showed that the lyophilized form was stable.

scholarly journals Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin

2021 ◽  
Vol 14 (5) ◽  
pp. 405
Author(s):  
Chiara Turrina ◽  
Sonja Berensmeier ◽  
Sebastian P. Schwaminger
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Drug Loading ◽  
Binding Potential ◽  
Oxide Nanoparticles ◽  
Release Process ◽  
Free Peptide ◽  
High Binding ◽  
Drug Delivery Carrier

New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on Escherichia coli show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.

scholarly journals Curcumin-Loaded Iron Oxide Nanoparticles Coated with Sodium Alginate and Hydroxyapatite and Their Cytotoxic Effects Against the HT-29 and MCF-7 Cancer Cell Lines

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Masoomeh Nobahari ◽  
Kahin Shahanipour ◽  
Soheil Fatahian ◽  
Ramesh Monajemi
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Drug Release ◽  
Iron Oxide Nanoparticles ◽  
Encapsulation Efficiency ◽  
Oxide Nanoparticles ◽  
Cytotoxic Effects ◽  
Ht 29 ◽  
Mcf 7

Background: Curcumin, a bioactive component of Curcuma langa, has been investigated for its anti-proliferative effects against various cancer cell lines. Although results are very promising, the poor water solubility and low bioavailability of curcumin are its main limitations for clinical application. Objectives: The purpose of this study was to develop a drug delivery system, consisting of hydroxyapatite (HAp) polymer and sodium alginate (NaAlg), covering the magnetic core of iron oxide nanoparticles (IONPs), and loaded with curcumin in order to enhance its bioavailability and therapeutic efficacy. Methods: In this study, IONPs were prepared by the co-precipitation method and coated with HAp and NaAlg. The nanoparticles (NPs) were characterized by X-ray diffraction, Fourier Transform Infrared Spectroscopy (FTIR), and electron microscopy (TEM and SEM). Encapsulation efficiency and curcumin loading rate were examined. Drug release rate was also measured in vitro at pH = 7.5 and 5.5. The toxicity of curcumin-loaded NPs and free curcumin was evaluated against HT-29 and MCF-7 cancer cells. Results: The assessment of physicochemical characteristics showed the synthesis of spherical particles with nanometer sizes (5 - 7 nm) and a high encapsulation efficiency (84.16 ± 3.51 %) and drug loading capacity (21.03 ± 0.87%). Maximum drug release was obtained at pH = 5.5. Iron oxide nanoparticles showed no significant cytotoxic effects. Curcumin-loaded coated IONPs showed a higher toxicity against HT-29 and MCF-7 cancer cells compared to free curcumin. Conclusions: This in vitro study showed that the encapsulation of curcumin, as a potent herbal drug, into IONPs enhanced its bioavailability, suggesting the NPs as an efficient vehicle for targeted drug delivery in cancer treatment.

scholarly journals Magnetic Driven Nanocarriers for pH-Responsive Doxorubicin Release in Cancer Therapy

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 333 ◽  
Author(s):  
João Nogueira ◽  
Sofia F. Soares ◽  
Carlos O. Amorim ◽  
João S. Amaral ◽  
Cláudia Silva ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Drug Loading ◽  
Drug Carriers ◽  
Release Profile ◽  
Loading Capacity ◽  
Drug Release Profile ◽  
Blood Ph ◽  
Alternative Drug ◽  
Doxorubicin Release

Doxorubicin is one of the most widely used anti-cancer drugs, but side effects and selectivity problems create a demand for alternative drug delivery systems. Herein we describe a hybrid magnetic nanomaterial as a pH-dependent doxorubicin release carrier. This nanocarrier comprises magnetic iron oxide cores with a diameter of 10 nm, enveloped in a hybrid material made of siliceous shells and ĸ-carrageenan. The hybrid shells possess high drug loading capacity and a favorable drug release profile, while the iron oxide cores allows easy manipulation via an external magnetic field. The pH responsiveness was assessed in phosphate buffers at pH levels equivalent to those of blood (pH 7.4) and tumor microenvironment (pH 4.2 and 5). The nanoparticles have a loading capacity of up to 12.3 wt.% and a release profile of 80% in 5 h at acidic pH versus 25% at blood pH. In vitro drug delivery tests on human breast cancer and non-cancer cellular cultures have shown that, compared to the free drug, the loaded nanocarriers have comparable antiproliferative effect but a less intense cytotoxic effect, especially in the non-cancer cell line. The results show a clear potential for these new hybrid nanomaterials as alternative drug carriers for doxorubicin.

scholarly journals Pulse Magnetic Fields Induced Drug Release from Gold Coated Magnetic Nanoparticle Decorated Liposomes

2020 ◽  
Vol 6 (4) ◽  
pp. 52
Author(s):  
Basanta Acharya ◽  
Viktor Chikan
Keyword(s):  
Iron Oxide ◽  
Drug Release ◽  
Magnetic Fields ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Magnetic Particles ◽  
Drug Loading ◽  
Oxide Nanoparticles ◽  
Efficient Coupling ◽  
Model Drug

Magnetic nanoparticle-assisted drug release from liposomes is an important way to enhance the functionality/usefulness of liposomes. This work demonstrates an approach how to integrate magnetic nanoparticles with liposomes with the assistance of gold–thiol chemistry. The gold coated magnetic particles cover the thiolated liposomes from the outside, which removes the competition of the drug molecules and the triggering magnetic particles to free the inner space of the liposomes when compared to previous magneto liposome formulations. The liposome consists of dipalmitoyl phosphatidylcholine (DPPC) combined with distearoylphosphatidylcholine (DSPC) in addition to regular cholesterol or cholesterol-PEG-SH. Permeability assays and electron microscopy images show efficient coupling between the liposomes and nanoparticles in the presence of thiol groups without compromising the functionality of the liposomes. The nanoparticles such as gold nanoparticles, gold coated iron oxide nanoparticles and bare iron oxide nanoparticles are added following the model drug encapsulation. The efficient coupling between the gold coated nanoparticles (NPs) and the thiolate liposomes is evidenced by the shift in transition temperature of the thiolated liposomes. The addition of magnetically triggerable nanoparticles externally makes the entire interior of liposomes available for drug loading. The drug release efficiencies of these liposomes/NPs complexes were compared under exposure to pulsed magnetic fields. The results indicate up to 20% of the drug can be released in short time, which is comparable in efficiency to previous studies performed when magnetic NPs were located inside liposomes. Interestingly, the liposomes were found to exhibit variations in release efficiency based on different dilution media which is attributed to an osmotic pressure effect on liposomal stability.

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

scholarly journals Anodic Titanium Dioxide Nanotubes for Magnetically Guided Therapeutic Delivery

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Morteza Hasanzadeh Kafshgari ◽  
Delf Kah ◽  
Anca Mazare ◽  
Nhat Truong Nguyen ◽  
Monica Distaso ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hela Cells ◽  
Release Kinetics ◽  
Drug Loading ◽  
Oxide Nanoparticles ◽  
Site Specific ◽  
Therapeutic Drugs ◽  
Wide Range

Abstract Hollow titanium dioxide (TiO2) nanotubes offer substantially higher drug loading capacity and slower drug release kinetics compared to solid drug nanocarriers of comparable size. In this report, we load TiO2 nanotubes with iron oxide nanoparticles to facilitate site-specific magnetic guidance and drug delivery. We generate magnetic TiO2 nanotubes (TiO2NTs) by incorporating a ferrofluid containing Ø ≈ 10 nm iron oxide nanoparticles in planar sheets of weakly connected TiO2 nanotubes. After thermal annealing, the magnetic tubular arrays are loaded with therapeutic drugs and then sonicated to separate the nanotubes. We demonstrate that magnetic TiO2NTs are non-toxic for HeLa cells at therapeutic concentrations (≤200 µg/mL). Adhesion and endocytosis of magnetic nanotubes to a layer of HeLa cells are increased in the presence of a magnetic gradient field. As a proof-of-concept, we load the nanotubes with the topoisomerase inhibitor camptothecin and achieve a 90% killing efficiency. We also load the nanotubes with oligonucleotides for cell transfection and achieve 100% cellular uptake efficiency. Our results demonstrate the potential of magnetic TiO2NTs for a wide range of biomedical applications, including site-specific delivery of therapeutic drugs.

scholarly journals A comprehensive study on 2D, 3D and solid tumor environment to explore a multifunctional biogenic nanoconjugate

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
B. S. Unnikrishnan ◽  
G. U. Preethi ◽  
T. T. Sreelekha
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Tumor Burden ◽  
Oxide Nanoparticles ◽  
Cell Internalization ◽  
Tumor Environment ◽  
D Cell ◽  
Cancer Spheroids ◽  
Comprehensive Study

AbstractEmergence of nanotechnology created a drastic change in the field of cancer therapy due to their unique features in drug delivery and imaging. Polysaccharide based nanoparticles have received extensive attention in recent years as promising nanoparticle mediated drug delivery systems. Polysaccharides are endorsed with versatile merits including high drug encapsulation efficiency, efficient drug protection against chemical or enzymatic degradation, unique ability to create a controlled release and cellular internalization. In the current study, we have fabricated doxorubicin-loaded carboxymethylated PST001 coated iron oxide nanoparticles (DOX@CM-PST-IONPs) for better management of cancer. CM-PST coated iron oxide nanoparticles co-encapsulated with chemotherapeutic drug doxorubicin, can be utilized for targeted drug delivery. Biocompatible and non-toxic nanoconjugates was found to be effective in both 2-D and 3-D cell culture system with efficient cancer cell internalization. The bench-marked potential of CM-PIONPs to produce reactive oxygen species makes it a noticeable drug delivery system to compact neoplasia. These nanoconjugates can lay concrete on a better way for the elimination of cancer spheroids and tumor burden.

Sign in / Sign up

Export Citation Format

Share Document