scholarly journals Dielectrophoresis of gold nanoparticles conjugated to DNA origami structures

2016 ◽  
Vol 7 ◽  
pp. 948-956 ◽  
Author(s):  
Anja Henning-Knechtel ◽  
Matthew Wiens ◽  
Mathias Lakatos ◽  
Andreas Heerwig ◽  
Frieder Ostermaier ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Self Assembly ◽  
Metallic Nanoparticles ◽  
Linear Chain ◽  
Construction Materials ◽  
Dna Origami ◽  
Hybrid Structures ◽  
Dna Nanostructures ◽  
Electrode Structure ◽  
Electrical Field Strength

DNA nanostructures are promising construction materials to bridge the gap between self-assembly of functional molecules and conventional top-down fabrication methods in nanotechnology. Their positioning onto specific locations of a microstructured substrate is an important task towards this aim. Here we study manipulation and positioning of pristine and of gold nanoparticle-conjugated tubular DNA origami structures using ac dielectrophoresis. The dielectrophoretic behavior was investigated employing fluorescence microscopy. For the pristine origami, a significant dielectrophoretic response was found to take place in the megahertz range, whereas, due to the higher polarizability of the metallic nanoparticles, the nanoparticle/DNA hybrid structures required a lower electrical field strength and frequency for a comparable trapping at the edges of the electrode structure. The nanoparticle conjugation additionally resulted in a remarkable alteration of the DNA structure arrangement. The growth of linear, chain-like structures in between electrodes at applied frequencies in the megahertz range was observed. The long-range chain formation is caused by a local, gold nanoparticle-induced field concentration along the DNA nanostructures, which in turn, creates dielectrophoretic forces that enable the observed self-alignment of the hybrid structures.

Control of the stepwise assembly–disassembly of DNA origami nanoclusters by pH stimuli-responsive DNA triplexes

Nanoscale ◽  
2019 ◽  
Vol 11 (39) ◽  
pp. 18026-18030 ◽  
Author(s):  
Shuo Yang ◽  
Wenyan Liu ◽  
Risheng Wang
Keyword(s):  
Self Assembly ◽  
Dna Origami ◽  
Dna Nanostructures ◽  
Stimuli Responsive ◽  
Dna Triplexes ◽  
Stepwise Assembly

We demonstrate the pH-regulated, multistep self-assembly of DNA nanostructures by employing DNA triplexes as dynamic linkers in a stepwise, selective, and reversible fashion.

Hybrid Structures for Surface-Enhanced Raman Scattering: DNA Origami/Gold Nanoparticle Dimer/Graphene

Small ◽  
2016 ◽  
Vol 12 (39) ◽  
pp. 5458-5467 ◽  
Author(s):  
Julia Prinz ◽  
Aleksandar Matković ◽  
Jelena Pešić ◽  
Radoš Gajić ◽  
Ilko Bald
Keyword(s):  
Raman Scattering ◽  
Gold Nanoparticle ◽  
Surface Enhanced Raman Scattering ◽  
Dna Origami ◽  
Hybrid Structures ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals DNA Origami “Quick” Refolding inside of a Micron-Sized Compartment

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 8 ◽  
Author(s):  
Taiki Watanabe ◽  
Yusuke Sato ◽  
Hayato Otaka ◽  
Ibuki Kawamata ◽  
Satoshi Murata ◽  
...  
Keyword(s):  
Self Assembly ◽  
Average Diameter ◽  
Test Tube ◽  
Dna Origami ◽  
Bulk Solution ◽  
Dna Nanostructures ◽  
Force Microscopy ◽  
Annealing Conditions ◽  
Atomic Force ◽  
Life Phenomena

Investigations into the refolding of DNA origami leads to the creation of reconstructable nanostructures and deepens our understanding of the sustainability of life. Here, we report the refolding of the DNA origami structure inside a micron-sized compartment. In our experiments, conventional DNA origami and truss-type DNA origami were annealed and purified to remove the excess staples in a test tube. The DNA origami was then encapsulated inside of a micron-sized compartment of water-in-oil droplets, composed of neutral surfactants. The re-annealing process was then performed to initiate refolding in the compartment. The resulting 100-nm-sized DNA nanostructures were observed using atomic force microscopy (AFM), and the qualities of their structures were evaluated based on their shape. We found that the refolding of the DNA origami structure was favored inside the droplets compared with refolding in bulk solution. The refolded structures were able to fold even under “quick” one-minute annealing conditions. In addition, the smaller droplets (average diameter: 1.2 µm) appeared to be more advantageous for the refolding of the origamis than larger droplets. These results are expected to contribute to understanding the principles of life phenomena based on multimolecular polymer self-assembly in a micron-sized compartment, and for the production and maintenance of artificially designed molecules.

scholarly journals DNA Ring Motif with Flexible Joints

Micromachines ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 987 ◽  
Author(s):  
Shiyun Liu ◽  
Satoshi Murata ◽  
Ibuki Kawamata
Keyword(s):  
Self Assembly ◽  
Dna Origami ◽  
Molecular Devices ◽  
Dna Nanostructures ◽  
Geometric Complexity ◽  
Flexible Joints ◽  
Self Assembling ◽  
Complementary Sequences ◽  
Novel Method ◽  
Ring Motif

The invention of DNA origami has expanded the geometric complexity and functionality of DNA nanostructures. Using DNA origami technology, we develop a flexible multi-joint ring motif as a novel self-assembling module. The motif can connect with each other through self-complementary sequences on its segments. The flexible joints can be fixed in a straightened position as desired, thereby allowing the motif to take various shapes. We can adjust the number of flexible joints and the number of connectable segments, thereby enabling programmable self-assembly of the motif. We successfully produced the motif and evaluated several self-assembly patterns. The proposed multi-joint ring motif can provide a novel method for creating functional molecular devices.

scholarly journals Auxetic Two-Dimensional Nanostructures from DNA

2020 ◽  
Author(s):  
Ruixin Li ◽  
Haorong Chen ◽  
Jong Hyun Choi
Keyword(s):  
Self Assembly ◽  
Md Simulations ◽  
Dna Origami ◽  
Coarse Grained ◽  
Two Dimensional ◽  
Materials Chemistry ◽  
Dna Nanostructures ◽  
Unit Cells ◽  
Materials Used ◽  
Architectured Materials

ABSTRACTArchitectured materials exhibit negative Poisson’s ratios and enhanced mechanical properties compared with regular materials. Their auxetic behaviors should emerge from periodic cellular structures regardless of the materials used. The majority of such metamaterials are constructed by top-down approaches and macroscopic with unit cells of microns or larger. On the other extreme, there are molecular-scale auxetics including naturally-occurring crystals which are not designable. There is a gap from few nanometers to microns, which may be filled by bottom-up biomolecular self-assembly. Here we demonstrate two-dimensional auxetic nanostructures using DNA origami. Structural reconfiguration experiments are performed by strand displacement and complemented by mechanical deformation studies using coarse-grained molecular dynamics (MD) simulations. We find that the auxetic properties of DNA nanostructures are mostly defined by geometrical designs, yet materials’ chemistry also plays an important role. From elasticity theory, we introduce a set of design principles for auxetic DNA metamaterials, which should find diverse applications.

scholarly journals Generating DNA Origami Nanostructures through Shape Annealing

2021 ◽  
Vol 11 (7) ◽  
pp. 2950
Author(s):  
Bolutito Babatunde ◽  
D. Sebastian Arias ◽  
Jonathan Cagan ◽  
Rebecca E. Taylor
Keyword(s):  
Self Assembly ◽  
Design Space ◽  
Simulated Annealing Algorithm ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Design Tools ◽  
Dna Nanostructures ◽  
Formal Approach ◽  
Annealing Algorithm ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology involves the design and self-assembly of DNA-based nanostructures. As a field, it has progressed at an exponential rate over recent years. The demand for unique DNA origami nanostructures has driven the development of design tools, but current CAD tools for structural DNA nanotechnology are limited by requiring users to fully conceptualize a design for implementation. This article introduces a novel formal approach for routing the single-stranded scaffold DNA that defines the shape of DNA origami nanostructures. This approach for automated scaffold routing broadens the design space and generates complex multilayer DNA origami designs in an optimally driven way, based on a set of constraints and desired features. This technique computes unique designs of DNA origami assemblies by utilizing shape annealing, which is an integration of shape grammars and the simulated annealing algorithm. The results presented in this article illustrate the potential of the technique to code desired features into DNA nanostructures.

Green synthesis of mixed metallic nanoparticles using room temperature self-assembly

2017 ◽  
Vol 13 (2) ◽  
pp. 4671-4677 ◽  
Author(s):  
A. M. Abdelghany ◽  
A.H. Oraby ◽  
Awatif A Hindi ◽  
Doaa M El-Nagar ◽  
Fathia S Alhakami
Keyword(s):  
Self Assembly ◽  
Metallic Nanoparticles ◽  
Room Temperature ◽  
Bimetallic Nanoparticles ◽  
Reduction Process ◽  
Nano Particles ◽  
Nano Structure ◽  
Small Shift ◽  
Molar Ratios ◽  
Vis Spectroscopy

Bimetallic nanoparticles of silver (Ag) and gold (Au) were synthesized at room temperature using Curcumin. Reduction process of silver and gold ions with different molar ratios leads to production of different nanostructures including alloys and core-shells. Produced nanoparticles were characterized simultaneously with FTIR, UV/vis. spectroscopy, transmission electron microscopy (TEM), and Energy-dispersive X-ray (EDAX). UV/vis. optical absorption spectra of as synthesized nanoparticles reveals presence of surface palsmon resonance (SPR) of both silver at (425 nm) and gold at (540 nm) with small shift and broadness of gold band after mixing with resucing and capping agent in natural extract which suggest presence of bimetallic nano structure (Au/Ag). FTIR and EDAX data approve the presence of bimetallic nano structure combined with curcumin extract. TEM micrographs shows that silver and gold can be synthesized separately in the form of nano particles using curcumin extract. Synthesis of gold nano particles in presence of silver effectively enhance and control formation of bi-metallic structure.

Fluorescence biosensor for Salmonella typhimurium detection in food based on nano-self-assembly of alendronic acid modified upconversion and gold nanoparticles

2021 ◽  
Author(s):  
Min Chen ◽  
Leiqing Pan ◽  
K. Tu
Keyword(s):  
Gold Nanoparticles ◽  
Salmonella Typhimurium ◽  
Gold Nanoparticle ◽  
Alendronic Acid ◽  
Self Assembly ◽  
Upconversion Nanoparticles ◽  
Fluorescent Biosensor ◽  
Fluorescence Biosensor

A simple and quick responsive fluorescent biosensor for Salmonella typhimurium detection based on the recognition of aptamer coupled with alendronic acid (ADA)@upconversion nanoparticles (UCNPs) and gold nanoparticle (AuNPs) has been...

scholarly journals Co-self-assembly of multiple DNA origami nanostructures in a single pot

2021 ◽  
Author(s):  
Joshua A. Johnson ◽  
Vasiliki Kolliopoulos ◽  
Carlos E. Castro
Keyword(s):  
Self Assembly ◽  
Dna Origami ◽  
Folding Pathways

We demonstrate co-self-assembly of two distinct DNA origami structures with a common scaffold strand through programmable bifurcation of folding pathways.

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