scholarly journals Characterisation of gelatin extracted from buffalo (Bubalus bubalis) bone using papain pre-treatment

2020 ◽  
Vol 1 (4) ◽  
pp. 1-5
Keyword(s):  
Emulsion Stability ◽  
Bubalus Bubalis ◽  
Raw Material ◽  
Foaming Properties ◽  
Enzymatic Extraction ◽  
Significant Difference ◽  
Foaming Capacity ◽  
Pre Treatment ◽  
Treatment Step ◽  
Potential Use

This study was carried out to extract gelatin from the bone of Buffalo (Bubalus bubalis) by incorporating enzymatic pre-treatment. Papain-aided extraction (PE) (9.1 ppm of papain at 50 °C water) was employed in the pre-treatment step, in which non-enzymatic extraction (NE) was carried out for comparison. The gelatin obtained were next evaluated for their physicochemical properties such as moisture, protein, and ash content, colour, and UV-vis absorption. Functional properties of the gelatin which included emulsifying and foaming properties were also determined. Four-fold increments in yield (wet basis) were obtained for PE (29.92 %) as compared to NE (7.5 %). Moreover, no significance difference (p > 0.05) in moisture content was observed for both PE and NE, although the protein content of the gelatin was observed in the range between 70-90 %. The resulting gelatin from both extractions was generally yellowish in appearance and was confirmed by the colourimetry data where no significant difference (p > 0.05) was observed for both samples. The maximum absorption peak for both PE and NE were observed at 210 nm, which was in the range commonly reported for gelatin. In addition, the emulsifying and foaming capacity of PE and NE had no significant difference (p > 0.05), although emulsion stability for PE was shown to be significantly higher (p < 0.05) compared to NE. The present study was an attempt to evaluate the potential use of local buffalo bone as raw material for gelatin production, which found that extractability could be improved with enzymatic pre-treatment in obtaining acceptable gelatin qualities.

scholarly journals Effects of extraction conditions on characterization of gelatin from water buffalo (Bubalus bubalis) skin

Food Research ◽  
2020 ◽  
Vol 4 (S6) ◽  
pp. 124-131
Author(s):  
Rabiatul Amirah R. ◽  
Ellya Hazreera A.J. ◽  
Nor Qhairul Izzreen M.N. ◽  
Rozzamri A. ◽  
Umi Hartina M.R.
Keyword(s):  
Water Buffalo ◽  
Extraction Process ◽  
Bubalus Bubalis ◽  
Ash Content ◽  
Raw Material ◽  
Extraction Time ◽  
Foaming Properties ◽  
Alternative Source ◽  
Significant Difference ◽  
Pre Treatment

The study aimed to determine the characteristics of gelatin from water buffalo (Bubalus bubalis) skin pre-treated with NaOH and Ca(OH)2 at different concentrations (0.3 M, 0.5 M and 0.7 M) and extracted at 65˚C for 6 hrs and 24 hrs respectively. The gelatin obtained was evaluated for its moisture, protein and ash content, UV-vis absorption value, colour, emulsifying and foaming properties. The highest yield (20.25%) was observed for gelatin extracted by 0.5 M NaOH at 24 hrs extraction time. For alkaline pre-treatment, it was found that NaOH was more efficient than Ca(OH)2 in terms of preparing the skin for subsequent extraction process. The protein content of the extracted gelatin samples was in the range of 71.76% - 87.83%, showing that the varying processing conditions are sufficiently to recover protein from the raw material. Ash content for all samples was in agreement with USDA standard, which was below than 3%. The extracted gelatin had varying pH values which were from 5.47 to 7.02. The gelatin was colourless with ‘L’ values of more than 80, except for 0.7 M Ca(OH)2 at 24 hrs which showed slightly darker properties. The intensity of the UV-vis absorption spectrum showed that a high absorption peak was observed at 6 hrs of extraction time (230 – 250 nm) compared to 24 hrs extraction time. Emulsifying properties of buffalo gelatin increased with increasing concentrations of alkaline except for 0.7 M NaOH and 0.7 M Ca(OH)2 for both extraction time. Meanwhile, foam expansion of the gelatin extracted from the different extraction conditions was observed to have a significant difference (p < 0.05) for all samples. To our knowledge, buffalo skin has the potential to be an alternative source of gelatin in the diversified industrial application by modifying the extraction conditions in order to produce gelatin with desired quality.

Evaluation of cashew nut flour in the production of biscuit

2014 ◽  
Vol 44 (3) ◽  
pp. 204-211 ◽  
Author(s):  
Ishmael Owiredu ◽  
Damian Laryea ◽  
John Barimah
Keyword(s):  
Wheat Flour ◽  
Nutrient Enrichment ◽  
Sensory Analysis ◽  
Design Methodology ◽  
Raw Material ◽  
Content Type ◽  
Overall Acceptability ◽  
Cashew Nut ◽  
Significant Difference ◽  
Potential Use

Purpose – The aim of this paper is to promote the utilization and diversification of cashew nuts through its use as a substitute for wheat flour in biscuit production. Design/methodology/approach – Wheat flour was substituted with cashew nut flour (CNF) at levels of 0, 20, 30 and 40 percent in the production of biscuit. The products obtained were subjected to proximate, mineral and sensory analysis. Findings – There was a significant increase in protein and fat contents from 7.75 and 22.11 percent to 12.89 and 32.11 percent, respectively, when CNF increased, whiles carbohydrate decreased from 66.67 to 48.04 percent. A significant increase in magnesium (27.93-97.03 mg/100 g), sodium (198.11-228.02 mg/100 g), phosphorus (55.90-149.00 mg/100 g), potassium (290.40-990.00 mg/100 g), zinc (0.72-2.00 mg/100 g) and iron (0.28-1.00 mg/100 g) was also observed as CNF increased. Sensory analysis revealed that there was no significant difference (p>0.05) between the 20 and 30 percent substituted samples, in terms of overall acceptability. Therefore, wheat flour could be substituted with CNF up to 30 percent in the production of biscuit. Originality/value – This study was done to ascertain the potential use of CNF as a substitute for wheat flour and a nutrient enrichment in biscuit production, in order to diversify its use; since it still remains an underutilized raw material in Ghana.

A novel immobilization matrix for the biosensing of phenol: self assembled monolayers of calixarenes

2017 ◽  
Vol 42 (2) ◽  
Author(s):  
Filiz Tasci ◽  
Serkan Sayin ◽  
Didem Ag Seleci ◽  
Bilal Demir ◽  
Hacer Azak ◽  
...  
Keyword(s):  
Waste Water ◽  
Industrial Waste ◽  
Self Assembled Monolayers ◽  
Industrial Waste Water ◽  
Calixarene Derivative ◽  
Assembled Monolayers ◽  
Immobilization Matrix ◽  
Pre Treatment ◽  
Treatment Step ◽  
Potential Use

AbstractAim:The development of calixarene based phenol biosensor.Methods:This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NHResults:The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was investigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step.Conclusion:The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic contaminations in samples.

The Effect of Antiplatelet Drugs on Plasma Betathromboglobulin in Coronary Artery Disease

1979 ◽  
Author(s):  
P Han ◽  
A Turpie ◽  
E Genton ◽  
M Gent
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Crossover Trial ◽  
Specific Protein ◽  
Antiplatelet Drugs ◽  
Significant Difference ◽  
Pre Treatment ◽  
Artery Disease ◽  
Therapeutic Doses ◽  
Granule Release

Platelets play a role in the development and complications of coronary artery disease (CAD) and a number of abnormalities of platelet function which can be corrected by antiplatelet drugs have been described. Betathromboglobulin (BTG), a platelet-specific protein which is released from α-granules during platelet activation is significantly elevated in patients with angiographically demonstrated CAD (51.0 ± 31.0 ng/ml., n = 50) compared to normal (28.0 ± 8.0 ng/ml., n = 70) p < 0.001. The effect of sulphinpyrazone (800 mg.) or aspirin (1200 mg.)/dipyridamole (200 mg.) on plasma BTG in CAD was studied in a blind prospective crossover trial in 25 patients. Mean BTG concentration pre-treatment was 52.3 ng/ml. and after 1 month’s treatment with placebo, sulphinpyrazone or aspirin/dipyridamole mean plasma BTG concentrations were 53.5, 49.6 and 56.7 ng/ml. respectively. Analysis of variance showed no significant difference between the means (p > 0.1) . This study confirms increased plasma BTG concentrations in patients with CAD and indicates that therapeutic doses of these antiplatelet drugs do not significantly effect the BTG level and thus appear not to prevent α-granule release in CAD.

STUDIES ON SULFATION FACTOR (SF) ACTIVITY OF HUMAN SERUM

1960 ◽  
Vol XXXV (III) ◽  
pp. 381-396 ◽  
Author(s):  
Sven Almqvist
Keyword(s):  
Human Serum ◽  
Normal Serum ◽  
Treatment Level ◽  
Response Curves ◽  
Normal Children ◽  
Significant Difference ◽  
Human Sera ◽  
Normal Humans ◽  
Pre Treatment ◽  
Dose Levels

ABSTRACT The sulfation factor (SF) activity of human sera has been estimated using a modification of the method of Daughaday et al. (1959). Each assay was statistically evaluated. The method had a mean precision of 0.14 and, used as an assay of GH of human serum, a sensitivity in three pituitary dwarfs of 0.1 to 0.6 μg of HGH/ml of serum. SF activity was found at all ages between 1 month and 75 years. There was a significantly lower mean SF activity below the age of half a year. Three cases of pituitary dwarfism had significantly low SF activities of sera. There was no significant difference between the SF activities of sera from untreated pituitary dwarfs and the sera from normal children below half a year of age. Dose-response curves with large volumes of sera from pituitary dwarfs and small volumes of sera from normal humans had the same slopes. Four mg of HGH prepared according to the method of Li & Papkoff (1956) resulted in a normal serum SF activity in each of the three dwarfs. A significant (P < 0.01) linear relationship was found between the concentration of SF activity of sera from these subjects and the logarithm of the dose of HGH given with dose levels of 1, 2 and 4 mg daily for three days. The decline of serum SF activity to the pre-treatment level following HGH in one dwarf suggested a half life not different from that indicated by others for growth hormone.

Perbandingan Karakteristik Surfaktan Metil Ester Sulfonat dan Sodium Lauril Sulfonat sebagai Bahan Emulsifier

2016 ◽  
Vol 9 (2) ◽  
pp. 167-176
Author(s):  
Eldha Sampepana ◽  
Paluphy Eka Yustini ◽  
Aditya Rinaldi ◽  
Amiroh Amiroh
Keyword(s):  
Fatty Acids ◽  
Surface Tension ◽  
Methyl Ester ◽  
Interfacial Tension ◽  
Palm Oil ◽  
Emulsion Stability ◽  
Raw Material ◽  
Enzymatic Method

Surfactant which is used as raw emulsifier in an industry activity such as Sodium Lauryl Sulfonate is a raw material import, it is petroleum derivative which is not renewable and may cause pollution to the environment, because it is not degraded and are carcinogenic. The purpose of the research is to compare the characteristics of the Quaternary methyl ester sulfonat (MES) and Sodium Lauryl Sulfonat (SLS) as emulsifier. First, make the MES by filtering and eliminating fatty acids of palm oil, then process the MES with enzymatic method become methyl ester, then react it in sulfonation and metanolization process, and also neutralized with NaOH. Next, the MES experiment is compared with SLS and existing MES in the market. The results show that surfactants MES experiment has value hidrofil lipofil balance (HLB) interfacial tension and emulsion stability greater than MES in the market and SLS. And the surface tension of MES experiment is larger than MES in the market, but smaller compared to SLS.ABSTRAKSurfaktan yang digunakan sebagai bahan baku emulsifer dalam aktivitas suatu industri pada saat ini seperti Sodium Lauril Sulfonat  merupakan bahan baku import yang merupakan turunan dari minyak bumi, dengan sifat tidak dapat diperbaharui dan dapat menimbulkan pencemaran terhadap lingkungan karena tidak mudah terdegradasi serta bersifat karsinogenik. Metil ester sulfonat dari bahan minyak sawit merupakan surfaktan dengan sifat mudah terdegradasi yang perlu diketahui karakteristiknya. Penelitian bertujuan untuk membandingkan karakteristik surfaktan metil ester sulfonat (MES) dan Sodium Lauril Sulfonat (SLS) sebagai bahan emulsifier. Mula-mula dilakukan pembuatan MES dengan cara menyaring dan menghilangkan asam lemak minyak sawit terlebih dahulu, kemudian diolah menjadi metil ester secara enzimatis, lalu direaksikan secara sulfonasi dan metanolisis, serta dinetralkan dengan NaOH. Selanjutnya MES hasil percobaan dibandingkan dengan SLS dan MES yang ada dipasaran. Hasil penelitian menunjukkan bahwa surfaktan MES memiliki nilai hidrofil lipofil balance (HLB) tegangan antar muka dan stabilitas emulsi lebih besar apabila dibandingkan dengan MES di pasaran dan SLS, kecuali nilai stabilitas emulsi antara MES dan SLS sama. Dan tegangan permukaan MES hasil percobaan, lebih besar dibandingkan dengan MES dipasaran, dan lebih kecil dibandingkan dengan SLS. Kata kunci :   Metil  ester sulfonat, hidrofil lipofil balance, emulsifier, sodium lauril sulfonat , stabilitas emulsi 

Isolation of an Anti-fatigue Preparation from Cellulase-treated Maca by Hydroalcoholic Extraction

2017 ◽  
Vol 17 (1) ◽  
pp. 93-98
Author(s):  
Zheng Yue ◽  
Zhang Wen-Cheng ◽  
Wu Ze-Yu ◽  
Fu Chuan-Xiang ◽  
Gao Han ◽  
...  
Keyword(s):  
Positive Control ◽  
Glycogen Storage ◽  
Hepatic Glycogen ◽  
Time To Exhaustion ◽  
High Dose ◽  
Hydroalcoholic Extract ◽  
Swimming Time ◽  
Significant Difference ◽  
Pre Treatment ◽  
Better Than

The purpose of this study was to evaluate the anti-fatigue activity of maca hydroalcoholic extract (ME), which mainly contains macamides and polysaccharides. ME was prepared by circumfluence extraction with enzymatic pre-treatment. Anti-fatigue activity of ME was investigated in weight-loaded forced swimming mice, with pure macamides and commercially available maca tablet as positive control. Compared with normal group, pure macamides treatment group could prolong the swimming time to exhaustion, but there was no statistically significant difference (P > 0.05); while ME (middle-dose and high-dose groups) could effectively prolong the swimming durations (P < 0.05). Supplementation with pure macamides significantly decreased blood lactic acid (BLA), whereas ME significantly increased hepatic glycogen (HG), decreased BLA, and blood urea nitrogen (BUN) compared with those in normal control (P < 0.05). The results suggested that the anti-fatigue effect of ME was better than that of pure macamides, which can be explained by the increase of glycogen storage and the reduction of metabolites accumulation.

scholarly journals Organ motion in linac-based SBRT for glottic cancer

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Annarita Perillo ◽  
Valeria Landoni ◽  
Alessia Farneti ◽  
Giuseppe Sanguineti
Keyword(s):  
Early Stage ◽  
Displacement Vector ◽  
Thyroid Cartilage ◽  
Three Dimensional ◽  
Target Volume ◽  
Glottic Cancer ◽  
Organ Motion ◽  
Delivery Times ◽  
Significant Difference ◽  
Pre Treatment

Abstract Purpose The purpose of this study is to evaluate inter- and intra-fraction organ motion as well as to quantify clinical target volume (CTV) to planning target volume (PTV) margins to be adopted in the stereotactic treatment of early stage glottic cancer. Methods and materials Stereotactic body radiotherapy (SBRT) to 36 Gy in 3 fractions was administered to 23 patients with early glottic cancer T1N0M0. Patients were irradiated with a volumetric intensity modulated arc technique delivered with 6 MV FFF energy. Each patient underwent a pre-treatment cone beam computed tomography (CBCT) to correct the setup based on the thyroid cartilage position. Imaging was repeated if displacement exceeded 2 mm in any direction. CBCT imaging was also performed after each treatment arc as well as at the end of the delivery. Swallowing was allowed only during the beam-off time between arcs. CBCT images were reviewed to evaluate inter- and intra-fraction organ motion. The relationships between selected treatment characteristics, both beam-on and delivery times as well as organ motion were investigated. Results For the population systematic (Ʃ) and random (σ) inter-fraction errors were 0.9, 1.3 and 0.6 mm and 1.1, 1.3 and 0.7 mm in the left-right (X), cranio-caudal (Y) and antero-posterior (Z) directions, respectively. From the analysis of CBCT images acquired after treatment, systematic (Ʃ) and random (σ) intra-fraction errors resulted 0.7, 1.6 and 0.7 mm and 1.0, 1.5 and 0.6 mm in the X, Y and Z directions, respectively. Margins calculated from the intra-fraction errors were 2.4, 5.1 and 2.2 mm in the X, Y and Z directions respectively. A statistically significant difference was found for the displacement in the Z direction between patients irradiated with > 2 arcs versus ≤ 2 arcs, (MW test, p = 0.038). When analyzing mean data from CBCT images for the whole treatment, a significant correlation was found between the time of delivery and the three dimensional displacement vector (r = 0.489, p = 0.055), the displacement in the Y direction (r = 0.553, p = 0.026) and the subsequent margins to be adopted (r = 0.626, p = 0.009). Finally, displacements and the subsequent margins to be adopted in Y direction were significantly greater for treatments with more than 2 arcs (MW test p = 0.037 and p = 0.019, respectively). Conclusions In the setting of controlled swallowing during treatment delivery, intra-fraction motion still needs to be taken into account when planning with estimated CTV to PTV margins of 3, 5 and 3 mm in the X, Y and Z directions, respectively. Selected treatments may require additional margins.

Bark characterization of a commercial Eucalyptus urophylla hybrid clone in view of its potential use as a biorefinery raw material

2021 ◽  
Author(s):  
Caroline J. Sartori ◽  
Graciene S. Mota ◽  
Fábio Akira Mori ◽  
Isabel Miranda ◽  
Teresa Quilhó ◽  
...  
Keyword(s):  
Raw Material ◽  
Hybrid Clone ◽  
Eucalyptus Urophylla ◽  
Potential Use

scholarly journals Comparison between Pressurized Liquid Extraction and Conventional Soxhlet Extraction for Rosemary Antioxidants, Yield, Composition, and Environmental Footprint

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 584 ◽  
Author(s):  
Mathilde Hirondart ◽  
Natacha Rombaut ◽  
Anne Sylvie Fabiano-Tixier ◽  
Antoine Bily ◽  
Farid Chemat
Keyword(s):  
Extraction Procedure ◽  
Soxhlet Extraction ◽  
Liquid Extraction ◽  
Raw Material ◽  
Pressurized Liquid Extraction ◽  
Carnosic Acid ◽  
Environmental Footprint ◽  
Initial Composition ◽  
Significant Difference ◽  
Preparation Step

Nowadays, “green analytical chemistry” challenges are to develop techniques which reduce the environmental impact not only in term of analysis but also in the sample preparation step. Within this objective, pressurized liquid extraction (PLE) was investigated to determine the initial composition of key antioxidants contained in rosemary leaves: Rosmarinic acid (RA), carnosic acid (CA), and carnosol (CO). An experimental design was applied to identify an optimized PLE set of extraction parameters: A temperature of 183 °C, a pressure of 130 bar, and an extraction duration of 3 min enabled recovering rosemary antioxidants. PLE was further compared to conventional Soxhlet extraction (CSE) in term of global processing time, energy used, solvent recovery, raw material used, accuracy, reproducibility, and robustness to extract quantitatively RA, CA, and CO from rosemary leaves. A statistical comparison of the two extraction procedure (PLE and CSE) was achieved and showed no significant difference between the two procedures in terms of RA, CA, and CO extraction. To complete the study showing that the use of PLE is an advantageous alternative to CSE, the eco-footprint of the PLE process was evaluated. Results demonstrate that it is a rapid, clean, and environmentally friendly extraction technique.

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