High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

AbstractThe vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions.

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Design of Low-Cost and High-Strength Titanium Alloys Using Pseudo-Spinodal Mechanism through Diffusion Couple Technology and CALPHAD

Materials ◽

10.3390/ma14112910 ◽

2021 ◽

Vol 14 (11) ◽

pp. 2910

Author(s):

Chaoyi Ding ◽

Chun Liu ◽

Ligang Zhang ◽

Di Wu ◽

Libin Liu

Keyword(s):

Diffusion Couple ◽

Titanium Alloys ◽

High Throughput ◽

Volume Fraction ◽

Raw Materials ◽

Low Cost ◽

High Strength ◽

High Yield ◽

Α Phase ◽

Cr System

The high cost of development and raw materials have been obstacles to the widespread use of titanium alloys. In the present study, the high-throughput experimental method of diffusion couple combined with CALPHAD calculation was used to design and prepare the low-cost and high-strength Ti-Al-Cr system titanium alloy. The results showed that ultra-fine α phase was obtained in Ti-6Al-10.9Cr alloy designed through the pseudo-spinodal mechanism, and it has a high yield strength of 1437 ± 7 MPa. Furthermore, application of the 3D strength model of Ti-6Al-xCr alloy showed that the strength of the alloy depended on the volume fraction and thickness of the α phase. The large number of α/β interfaces produced by ultra-fine α phase greatly improved the strength of the alloy but limited its ductility. Thus, we have demonstrated that the pseudo-spinodal mechanism combined with high-throughput diffusion couple technology and CALPHAD was an efficient method to design low-cost and high-strength titanium alloys.

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Efficient High-throughput Techniques for the Analysis of Disease-Resistant Plant Varieties and Detection of Food Adulteration

Current Protein and Peptide Science ◽

10.2174/1389203723666211223111238 ◽

2021 ◽

Vol 23 ◽

Author(s):

Romesh Kumar Salgotra ◽

Rafiq Ahmad Bhat ◽

Deyue Yu ◽

Javaid Akhter Bhat

Keyword(s):

Multiplex Pcr ◽

High Throughput ◽

High Throughput Sequencing ◽

Low Cost ◽

Crop Improvement ◽

Small Sample ◽

Food Crops ◽

Genomic Resources ◽

Breeding Programmes ◽

Next Generation Sequencing Ngs

Abstract: Over the past two decades, the advances in the next generation sequencing (NGS) platforms have led to the identification of numerous genes/QTLs at high-resolution for their potential use in crop improvement. The genomic resources generated through these high-throughput sequencing techniques have been efficiently used in screening of particular gene of interest particularly for numerous types of plant stresses and quality traits. Subsequently, the identified-markers linked to a particular trait have been used in marker-assisted backcross breeding (MABB) activities. Besides, these markers are also being used to catalogue the food crops for detection of adulteration to improve the quality of food. With the advancement of technologies, the genomic resources are originating with new markers; however, to use these markers efficiently in crop breeding, high-throughput techniques (HTT) such as multiplex PCR and capillary electrophoresis (CE) can be exploited. Robustness, ease of operation, good reproducibility and low cost are the main advantages of multiplex PCR and CE. The CE is capable of separating and characterizing proteins with simplicity, speed and small sample requirements. Keeping in view the availability of vast data generated through NGS techniques and development of numerous markers, there is a need to use these resources efficiently in crop improvement programmes. In summary, this review describes the use of molecular markers in the screening of resistance genes in breeding programmes and detection of adulterations in food crops using high-throughput techniques.

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High Throughput and Low-Cost Detection of Short Nucleic Acid Biomarkers in Serum using Dielectrophoretic Biosensor

2018 IEEE Research and Applications of Photonics In Defense Conference (RAPID) ◽

10.1109/rapid.2018.8508984 ◽

2018 ◽

Cited By ~ 2

Author(s):

Logeeshan Velmanickam ◽

Ivan T. Lima ◽

Dharmakeerthi Nawarathna

Keyword(s):

Nucleic Acid ◽

High Throughput ◽

Low Cost

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MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

SLAS DISCOVERY Advancing Life Sciences ◽

10.1177/2472555217727517 ◽

2017 ◽

Vol 22 (10) ◽

pp. 1246-1252 ◽

Cited By ~ 1

Author(s):

Kishore Kumar Jagadeesan ◽

Simon Ekström

Keyword(s):

Mass Spectrometry ◽

High Throughput ◽

High Throughput Screening ◽

Web Application ◽

Low Cost ◽

Maldi Ms ◽

Ionization Mass ◽

Label Free ◽

Label Free Detection ◽

R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

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Computational Method for Simulating Thermoset Polymer Curing and Prediction of Thermophysical Properties

10.26434/chemrxiv.12453989.v1 ◽

2020 ◽

Author(s):

Jeffrey Sanders ◽

Carla E. Estridge ◽

Matthew B Jackson ◽

Thomas JL Mustard ◽

Samuel J. Tucker ◽

...

Keyword(s):

High Throughput ◽

Thermophysical Properties ◽

High Throughput Screening ◽

Simulation Analysis ◽

Low Cost ◽

Polymer Network ◽

Thermomechanical Properties ◽

Computational Method ◽

Cross Linking ◽

Automated Simulation

Thermoset polymers are an area of intense research due to their low cost, ease of processing, environmental resistance, and unique physical properties. The favorable properties of this class of polymers have many applications in aerospace, automotive, marine, and sports equipment industries. Molecular simulations of thermosets are frequently used to model formation of the polymer network, and to predict the thermomechanical properties. These simulations usually require custom algorithms that are not easily accessible to non-experts and not suited for high throughput screening. To address these issues, we have developed a robust cross-linking algorithm that can incorporate different types of chemistries and leverage GPU-enabled molecular dynamics simulations. Automated simulation analysis tools for cross-linking simulations are also presented. Using four well known epoxy/amine formulations as a foundational case study and benzoxazine as an example of how additional chemistries can be modeled, we demonstrate the power of the algorithm to accurately predict curing and thermophysical properties. These tools are able to streamline the thermoset simulation process, opening up avenues to in-silico high throughput screening for advanced material development.

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Computational High-Throughput Screening of Polymeric Photocatalysts: Exploring the Effect of Composition, Sequence Isomerism and Conformational Degrees of Freedom

10.26434/chemrxiv.7314929.v3 ◽

2018 ◽

Author(s):

isabelle Heath-Apostolopoulos ◽

Liam Wilbraham ◽

Martijn Zwijnenburg

Keyword(s):

High Throughput ◽

High Throughput Screening ◽

Degrees Of Freedom ◽

Chemical Space ◽

Low Cost ◽

Computational Screening ◽

Computational Workflow ◽

Conformational Degrees Of Freedom

We discuss a low-cost computational workflow for the high-throughput screening of polymeric photocatalysts and demonstrate its utility by applying it to a number of challenging problems that would be difficult to tackle otherwise. Specifically we show how having access to a low-cost method allows one to screen a vast chemical space, as well as to probe the effects of conformational degrees of freedom and sequence isomerism. Finally, we discuss both the opportunities of computational screening in the search for polymer photocatalysts, as well as the biggest challenges.

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A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System

Biomolecules ◽

10.3390/biom10040517 ◽

2020 ◽

Vol 10 (4) ◽

pp. 517

Author(s):

Seohee Chang ◽

Soohyun Kim ◽

Jerome Han ◽

Suji Ha ◽

Hyunho Lee ◽

...

Keyword(s):

High Throughput ◽

Retrieval System ◽

Low Cost ◽

Therapeutic Antibodies ◽

Amino Acid Residues ◽

Single Chain ◽

Scfv Library ◽

Single Clone ◽

Library Complexity ◽

Lower Complexity

Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 105 harboring random mutations at five amino acid residues, more than 70,000 clones—corresponding to ~14% of the library complexity—were screened, resulting in 78 antigen-reactive scFv sequences with mutations restricted to the randomized residues. Our results demonstrate that this system can significantly reduce the number of biopanning rounds, or even eliminate the need for this process for libraries with lower complexity, providing an opportunity to obtain more diverse clones from the library.

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Time Slicing Method

Multimedia Information Storage and Retrieval ◽

10.4018/978-1-59904-225-1.ch018 ◽

2011 ◽

pp. 272-279

Author(s):

Phillip K.C. Tse

Keyword(s):

High Throughput ◽

Storage Capacity ◽

Low Cost ◽

Storage Devices ◽

Access Latency ◽

Tertiary Storage ◽

Start Up ◽

Slicing Method ◽

Time Slicing

Tertiary storage devices provide huge storage capacity at low cost. Multimedia objects stored on the tertiary storage devices are accessed with high latency. Despite the high access latency, some tertiary storage devices are able to deliver data at high throughput. The time slicing method is designed to reduce the start-up latency in accessing multimedia objects from tertiary storage devices. The start-up latency is lowered by reducing the amount of data being migrated in stage one of the staging method being described in the last chapter. In order to support the time-slicing method, the tertiary storage devices should have the ability to deliver data at high throughput. The tertiary storage devices that cannot deliver data at sufficiently high throughput; the start-up latency cannot be reduced.

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