The Effects of Autologous Platelet-Rich Plasma on the Bone Fracture Healing in Rabbits

The purpose of this study was to evaluate the effects of an autologous platelet rich-plasma on femoral fracture healing in experimentally induced rabbits’ model. In this study, 34 local breed rabbits, around 2-2.5 kg body weight, were divided into two equal groups randomly; Group A: platelet-rich plasma (PRP) and Groups B: Control group. Rabbits in both groups underwent a complete mid-shaft transverse osteotomy of the femur by a fine electrical saw. After fracture induction, the rabbits in group A (PRP group) were treated by application of 0.5 ml of autologous PRP at the site of the fractured bone, while in group B (control), rabbits were injected with distilled water. Samples from the fractured femur were collected at 10th, 20th, 30th days post operation for radiological evaluation and at 10th, 14th, 20th, and 30th days for histopathological evaluation. In radiological study, it was found that the rate of callus formation in rabbits treated with PRP (Group A) were faster than the control group (Group B) at different periods. Similarly, in histological finding it found that the stages of healing were faster in Group A when compared with Group B. It was concluded that using autologous PRP has beneficial effect to enhance the process of bone healing in the rabbit’s model.

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A comparative study between local injection of autologous platelet rich plasma and injection of corticosteroid in functional improvement of plantar fasciitis

International Surgery Journal ◽

10.18203/2349-2902.isj20190809 ◽

2019 ◽

Vol 6 (3) ◽

pp. 653 ◽

Cited By ~ 1

Author(s):

Vithal Prakash Puri ◽

Anil Kumar Gaur

Keyword(s):

Plantar Fasciitis ◽

Platelet Rich Plasma ◽

Tertiary Care ◽

Functional Improvement ◽

Significant Difference ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Background: Plantar fasciitis is a common cause of heel pain in adults. Although it is usually a self-limiting condition, the pain may become prolonged and severe enough to cause significant distress and disruption to the patient’s daily activities and work. The primary objective of the study was to evaluate and compare the effectiveness of autologous platelet rich plasma (PRP) and steroid injections in chronic cases of plantar fasciitis (PF).Methods: A prospective, randomized study was conducted from December 2013 to December 2015 amongst 60 patients with chronic PF were randomized prospectively in single tertiary care center in India. All the patients were enrolled according to inclusion criteria and divided into 2 groups i.e. group A (n=30) received PRP and group B (n=30) received corticosteroids injections. Roles and Maudsley score (RM Score) and Foot Function Index (FFI) was evaluated for all the included patients. The follow-up scheduled at 1 and 6 months after complete enrolment of patients.Results: Between both the groups, the significant difference was observed at 1 and 6 months follow-up from the baseline. At 1-month follow-up, statistically significant improvement in mean RM scores were seen in both the groups from baseline and when RM scores were compared between two groups, group B had statistically better mean scores. At 1-month follow-up there was no statistically significant difference between the mean FFI score values between two groups. At 6-month follow-up, statistically significant improvement in mean FFI scores were seen in both the groups, however when both groups were compared to each other, improvement in mean FFI scores was statistically better in group A as compared to group B.Conclusions: The present study concluded the use of PRP in chronic cases of plantar fasciitis seems more safe and effective in long term than the traditional treatment of steroid injection at different time period.

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P–301 Intrauterine instillation of autologous platelet rich plasma for thin endometrium improves the outcome of frozen embryo transfer cycles

Human Reproduction ◽

10.1093/humrep/deab130.300 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

S Nagireddy ◽

S R Nellepalli ◽

R Vembu ◽

M Pandurangi ◽

M Gopal. Krishnan ◽

...

Keyword(s):

Embryo Transfer ◽

Live Birth ◽

Endometrial Thickness ◽

Platelet Rich Plasma ◽

Estradiol Valerate ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Study Participants ◽

Autologous Platelet

Abstract Study question How does the intrauterine instillation of autologous platelet rich plasma (PRP) affect the endometrial thickness and live birth rate in frozen embryo transfer cycles? Summary answer Intrauterine instillation of autologous PRP resulted in significant improvement in endometrial thickness. The live birth rates were satisfactory post-PRP instillation. What is known already Autologous Platelet rich plasma (PRP) had resulted in significant improvement in endometrial thickness, when instilled intrauterine in women with thin endometrium in FET cycles. Study design, size, duration A retrospective observational study was performed at a tertiary care university teaching hospital in South India. 35 women who received intrauterine autologous PRP during endometrial preparation for frozen embryo transfer from June 2017 to December 2020, were included. Patients who underwent donor oocyte recipent cycles, those with a history of tubercular endometritis, Asherman syndrome, previous intrauterine manipulations such as manual removal of placenta, and uterine anomalies were excluded. Participants/materials, setting, methods All the women underwent endometrial preparation in artificial cycles by depot GnRH agonist suppression and HRT (Hormone replacement therapy) was initiated by 4–6 mg of estradiol valerate and stepped up as required. Autologous PRP was offered to all women who had endometrial thickness < 7 mm on day 16 of HRT. PRP was prepared by the two-step centrifugation method and administered intrauterine by IUI catheter. The patients underwent repeat evaluation after 5 days post-PRP instillation. Main results and the role of chance Optimal response to PRP was considered as the attainment of an endometrial thickness (ET) ≥ 7mm after 5 days of post-PRP. 25 (71.4%) had an optimal response to PRP. There was a significant improvement in the endometrial thickness(mm) in the study participants following PRP instillation: 6.3 ± 0.6 vs. 7.1 ± 1.2; P = 0.0001.The study participants were divided into two groups based on their response to intrauterine PRP instillation. Those who optimally responded to PRP were categorized as Group A and those who didn’t were categorized as Group B. The study participants of both the groups were comparable by their demographic characteristics such as age, cause of infertility, indications for ART, and the dose of estradiol valerate before PRP. The dose of estradiol valerate (mg) after PRP was significantly higher in Group B compared to Group A: 19.9 ± 4.9 vs. 15.6 ± 3.9; P = 0.014. A total of 26 women underwent embryo transfer and 9 (25.7%) women had cycle cancellation. Of these 22 were from Group A and 4 from Group B. The pregnancy, clinical pregnancy, miscarriage and live birth rates were 36.3%(8/22) and 25% (1/4); 31.8% (7/22) and 25% (1/4); and 31.8% (7/22) and 25% (1/4), respectively. Limitations, reasons for caution As the study was retrospective in nature and the PRP was offered only in patients who had consented, there was a significant bias. Hence the results of the study should be interpreted with caution. Further large prospective RCTs (Randomised controlled trials) are required to confirm our findings. Wider implications of the findings: Autologous PRP may enhance the response to the estrogen preparations. It may produce satisfactory live birth rates and reduce cycle cancellations in a reasonable proportion of patients with thin endometrium in FET cycles. However, these findings should be confirmed by dose finding clinical trials, and studies involving a comparison group. Trial registration number Not applicable

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The comparative and prospective study on efficacy and functional outcome of autologous platelet rich plasma injection vs hydrodissection in adhesive capsulitis of shoulder

International Journal of Research in Orthopaedics ◽

10.18203/issn.2455-4510.intjresorthop20184190 ◽

2018 ◽

Vol 4 (6) ◽

pp. 848 ◽

Cited By ~ 1

Author(s):

Madhan Jeyaraman ◽

Ramesh R. ◽

Prajwal G. S. ◽

Hardik J. Dhamsania

Keyword(s):

Quality Of Life ◽

Functional Outcome ◽

Platelet Rich Plasma ◽

Adhesive Capsulitis ◽

Dash Score ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Background: Adhesive capsulitis of should is also called frozen shoulder which describes a chronic, indolent pathological process in which the body forms excessive adhesions across the glenohumeral joint which in turn leads to pain, stiffness, and loss of range of movements which compromises the quality of life. The objective of the study was to evaluate the efficacy and functional outcome of autologous PRP injection and hydrodissection in adhesive capsulitis of shoulder.Methods: After excluding the patients who failed to satisfy the study protocol, the remaining 100 patients are divided equally into two groups namely group A (n=50) who receive autologous PRP injection and group B (n=50) who receive hydrodissection for adhesive capsulitis of shoulder. Both group participants are followed up pre-procedurally and post-procedurally at the end of 1st, 6th and 12th month for pain relief and range of movements. The improvements in pain and range of movements are charted in terms of VAS and DASH scoring system.Results: The statistical analysis were done for 46 patients in group A and 45 patients in group B which showed a statistical improvement in pain and range of movements (p<0.001 for VAS score and p<0.01 for DASH score) in group A who received autologous platelet rich plasma therapy. Autologous PRP therapy improves the functional quality of life with a long term outcome.Conclusions: For adhesive capsulitis of shoulder, autologous PRP therapy remains functionally superior than hydrodissection as autologous PRP is a constructive procedure by rejuvenating the degenerative tissues.

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The Effect of Autologous Platelet Rich Plasma in the Treatment of Achilles Tendon Ruptures

Orthopaedic Journal of Sports Medicine ◽

10.1177/2325967114s00124 ◽

2014 ◽

Vol 2 (11_suppl3) ◽

pp. 2325967114S0012

Author(s):

Baran Şen ◽

Serkan Güler ◽

Berivan Çeçen ◽

Erdem Kumtepe ◽

Alper Bağrıyanık ◽

...

Keyword(s):

Achilles Tendon ◽

Platelet Rich Plasma ◽

Healing Process ◽

Achilles Tendon Rupture ◽

Post Surgery ◽

Group A ◽

Tendon Ruptures ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Objectives: The aim of this study was to determine the effect of autologous platelet rich plasma (PRP) in the treatment of Achilles tendon ruptures in rabbits. Methods: The study included 14 New Zealand albino rabbits that were divided randomly into 2 groups, A and B, each containing seven rabbits. On day zero, all 28 Achilles tendons were tenotomized and repaired. In group A, the tendons were injected with PRP post surgery whereas those in group B were left untreated. On day 28, the right tendons in both groups were examined histopathologically via both light and electron microscopy, and the left tendons were subjected to biomechanical testing. Results: The histological and biomechanical findings in group A were better than those in group B, but the difference was not significant. Conclusion: PRP had no effect on the healing process 28 days post Achilles tendon rupture.

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A Comparison of the Autologous Platelet-Rich Plasma Gel Fat Graft Myringoplasty and the Fat Graft Myringoplasty for the Closure of Different Sizes of Tympanic Membrane Perforations

Ear Nose & Throat Journal ◽

10.1177/0145561319900388 ◽

2020 ◽

Vol 99 (5) ◽

pp. 331-336 ◽

Cited By ~ 3

Author(s):

Tolga Ersözlü ◽

Erdogan Gultekin

Keyword(s):

Success Rate ◽

Tympanic Membrane ◽

Platelet Rich Plasma ◽

Chronic Otitis Media ◽

Fat Graft ◽

Tympanic Membrane Perforations ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Objective: We aimed to evaluate the effect of autologous platelet-rich plasma gel (PRPG) on fat graft myringoplasty (FGM) in tympanic membrane perforations caused by chronic otitis media. Methods: This retrospective study involved 63 patients who underwent operations between 2015 and 2018. Fat graft myringoplasty was performed with the transcanal approach with and without the use of PRPG in the surgical field. The patients were classified into 2 groups: group A, which included 32 patients who underwent FGM with the use of autologous PRPG, and group B, which included 31 patients who underwent FGM alone. Tympanic membrane perforations were divided into 2 groups: small perforations (1-2 mm) in a single quadrant and large perforations (2-4 mm) in at least 2 quadrants. Results: Both groups were statistically matched regarding age and sex. The mean postoperative follow-up was 11.6 and 12.1 months for groups A and B, respectively. Four months postoperatively, the success rate of the graft in group A (100%) was significantly higher than that in group B (83.8%; P = .03). The preoperative and postoperative median air–bone gaps of the groups were similar ( P = .653 and P = .198, respectively). No worsening of the air–bone gap was noted postoperatively in either group. Conclusions: This study demonstrated that autologous PRPG application during FGM allows for a higher success rate than FGM alone. Furthermore, the use of PRPG with FGM for large perforations increases the success rate. Further studies are needed to investigate the PRPG healing effect on other tympanic membrane perforation closure techniques.

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Outcome of staged injection of autologous platelet rich plasma in treatment of mild to moderate knee osteoarthritis

International Journal of Research in Orthopaedics ◽

10.18203/issn.2455-4510.intjresorthop20195160 ◽

2019 ◽

Vol 6 (1) ◽

pp. 26

Author(s):

Rajat Gupta ◽

Ram Avtar ◽

Krishan Kumar

Keyword(s):

Growth Factors ◽

Single Injection ◽

Platelet Rich Plasma ◽

Low Cost ◽

Biologically Active ◽

Plasma Injection ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Background: Autologous platelet-rich plasma (PRP) offers an easy solution for delivering multiple growth factors needed for tissue repair. Intra-articular injections of PRP have been proposed as a simple low cost minimally invasive way to obtain the concentration of growth factors and biologically active molecules to promote cartilage healing in osteoarthritic (OA) knee joint. The objective of the present study was designed to evaluate the clinical efficacy of autologous platelet rich plasma injection in mild to moderate osteoarthritic knee and to assess the role of serial staged autologous platelet rich plasma injection.Methods: 50 patients with mild to moderate osteoarthritis of knee were divided into two groups. Group A was given staged injection of freshly prepared autologous PRP in the affected knee. Group B was given single injection of PRP. The Results were evaluated on the basis of the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire and visual analog scale (VAS) before treatment and 3 weeks, 8 weeks, 16 weeks and 24 weeks after treatment.Results: At 24-weeks follow up the following observations were made; the MeanVAS in Group A decreased to 3.30 whereas in Group B it increased to 4.54. The Mean WOMAC score in Group A was reduced to a mere28.64 in Group A and in Group B it was 39.76.Conclusions: It is concluded from our study that concomitant use of serial staged injection (two) of PRP over four months is more effective than single injection in patients with mild to moderate OA of knee.

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Efficacy of intralesional corticosteroid versus autologous platelet rich plasma injection in the management of chronic plantar fasciitis

International Journal of Research in Orthopaedics ◽

10.18203/issn.2455-4510.intjresorthop20183683 ◽

2018 ◽

Vol 4 (5) ◽

pp. 790

Author(s):

Divya Anil Kumar ◽

Harsha Kumar Koramutla

Keyword(s):

Plantar Fasciitis ◽

Platelet Rich Plasma ◽

Vas Score ◽

Plasma Injection ◽

Intralesional Corticosteroid ◽

Group A ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Background: Plantar fasciitis is a common pathological condition affecting the hind foot, and a common cause of heel pain. The present study was taken up to assess the efficacy of intralesional corticosteroid compared to autologous platelet rich plasma injection in the management of chronic plantar fasciitis.Methods: Patients were randomized into two groups (Group A and Group B) of 30 each. Group-A received Corticosteroid injection while Group-B received PRP injection. Patients were assessed functionally using American Orthopaedic Foot and ankle score (AOFAS), Visual analogue scale (VAS) scores before treatment and on follow-up visit at 6 weeks, 3rd month, and 6th month. Ultrasound of heel for plantar fascia thickness was measured before treatment and follow up visit at 6th month.Results: A significant decrease in VAS score was observed in the corticosteroid group compared to PRP group at 6 weeks while the VAS score continued to decrease in the PRP group at 3 months and 6 months with an increase in the corticosteroid group at 3 months and 6 months. A significant increase in AOFAS was observed in the Corticosteroid group compared to PRP group at 6 weeks which increased in the PRP group at 3 months and 6 months. However it decreased in the corticosteroid group at 3 months and 6 months. Ultrasonographic evaluation showed improvement in fascial thickness in both the groups, but was better in the PRP group.Conclusions: To conclude our study shows that corticosteroid is more effective for short term relief and PRP is more effective for long term relief.

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Comparison of efficacy of topical 5% minoxidil alone versus topical 5% minoxidil with autologous Platelet Rich Plasma (PRP) therapy in treatment of androgenetic alopecia: A randomised open label study

IP Indian Journal of Clinical and Experimental Dermatology ◽

10.18231/j.ijced.2021.021 ◽

2021 ◽

Vol 7 (2) ◽

pp. 107-114

Author(s):

Bela Padhiar ◽

Sruthy Raveendran

Keyword(s):

Platelet Rich Plasma ◽

Androgenetic Alopecia ◽

Androgenic Alopecia ◽

Open Label ◽

Hair Density ◽

Convenience Sample ◽

Double Spin ◽

Autologous Platelet Rich Plasma ◽

Group B ◽

Autologous Platelet

Androgenic alopecia (AGA) is a type of progressive hairloss, where there is androgen mediated conversion of susceptible terminal hairs into vellus hairs, in genetically predisposed individuals. To compare efficacy of Topical 5% Minoxidil alone versus Topical 5% Minoxidil with Autologous Platelet Rich Plasma (PRP) therapy in patients with Androgenetic Alopecia.This is aProspective study conducted in Department of Dermatology GMERS Medical College, Gandhinagar, Gujarat. A convenience sample of 62 men in the age group of 20-40 with Grade 2-5 AGA according to Hamilton- Norwood Grading were selected and was divided into 2 groups of 31 each. Presitting digital photographs and dermoscopic photos were taken. Autologous PRP was prepared using 18 ml of patients blood after double spin centrifugation and injected by Nappage technique. Results were assessed at the baseline and at the end of each sitting on the basis of change in hair density, photographic evaluation and patient’s self satisfaction. Highly significant increase in hair density was achieved after 4 months of treatment. At T4 (Fourth Session of treatment) Group B showed higher hair density (42.97± 8.96) as compared to Group A (36.94 ± 11.57) which was statistically significant at P = 0.03Group B showed better improvement as compared to Group A.PRP treatment has a positive therapeutic effect on male Androgenetic alopecia without major side effects.

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Efficacy of autologous platelet-rich plasma on graft uptake in myringoplasty: a single-blinded randomized control trial

The Egyptian Journal of Otolaryngology ◽

10.1186/s43163-021-00190-x ◽

2022 ◽

Vol 38 (1) ◽

Author(s):

R. Vignesh ◽

V. Nirmal Coumare ◽

S. Gopalakrishnan ◽

P. Karthikeyan

Keyword(s):

Otitis Media ◽

Platelet Rich Plasma ◽

Successful Outcome ◽

Control Group ◽

List Type ◽

Graft Material ◽

Group I ◽

Autologous Platelet Rich Plasma ◽

Conventional Myringoplasty ◽

Autologous Platelet

Abstract Background and aim Chronic otitis media is defined as a chronic inflammation of the middle ear cleft producing irreversible pathological changes. The myringoplasty aims at the reconstruction of the tympanic membrane using a graft material. Autologous platelet-rich plasma can be used along with the graft to have a successful outcome. The aim of the study was to assess the efficacy of autologous platelet-rich plasma on graft uptake in myringoplasty. Methods This was a randomized controlled trial with a sample size of 76 patients. All patients were above 18 and below 55 years of age diagnosed with chronic suppurative otitis media and were planned for myringoplasty. The participants were randomly allotted to two groups by block randomization (block of 4). Intra-operatively, group I (n=38) received platelet-rich plasma–soaked gel foam and group II (n=38) was taken as the control group who received saline-soaked gel foam and examined by a blinded examiner at the end of 1st and 3rd months. Results The mean air-bone gap reduction post-operatively in the platelet-rich plasma group was 8.68 ± 4.8 (P value 0.034) and 6.05 ± 4.05 in the control group. The improvement in pure-tone average in the platelet-rich plasma group (P = 0.009) is more than that in the control group. The graft uptake was higher among the platelet-rich plasma group than the control group both at 1st and 3rd months (P value 0.049) which were statistically significant. Conclusion The present study concludes that the usage of platelet-rich plasma in the conventional myringoplasty technique has improved the success rate of graft uptake and reduced the graft migration. Trial registration Clinical Trials Registry-India (ICMR-NIMS) CTRI/2020/04/024416. Date of registration: 01/04/2020. Date of enrolment of the first participant to the trial: 06/04/2020. URL of the trial registry: http://www.ctri.nic.in. Highlights Usage of autologous platelet-rich plasma (PRP) in conventional myringoplasty in underlay technique. Assessment of graft uptake, percentage of perforation closure, and the audiological outcome. Significant mean reduction of ABG in the PRP group. Significant improvement in PTA average in the PRP group. Graft uptake and percentage of perforation closure were higher in the PRP group and the control group. PRP is also beneficial in revision cases.

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ON IMPROVING THE TREATMENT OF PATIENTS WITH CHRONIC WOUNDS OF VENOUS GENESIS

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії ◽

10.31718/2077-1096.19.3.75 ◽

2019 ◽

Vol 19 (3) ◽

pp. 75-78

Author(s):

Ya.V. Rybalka

Keyword(s):

Wound Healing ◽

Chronic Wounds ◽

Platelet Rich Plasma ◽

Control Group ◽

Wound Area ◽

Area Reduction ◽

Autologous Platelet Rich Plasma ◽

Group 2 ◽

Autologous Platelet ◽

Group 1

Introduction. The analysis of the effectiveness of applying autologous platelet-rich plasma was performed in order to improve the results of surgical treatment of patients with wounds that heal slowly. Materials. The study is based on the analysis of the treatment of 61 patients who suffered from slow-healing wounds of venous genesis with a diameter of no more than 8 cm on the lower extremities and took the treatment at the surgical ward. The proposed method was based on obtaining autologous enriched platelet plasma followed by its injection along the periphery of the wound. The degree of reduction of the wound surface area was assessed by the method of contact planimetry per day. The patients of the 1st group demonstrated the wound area reduction by 5.1 ± 0.6%, and the patients of the group 2 – by 1.8 ± 0.4% (p <0005). The results indicate an increase in the rate of wound healing in patients in group 1 compared with patients in group 2. During the entire follow-up period (12 weeks), complete wound epithelialisation occurred in 21 (70%) patients in group 1 and in 10 (33.3%) patients of the group 2. The wound area decreased by more than 50% in 6 patients of group 1 (20%) and in 2 (7%) patients of the control group. The wound area decreased less than by 50% in 3 patients in group 1 (10%), and in 19 patients in group 2 (60%). The use of PRP therapy has been proven to have a significant positive effect on the process of wound healing compared with the use of standard methods used for the control group. Conclusion: increase in the wound healing pace in patients of group 1 (test) compared with patients of group 2 (control) can be explained by using autologous platelet-enriched plasma as an additional means to the standard therapy.

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