Polyextremotolerant Bacillus cereus groups isolated from Algerian arid ecosystems and bioprospecting for their bioactive compounds

2020 ◽  
Vol 9 (6) ◽  
pp. 222-228
Author(s):  
Waffa Bouali ◽  
Fikrettin Sahin ◽  
Nadia Aissaoui ◽  
Gonca Altin ◽  
Badis Mesli ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Bacillus Cereus ◽  
Arid Regions ◽  
Fatty Acid Analysis ◽  
Arid Ecosystems ◽  
Preliminary Screening ◽  
Fame Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Bacteria And Fungi

In previous work, 70 Bacillus sp. isolates originating from 4 soil plots from southwestern Algeria were studied for their poten al plant growth promoting (PGP). In this study, pH, organic ma er, texture, Calcium carbonate, and Electrical conduc vity of the soil samples were determined, 08 selected isolates were idenfied based on rRNA 16S and FAME analysis using GC. Antimicrobial activity against bacteria and fungi were also investigated. All the four soils were moderately Calcium carbonate, pH of soil was alkaline. Our results showed that the electrical conductivity was high for all soils and the four soil isolates in semi-arid and arid regions were poor in organic matter. In addition, Fatty acid analysis showed the presence of 17 di erent fa y acids. Three fa y acids, 15:0 iso, 16:0, and 17:0, 17:0 were present in all isolates. Thus, the isolates were classified into two clusters which were con rmed the results of 16SrRNA sequence analysis. Preliminary screening revealed that the Bacillus thuringiensis isolates showed strong an bacterial and an fungal proper es against S. aureus (between 7mm to 11mm) and A. niger (between 7mm to 8mm). The aim of the present study is the screening of eight Bacillus cereus strains isolated from four rhizosphere soil sample of South-Western region of Algeria province for their an microbial activity.

Film coating of seeds with Bacillus cereus RS87 spores for early plant growth enhancement

2008 ◽  
Vol 54 (10) ◽  
pp. 861-867 ◽  
Author(s):  
Kanchalee Jetiyanon ◽  
Sakchai Wittaya-Areekul ◽  
Pinyupa Plianbangchang
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Plant Height ◽  
Root Length ◽  
Film Coating ◽  
Field Trials ◽  
Growth Enhancement ◽  
Vegetative Cells ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacterium Bacillus cereus RS87 was previously reported to promote plant growth in various crops in both greenhouse and field trials. To apply as a plant growth promoting agent with practical use, it is essential to ease the burden of routine preparation of a fresh suspension of strain RS87 in laboratory. The objectives of this study were to investigate the feasibility of film-coating seeds with B. cereus RS87 spores for early plant growth enhancement and to reveal the indoleacetic acid (IAA) production released from strain RS87. The experiment consisted of the following 5 treatments: nontreated seeds, water-soaked seeds, film-coated seeds, seeds soaked with vegetative cells of strain RS87, and film-coated seeds with strain RS87 spores. Three experiments were conducted separately to assess seed emergence, root length, and plant height. Results showed that both vegetative cells and spores of strain RS87 significantly promoted (P ≤ 0.05) seed emergence, root length and plant height over the control treatments. The strain RS87 also produced IAA. In conclusion, the film coating of seeds with spores of B. cereus RS87 demonstrated early plant growth enhancement as well as seeds using their vegetative cells. IAA released from strain RS87 would be one of the mechanisms for plant growth enhancement.

scholarly journals Efficacy of Plant Growth-Promoting Bacteria Bacillus cereus YN917 for Biocontrol of Rice Blast

2021 ◽  
Vol 12 ◽  
Author(s):  
Hu Zhou ◽  
Zuo-hua Ren ◽  
Xue Zu ◽  
Xi-yue Yu ◽  
Hua-jun Zhu ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Rice Blast ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Detached Leaf ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus YN917, obtained from a rice leaf with remarkable antifungal activity against Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible biocontrol properties. YN917 strain exhibits multiple plant growth-promoting and disease prevention traits, including production of indole-3-acetic acid (IAA), ACC deaminase, siderophores, protease, amylase, cellulase, and β-1,3-glucanase, and harboring mineral phosphate decomposition activity. The effects of the strain YN917 on growth promotion and disease prevention were further evaluated under detached leaf and greenhouse conditions. The results revealed that B. cereus YN917 can promote seed germination and seedling plant growth. The growth status of rice plants was measured from the aspects of rice plumule, radicle lengths, plant height, stem width, root lengths, fresh weights, dry weights, and root activity when YN917 was used as inoculants. YN917 significantly reduced rice blast severity under detached leaf and greenhouse conditions. Genome analysis revealed the presence of gene clusters for biosynthesis of plant promotion and antifungal compounds, such as IAA, tryptophan, siderophores, and phenazine. In summary, YN917 can not only be used as biocontrol agents to minimize the use of chemical substances in rice blast control, but also can be developed as bio-fertilizers to promote the rice plant growth.

scholarly journals The Plant Growth–Promoting Rhizobacterium Bacillus cereus AR156 Induces Systemic Resistance in Arabidopsis thaliana by Simultaneously Activating Salicylate- and Jasmonate/Ethylene-Dependent Signaling Pathways

2011 ◽  
Vol 24 (5) ◽  
pp. 533-542 ◽  
Author(s):  
Dong-Dong Niu ◽  
Hong-Xia Liu ◽  
Chun-Hao Jiang ◽  
Yun-Peng Wang ◽  
Qing-Ya Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Signaling Pathways ◽  
Pseudomonas Syringae ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Dependent Manner ◽  
Challenge Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus AR156 is a plant growth–promoting rhizobacterium that induces resistance against a broad spectrum of pathogens including Pseudomonas syringae pv. tomato DC3000. This study analyzed AR156-induced systemic resistance (ISR) to DC3000 in Arabidopsis ecotype Col-0 plants. Compared with mock-treated plants, AR156-treated ones showed an increase in biomass and reductions in disease severity and pathogen density in the leaves. The defense-related genes PR1, PR2, PR5, and PDF1.2 were concurrently expressed in the leaves of AR156-treated plants, suggesting simultaneous activation of the salicylic acid (SA)- and the jasmonic acid (JA)- and ethylene (ET)-dependent signaling pathways by AR156. The above gene expression was faster and stronger in plants treated with AR156 and inoculated with DC3000 than that in plants only inoculated with DC3000. Moreover, the cellular defense responses hydrogen peroxide accumulation and callose deposition were induced upon challenge inoculation in the leaves of Col-0 plants primed by AR156. Also, pretreatment with AR156 led to a higher level of induced protection against DC3000 in Col-0 than that in the transgenic NahG, the mutant jar1 or etr1, but the protection was absent in the mutant npr1. Therefore, AR156 triggers ISR in Arabidopsis by simultaneously activating the SA- and JA/ET-signaling pathways in an NPR1-dependent manner that leads to an additive effect on the level of induced protection.

Suppression of southern corn leaf blight by a plant growth-promoting rhizobacterium Bacillus cereus C1L

2010 ◽  
Vol 157 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Chien-Jui Huang ◽  
Ken-Haow Yang ◽  
Yi-Hung Liu ◽  
Yu-Ju Lin ◽  
Chao-Ying Chen
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Leaf Blight ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Corn Leaf Blight

scholarly journals Functional and Genetic Diversity of Bacteria Associated with the Surfaces of Agronomic Plants

Plants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 91 ◽  
Author(s):  
Basharat Ali
Keyword(s):  
Genetic Diversity ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Host Plants ◽  
Human Pathogens ◽  
Positive Interaction ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

The main objective of this study was to evaluate the genetic diversity and agricultural significance of bacterial communities associated with the surfaces of selected agronomic plants (carrot, cabbage and turnip). The bacterial diversity of fresh agricultural produce was targeted to identify beneficial plant microflora or opportunistic human pathogens that may be associated with the surfaces of plants. Bacterial strains were screened in vitro for auxin production, biofilm formation and antibiotic resistance. 16S rRNA gene sequencing confirmed the presence of several bacterial genera including Citrobacter, Pseudomonas, Pantoea, Bacillus, Kluyvera, Lysinibacillus, Acinetobacter, Enterobacter, Serratia, Staphylococcus, Burkholderia, Exiguobacterium, Stenotrophomonas, Arthrobacter and Klebsiella. To address the biosafety issue, the antibiotic susceptibility pattern of strains was determined against different antibiotics. The majority of the strains were resistant to amoxicillin (25 µg) and nalidixic acid (30 µg). Strains were also screened for plant growth-promoting attributes to evaluate their positive interaction with colonized plants. Maximum auxin production was observed with Stenotrophomonas maltophilia MCt-1 (101 µg mL−1) and Bacillus cereus PCt-1 (97 µg mL−1). Arthrobacter nicotianae Lb-41 and Exiguobacterium mexicanum MCb-4 were strong biofilm producers. In conclusion, surfaces of raw vegetables were inhabited by different bacterial genera. Potential human pathogens such as Bacillus cereus, Bacillus anthracis, Enterobacter cloacae, Enterobacter amnigenus and Klebsiella pneumoniae were also isolated, which makes the biosafety of these vegetable a great concern for the local community. Nevertheless, these microbes also harbor beneficial plant growth-promoting traits that indicated their positive interaction with their host plants. In particular, bacterial auxin production may facilitate the growth of agronomic plants under natural conditions. Moreover, biofilm formation may help bacteria to colonize plant surfaces to show positive interactions with host plants.

scholarly journals Draft Genome Sequence of Bacillus cereus 905, a Plant Growth-Promoting Rhizobacterium of Wheat

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Haixia Ding ◽  
Ben Niu ◽  
Haiyan Fan ◽  
Yan Li ◽  
Qi Wang
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Coding Sequences ◽  
Wheat Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus 905 is a plant growth-promoting rhizobacterium, isolated from wheat rhizosphere. The draft genome sequence of this strain is 5.39 Mb and harbors 5,412 coding sequences.

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