scholarly journals Effect of Super Absorbent Application on Antioxidant Enzyme Activities in Canola ( Brassica napus L.) Cultivars under Water Stress Conditions

2009 ◽  
Vol 4 (3) ◽  
pp. 215-223 ◽  
Author(s):  
Shirani-R
2008 ◽  
Vol 53 (No. 12) ◽  
pp. 517-523 ◽  
Author(s):  
J. Csiszár ◽  
E. Lantos ◽  
I. Tari ◽  
E. Madoşă ◽  
B. Wodala ◽  
...  

We compared the enzymatic antioxidative defence mechanisms of some regional subspecies of <I>Allium (A. cepa </I>L., <I>A. ascalonicum</I> auct. hort., A.<I> sativum</I> L.) cultivated mainly in the western regions of Romania, and two modern Hungarian climate resistant F <sub>1</sub> hybrids. The variability in the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR) and glutathione S-transferase (GST) and their changes under soil moisture stress were investigated. 1-week-long water stress revealed that among three <I>Allium</I> species, relative water content decreased only in <I>A. ascalonicum</I> leaves (up to 16%). Unlike root enzymes, the activities of the shoot enzymes, especially POD, GR and GST showed a stronger correlation with the water content of the leaves after one week of water withdrawal; regression coefficients (<I>R</I><sup>2</sup>) were 0.359, 0.518 and 0.279, respectively. The ancient populations with elevated (or highly inducible) antioxidant enzyme activities may be interesting for further research and for breeding of new <I>Allium</I> varieties.


2010 ◽  
Vol 46 (No. 1) ◽  
pp. 27-34 ◽  
Author(s):  
T. Abedi ◽  
H. Pakniyat

The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.


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