scholarly journals Study of Hemolysin Gene “aspHS” and Its Phenotype in Aspergillus Fumigatus

2019 ◽  
Vol 7 (15) ◽  
pp. 2399-2403 ◽  
Author(s):  
Majid Zarrin ◽  
Farzaneh Ganj
Keyword(s):  
Early Diagnosis ◽  
Aspergillus Fumigatus ◽  
Gene Sequence ◽  
Restriction Enzymes ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Sheep Blood ◽  
Hemolysin Gene ◽  
Pcr Products

AIM: The main goal of this study was to analysis the “aspHS” gene and its phenotype in A. fumigatus. METHODS: Fifty-three A. fumigatus strains, including environmental, clinical and reference isolates, were used in this research. PCR was carried out based on Asp-hemolysin gene sequence. Two restriction enzymes TagI and NcoI were employed for digestion of PCR products. RESULTS: PCR products of 180 and 450 bp were generated for all A. fumigatus isolates. Digestion of the aspHS gene 180 bp amplicons with TagI and 450 bp amplicons with TagI and NcoI produced the expected bands for most isolates. Hemolysin production of A. fumigatus isolates was evaluated on sheep blood agar (SBA). CONCLUSION: In conclusion, our results provide evidence hemolysin activity and analysis of aspHS gene of A. fumigatus. These data may be useful in early diagnosis of A. fumigatus infections.

Rapid Streptococcal Tests

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 288-288
Author(s):  
S. DUBOSE RAVENEL ◽  
GREGORY CARL ELLIS ◽  
WILLIAM N. MICHAL
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Practice Setting ◽  
Throat Culture ◽  
Culture Techniques ◽  
Sheep Blood ◽  
Office Practice ◽  
Important Study

Roddey et al have reported an important study on the sensitivity and specificity of the Strep A OIA test compared with two culture techniques—5% sheep blood agar and Todd-Hewitt broth—in an office practice setting. They found the sensitivity and specificity of OIA as compared with sheep blood culture to be 91.4% and 95.6%, and compared with the broth method, 90.4% and 94.1%, respectively. They conclude that the OIA method is preferable for the majority of their patients, but recommend a throat culture be performed in cases with a negative OIA test.

Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India

2015 ◽  
Author(s):  
A. Banik ◽  
D. P. Isore ◽  
S. N. Joardar ◽  
K. Batabyal ◽  
S. Dey
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
West Bengal ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Enteropathogenic Escherichia Coli ◽  
Highly Pathogenic ◽  
E Coli ◽  
Sheep Blood ◽  
Common Serotypes

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. coli isolates from diarrhoeic and non-diarrhoeic dogs in Southern part of West Bengal. A total of 112 canine samples were tested during May to September 2012 revealing approx 63.4% (71) samples positive for Escherichia coli. The most common serotypes were O8 (23.9%) followed by O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) and O6 (11.3%). Among these O6, O8, O26 and O157 were highly pathogenic to mice causing almost 100% mortality within 24hrs of inoculation and were also detected to be haemolytic on sheep blood agar plates except serotype O8. These isolates were mostly sensitive to nalidixic acid (80.29%), cotrimoxazole (78.88%), ciprofloxacin (74.65%), colistin and ceftriaxone (both 71.83%) but were resistant to amikacin (97.18%), kanamycin (95.78%), cephalexin (92.96%) and enrofloxacin (84.51%).

scholarly journals Comparison of Various Culture Methods for Isolation of Group B Streptococcus from Intrapartum Vaginal Colonization

2013 ◽  
Vol 5 (01) ◽  
pp. 42-45 ◽  
Author(s):  
Kavitha P Konikkara ◽  
Shrikala Baliga ◽  
Suchitra M Shenoy ◽  
B Bharati
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
Culture Method ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Third Trimester ◽  
Sheep Blood ◽  
Vaginal Colonization ◽  
Vaginal Swabs ◽  
Group B

ABSTRACT Aims: Group B Streptococcus (GBS) is one of the most common causes of neonatal sepsis throughout the world. Reports of vaginal colonization of GBS in India are few and variable. A study was conducted on pregnant women in a tertiary care hospital to compare various methods for isolation of GBS, to study the prevalence of GBS in pregnant women in third trimester, and to determine risk factors for GBS colonization. Settings and Design: Observational descriptive study. Materials and Methods: High vaginal swabs from 150 pregnant women in their third trimester were used to compare three methods for isolation of GBS viz. direct culture on 5% Sheep Blood agar, direct culture on selective Columbia Blood Agar and culture in LIM enrichment broth with subsequent culture on 5% Sheep Blood agar. A history of associated risk factors was also taken. Statistical Analysis Used: Statistical analysis was performed by Chi–square test. Results: Isolation was best from LIM enrichment broth with subsequent culture on 5% Sheep Blood Agar. Prevalence of GBS colonization by using culture method was 12.67%. Most frequently associated risk factor was intrapartum fever (42.11%). Conclusions: Standard Culture Method using LIM enrichment should be adopted as standard practice for isolation of GBS from vaginal swabs.

scholarly journals PREVALENCE OF VIRULENCE FACTORS AMONG CLINICAL ISOLATES OF ENTEROCOCCUS SPP.

2016 ◽  
Vol 9 (9) ◽  
pp. 72 ◽  
Author(s):  
Umadevi Sivaraman ◽  
Ravichandran L ◽  
Pramodhini S ◽  
Srirangaraj S ◽  
Seetha Ks
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Enterococcus Faecium ◽  
Sheep Blood Agar ◽  
Clinical Isolates ◽  
Blood Agar ◽  
Sheep Blood ◽  
Biofilm Production ◽  
Enterococcus Spp

ABSTRACTObjective: To identify some of the virulence factors such as hemolysin, gelatinase, and biofilm production among the clinical isolates of enterococci.Methods: Hemolysin detection using sheep blood agar. Gelatine agar was used for gelatinase production, and tube adherence method was used fordetecting biofilm production.Results: Hemolysin production observed in 49% of isolates, gelatinase production in 41% of isolates, and 46% of isolates were produced biofilm.Conclusion: Virulence factors production was noticed more in Enterococcus faecalis than Enterococcus faecium. It is necessary to find theproduction of important virulence factors among the clinical isolates as they are always associated with virulence of the organism including drugresistance.Keywords: Hemolysin, Gelatinase, Biofilm, Enterococcus.

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